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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology Hemera Zoa Jurnal Ilmu Ternak Veteriner Animal Production : Indonesian Journal of Animal Production
A Boediono
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Veterinary

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The use of CR1aa for ovine in vitro embryo production ., Yulnawati; Setiadi, M.A; Boediono, A
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 2 (2006)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (153.531 KB) | DOI: 10.14334/jitv.v11i2.517

Abstract

The aim of this study was to investigate the capacity of CR1aa as a simple medium for maturation, fertilization and culture of ovine embryo in vitro. Oocytes were collected by slicing method in Phosphate Buffer Saline (PBS) supplemented with 5% Fetal Bovine Serum (FBS) and 100 IU/ml penicillin streptomycin. Oocytes were matured in Tissue Culture Medium (TCM)-199 as control or CR1aa as treatment medium. Both maturation medium were supplemented with 10% Fetal Bovine Serum (FBS), 10 IU/ml Follicle Stimulating Hormone (FSH), 10 IU/ml Luteinizing Hormone (LH), 1 μg/ml Estradiol and 100 IU/ml penicillin-streptomycin. Oocytes were incubated in 5% CO2 incubator, 38˚C for 24 h. Matured oocytes were fertilized in BO or CR1aa medium, supplemented with 2.5 mM caffeine benzoate and 20 mg /ml heparin. After 18 h in vitro fertilization, oocytes were cultured in TCM-199 or CR1aa medium, both supplemented with 5% FBS, 5 mg/ml insulin and 100 IU/ml penicillin streptomycin. Results showed that the highest maturation rate was found in TCM-199 medium (73.27%) and significantly different (P<0.05) from CR1aa (52.88%). Fertilization rate in CR1aa medium (67.59%) was higher (P<0.05) than in BO medium (52.94%). Furthermore, there was no significant difference (P>0.05) between cleavage rate of ovine embryos in TCM-199 and CR1aa medium (39.45% vs 50.94%). In conclusion, optimum result on ovine in vitro embryo production can be achieved from a combination of TCM-199 as maturation medium and CR1aa as fertilization and culture medium. Key Words: CR1aa, TCM-199, Embryo, Ovine
Viability of bovine demi embryo after splitting of fresh and frozen thawed embryo derived from in vitro embryo production Imron, M; Boediono, A; Supriatna, I
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 2 (2007)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (48.55 KB) | DOI: 10.14334/jitv.v12i2.549

Abstract

In vivo embryo production was limited by number of donor, wide variability respond due to superovulation program and also immunoactifity of superovulation hormone (FSH). Splitting technology could be an alternative to increase the number of transferrable embryos into recipien cows. Splitting is done with cutting embryo becoming two equal pieces (called demi embrio) base on ICM orientation.  The objective of this research was to determine the viability of demi embryo obtained from embryo splitting of fresh and frozen thawed embryo. The results showed that demi embryos which performed blastocoel reexpansion 3 hours after embryo splitting using fresh and frozen thawed embryos were 76.9 and 76.2% respectively. Base on existention of inner cell mass (ICM), the number of demi embryos developed with ICM from fresh and frozen thawed embryos were not significantly different (90.6 and 85.7% respectively. The cell number of demi embryo from fresh embryos splitting was not different compared with those from frozen thawed embryos (36.1 and 35.9 respectively). These finding indicated that embryo splitting can be applied to frozen thawed embryos with certain condition as well as fresh embryos. Key Words: In Vitro Embryo, Splitting, Demi Embryo, Cell Number
The Histological of Ewe Ovarium Post-Intrauterine Transplantation to Pseudopregnancy Rabbit Sumarmin, R; Boediono, A; Winarto, A; Yusuf, TL
ANIMAL PRODUCTION Vol 10, No 2 (2008): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (429.226 KB)

