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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology Jurnal Ilmu Ternak Veteriner
H Maheshwari
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Agriculture, Biological Sciences & Forestry Veterinary

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The quality of spotted buffalo epididymal sperm with addition of raffinose as external cryoprotectant ., Yulnawati; ., Herdis; Maheshwari, H; Rizal, M
Indonesian Journal of Animal and Veterinary Sciences Vol 13, No 1 (2008)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (66.347 KB) | DOI: 10.14334/jitv.v13i1.592

Abstract

The aims of this research was to obtain the quality of Spotted buffalo epididymal sperm in different kind of extender in the three stages of cryopreservation (after dilution, post equilibration and post thawing). Spermatozoa was collected with combination of slicing and pressure method into the epididymal tissue in Andromedâextender. Soon after diluted and equilibrated, epididymal spermatozoa was cryopreserved in liquid nitrogen (-196°C).  The result showed that the percentage of motility after thawing in Andromedâ + raffinose 0.4% (47.0 ± 2.4%), was significantly different (P<0.05) from that of control (41.0 ± 2.0%), but there was no significantly different (P>0.05) from that of Andromedâ + raffinose 0.2% (46.0 ± 2.0%). The percentage of live sperm after thawing in control (52.2 ± 2.5%), was the lowest and significantly different (P<0.05) from that of Andromedâ + raffinose 0.2% (59.2 ± 2.6%) and Andromedâ + raffinose 0.4% (58.8 ± 3.1%). Moreover, the percentage of membrane integrity after thawing in control, Andromedâ + raffinose 0.2% and Andromedâ + raffinose 0.4% was 68.0 ± 1.1%; 67.2 ± 1.6% and 67.6 ± 1.2%, respectively. There was no significantly different (P>0.05) in the percentage of membrane integrity from all treatments. In conclusion, the addition of 0.2 and 0.4% raffinose into Andromedâ extender could improve the percentage of motility and viability of post thawing spotted buffalo epididymal spermatozoa. Key Words: Epididymal Sperm, Cryopreservation, Raffinose, Spotted Buffalo
Kualitas Semen Cair Asal Epididimis Kerbau Belang dalam Bahan Pengencer Andromed yang Mendapat Penambahan Sukrosa M Surachman; . Herdis; . Yulnawati; M Rizal; H Maheshwari
Media Peternakan Vol. 32 No. 2 (2009): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (162.972 KB)

Abstract

The aim of this research was to observe the quality of spotted buffalo epididymal sperm after storage at 4 °C as liquid semen for 12 and 24 hours in andromed containing sucrose 0.2% and 0.4%. Sperm was collected from epididymal tissues by combination of slicing and pressing methods. The parameters observed were the percentage of progressive motility and live sperm. The results showed that the percentage of progressive motility of the liquid semen after storage for 12 hours in andromed (A), andromed + sucrose 0.2% (P1) and andromed + sucrose 0.4% (P2) were 48.33%; 53.33% and 55% (P>0.05), respectively. In addition, the percentage of motility after 24 hours of storage in those extenders were 45%; 46.67% and 46.67% (P>0.05), respectively. The percentage of live sperm in A, P1 and P2 after 12 hours of storage were 70.33%; 73% and 73.33% while after 24 hours of storage, the percentage of live sperm in those extenders were 66.33%; 68.67% and 69.67%, respectively. In conclusion, the addition of 0.2% and 0.4% sucrose in andromed extender could maintain the quality of the spotted buffalo epididymal sperm after storage for 12 and 24 hours at 4 °C. Key words: epididymal sperm, spotted buffalo, sucrose, liquid semen, andromed
The Success Rate of Artificial Insemination Using Post-Thawed Spotted Buffaloes Epididymal Sperm Y Yulnawati; H Maheshwari; M Rizal; A Boediono
Media Peternakan Vol. 36 No. 2 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (420.561 KB) | DOI: 10.5398/medpet.2013.36.2.101

Abstract

Spotted buffalo, an exotic species that exists in Tana Toraja, South Sulawesi, Indonesia, is getting extinct due to high number of slaughtered during a funeral ceremony, called Rambu Solo’, as well as special treatments that do not allow the male spotted buffaloes perform natural mating activity. According to that, the research was trying to start conservation program by collected the cauda epididymal sperm soon after slaughtered. Two egg yolk-based extenders with different buffers, tris hydroxyl amino methane (TEY20) and trisodium citrate dehydrate (CEY20), were used as comparison to evaluate the post-thawed epididymal sperm quality and fertilizing capacity in artificial insemination program. The results showed that the post-thawed progressive motility of epididymal sperm was 40% and 39.17%, while viability was 65.99% and 63.26% and membrane integrity was 65.43% and 63.03% in TEY20 and CEY20 extenders, respectively. The success rate of pregnancy was 46.67% using post-thawed epididymal sperm in TEY20 and 40% using the one in CEY20 extenders. In conclusion, tris-based and citrate-based extenders have similar ability to maintain the epididymal sperm quality and its fertilizing capacity.
The quality of spotted buffalo epididymal sperm with addition of raffinose as external cryoprotectant Yulnawati .; Herdis .; H Maheshwari; M Rizal
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 1 (2008): MARCH 2008
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (66.347 KB) | DOI: 10.14334/jitv.v13i1.592

Abstract

The aims of this research was to obtain the quality of Spotted buffalo epididymal sperm in different kind of extender in the three stages of cryopreservation (after dilution, post equilibration and post thawing). Spermatozoa was collected with combination of slicing and pressure method into the epididymal tissue in Andromedâextender. Soon after diluted and equilibrated, epididymal spermatozoa was cryopreserved in liquid nitrogen (-196°C).  The result showed that the percentage of motility after thawing in Andromedâ + raffinose 0.4% (47.0 ± 2.4%), was significantly different (P<0.05) from that of control (41.0 ± 2.0%), but there was no significantly different (P>0.05) from that of Andromedâ + raffinose 0.2% (46.0 ± 2.0%). The percentage of live sperm after thawing in control (52.2 ± 2.5%), was the lowest and significantly different (P<0.05) from that of Andromedâ + raffinose 0.2% (59.2 ± 2.6%) and Andromedâ + raffinose 0.4% (58.8 ± 3.1%). Moreover, the percentage of membrane integrity after thawing in control, Andromedâ + raffinose 0.2% and Andromedâ + raffinose 0.4% was 68.0 ± 1.1%; 67.2 ± 1.6% and 67.6 ± 1.2%, respectively. There was no significantly different (P>0.05) in the percentage of membrane integrity from all treatments. In conclusion, the addition of 0.2 and 0.4% raffinose into Andromedâ extender could improve the percentage of motility and viability of post thawing spotted buffalo epididymal spermatozoa. Key Words: Epididymal Sperm, Cryopreservation, Raffinose, Spotted Buffalo
Co-Authors . Herdis . Yulnawati A Boediono Herdis . M Rizal M Surachman Y Yulnawati Yulnawati .