Article Per Year (5 Year)
p-Index From 2020 - 2025
0
P-Index
This Author published in this journals
All Journal Nexus Biomedika
Muhammad Luthfiyanto
Unknown Affiliation
Biochemistry, Genetics & Molecular Biology

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Pengaruh Elektroakupunktur Titik Zusanli (ST 36) dan Sanyinjiao (SP6) terhadap Ketebalan Korteks Adrenal Tikus (Rattus norvegicus) yang Dipapar Bising Intermiten Luthfiyanto, Muhammad; Muthmainah, .; Handayani, Selfi
Nexus Biomedika Vol 3, No 1 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: Noise exposure triggers stress and thickens adrenal cortex. Electroacupuncture on Zusanli (ST 36) and Sanyinjiao (SP 6) acupoints is known to reduce stress and improve the hypothalamic-pituitary-adrenal (HPA) axis. This study aimed to know the effect of electroacupuncture (EA) at Zusanli (ST 36) and Sanyinjiao (SP 6) acupoints towards adrenal cortex thickness of rats (Rattus norvegicus) exposed by intermittent noise. Methods: This study was a laboratory experimental study with the post-test only control group design. The subject of this study was male Wistar rats (Rattus norvegicus), 1-2 months old, and 200-250 grams of body weight. Twenty seven rats were taken by consecutive sampling. The rats were divided into 3 groups randomly, each group consisted of 9 rats. The Control Group (KK) was untreated, the treatment group 1 (KP1) was exposed by the noise, the treatment group 2 (KP2) was exposed by the noise and treated with electroacupuncture. Noise exposure was conducted for about 8 hours/day intermittently during 21 days respectively, while electroacupuncture treatment was done every 3 days, so there were 7 times of electroacupuncture treatment. Electroacupuncture was conducted at Zusanli (ST 36) and Sanyinjiao (SP 6) acupoints at 2 Hz of frequency in a continuous wave for 15 minutes that its intensity was adjusted until local muscle contractions were seen.  On the 22nd day, rats were sacrificed by neck dislocation and rat’s adrenal glands were taken and stained with Hematoxylin-Eosin for histological study. The thickness of rat’s adrenal cortex was observed under light microscope with 400x magnification. The data were analyzed by One Way ANOVA test and LSD test (α = 0.05). Results: The average of cortex adrenal thickness in KK,  KP1 and KP2 were 454.93 ± 2.03 µm, 565.59 ± 0.97 µm and 479.65 ± 1.34 µm, consecutively. One Way ANOVA test showed significant differences among the three groups with p=0.000 (p<0.05). LSD test also showed significant differences between KK-KP1, KK-KP2, KP1-KP2 with each of p=0.000 (p<0.05). Conclusion: Electroacupuncture at Zusanli (ST 36) and Sanyinjiao (SP 6) acupoints affected to prevent the thickening of adrenal cortex of rats (Rattus norvegicus) exposed by intermittent noise. Keywords: electroacupuncture, Zusanli (ST 36), Sanyinjiao (SP 6), noise, adrenal cortex’s thickness. 
Pengaruh Elektroakupunktur Titik Zusanli (ST 36) dan Sanyinjiao (SP6) terhadap Ketebalan Korteks Adrenal Tikus (Rattus norvegicus) yang Dipapar Bising Intermiten Muhammad Luthfiyanto; . Muthmainah; Selfi Handayani
Nexus Biomedika Vol 3, No 1 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Background: Noise exposure triggers stress and thickens adrenal cortex. Electroacupuncture on Zusanli (ST 36) and Sanyinjiao (SP 6) acupoints is known to reduce stress and improve the hypothalamic-pituitary-adrenal (HPA) axis. This study aimed to know the effect of electroacupuncture (EA) at Zusanli (ST 36) and Sanyinjiao (SP 6) acupoints towards adrenal cortex thickness of rats (Rattus norvegicus) exposed by intermittent noise. Methods: This study was a laboratory experimental study with the post-test only control group design. The subject of this study was male Wistar rats (Rattus norvegicus), 1-2 months old, and 200-250 grams of body weight. Twenty seven rats were taken by consecutive sampling. The rats were divided into 3 groups randomly, each group consisted of 9 rats. The Control Group (KK) was untreated, the treatment group 1 (KP1) was exposed by the noise, the treatment group 2 (KP2) was exposed by the noise and treated with electroacupuncture. Noise exposure was conducted for about 8 hours/day intermittently during 21 days respectively, while electroacupuncture treatment was done every 3 days, so there were 7 times of electroacupuncture treatment. Electroacupuncture was conducted at Zusanli (ST 36) and Sanyinjiao (SP 6) acupoints at 2 Hz of frequency in a continuous wave for 15 minutes that its intensity was adjusted until local muscle contractions were seen. On the 22nd day, rats were sacrificed by neck dislocation and rats adrenal glands were taken and stained with Hematoxylin-Eosin for histological study. The thickness of rats adrenal cortex was observed under light microscope with 400x magnification. The data were analyzed by One Way ANOVA test and LSD test (? = 0.05). Results: The average of cortex adrenal thickness in KK, KP1 and KP2 were 454.93 2.03 m, 565.59 0.97 m and 479.65 1.34 m, consecutively. One Way ANOVA test showed significant differences among the three groups with p=0.000 (p<0.05). LSD test also showed significant differences between KK-KP1, KK-KP2, KP1-KP2 with each of p=0.000 (p<0.05). Conclusion: Electroacupuncture at Zusanli (ST 36) and Sanyinjiao (SP 6) acupoints affected to prevent the thickening of adrenal cortex of rats (Rattus norvegicus) exposed by intermittent noise. Keywords: electroacupuncture, Zusanli (ST 36), Sanyinjiao (SP 6), noise, adrenal cortexs thickness.
Co-Authors . Muthmainah . Muthmainah Selfi Handayani