Amiruddin Dg. Malewa
Faculty Of Animal Husbandry And Fishery, Tadulako University

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Journal : AGRISAINS

Studi Morfologi Domba Ekor Gemuk Silangan (DEGS) Di Lembah Palu Irfan, Mohamad; Dg. Malewa, Amiruddin
AgriSains Vol 13, No 3 (2012)
Publisher : FAPETKAN UNTAD

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Abstract

This Research target to know the comparison form and size measure of parts of body (morfologi) of Fat Tail Sheep Livestock Traverse (female and Masculine DEGS) residing in Countryside of Bora and Chief Of Village Taipa Sulawesi Province. Parameter of livestock body qualitative and quantitative which Method used in this research is combination between "Frequency Relative" as which used by Mulliadi (1996) and Analyse The Especial Component (Principal Component Analyse) according to Gasperz (1992). Research result indicate that qualitative and quantitative sheep livestock in Countryside Bora more dominant form and also size measure of body compared to a sheep residing in Chief of village Taipa, this matter  strenghtened with the result analyse the especial component (AKU) (P>0,05) do not give the real influence among second of accurate sheep. that way also size measure parameter at female sheep like head footage, long  ear, long neck, long  body, long  back, long  thigh and tail length do not give the real influence (P>0,05). Key words : DEGS, morfologi.
Growth Hormone Gene Polymorphism of Donggala Sheep Dg. Malewa, Amirudin; Hakim, Lukman; Maylinda, Sucik
AgriSains Vol 20, No 1 (2019)
Publisher : FAPETKAN UNTAD

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Abstract

The study aimed to know the gene polymorphism of growth hormone (GH) of Donggala sheep as potential candidate gene that control the growth characteristics. A total of 50 sheeps from farm community localities (Palu, Central Sulawesi) were analyzed laboratory in BIOMOL FMIPA UB through PCR-RFLP with HinfI enzyme. Results obtained the genotypes of Donggala sheep for PCR-RFLP Hinf1 with genotype AA, AB, BC are 0,933, 0,044 and 0,022. The frequency of A, B and C alleles for Donggala sheep are 0.967, 0.022 and 0.011. PIC value (Polymorphic Information Content) of Donggala sheep is 6.5%. Although PIC value is low, PCR-RFLP Hinf1 finders still have a polymorphic allele variety. Sequence of the three PCR samples also found polymorphisms at the nucleotide base level after comparing them with Lohi breed sheep sequences. Sequence comparisons have a similarity of 97%. Mutations for Donggala sample (20) occurred 16 points while those for Donggala sample (57) occured for 26 points. The point of mutation in the nucleotide base is the cause of polymorphism.Keyword: Donggala sheep, GH genes, PCR-RFLP, Hinf1.