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All Journal Jurnal MIPA Jurnal Bios Logos Wacana, Jurnal Sosial dan Humaniora AGRI-SOSIOEKONOMI COCOS
Jeany Mandang
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Economics, Econometrics & Finance Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Physics Social Sciences

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Journal : Jurnal Bios Logos

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Keragaman Genetik Beberapa Varietas Kentang (Solanum tuberosum L.) Berdasarkan Penanda Random Amplified Polimorphic DNA (RAPD) (Genetic Diversity of Several Varieties of Potato (Solanumtuberosum L) Based on Random Amplified Polymorphic DNA (RAPD)) Kawengian, Yefta B; Lengkong, Edy; Mandang, Jeany
JURNAL BIOS LOGOS Vol 6, No 2 (2016): JURNAL BIOSLOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.6.2.2016.13794

Abstract

Abstrak Pengembangan tanaman kentang (Solanum tuberosum L.)  unggul untuk menunjang kebutuhan produksi kentang yang terus meningkat membutuhkan tersedianya informasi genetik tanaman kentang yang ada. Informasi keragaman genetik dapat diperoleh menggunakan penanda molekuler RAPD yang dapat mendeteksi keragaman sampai pada tingkat DNA, baik pada daerah penyandi atau bukan penyandi protein dengan cara mendeteksi polimorfik sekuens nukleotida. Informasi yang diperoleh akurat karena tidak dipengaruhi lingkungan.  Penelitian ini bertujuan untuk menganalisis keragaman genetik dari kentang kultivar Superjhon, Atlantik, Dessire, Nadia dan Granola menggunakan penanda RAPD.  Hasil penelitian menunjukkan bahwa dari 14 primer acak yang digunakan hanya 7 yang memberikan pola pita DNA yang polimorfik dan 16 pita dari total 28 pita DNA yang dihasilkan (57 %)  merupakan pita DNA polimorfik. Rata-rata keragaman genetik kentang sebesar 26,8 %. Keragaman genetik terkecil (15,4 %) adalah antara kentang  Atlantic dan Superjhon, sedangkan keragaman terbesar (57,7 %) antara kentang Nadia dan Dessire. Hasil analisis pengelompokan menunjukkan tanaman mengelompok berdasarkan sifat/karakter dan asalnya. Kata kunci: kentang, keragaman genetik, RAPD Abstract The information of potato genetic diversity are required to support the increasing potato demands in the superior potato production. The information of genetic diversity can be obtained using RAPD molecular marker. RAPD can detect the genetic diversity at the DNA level, both in the coding region and non-protein-coding regions by detecting polymorphic sequences in nucleotides. This method provide accurate genetic information because it is not influenced by the environment. This study was conducted to analyze the genetic diversity of potato Superjhon, Atlantic, Dessire, Nadia and Granola using RAPD marker. Amongst the 14 random primers, only 7 primers produced polymorphic banding pattern. Sixteen DNA bands of total 28 existed DNA bands (57%) were polymorphic. The average of genetic diversity was 26.8 %. The smallest genetic diversity (15.4%) was between Atlantic and Superjhon, whereas the greatest genetic diversity (57.7%) was between Nadia and Dessire. The analysis results showed that potato clustered grouping was based on their characters and their origins. Keywords: genetic diversity, potatoes, RAPD
Substitusi Media Murashige dan Skoog/MS dengan Air Kelapa dan Pupuk Daun Majemuk pada Pertumbuhan Anggrek Dendrobium secara in vitro (In Vitro Growth of Dendrobium Orchids under Substitution Murashige dan Skoog/MS Medium With Coconut Water and Compound Leaf Fertilizer) Inkiriwang, Annatje E.B.; Mandang, Jeany; Runtunuwu, Semuel
JURNAL BIOS LOGOS Vol 6, No 1 (2016): JURNAL BIOSLOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.6.1.2016.16258

Abstract

Abstrak  Penelitian ini bertujuan  untuk  menentukan  konsentrasi  substitusi air kelapa dan pupuk   daun  majemuk  yang tepat    pada   media  MS   terhadap   pertumbuhan   anggrek Dendrobium secara in vitro. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 7 perlakuan dan 10 kali ulangan.  Variabel yang diamati adalah persentase eksplan yang bertunas, jumlah tunas, jumlah daun, tinggi tanaman dan jumlah akar. Hasil penelitian menunjukkan substitusi media MS 50 %, air kelapa 30 % dan pupuk daun majemuk (Growmore®) 1,5 g/L menghasilkan rata-rata persentase eksplan yang bertunas sebesar 6,78 %; jumlah tunas sebesar 1,26 dan tinggi tanaman sebesar 1,20 cm.Kata kunci: air kelapa, in vitro, media MS, pupuk daun majemuk  Abstract             This research was aimed to determine the proper concentration of substitution of coconut water and compound leaf fertilizer in Murashige dan Skoog (MS) medium on the in vitro growth of Dendrobium orchids. The completely randomized design experiment consisted of 7 treatments and 10 replicates. The measured parameters were the percentage of sprouted explants, number of shoots, number of leaves, plant height and number of roots. The result showed that the substitution of 50% media MS, 30% coconut water and 1.5 g/L leaf fertilizer (Growmore®) resulted the growth of shoot explant as much as 6.78% the shoot number of 1.26 and 1.20 cm plant height .Keywords: coconut water, compound leaf fertilizer, in vitro, MS medium 
Co-Authors Agus Suman Deanne Kojoh Deanne Kojoh, Deanne Edy Lengkong Inkiriwang, Annatje E.B. Kaurow, Tamariska Julita Kawengian, Yefta B Marhaenus J. Rumondor Meklin Bakar Meklin Bakar, Meklin Rinny Mamarimbing Runtunuwu, Semuel saartje Sompotan Soemarno Soemarno Sofia Demasabu Sofia Demasabu, Sofia Sundalangi, Grace Treesye I Turang Wiske Rotinsulu