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Physical Inactivation of Monodon Baculovirus (Mbv), a Pathogenic Virus of Tiger Prawn (Penaeus Monodon Fab.) Alifuddin, M.
Jurnal Akuakultur Indonesia Vol. 1 No. 1 (2002): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACTA study of physical inactivation of MBV was carried out by conducting monitoring observation of reared shrimp test under laboratory condition. Experimental shrimp were reared at PSIK (Pusat Studi Ilmu Kelautan), Jakarta and examined histologically for MBV infection at Lab. of Fish Health, Department of Aquaculture Faculty of Fisherise IPB. This study was conducted by transmission trial and physical inactivation of virus MBV. Preparation of inoculum followed Momoyama and Sano (1988); shrimp test were infected by water borne infection. Presence of infection indicated by histological observation of hipertropied hepatopancreas cell containing inclusion bodies of virus. Inactivation of MBV was done by heating at 40, 45, 50 and 55 'C for 30 minute and uv radiation for 5, 10, 15 and 20 minute with the distance 30 cm from the uv lamp 15 watt as radiation sources. Transmission trial showed that infection occured 6 hours post inoculation and inclusion bodies were detected at day 5th; showed the virus lost their infectivities or virulent since no inclusion t Ddies as indicator for MBV infection were detected on hepatopancreas of shrimp test. Key words : Tiger prawn (Penaeus monodon Fab.), viral diseases, physical inactivation.ABSTRAKSuatu penelitian mengenai inaktifasi fisik terhadap Monodon baculovirus (MBV), suatu virus patogen yang menyerang udang windu (Penaeus monodon Fab.), telah dilaksanankan sejak bulan Juli 1994 sampai bulan Maret 1995. Penelitian ini dilakukan di Laboratorium Kesehatan Ikan, Jurusan Budidaya Perairan, Fakultas Perikanan IPB dan Pusat Studi limu Kelautan (PSIK) Institut Pertanian Bogor, Jakarta. Percobaan yang dilakukan meliputi percobaan penularan virus dan percobaan inaktifasi fisik virus. Inaktifasi fisik terhadap virus MBV dilakukan dengan pemanasan pada 4 tingkat suhu yang berbeda (40, 45, 50 dan 55 'C) selama 30 menit dan radiasi ultraviolet pada 4 tingkat waktu penyinaran (5, 10, 15 dan 20 menit) dengan jarak 30 cm dari sumber radiasi lampu uv 15 watt. Penyiapan inokulum dilakukan mengikuti Momoyama dan Sano (1 988), inokulasi dilakukan dengan "water borne infection". Pemeriksaan infeksi MBV pada udang qii dilakukan secara histologis dengan memperhatikan hipertropi sel hepatopankreas. Dari penelitian ini terungkap, bahwa infeksi virus secara laboratoris membutuhkan waktu 6 jam post inokulasi dan badan inklusi MBV terlihat pada hari ke-5 yang terus berkembang sampai akhir penelitian; sedangkan inaktifasi fisik baik dengan pemanasan maupun radiasi ultraviolet menyebabkan virus kehilangan infektivitasnya yang ditunjukkan dengan tidak ditemukannya badan inklusi sebagai indikator adanya infeksi dan perkembangan virus MBV. Kata kunci : Udang windu (Penaeus monodon Fab.), penyakit viral, inaktifasi fisik

Immunostimulan on Aquatic Organisms Alifuddin, M.
Jurnal Akuakultur Indonesia Vol. 1 No. 2 (2002): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

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Parasites Inventory on Ornamental Fish Transported in Soekarno-Hatta Airport, Cengkareng, Jakarta Alifuddin, M.; Priyono, A.; Nurfatimah, A.
Jurnal Akuakultur Indonesia Vol. 1 No. 3 (2002): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACTStudy of parasites inventory on ornamental fish transported in Soekarno-Hatta Airport, Cengkareng, Jakarta was done. Parasites were identified from coral platy fish (Xiphophorus maculatus), guppy cobra fish (Poecilia reticulata), red nose tetra fish (Hemigrammus rhodostomus) and serpe minor fish (Hyphessobrycon serpae). Parasites found from coral platy fish were identified as Dactylogyrus and Argulus japonicus; Trichodina heterodentata and Lerneae infected guppy cobra fish; red nose tetra fish was infected by Gyrodactylus, whilst Ichthyoph-thirius multifiliis were found in serpe minor fish only. All of the parasites were known as ectoparasites and excluded from the List of Pest and Parasite Fish Quarantine. From this study, there was a correlation between present of parasites with length of fish.Key words : Ornamental fish, fish parasites, fish quarantine ABSTRAKPenelitian inventarisi parasit pada ikan hias yang dilalulintaskan melalui Bandara Soekarno-Hatta, Cengkareng, Jakarta telah dilakukan. Inventarisasi parasit dilakukan terhadap ikan platis koral (Xyphophorus maculatus), ikan gupi kobra (Poecilia reticulata), ikan red nose tetra (Hemgrammus rhodostomus) dan ikan serpe minor (Hyphessobrycon serpae). Pada ikan platis koral ditemukan parasit Dactylogyrus dan Argulus japonicus; pada ikan gupi kobra ditemukan parasit Trichodina heterodentata dan Lerneae; pada ikan red nose tetra hanya ditemukan parasit Gyrodactylus dan pada ikan serpe minor hanya ditemukan parasit Ichthyophthirius multifiliis. Semua parasit yang ditemukan tergolong ektoparasit dan tidak tergolong sebagai patogen karantina. Dari penelitian ini terlihat adanya hubungan keberadaan parasit dengan ukuran panjang ikan.Kata kunci : Ikan hias, parasit ikan, karantina ikan.

