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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Pharmaceutical Sciences and Research (PSR) JURNAL REKAYASA KIMIA & LINGKUNGAN
Djumali Mangunwidjaja
Departemen Teknologi Industri Pertanian, FTP, Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry Chemical Engineering, Chemistry & Bioengineering Energy Engineering Environmental Science

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Glycerol Utilization as Substrate for Ethanol Production in Escherichia coli Recombinant under an Aerobic Condition Pranata, Wahyu Suradi; Suryadarma, Prayoga; Mangunwidjaja, Djumali
Journal of Tropical Life Science Vol 8, No 1 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1142.696 KB) | DOI: 10.11594/jtls.08.01.14

Abstract

The utilization of glycerol as a substrate for ethanol production in E. coli recombinant harboring ethanologenic gens (PDC and ADHB) under aerobic conditions was investigated. This research was conducted by using E. coli BW25113 which compared the growth characteristics on glycerol and glucose as carbon source. E. coli can grow well on both substrates and it consumed glycerol faster than glucose. On glucose, E. coli occurred overflow metabolism that indicated by high acetate accumulation. Meanwhile, on substrate of glycerol the acetate accumulation could be reduced. Using glycerol substrate on E. coli increased piruvate accumulation, comparing with glucose. Therefore, the growth characteristic of glycerol was more effective. In E. coli ∆pta/pHfdh/pTadhB-pdc utilized glycerol substrate was able to accumulate pyruvate as intermediate metabolite for produce 2.18 gL-1 ethanol.
Potency of (Poly) Acrylic/Carboxymethyl Starch-Chitosan Biohydrogel for Curcumin Oral Delivery Matrix Ruriani, Eka; Mangunwidjaja, Djumali; Richana, Nur; Sunarti, Titi Candra
Pharmaceutical Sciences and Research Vol. 5, No. 1
Publisher : UI Scholars Hub

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Abstract

Biohydrogel has gathered great interest in the pharmaceuticals field. This natural polymers were biodegradable, non-toxic, biocompatible, and its specific ability to response environment change can be considered for the controlled released matric of bioactive compound. In this study, the biohydrogel was synthesized by graft-copolymerization of acrylic acid onto carboxymethyl starch (CMS) and chitosan. The objective of this research was to determine the effect of CMS-chitosan ratio on the biohydrogel characteristic. The acrylic acid was grafted on to the backbone (3:1) using cerric ammonium nitrate as the inisiator.A standarded curcumin was applied to test the binding potency of matrix. A higher CMS ratio in the polymer mixture (4:1) revealed the highest swelling power (16.9 w/w) and percentage of curcumin absorption (17.34%). All samples have pH-responsive swelling properties, with the swelling trend was observed in the order of distilled water >HCl solution > phosphate buffer solution. FTIR spectra and SEM micrographs has confirmed the graft-copolymerization of PAA/CMSCs biohydrogel by describing the appearance of peak around 1600 cm-1and the morphology of granular structure, respectively. The graft-copolymerization of acrylic acid onto the two anionic natural polymer by cerric ammonium nitrate as the initiator has resulted a pH-dependent swelling biohydrogel, and it has the ability to deliver curcumin in stomach-targeted system.
Adsorpsi Surfaktan Nonionik Alkil Poliglikosida pada Antar muka Fluida-Fluida Adisalamun, Adisalamun; Mangunwidjaja, Djumali; Suryani, Ani; Sunarti, Titi Candra; Arkeman, Yandra
Jurnal Rekayasa Kimia & Lingkungan Vol 9, No 1 (2012): Jurnal Rekayasa Kimia & Lingkungan
Publisher : Chemical Engineering Department, Syiah Kuala University, Banda Aceh, Indonesia

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Nonionic surfactants have been used extensively in various industrial applications such as cleaning, personal care, crop protection, paint and coating, textile finishing, emulsion stabilization, food and leather processing. In this study, we have studied the adsorption of three nonionic surfactants of alkyl polyglycoside, namely commercial APG from Cognis and APG produced from glucose as well as APG synthesized from sago starch, at fluidfluid interfaces. The variation of surface and interfacial tension with the concentration of surfactant in the bulk was studied, and the data were fitted using a surface equation of state derived from the Langmuir isotherm. The agreement between ?(c) data and Langmuir isotherm model was very good.Keywords: adsorption, air/water interface, interfacial tension, surface tension
Co-Authors Adisalamun Adisalamun Anas Miftah Fauzi Ani Suryani Bambang Prasetya EKA RURIANI Euis HERMIATI Khaswar Syamsu Lanjar Sumarno Nastiti Siswi Indrasti Nur Richana Ono Suparno Pranata, Wahyu Suradi Sapta Raharja Sunarti, Ttiti C Titi Candra Sunarti Yandra Arkeman