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Respons Morfologis Beberapa Varietas Padi (Oryza sativa L.) terhadap Kekeringan pada Fase Perkecambahan (Morphological Response of Some Rice (Oryza sativa L.) Cultivars to Water Deficit at the Seedling Stage) Ballo, Maria; Nio, Song Ai; Mantiri, Feky R; Pandiangan, Dingse
JURNAL BIOS LOGOS Vol 2, No 2 (2012): JURNAL BIOSLOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.2.2.2012.1045

Abstrak Biji memerlukan sejumlah besar air yang harus diserap sebelum perkecambahan bisa terjadi, yaitu sekitar dua atau tiga kali dari berat keringnya. Penelitian ini menguji konsistensi respons morfologis padi terhadap kekeringan pada fase perkecambahan dengan perlakuan larutan polietilen glikol (PEG) sebagai larutan osmotikum. Penelitian ini dilakukan dengan percobaan faktorial dalam Rancangan Acak Kelompok yang terdiri dari 5 perlakuan PEG 8000 mencakup PEG 0, -0,25, -0,5, -0,75 dan -1,0 MPa terhadap 7 varietas padi, yaitu Beras Merah, IR 64, Burungan, Superwin, Serayu, Aries, dan Cigeulis. Hasil penelitian ini menunjukkan terdapat perbedaan respons morfologis terhadap kekeringan yang diinduksi dengan PEG 8000 pada fase perkecambahan di antara ketujuh varietas padi. Panjang akar seminal, panjang tunas, rasio panjang akar seminal : panjang tunas, persentase perkecambahan dan indeks vigor benih dapat dipakai sebagai indikator toleransi kekeringan yang potensial pada fase perkecambahan padi. Toleransi varietas Superwin terhadap kekeringan yang diinduksi dengan PEG 8000 pada fase perkecambahan lebih tinggi daripada varietas padi lainnya. Kata kunci: indikator, PEG, toleransi, kekeringan Abstract The seeds required a large amount of water, i.e. two or three times of their dry weight, to be absorbed before the germination. This study evaluated the consistency of morphological response of rice to water deficit at the seedling stage. The research was conducted as factorial experiment in Randomized Block Design. The treatments of PEG-8000-induced-water deficit were PEG 0, -0,25, -0,5, -0,75 and -1,0 MPa. Those treatments were applied to 7 rice cultivars, i.e. Beras Merah, IR 64, Burungan, Superwin, Serayu, Aries, and Cigeulis. The results showed the difference of morphological response to PEG-8000-induced-water deficit among 7 rice cultivars at the seedling stage. The seminal root length, shoot length, ratio seminal root length: shoot length, seedling percentage and seed vigor index were able to be used as potential water-deficit-tolerant indicators at the seedling stage. Cultivar Superwin was more tolerant to PEG 8000-induced- water deficit than 6 other rice cultivars. Keywords: PEG, indicator, water- deficit tolerant

Alokasi Biomassa pada Padi (Oryza sativa L.) Lokal Sulut Saat Kekurangan Air (Biomass Partitioning for North Sulawesi Local Rice (Oryza sativa L.) Cultivars under Water Deficit Condition) Nio, Song Ai; Lenak, Audry Agatha; Mantiri, Feky R; Mambu, Susan M; Ludong, Daniel PM
JURNAL BIOS LOGOS Vol 5, No 2 (2015): JURNAL BIOSLOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.5.2.2015.10550