White Spot Disease in Black Tiger Shrimp (Penaeus Monodon Fab.) : Infection of White Spot Virus at 20, 100 and 200 uG/Ml by Dipping Method within 120 Minutes Exposure Time Alifuddin, M.; Dana, D.; Eidman, M.; Malole, M.B.; Pasaribu, F.H.
Jurnal Akuakultur Indonesia Vol. 2 No. 1 (2003): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

An infection trial of white spot disease on tiger shrimp was conducted by water borne inoculation with the different of viral concentration. The result showed that the viral concentration influenced the appearance of white spot specific lesion, occurrence and disease development. This study also indicated that white spot virus infect hepato-pancreatic tissue as well as gill, intestine and lymphoid tissues. Key word: White spot virus, infectious disease, tiger shrimp, Penaeus monodon Fab. ABSTRAK Percobaan penularan penyakit white spot pada udang windu telah dilakukan secara perendaman dengan berbagai konsentrasi virus. Hasil percobaan ini memperlihatkan, bahwa konsentrasi virus mempengaruhi kemunculan lesi spesifik penyakit white spot, kejadian dan perkembangan penyakit. Percobaan ini juga memperlihatkan, bahwa hepatopankrea terserang virus white spot disamping, insang, usus dan jaringan limfoid. Kata kunci: Virus white spot, penyakit infeksi, udang windu, Penaeus monodon Fab.

Patogenesis of White Spot Virus Infection (WSV) on Black Tiger Shrimp (Penaeus Monodon Fab.) Alifuddin, M.; Dana, D.; Eidman, M.; Malole, M.B.; Pasaribu, F.H.
Jurnal Akuakultur Indonesia Vol. 2 No. 2 (2003): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

The pathogenesis determination of white spot disease on tiger shrimp to study effect of various white spot virus concentrations on pathological changes was conducted by water borne infection with the several of viral concentration within 60 minute using black tiger shrimp post larvae. The result showed the incubation period of the disease based on appeareance of white spot at 2-2000 ng/ml is 17 days p.i., whilst at 0.2-0.02 ng/ml is 19 days p.i. It seem, the lowest viral concentration stimulated respond immune of shrimp which indicated by low mortalities percentage (13.3 and 10.6%). Stage of disease development recognized throughout histological analyse, based on hypertropie of nuclei, is stage 0-4. Based on microscopic observation, the white spot seen pleomorphic forms which related to its development and seem to be used as rapid diagnostic tool for indicating of WS infection. This study also proved by histological observation that hepatopancreatic tissue can be infected as well as epidermis of carapace, gill, intestine and lymphoid tissues. One of pathological changes by WS infection is white spot as pathogonomic lesi of the disease. Keyword : White spot virus, infectious disease, tiger shrimp (Penaeus monodon Fab.)

