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M.B. Malole
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Agriculture, Biological Sciences & Forestry

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White Spot Disease in Black Tiger Shrimp (Penaeus Monodon Fab.) : Infection of White Spot Virus at 20, 100 and 200 uG/Ml by Dipping Method within 120 Minutes Exposure Time Alifuddin, M.; Dana, D.; Eidman, M.; Malole, M.B.; Pasaribu, F.H.
Jurnal Akuakultur Indonesia Vol. 2 No. 1 (2003): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (164.31 KB) | DOI: 10.19027/jai.2.31-35

Abstract

An infection trial of white spot disease on tiger shrimp was conducted by water borne inoculation with the different of  viral concentration. The result showed that the viral concentration influenced the appearance of white spot specific lesion, occurrence and disease development. This study also indicated that white spot virus infect hepato-pancreatic tissue as well as gill, intestine and lymphoid tissues. Key word: White spot virus, infectious disease, tiger shrimp, Penaeus monodon Fab.   ABSTRAK Percobaan penularan penyakit white spot pada udang windu telah dilakukan  secara perendaman dengan berbagai konsentrasi virus.  Hasil percobaan ini memperlihatkan, bahwa konsentrasi virus mempengaruhi kemunculan lesi spesifik penyakit white spot, kejadian dan perkembangan penyakit.  Percobaan ini juga memperlihatkan, bahwa hepatopankrea terserang virus white spot disamping, insang, usus dan jaringan limfoid. Kata kunci: Virus white spot, penyakit infeksi, udang windu, Penaeus monodon Fab.
Patogenesis of White Spot Virus Infection (WSV) on Black Tiger Shrimp (Penaeus Monodon Fab.) Alifuddin, M.; Dana, D.; Eidman, M.; Malole, M.B.; Pasaribu, F.H.
Jurnal Akuakultur Indonesia Vol. 2 No. 2 (2003): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1535.76 KB) | DOI: 10.19027/jai.2.85-92

Abstract

The pathogenesis determination of white spot disease on tiger shrimp to study effect of various white spot virus concentrations on pathological changes was conducted by water borne infection with the several of viral concentration within 60 minute using black tiger shrimp post larvae. The result showed the incubation period of the disease based on appeareance of white spot at 2-2000 ng/ml is 17 days p.i., whilst at 0.2-0.02 ng/ml is 19 days p.i. It seem, the lowest viral concentration stimulated respond immune of shrimp which indicated by low mortalities percentage (13.3 and 10.6%). Stage of disease development recognized throughout histological analyse, based on hypertropie of nuclei, is stage 0-4. Based on microscopic observation, the white spot seen pleomorphic forms which related to its development and seem to be used as rapid diagnostic tool for indicating of WS infection. This study also proved by histological observation that hepatopancreatic tissue can be infected as well as epidermis of carapace, gill, intestine and lymphoid tissues. One of pathological changes by WS infection is white spot as pathogonomic lesi of the disease. Keyword : White spot virus, infectious disease, tiger shrimp (Penaeus monodon Fab.)
The Improvement of Protein Retention through a Higher Utilization of Carbohydrate in the Diet with Chromium for Common Carp Cyprinus carpio Linn. S, Nuryati; Dana, D.; Malole, M.B.; Pasaribu, F.H.
Jurnal Akuakultur Indonesia Vol. 3 No. 2 (2004): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (323.452 KB) | DOI: 10.19027/jai.3.43-46

Abstract

Primary shrimp cell culture from lymphoid organ of Penaeus monodon was successfully developed. Minced tissues of lymphoid organ were seeded by tripsin enzyme and culture in Leibovit'z-15 supplemented with 10% fetal bovine serum and 5% NaCl. Plates were then incubated at 27 °C with 5% C02 supply. Confluent cells were infected by white spot baculovirus (WSBV). Cytopathic effect (CPE) induced by white spot baculovirus showed characteristics of detachment cells and lysis forming giant polycaryon cells. Primary shrimp cell culture could be continued by anvanced cell culture resulting cell line could be used virus media. Key words : cell culture, lymphoid organ, tiger shrimp, invitro, virus   ABSTRAK Kultur sel primer dari organ limfoid udang windu Penaaeus monodon telah berhasil dilakukan. Potongan organ limfoid dipisahkan menjadi sel-sel tunggal dengan menggunakan enzim tripsin. Sel-sel tunggal tersebut dikuitur dalam media Leibovit'z-15 dengan tambahan 10 % fetal bovine serum dan 5 % NaCl. Biakan diinkubasi pada suhu 27 °C dengan kandungan C02 sebanyak 5 %. Biakan yang telah konfluen diinfeksi dengan virus WSBV dan menghasilkan cytopathic effect (CPE) dengan karakter sel lepas dari perlekatan dan mengalami lisis yang menyisakan giant cells dengan inti banyak (polycaryons). Kultur sel primer ini dapat ditindaklanjuti dengan kultur sel dengan pasase yang berulang-ulang sehingga dapat menghasilkan sel lestari (cell line) untuk media tumbuh virus. Kata kunci: biakan sel, organ limfoid, udang windu, invitro, virus
Co-Authors D. Dana F.H. Pasaribu M. Alifuddin M. Eidman Nuryati S