ABSTRAK Salah satu masalah utama yang menjadi kendala dalam produksi padi di Indonesia adalah kekurangan air yang melanda areal persawahan sehingga menjadi suatu ancaman untuk produksi padi. Masalah tersebut dapat diatasi dengan beberapa cara dan salah satunya dapat dilakukan melalui strategi adaptasi. Oleh sebab itu kajian sifat tahan kering pada padi lokal Sulawesi Utara (Sulut) perlu dilakukan, dalam upaya mendukung tercapainya tujuan strategis tersebut. Penelitian ini dilakukan untuk mengevaluasi sifat tahan kering pada empat varietas padi lokal Sulut (Burungan, Superwin, Temo dan Ombong) pada fase vegetatif saat kekurangan air berdasarkan alokasi biomassa. Setelah 14 hari perlakuan, rasio akar:tajuk pada keempat varietas menunjukkan bahwa varietas Superwin, Temo dan Burungan yang tidak diairi air memiliki rasio akar:tajuk yang lebih besar daripada yang diairi. Berdasarkan alokasi biomassa, sifat tahan kering Superwin, Temo dan Ombong lebih besar dibandingkan dengan Burungan, sehingga varietas ini potensial untuk ditanam di daerah kekurangan air. Kata kunci: biomassa, kekeringan, padi lokal Sulut, vegetative ABSTRACT One of the main problems in rice production in Indonesia is the lack of water and this condition resulted in the decrease of rice production. The problem can be solved in several ways and one of them is adaptation strategies. This study was conducted to evaluate drought resistance in four North Sulawesi local rice varieties (Burungan, Superwin, Temo and Ombong) at the vegetative phase under water deficit based on biomass allocation. After 14 days of treatment, the root:shoot ratio of Superwin, Temo and Burungan under drought were greater than under well-watered condition. Based on the biomass allocation, the drought resistance of Superwin, Temo and Ombong were greater than Burungan, so that these varieties were Â potential to be grown in the water deficit areas. Keywords: biomass, drought, North Sulawesi local rice, vegetative

Identifikasi Tumbuhan Paku Air (Azolla sp.) Secara Morfologi dan Molekuler dengan Menggunakan Gen rbcL (Identification of Water Ferns (Azolla sp.) Based on Morphologycal Traits and Molecular Marker Using rbcL Gene) Mantang, Wianita; Mantiri, Feky R.; Kolondam, Beivy J.
JURNAL BIOS LOGOS Vol 8, No 2 (2018): JURNAL BIOSLOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.8.2.2018.21445

AbstrakÂ Azolla merupakan salah satu tumbuhan paku air yang memiliki banyak manfaat, namun belum banyak dikenal dan sering dianggap sebagai tumbuhan gulma. Pengidentifikasian akan keberadaan jenis-jenis tumbuhan paku air (Azolla sp.) penting untuk dilakukan guna mendukung upaya pengembangan, pembudidayaan dan eksplorasi tumbuhan Azolla sp. Penelitian ini bertujuan untuk mengidentifikasi spesies tumbuhan paku air (Azolla sp.) secara morfologi dan molekuler dengan menggunakan gen rbcL. Hasil penelitian menunjukkan bahwa berdasarkan karakter morfologi sampel tumbuhan Azolla sp. asal Tondano Sulawesi Utara menunjukkan kemiripan dengan spesies A. pinnata dan Azolla asal Magelang Jawa Tengah menunjukkan kemiripan dengan spesies A. microphylla. Identifikasi menggunakan sekuens gen rbcL menunjukkan bahwa sekuens sampel tumbuhan Azolla asal Tondano (WM1) memiliki tingkat kemiripan 100% dengan A. pinnata dan Azolla asal Magelang (WM2) memiliki tingkat kemiripan 100% dengan A. microphylla yang terdapat dalam GenBank. Analisis jarak genetik menunjukkan kedua sampel WM1 dan WM2 memiliki hubungan kekerabatan yang cukup dekat dengan nilai jarak genetik 0,060.Kata kunci: Azolla sp., identifikasi morfologi, identifikasi molekuler, gen rbcLÂ AbstractÂ Azolla is one of the water ferns that has many benefits, but it is not yet widely known and is often regarded as a weed plant. Identification of water ferns (Azolla sp.) is important to be carried out to support the development, cultivation, and exploration of Azolla sp. This study aimed to identify species of aquatic plants (Azolla sp.) morphologically and molecularly using the gene rbcL. The results demonstrated that based on the morphological characters, the Azolla sp. from Tondano, North Sulawesi, showed similarity with species A. pinnata and Azolla from Magelang, Central Java, showed similarity to species A. microphylla. Identification using rbcL gene sequences showed that the sample sequence of plants Azolla from Tondano (WM1) had a 100% similarity level with A. pinnata and Azolla from Magelang (WM2) had a 100% similarity level with A. microphylla available in GenBank. Genetic distance analysis showed that both WM1 and WM2 samples had a close relationship with the genetic distance value of 0.060.Key words: Azolla sp., morphological identification, molecular identification, rbcL gene