Abstract

The objective of this study was to evaluate the histologycal of ewe ovarium post-intrauterin transplantation to pseudopregnancy rabbit. The experiment was concerned with the 1 or 7 days of pseudopregnancy to receive the ewe ovarian transplant. Post transplantation 5, 7 or 9 days of ewe ovarium were recollected. To determine histologically post-intrauterine transplantation of ewe ovarium, the histological preparat was prepared by the paraffin methods followed by HE staining. The result showed that there were follicle development with all stages of follicle dynamics (Primordial, Primary, Prentral and Antral follicle stages) in all group of treatment. The ewe ovarian post-intrauterine transplantation damaged was found as follicles degeneration, ephitellial damaged and the formation of protein aggregate. It can be concluded that the histological of ewe ovarium post-intrauterine transplantation in pseudopregnancy rabbit is still good in the 5 or 7 days after intrauterine transplantation in pseudopregnancy rabbit. (Animal Production 10(2): 78-84 (2008) Key Words: Histological,  post-intrauterine transplantation, ewe ovarium
Viabilility of Rams Epididymal Sperm after Preservation in Low Temperature (5oc) Rizal, M; Herdis, Herdis; Boediono, A
ANIMAL PRODUCTION Vol 6, No 1 (2004): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (120.363 KB)

Abstract

The purpose of this research was to evaluate the viability of ram epididymal sperm collected from fresh caudal epididymis (H-0) or after storage in low temperature (5oC, in refrigerator) for one (H-1), two (H-2), and three (H-3) days.  Collected sperm were diluted in modified Tris extender and they were preserved in refrigerator up to four days.  The viability of diluted sperm was evaluated daily base on motility and sperm live.  Results indicated that mean sperm concentration after sperm diluted with 0.05 ml Tris extender of caudal epididymis was 2745 million/ml. Sperm motility and percentage of live for H-0 (71.25% and 82.83%) and H-1 (70.00% and 79.17%) were significantly higher (P<0.05) than H-2 (61.25% and 69.83%) and H-3 (51.67% and 66.17%).  Percentages of sperm motility and live of diluted sperm and preserved in refrigerator for H-0 were significantly higher (P<0.05) than H-1, H-2, and H-3.  These results showed that epididymal sperm collected from caudal epididymis up to three days of preservation (without further storage of the diluted sperm) could be used for artificial insemination or in vitro fertilization programs.  Diluted sperm of H-0 and H-1 could be preserved in refrigerator for two days and H-2 for one day. (Animal Production 6(1): 30-36 (2004) Key Words: Epididymal Sperm, Viability, Rams
Keasaman Cairan Tubuh dan Rasio Kelamin Anak Domba Garut (Ovis aries) yang Diberi Kation-Anion Ransum yang Berbeda F Fathul; T Toharmat; I G Permana; A Boediono
Media Peternakan Vol. 31 No. 2 (2008): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (186.385 KB)

Abstract

The objective of this research was to determine the effects of dietary cation-anion difference (DCAD) on acidity of ration, blood, vaginal mucous, and urine, number of fetus, number of offspring, and offspring sex ratio. The treatments were rations with DCAD -28, -18, 0, +14, and +32 meq which were offered to 15 ewes. This research used randomized complete block design. There were three groups, (I) ewes previously had twin female offsprings; (II) ewes previously had twin male offsprings; and (III) ewes previously had twin male and female offsprings. Results of this study showed that there were no differences observed for blood's pH, vaginal liquid's pH, offspring number, and offspring sex ratio. Increasing DCAD affected ration's pH, urine's pH, and number of fetus for each ewe. Blood's acidity was not affected by DCAD, but DCAD -28 meq reduced acid of blood, metabolic acidosis, and urine; the number of fetus and offspring. DCAD -28 meq might be anions excessive in the ration. DCAD 0 meq was relatively the best of ration because gave the normality of blood, metabolic, and urine, also relatively the highest of fetus and offspring. The offspring sex ratio related to vaginal mucose pH when artificial insemination was applied (r= 0.75) and ration's pH (r=0.47). Ewes previously had twin female lambs gave the number of fetus and offspring sex ratio were 2.00+0.71 and 0%, respectively. Ewes previously had twin male lambs gave the number of fetus and offspring sex ratio were 2.80+1.30 and 15.00+8.50%, respectively. Ewes previously had twin male and female lambs gave the number of fetus and offspring sex ratio were 1.60+1.14 and 61.75+31.50%, respectively.Key words: DCAD, pH, offspring sex ratio, lamb, ewe
Mineral Utilization in Rams Fed Ration Supplemented with Different Levels of Chromium, Calcium, and Cation-Anion Balances D Sudrajat; T Toharmat; A Boediono; I G Permana; R I Arifiantini; F Amir
Media Peternakan Vol. 34 No. 3 (2011): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (334.745 KB) | DOI: 10.5398/medpet.2011.34.3.212

