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All Journal Microbiology Indonesia QUANTUM: Jurnal Inovasi Pendidikan Sains Jurnal Ilmu Kefarmasian Indonesia
Sogandi Sogandi
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Biochemistry, Genetics & Molecular Biology Chemistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Physics Public Health

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PENGGUNAAN LKS DALAM STRATEGI PEMBELAJARAN TTW (THINK TALK WRITE) TERHADAP HASIL BELAJAR SISWA PADA MATERI REAKSI REDOKS Sogandi Sogandi; Rilia Iriani; Yudha Irhasyuarna
QUANTUM: Jurnal Inovasi Pendidikan Sains Vol 10, No 2 (2019): Oktober 2019
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/quantum.v10i2.5850

Abstract

Penelitian ini bertujuan: (1) untuk mengetahui perbedaan hasil belajar kognitif antara siswa yang menggunakan strategi pembelajaran TTW berbantuan LKS dengan siswa yang menggunakan strategi pembelajaran TTW tanpa berbantuan LKS pada materi reaksi redoks; (2) mengetahui respon siswa terhadap penerapan strategi pembelajaran TTW (Think Talk Write). Metode penelitian ini adalah eksperimen semu. Sampel penelitian ini adalah siswa kelas X yang berjumlah 69 orang. Rancangan penelitian yang digunakan adalah The nonequivalent control group design. Pengumpulan data menggunakan tes hasil belajar dan angket respon. Data dianalisis menggunakan uji-t dengan taraf signifikansi 5%. Teknik persentase digunakan untuk analisis respon. Perhitungan uji-t menunjukkan bahwa t-hitung > t-tabel sehingga disimpulkan bahwa terdapat perbedaan hasil belajar yang signifikan antara kelas eksperimen dan kontrol. Sedangkan untuk respon siswa sebanyak 81,47% memberikan respon positif pada kelas eksperimen dan 73,89% siswa memberikan respon positif pada kelas kontrol.
DNA Barcoding Tanaman Mangga Kasturi (Mangifera casturi) Asal Kalimantan Selatan Berbasis DNA Kloroplas Gen rbcL dan matK Sogandi Sogandi; Zuraida Sagala
JURNAL ILMU KEFARMASIAN INDONESIA Vol 20 No 1 (2022): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35814/jifi.v20i1.1045

Abstract

This study aims to see the genetic diversity of the musk mango (Mangifera casturi) plant in South Kalimantan based on DNA barcode techniques using sequences from the rbcL gene and the matK gene. The plant samples used in this study were the leaves of the musk mango (Mangifera casturi) taken from eight regencies and cities in South Kalimantan Province, namely from Amuntai, Astambul, Barito Kuala, Peat, Kandanga, Babirik, Tabalong, and Tanah Laut. Isolation and DNA analysis of the musk mango plant was carried out in the research laboratory of the Faculty of Biotechnology, Atma Jaya Catholic University of Indonesia, Jakarta. The analysis of the genetic diversity of the musk mango began with the stage of amplifying genomic DNA (gDNA) using rbcL and matK primers. The results of the DNA isolation samples showed a high concentration level of 17-91 ng/µL with a purity of 1.72 -1.92 ng/µL. When PCR was carried out using rbcL and matK primers, from 8 areas in South Kalimantan, 5 samples (Babirik, Tabalong, Kandangan, Amuntai, Batola, Peat, and Tanah Laut) were amplified with rbcL primers and 6 samples (Tanah Laut, Babirik, Tabalong , Amuntai, Peat, and Kandangan) amplified with matK primer. The relationship between the musk mango in South Kalimantan can be seen from the phylogenetic tree.
Inhibitory activity of Lactobacillus plantarum U10 isolated from Tempoyak (fermented durian) Made in Indonesia against Salmonella typhi SOGANDI SOGANDI; APON ZAENAL MUSTOPA; I MADE ARTIKA; BUGI RATNO BUDIARTO
Microbiology Indonesia Vol. 9 No. 2 (2015): June 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1138.504 KB) | DOI: 10.5454/mi.9.2.5

Abstract

Lactobacillus plantarum U10 produced bacteriocin U10 which was isolated from a traditionally fermented food “tempoyak” from Sumatera Island in Indonesia. Production of the bacteriocins started at early exponential phase and reached maximum level at early stationary phase. Furthermore, plantaricins U10 was purified by ammonium sulphate precipitation followed by gel filtration chromatography. L. plantarum U10 produced two bacteriocins with a molecular mass of approximately 4.5 and 9.8 kDa by SDS-PAGE.  The mode of action of plantaricins U10 was identified as bactericidal agents against Salmonella typhi ATCC25241 as proven by CFU counting and SEM micrographs that showed differences in cell structures between treated cells and the non-treated control. SEM examination also confirmed structural destruction of membrane cells integrity and considerable morphological alteration of S.typhi.
Co-Authors APON ZAENAL MUSTOPA BUGI RATNO BUDIARTO I MADE ARTIKA Rilia Iriani Yudha Irhasyuarna Zuraida Sagala