Parasites in Fresh Water Ornamental Fish (Cupang, Guppy and Rainbow Fish) Alifuddin, M.; Hadiroseyani, Yani; Ohoiulun, I.
Jurnal Akuakultur Indonesia Vol. 2 No. 2 (2003): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Parasite inventory on some fresh water ornamental fish was done by survey methode. Parasites found from cupang fish namely Trichodinid (Ciliophora), Dactylogyrus sp. and Gyrodaclylus sp. (Platyhelminthes), Acanthocephala and cystic form; in guppy fish Trichodinid (Ciliophora), Gyrodaclylus sp. (Platyhelminthes) and Lerneae sp. (Crustasea); on rainbowg found Trichodinid (Ciliophora), Dactylogyrus sp., Gyrodaclylus sp. (Platyhelminthes), Acanthocephala, Lerneae sp. (Krustasea) and cystic form. Parasites found known as ecto, meso and endoparasites. From this study, there is correlation between parastes present with length fish. Key word : Fish water ornamental fish, fish parasites ABSTRAK Inventarisi parasit telah dilakukan dengan metode survey pada ikan hias air tawar yakni, ikan cupang (Betta splendens Regan), ikan gapi (Poecilia reticulata Peters) dan ikan rainbow (Melanotaenia macculochi Ogilby). Pada ikan cupang ditemukan parasit Trichodinid (Ciliophora), Dactylogyrus sp. dan Gyrodaclylus sp. (Platy-helminthes), Acanthocephala dan kiste); pada ikan gapi ditemukan Trichodinid (Ciliophora), Gyrodaclylus sp. (Platyhelminthes) dan Lerneae sp. (Krustasea); pada ikan rainbowg ditemukan parasit Trichodinid (Ciliophora), Dactylogyrus sp., Gyrodaclylus sp. (Platyhelminthes), Acanthocephala, Lerneae sp. {Krustasea) dan kista. Parasit yang ditemukan tergolong ekto, meso dan endoparasit. Dari penelitian ini terlihat adanya hubungan keberadaan parasit dengan ukuran panjang ikan. Kata kunci: Ikan hias air tawar, parasit ikan

Effect of Ultraviolet Radiation on Pathogenity of White Spot Syndrome Virus in Giant Tiger Prawn (Penaeus monodon Fab.) Subkhan, M.; Alifuddin, M.; Taslihan, A.
Jurnal Akuakultur Indonesia Vol. 4 No. 1 (2005): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

This experiment was conducted to examine the effect of UV irradiation on pathogenity of white spot syndrome virus (WSSV) in black tiger shrimp (Penaeus monodon Fab.). A hundred ml of WSSV virus suspension (200 μg/ml) were placed at 30 cm under UV light 10 Watt. Radiation on WSSV virus was performed for 15, 30, 45, and 60 minutes. Black tiger sjrimp in density of 260 tails/L were immersed in 1000 ml of irradiated virus suspension (20 μg/ml) to test their pathogenities. The results of study showed that duration of UV irradiation on WSSV virus was reverse correlated to their pathogenities. In constrast, survival of black tiger shrimp was linear correlated to duration of UV inactivation of virus. Higher survival rate of shrimp (65.52%) after challenge test was obtained by irradiation of virus for 60 min. Keywords: WSSV, virus, pathogen, ultraviolet, black tiger prawn, Penaeus monodon ABSTRAK Penelitian ini dilakukan untuk mengetahui pengaruh radiasi UV terhadap patogenitas virus White Spot Syndrome Virus (WSSV) pada udang windu (Penaeus monodon Fab). Suspensi virus WSSV dengan konsentrasi 200 μg/ml sebanyak 100 ml ditempatkan 30 cm diradiasi menggunakan UV 10 Watt. Radiasi dilakukan selama 15, 30, 45, dan 60 menit. Udang dengan kepadatan 260 ekor/L direndam dalam 1000 ml suspensi virus (20 μg/ml) hasil radiasi untuk menguji patogenitasnya. Hasil penelitian menunjukkan bahwa lama radiasi UV pada virus WSSV berbanding terbalik dengan tingkat patogenitasnya. Sementara itu, lama inaktivasi virus dengan UV berbanding lurus terhadap kelangsungan hidup udang. Kelangsungan hidup udang windu tertinggi (65,52%) setelah uji tantang diperoleh dengan meradiasi virus selama 60 menit. Kata kunci: WSSV, virus, patogen, ultraviolet, udang windu, Penaeus monodon

Potential of Chromolaena odorata Leaf as A Cure of Aeromonas hydrophila on Giant Gouramy (Osphronemus gouramy) Hadiroseyani, Y.; Hafifuddin, .; Alifuddin, M.; Supriyadi, H.
Jurnal Akuakultur Indonesia Vol. 4 No. 2 (2005): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