Abstract

Chromium (Cr) is an essential mineral for ruminants. Its metabolism and interactions with other minerals has not been widely known. This experiment was designed to evaluate the utilization of minerals and growth of Garut ram fed ration supplemented with Cr and different Dietary Cation Anion Balance (DCAB) and Ca level. Dietary treatments, namely: R0 (Ration with DCAB+14); R1 (Ration with DCAB+14 + Cr 3ppm,); R2 (Ration with DCAB 0 + Ca); R3 (Ration with DCAB 0 + Cr 3 ppm + Ca), were allocated in twenty four of 1.5-2 years old Garut rams in a randomized block design. The results showed that Cr supplementation in rations containing different levels of Ca did not affect feed intake, body weight gain, and dry matter digestibility, but reduced the absorption of Cr and  Ca of the low Ca diet. Supplementation of Cr had no effect on Cr, Ca, Zn, and Mg status in blood and semen of the rams. Level of Cr intake had negative correlation with Ca absorption and positive correlation with blood Cr levels. There is a positive relationship between level of Ca intake with Ca and Mg absorption and blood Ca and Zn levels. Intake of Cr and Ca was not related to the semen Cr and Ca levels. 
The Success Rate of Artificial Insemination Using Post-Thawed Spotted Buffaloes Epididymal Sperm Y Yulnawati; H Maheshwari; M Rizal; A Boediono
Media Peternakan Vol. 36 No. 2 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (420.561 KB) | DOI: 10.5398/medpet.2013.36.2.101

Abstract

Spotted buffalo, an exotic species that exists in Tana Toraja, South Sulawesi, Indonesia, is getting extinct due to high number of slaughtered during a funeral ceremony, called Rambu Solo’, as well as special treatments that do not allow the male spotted buffaloes perform natural mating activity. According to that, the research was trying to start conservation program by collected the cauda epididymal sperm soon after slaughtered. Two egg yolk-based extenders with different buffers, tris hydroxyl amino methane (TEY20) and trisodium citrate dehydrate (CEY20), were used as comparison to evaluate the post-thawed epididymal sperm quality and fertilizing capacity in artificial insemination program. The results showed that the post-thawed progressive motility of epididymal sperm was 40% and 39.17%, while viability was 65.99% and 63.26% and membrane integrity was 65.43% and 63.03% in TEY20 and CEY20 extenders, respectively. The success rate of pregnancy was 46.67% using post-thawed epididymal sperm in TEY20 and 40% using the one in CEY20 extenders. In conclusion, tris-based and citrate-based extenders have similar ability to maintain the epididymal sperm quality and its fertilizing capacity.
Roles of Dietary Cobalt and Administration of Mixed Rumen Bacteria in Regulating Hematological Parameters of Pre-weaning Twin Lambs T Adelina; A Boediono; I G Permana; T R Wiradarya; D Evvyernie; T Toharmat
Media Peternakan Vol. 36 No. 2 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (308.07 KB) | DOI: 10.5398/medpet.2013.36.2.126

