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F.H. Pasaribu
Faculty of Veterinary Medicine, Bogor Agriculture Institute, Bogor, Indonesia
Agriculture, Biological Sciences & Forestry

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White Spot Disease in Black Tiger Shrimp (Penaeus Monodon Fab.) : Infection of White Spot Virus at 20, 100 and 200 uG/Ml by Dipping Method within 120 Minutes Exposure Time Alifuddin, M.; Dana, D.; Eidman, M.; Malole, M.B.; Pasaribu, F.H.
Jurnal Akuakultur Indonesia Vol. 2 No. 1 (2003): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (164.31 KB) | DOI: 10.19027/jai.2.31-35

Abstract

An infection trial of white spot disease on tiger shrimp was conducted by water borne inoculation with the different of  viral concentration. The result showed that the viral concentration influenced the appearance of white spot specific lesion, occurrence and disease development. This study also indicated that white spot virus infect hepato-pancreatic tissue as well as gill, intestine and lymphoid tissues. Key word: White spot virus, infectious disease, tiger shrimp, Penaeus monodon Fab.   ABSTRAK Percobaan penularan penyakit white spot pada udang windu telah dilakukan  secara perendaman dengan berbagai konsentrasi virus.  Hasil percobaan ini memperlihatkan, bahwa konsentrasi virus mempengaruhi kemunculan lesi spesifik penyakit white spot, kejadian dan perkembangan penyakit.  Percobaan ini juga memperlihatkan, bahwa hepatopankrea terserang virus white spot disamping, insang, usus dan jaringan limfoid. Kata kunci: Virus white spot, penyakit infeksi, udang windu, Penaeus monodon Fab.
Patogenesis of White Spot Virus Infection (WSV) on Black Tiger Shrimp (Penaeus Monodon Fab.) Alifuddin, M.; Dana, D.; Eidman, M.; Malole, M.B.; Pasaribu, F.H.
Jurnal Akuakultur Indonesia Vol. 2 No. 2 (2003): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1535.76 KB) | DOI: 10.19027/jai.2.85-92

Abstract

The pathogenesis determination of white spot disease on tiger shrimp to study effect of various white spot virus concentrations on pathological changes was conducted by water borne infection with the several of viral concentration within 60 minute using black tiger shrimp post larvae. The result showed the incubation period of the disease based on appeareance of white spot at 2-2000 ng/ml is 17 days p.i., whilst at 0.2-0.02 ng/ml is 19 days p.i. It seem, the lowest viral concentration stimulated respond immune of shrimp which indicated by low mortalities percentage (13.3 and 10.6%). Stage of disease development recognized throughout histological analyse, based on hypertropie of nuclei, is stage 0-4. Based on microscopic observation, the white spot seen pleomorphic forms which related to its development and seem to be used as rapid diagnostic tool for indicating of WS infection. This study also proved by histological observation that hepatopancreatic tissue can be infected as well as epidermis of carapace, gill, intestine and lymphoid tissues. One of pathological changes by WS infection is white spot as pathogonomic lesi of the disease. Keyword : White spot virus, infectious disease, tiger shrimp (Penaeus monodon Fab.)
The Improvement of Protein Retention through a Higher Utilization of Carbohydrate in the Diet with Chromium for Common Carp Cyprinus carpio Linn. S, Nuryati; Dana, D.; Malole, M.B.; Pasaribu, F.H.
Jurnal Akuakultur Indonesia Vol. 3 No. 2 (2004): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (323.452 KB) | DOI: 10.19027/jai.3.43-46