Kajian ethylene triple response terhadap kecambah tiga varietas kedelai (Study of ethylene triple response on the seedlings of three varieties of soybean) Wardani, Kartika Eka; Mantiri, Feky Reky; Nio, Song Ai; Rumondor, Marhaenus
JURNAL BIOS LOGOS Vol 4, No 2 (2014): JURNAL BIOSLOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.4.2.2014.6152

AbstrakKeterbatasan lahan tanam di Indonesia merupakan salah satu faktor pembatas dalam pembudidayaan tanaman kedelai. Oleh sebab itu kedelai di tumpangsarikan dengan tanaman lain, sehingga ternaungi. Naungan pada tanaman menyebabkan tingginya produksi etilen sehingga tanaman akan memunculkan triple response yang dapat mempengaruhi pertumbuhan dan perkembangan tanaman. Oleh sebab itu, penelitian ini dilakukan untuk menyeleksi varietas kedelai yang tahan dengan adanya peningkatan etilen pada tiga varietas kedelai (Edamamik, Anjasmoro, Wilis). Setelah dikecambahkan selama 5 hari, panjang kedelai varietas Wilis yang diberi etilen (karbid) adalah yang tertinggi dan Anjasmoro adalah yang terendah. Bengkokan kedelai dengan derajat terendah diamati pada varietas Wilis dibandingkan dengan dua varietas lain. Diameter ketiga varietas tidak berbeda antara yang normal dan diperlakukan dengan karbid. Pengamatan ketiga parameter ini menunjukkan varietas Wilis adalah tanaman yang tahan terhadap peningkatan etilen, sehingga varietas ini berpotensi untuk ditanam di naungan.Kata kunci: etilen, triple response, kedelaiAbstractLimitation of arable land in Indonesia is one of the limiting factors in soybean cultivation. Consequently, soybean is sometimes cultivated as an intercropping crop. One of the major problems of intercropped plants is shading. Shading triggers increased production of ethylene, which in turn affects germinating seeds to exhibit ethylene triple response. The study aimed to screen different varieties of soybean (i.e., Edamamik, Anjasmoro, Wilis) for resistance to increased consentrationof ethylene. Results showed that five days after germination, the height of Wilis was the highest, while the height of Anjasmoro was the lowest. Similarly, the degree of hook on Wilis was the lowest compared with the other varieties. Meanwhile, the diameter of the seedlings was not significantly different among the three varieties. Based on these findings it was concluded that Wilis variety was the most resistant to increased concentration of ethylene and therefore was most suited for intercropping (shade environment).Keywords: ethylene, triple response, soybean

Respons Morfologi dan Anatomi Kecambah Kacang Kedelai (Glycine max (L.) Merill) terhadap Intensitas Cahaya yang Berbeda (Morphological and Anatomical Responses of The Soybean (Glycine max (L.) Merill) Sprouts to The Different Light Intensity) Pantilu, Lisa Indried; Mantiri, Feky R; Nio, Song Ai; Pandiangan, Dingse
JURNAL BIOS LOGOS Vol 2, No 2 (2012): JURNAL BIOSLOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.2.2.2012.1044