This study was conducted to examine the potency of Chromolaena odorata leaf extract as a medicine for skin eruption disease caused by Aeromonas hydrophila in giant gouramy Osphronemus gouramy. Leaf extract of Chromolaena odorata for in vitro test was 0 (as control), 13000, 15000, 17000, 19000 and 21000 ppm, poured onto TSA medium containing bacteria 103 cfu/ml, and then is incubated for 24 hours. In vivo test was performed by injecting bacteria 0.1 ml of 109 cfu/ml intramuscularly into giant gouramy (14 g weight), and then fish were maintained in the water containing 15000 ppm of Chromolaena odorata leaf extract. In vitro study showed that prevention area of leaf extract against Aeromonas hydrophila was increase by increasing the concentration of leaf extract used, reached 9,33 mm. Prevention zone of leaf extract by difusion tends to constant, reached 7,6 mm. By in vivo test, survival rate of giant gouramy infected by Aeromonas hydrophila was no significantly different between dosages of leaf extract. All treated fish, excluded control died after 24 hours infection. Keywords: Aeromonas hydrophila, Osphronemus gouramy, Chromolaena odorata ABSTRAK Penelitian ini dilakukan untuk mengetahui potensi daun kirinyuh Chromolaena odorata sebagai obat untuk penyakit cacar yang diakibatkan oleh Aeromonas hydrophila pada ikan gurame Osphronemus gouramy. Konsentrasi ekstrak daun Chromolaena odorata untuk uji in vitro adalah 13000, 15000, 17000, 19000 dan 21000 serta 0 ppm sebagai kontrol, yang diletakkan di atas media TSA yang telah mengandung biakan bakteri 103 cfu/ml dan diinkubasi selama 24 jam. Uji in vivo dilakukan dengan menginjeksikan bakteri sebanyak 0,1 ml (109 cfu/ml) secara intramuskular ke ikan gurame (berat 14 g) dan kemudian ikan dipelihara dalam air yang mengandung ekstrak daun kirinyuh 15000 ppm. Hasil uji in virto menunjukkan bahwa semakin tinggi konsentrasi ekstrak daun kirinyuh basah semakin efektif dalam menghambat perkembangan A. hydrophila dengan zona hambat tertinggi mencapai 9,33 mm. Zona hambat yang dihasilkan melalui metode difusi cenderung konstan, mencapai 7,6 mm. Melalui uji in vivo, tingkat kelangsungan hidup ikan gurame yang tidak berbeda nyata pada masing-masing perlakuan, bahkan terjadi kematian total dalam 24 jam pada semua perlakuan, kecuali kontrol. Kata kunci: Aeromonas hydrophila, Osphronemus gouramy, Chromolaena odorata

Effect of Heating at Various Temperatures for 30 Minutes on Pathogenicity of White Spot Syndrome Virus (WSSV) in Tiger Prawn (Penaeus monodon Fabr.) Priatni, D.; Alifuddin, M.; Djokosetiyanto, D.
Jurnal Akuakultur Indonesia Vol. 5 No. 1 (2006): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

White spot syndrome virus (WSSV) is a strong pathogenic virus which spread very rapidly and can cause tiger shrimp mass mortality within a short period. Enhancement of shrimp immunity by infecting inactivated WSSV is one of the efforts to overcome WSSV infection in shrimp. In this study, inactivated WSSV were prepared by heating them with various temperatures namely 45, 50, 55 and 60oC for 30 minutes. The results shows that infection with heating inactivated WSSV at 45°C and 60°C for 30 min on PL-15 could increase their immunities. The survival rate of inactivated WSSV-infected shrimp after challenge test with WSSV virulent reached 77%, while no survive shrimp was observed in control. This suggests that shrimp immunity could be improved by vaccination using WSSV virus inactivated by heating. Keywords: WSSV virus, pathogen, tiger shrimp, heating ABSTRAK White Spot Syndrome Virus (WSSV) merupakan virus yang sangat ganas bagi udang windu, dengan penularan yang sangat cepat dan menyebabkan kematian dalam waktu yang cepat. Peningkatan imunitas udang dengan meenginfeksikan WSSV inaktif merupakan salah satu upaya untuk menanggulangi infeksi WSSV pada udang. Pada penelitian ini, inaktivasi WSSV dilakukan menggunakan pemanasan pada suhu berbeda, yaitu 45, 50, 55 dan 60oC selama 30 menit. Hasil penelitian menunjukkan bahwa infeksi PL-15 menggunakan virus WSSV yang telah diinaktivasi dengan pemanasan pada suhu 45oC dan 60oC dapat meningkatkan daya tahan udang. Kelangsungan hidup udang yang telah diinfeksi dengan WSSV hasil inaktivasi sebelum uji tantang dengan WSSV virulen mencapai 77%, sementara udang yang tidak diinfeksi dengan WSSV hasil inaktivasi adalah semua mati. Dengan demikian dapat disimpulkan bahwa daya tahan udang dapat ditingkatkan melalui vaksinasi menggunakan WSSV yang telah diinaktivasi dengan pemanasan. Kata kunci: virus WSSV, patogen, udang windu, pemanasan

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