Abstract

Primary shrimp cell culture from lymphoid organ of Penaeus monodon was successfully developed. Minced tissues of lymphoid organ were seeded by tripsin enzyme and culture in Leibovit'z-15 supplemented with 10% fetal bovine serum and 5% NaCl. Plates were then incubated at 27 °C with 5% C02 supply. Confluent cells were infected by white spot baculovirus (WSBV). Cytopathic effect (CPE) induced by white spot baculovirus showed characteristics of detachment cells and lysis forming giant polycaryon cells. Primary shrimp cell culture could be continued by anvanced cell culture resulting cell line could be used virus media. Key words : cell culture, lymphoid organ, tiger shrimp, invitro, virus   ABSTRAK Kultur sel primer dari organ limfoid udang windu Penaaeus monodon telah berhasil dilakukan. Potongan organ limfoid dipisahkan menjadi sel-sel tunggal dengan menggunakan enzim tripsin. Sel-sel tunggal tersebut dikuitur dalam media Leibovit'z-15 dengan tambahan 10 % fetal bovine serum dan 5 % NaCl. Biakan diinkubasi pada suhu 27 °C dengan kandungan C02 sebanyak 5 %. Biakan yang telah konfluen diinfeksi dengan virus WSBV dan menghasilkan cytopathic effect (CPE) dengan karakter sel lepas dari perlekatan dan mengalami lisis yang menyisakan giant cells dengan inti banyak (polycaryons). Kultur sel primer ini dapat ditindaklanjuti dengan kultur sel dengan pasase yang berulang-ulang sehingga dapat menghasilkan sel lestari (cell line) untuk media tumbuh virus. Kata kunci: biakan sel, organ limfoid, udang windu, invitro, virus
Hematology of common carp following DNA vaccination and koi herpesvirus challenge test Nuryati, Sri; Maswan, N.A.; Alimuddin, .; Sukenda, .; Sumantadinata, K.; Pasaribu, F.H.; Soejoedono, R.D.; Santika, A.
Jurnal Akuakultur Indonesia Vol. 9 No. 1 (2010): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (194.781 KB) | DOI: 10.19027/jai.9.9-15

Abstract

The study was aimed to determine the effectiveness of DNA vaccine doses on hematological aspect which represent immune response and its influence on common carp survival rate. DNA vaccines encoding the viral glycoprotein of  koi herpesvirus (KHV) have been proved to highly protect the fish under laboratory condition.  A dose of 12.5 µg/100 µl vaccine had resulted in a survival rate of 96.67 % during 30 days after challenge test with a lethal dose of KHV. Fish vaccinated using lower doses, i.e. 2.5 and 7.5 µg/100µl showed 100% mortality after 15 and 19 days challenge test respectively, whereas non vaccinated fish as a control showed 100% mortality after 17 days challenge test.  Total leucocytes of the vaccinated fish were higher than control until 42 days post vaccination, but declined afterward.  Phagocytic index of the vaccinated fish using 12.5 µg/100 µl was declined after 49 days post vaccination or 7 days post challenge test. Key words: DNA vaccine, Koi herpesvirus (KHV), leucocyte, phagocytic index, Cyprinus carpio   ABSTRAK Penelitian ini bertujuan untuk menguji pengaruh vaksinasi menggunakan vaksin DNA dengan dosis berbeda terhadap gambaran darah ikan sebagai respresentasi tanggap kebal ikan mas serta pengaruhnya terhadap tingkat kelangsungan hidup ikan mas. Vaksin DNA penyandi glikoprotein koi herpesvirus (KHV) dapat memberikan proteksi yang tinggi pada percobaan skala laboratorium.  Vaksinasi dengan dosis 12,5 µg/100µl dapat mempertahankan kelangsungan hidup sebesar 96,67% selama satu bulan setelah uji tantang dengan virus KHV menggunakan dosis letal.  Ikan yang divaksin dengan dosis yang lebih rendah yaitu 2,5 dan 7,5 µg/100µl mengalami kematian total berturut-turut setelah 15 dan  19 hari uji tantang, sedangkan ikan kontrol yang tidak divaksin mengalami kematian total setelah 17 hari uji tantang.  Jumlah leukosit total ikan yang divaksinasi lebih tinggi dibanding dengan kontrol sampai hari ke-42, setelah itu mengalami penurunan.  Indeks fagositosis ikan yang divaksin dengan dosis 12,5 µg/100µl mengalami penurunan setelah hari ke-49 atau 7 hari setelah uji tantang. Kata kunci: Vaksin DNA, Koi herpesvirus (KHV), leukosit, indeks fagositosis, Cyprinus carpio
Co-Authors A. Santika Alimuddin D. Dana K. Sumantadinata M. Alifuddin M. Eidman M.B. Malole N.A. Maswan Nuryati S R.D. Soejoedono Sri Nuryati Sukenda .