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All Journal Jurnal MIPA Jurnal Bios Logos Wacana, Jurnal Sosial dan Humaniora AGRI-SOSIOEKONOMI COCOS
Jeany Mandang
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Economics, Econometrics & Finance Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Physics Social Sciences

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Journal : AGRI-SOSIOEKONOMI

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Perlakuan Air Kelapa Tua, Dan BAP Pada Media MS, VW Terhadap Protocorm Anggrek Dendrobium sp. Secara Kultur In vitro Sundalangi, Grace; Mandang, Jeany; Sompotan, Saartje
AGRI-SOSIOEKONOMI Vol. 19 No. 1 (2023): Agri-Sosioekonomi
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (320.862 KB) | DOI: 10.35791/agrsosek.v19i1.46754

Abstract

This study aims to obtain accurate results from the concentration of old coconut water and BAP on Vacin and Went and Murashige and Skoog media on the growth of Dendrobium sp. through in vitro culture. The method used in this study was a completely randomized design consisting of 8 treatments and 8 replications. Each replication consisted of 2 bottles so that a total of 128 bottles, namely A0 (control, without using old coconut water), A1 (12.5% old coconut water), B0 (control, without using BAP), B1 (BAP 0.5 mg/L ), and used 2 types of media (Murashige and Skoog) and (Vacin and Went). The variables observed included the number of protocorm, wet weight, dry weight, and pH of the media. The results showed that the concentration of old coconut water and BAP had a significant effect on the number of protocorm, fresh weight, and pH of the media on Dendrobium sp. The best treatment obtained was VA1B1 (12.5% old coconut water, 0.5 ppm BAP) with a media pH of 5.0 V A1B1, the number of protocorm V A1B1 49.57, and VA1B1 0.008 g which had the best results compared to other treatments.
Pertumbuhan In Vitro Tiga Varietas Krisan Pada Variasi Media Murashige & Skoog (MS) Kaurow, Tamariska Julita; Mandang, Jeany; Mamarimbing, Rinny
AGRI-SOSIOEKONOMI Vol. 19 No. 1 (2023): Agri-Sosioekonomi
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (163.225 KB) | DOI: 10.35791/agrsosek.v19i1.46756

Abstract

This study aims to determine the growth of three Chrysanthemum varieties on complete MS media, 50% MS and 50% MS which were given coconut water through In vitro culture. This research was conducted from March to May 2022 at the Tissue Culture Laboratory, Faculty of Agriculture, Sam Ratulangi University, Manado. This study used a factorial design in a completely randomized design (CRD) with 2 treatment factors, namely media and varieties consisting of 9 treatments and 4 replications so that a total of 36 bottles, namely MS 100%, MS ½ (MS 50%), MS ½ AK (MS 50% + coconut water 200 ml/l). The variables observed were: plant height, number of leaves, fresh weight, and number of roots. The results of the study showed that the average growth of the 3 Chrysanthemum varieties was better on ½ MS and ½ MS media supplemented with coconut water. The best wet weight on ½ MS+AK media corresponds to the highest number of roots in the same treatment.
Co-Authors Agus Suman Deanne Kojoh Deanne Kojoh, Deanne Edy Lengkong Inkiriwang, Annatje E.B. Kaurow, Tamariska Julita Kawengian, Yefta B Marhaenus J. Rumondor Meklin Bakar Meklin Bakar, Meklin Rinny Mamarimbing Runtunuwu, Semuel saartje Sompotan Soemarno Soemarno Sofia Demasabu Sofia Demasabu, Sofia Sundalangi, Grace Treesye I Turang Wiske Rotinsulu