Abstract

Cobalt (Co) is required by rumen microorganism for vitamin B12 synthesis. Vitamin B12 is an important cofactor for methionine synthesis and gluconeogenesis. In young ruminants up to 6–8 wk old, the rumen has not been completely developed and rumen microorganisms are not ready to supply vitamin B12. The aim of this research was to determine the potency of mixed rumen bacteria and dietary supplementation of Co and its effect on plasma glucose, blood minerals (Co, Fe, and Zn) concentrations, and hematology of pre-weaning twin lambs. Twelve one month-old local twin lambs were assigned to 4 groups in a randomized complete block design. Lambs were fed cow milk at 10% body weight, adjusted weekly for 80 d. Mixed rumen bacteria were offered at 15 mL/d (8.295x1010 cfu). Dietary treatments were: 1) basal diet (Control), 2) basal diet + 1 mg/kg DM cyanocobalamin (VitB12) and 3) basal diet + 1 mg/kg DM of Co + administration of 15 mL mixed rumen bacteria (CoBac). There were no treatment effects on neither plasma glucose and blood mineral concentrations nor hematological profiles. This study demonstrated that pre-weaning twin lambs are not responsive to supplementation of Co and administration of mixed rumen bacteria.
PCS-14 Potential Reproductive Adaptation of Mice (Mus musculus) from Mild Stress using Dark Light Cycle Alteration D N Pristihadi; M Fakhrudin; N M D Haq; A Boediono
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (510.081 KB)

Abstract

Stress regarded as a major cause of body destruction. Stress can trigger the gluconeogenesis mechanism that initiates new glucose production from another molecule in the body’s storage tissue depot. When the stress occurs, the body gives a ‘fight or flight’ response. In this condition, the body prioritizes to survive rather than multiplying itself. The reproductive system categorized as the highest rank in the body needs pyramid. Therefore, the reproductive system is on the first line to be sacrificed when the stress occurs.This research was conducted to observe the potential mice's reproductive adaptation from mild stress using dark light cycle alteration. Mice considered as the small laboratory animal with rapid metabolism rate. This study expected to be able to enrich the information of mice’s biology reproductive adaptation.
The use of CR1aa for ovine in vitro embryo production Yulnawati .; M.A Setiadi; A Boediono
Jurnal Ilmu Ternak dan Veteriner Vol 11, No 2 (2006): JUNE 2006
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (153.531 KB) | DOI: 10.14334/jitv.v11i2.517

Abstract

The aim of this study was to investigate the capacity of CR1aa as a simple medium for maturation, fertilization and culture of ovine embryo in vitro. Oocytes were collected by slicing method in Phosphate Buffer Saline (PBS) supplemented with 5% Fetal Bovine Serum (FBS) and 100 IU/ml penicillin streptomycin. Oocytes were matured in Tissue Culture Medium (TCM)-199 as control or CR1aa as treatment medium. Both maturation medium were supplemented with 10% Fetal Bovine Serum (FBS), 10 IU/ml Follicle Stimulating Hormone (FSH), 10 IU/ml Luteinizing Hormone (LH), 1 μg/ml Estradiol and 100 IU/ml penicillin-streptomycin. Oocytes were incubated in 5% CO2 incubator, 38˚C for 24 h. Matured oocytes were fertilized in BO or CR1aa medium, supplemented with 2.5 mM caffeine benzoate and 20 mg /ml heparin. After 18 h in vitro fertilization, oocytes were cultured in TCM-199 or CR1aa medium, both supplemented with 5% FBS, 5 mg/ml insulin and 100 IU/ml penicillin streptomycin. Results showed that the highest maturation rate was found in TCM-199 medium (73.27%) and significantly different (P<0.05) from CR1aa (52.88%). Fertilization rate in CR1aa medium (67.59%) was higher (P<0.05) than in BO medium (52.94%). Furthermore, there was no significant difference (P>0.05) between cleavage rate of ovine embryos in TCM-199 and CR1aa medium (39.45% vs 50.94%). In conclusion, optimum result on ovine in vitro embryo production can be achieved from a combination of TCM-199 as maturation medium and CR1aa as fertilization and culture medium. Key Words: CR1aa, TCM-199, Embryo, Ovine
Co-Authors A Winarto D Sudrajat Diah Nugrahani Pristihadi Dwierra Evvyernie F Amir F Fathul H Maheshwari herdis herdis I G Permana I Supriatna M Fakhrudin M Imron M Rizal M.A Setiadi N M D Haq R I Arifiantini R Sumarmin, R T adelina T R Wiradarya T Toharmat TL Yusuf, TL Y Yulnawati Yulnawati .