Abstrak Pengembangan tanaman kedelai sebagai tanaman sela di bawah tegakan karet, hutan tanaman industri (HTI), atau tumpangsari dengan tanaman pangan semusim lain merupakan alternatif andalan untuk meningkatkan produksi kedelai. Penelitian ini bertujuan untuk mengamati respons morfologi dan anatomi kecambah kacang kedelai pada stadium vegetatif 3 terhadap perbedaan intensitas cahaya. Penelitian ini dilakukan dengan menggunakan Rancangan Acak Lengkap (RAL) dengan satu faktor tunggal yaitu Â intensitas cahaya, dengan tiga taraf perlakuan yaitu P0 (tanpa naungan), P1 (naungan paranet 1 lapis untuk naungan Â±50%) dan P2 (naungan paranet 2 lapis untuk naungan Â±90%) dalam tiga kali ulangan. Penelitian ini menggunakan satu varietas kacang kedelai. Morfologi tanaman kedelai pada stadium vegetatif 3 dipengaruhi oleh intensitas cahaya. Hasil uji ANOVA yang dilanjutkan dengan BNT (5%) menunjukkanÂ tinggi tanamanÂ pada P2 dua kali lebih besar dibandingkan dengan tinggi tanaman pada P0; jumlah daun tidak berbeda antara perlakuan P0 dengan P1 dan antara P1 dan P2, tetapi jumlah daun pada P2 lebih banyak dibandingkan dengan jumlah daun pada P0 dan luas daun pada P0 lebih besar dibandingkan dengan luas daun P1 dan P2. Anatomi tanaman kedelai (jumlah, panjang, dan diameter stomata) pada stadium vegetatif 3 tidak dipengaruhi oleh intensitas cahaya. Kata kunci: anatomi, cahaya, kedelai, morfologi, naungan Â Abstract Development of soybean plants as a plant stand waiting at the bottom of the rubber, plantation forests (HTI), or intercropped with other annual scropsis an alternative pledge to increase soybean production. This study aimed to observe the morphological and anatomical responses of soybean sprouts at the vegetative stage 3 of the difference in light intensity. The research was conducted using Completely Randomized Design(CRD) with one single factor is the influence of light, with a three-stage treatment of P0(without shade), P1(1 layer paranet shade to shade Â± 50%) and P2(2 layers for shading paranet auspices ofÂ± 90%) in three replications.This study uses one variety of soybeans. Morphology of soybean plants at the vegetative stage 3 is influenced by light intensity. ANOVA test followed by LSD(5%) plant height at P2 showed two times greater than the height of plants at P0; number of leaves did not differ between treatments P0 with P1 and between P1 and P2, but the number of leaves on P2 more than with the number of leaves on leaf area at P0 and P0 is greater leaf area compared with P1 and P2. Anatomy of soybean plants (number, length and diameter of the stomata) in the vegetative stage 3 is not affected by light intensity. Keywords: anatomy, light, morphology, shade, soybean Â Â

Isolation and Characterization of Potential Lignocellulosic Degrading Bacteria From chicken Manure Compost Zhafira Tiza Maesharoh Soleman; Feky Mantiri; Marhaenus Rumondor
JURNAL BIOS LOGOS Vol. 13 No. 2 (2023): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v13i2.42791

Lignocellulose is the main component that can be found in plants. Lignocellulose consists of three components, namely hemicellulose, lignin, and cellulose. The maximum utilization of lignocellulose cannot be carried out without being degraded, however there are difficulties in carrying out the degradation. The difficulty faced in the degradation process is the presence of lignin components that provide strength and stiffness to the plant, which make it is quite resistant to the degradation process. Lignocellulose degradation requires delignification. The delignification process can be facilitated by enzymes produced by a number of microorganisms, including bacteria can produce lignocellulosic enzymes. This study aims to isolate and characterize lignocellulose-degrading bacteria in chicken manure, and to assess the ability of bacteria to degrade lignocellulose. The results obtained from this research showed that an isolate which has high similarity with Shigella flexneri bacteria which was found in chicken manure and exhibits a potential to degrade lignocellulose.

Isolation and Characterization of Potential Lignocellulosic Degrading Bacteria From chicken Manure Compost Zhafira Tiza Maesharoh Soleman; Feky Mantiri; Marhaenus Rumondor
JURNAL BIOS LOGOS Vol. 13 No. 2 (2023): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v13i2.42791

Lignocellulose is the main component that can be found in plants. Lignocellulose consists of three components, namely hemicellulose, lignin, and cellulose. The maximum utilization of lignocellulose cannot be carried out without being degraded, however there are difficulties in carrying out the degradation. The difficulty faced in the degradation process is the presence of lignin components that provide strength and stiffness to the plant, which make it is quite resistant to the degradation process. Lignocellulose degradation requires delignification. The delignification process can be facilitated by enzymes produced by a number of microorganisms, including bacteria can produce lignocellulosic enzymes. This study aims to isolate and characterize lignocellulose-degrading bacteria in chicken manure, and to assess the ability of bacteria to degrade lignocellulose. The results obtained from this research showed that an isolate which has high similarity with Shigella flexneri bacteria which was found in chicken manure and exhibits a potential to degrade lignocellulose.

Isolation and Identification of Thermophilic Bacteria from Lahendong Hot Spring, North Sulawesi Christiana Abrita Nathania Corneles; Feky Mantiri; Marina Singkoh
JURNAL BIOS LOGOS Vol. 13 No. 2 (2023): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v13i2.50184

Thermophilic bacteria are a group of procaryotic organisms that can grow in high temperature around 450C until 900C. Thermophilic bacteria are able to produce lipase thermostable enzyme. Lipase thermostable enzymes produced from thermophilic bacteria are able to catalyze the hydrolysis of triglycerides into glycerol and free fatty acids and the synthesis of esters in organic solvents. Lipase thermostable enzymes are potential to be used in industrial production. Lipase enzymes have been used for the production of laundry detergents as additives or to replace chemical detergents because of their environmental friendliness. This research was aimed to isolates and identified themophilic bacteria from Lahendong hot springs, North Sulawesi and to conduct the morphological characterization, biochemical test, and molecular identification with 16S rRNA. Results showed that isolates obtained from the- Lahendong hot spring have high similarities with Geobacillus kaustophilus, Bacillus cereus, and Geobacillus lituanicus. The three isolates are able to produce lipase thermostbale enzyme.

Isolation and Identification of Thermophilic Bacteria from Lahendong Hot Spring, North Sulawesi Christiana Abrita Nathania Corneles; Feky Mantiri; Marina Singkoh
JURNAL BIOS LOGOS Vol. 13 No. 2 (2023): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v13i2.50184

Thermophilic bacteria are a group of procaryotic organisms that can grow in high temperature around 450C until 900C. Thermophilic bacteria are able to produce lipase thermostable enzyme. Lipase thermostable enzymes produced from thermophilic bacteria are able to catalyze the hydrolysis of triglycerides into glycerol and free fatty acids and the synthesis of esters in organic solvents. Lipase thermostable enzymes are potential to be used in industrial production. Lipase enzymes have been used for the production of laundry detergents as additives or to replace chemical detergents because of their environmental friendliness. This research was aimed to isolates and identified themophilic bacteria from Lahendong hot springs, North Sulawesi and to conduct the morphological characterization, biochemical test, and molecular identification with 16S rRNA. Results showed that isolates obtained from the- Lahendong hot spring have high similarities with Geobacillus kaustophilus, Bacillus cereus, and Geobacillus lituanicus. The three isolates are able to produce lipase thermostbale enzyme.

Efektivitas Feromon terhadap Interest Kumbang Tanduk (Oryctes rhinoceros) pada Tanaman Kelapa (Cocos nucifera L.) Putri Sri Anggini; Lalu Wahyudi; Feky Recky Mantiri
JURNAL BIOS LOGOS Vol. 12 No. 1 (2022): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v12i1.40116

(Article History: Received Mar 1, 2022; Revised Mar 14, 2022; Accepted Apr 11, 2022)The population of Oryctes rhinoceros that out of control at coconut plantation could be effect on productivity. Therefore, necessary to control using pheromone traps. Pheromones as chemical compounds use to reproduction communicate between individual beetles. The purpose of this study was to reveal the potential of synthetic pheromones in order to control the population of horn beetle (O. rhinoceros). This study conducted observation and response of application pheromone to the population of O. rhinoceros and offcourse the leaf counting as parametre of the degree bettles offensive. The analyze quantitative of sample with associated the decrease coconut productivity. The results of this study indicate that pheromone traps was effective to control the O. rhinoceros by the number of beetles trapped at the area coconut plantations in Pineleng Village, there are160 tails and the ratio of male and female beetles was 1:2. Representative in this area was classified high degree offensive where obtained 6-7 beetles/ha and 45% losses of productivity.Keywords: Oryctes rhinoceros L.; pheromones; coconut plant; attack intensity.Abstrak Populasi kumbang Oryctes rhinoceros yang meningkat secara berkepanjangan tanpa adanya pengendalian dapat menyebabkan penurunan produktivitas kelapa dan kerugian bagi masyarakat, hal ini disebabkan oleh serangan dari kumbang Oryctes rhinoceros. Oleh karena itu diperlukan pengendalian salah satunya menggunakan perangkap berferomon. Tujuan penelitian ini adalah untuk mengungkapkan potensi feromon dalam mengendalikan populasi kumbang tanduk (O. rhinoceros). Penelitian ini menggunakan metode observasi dan respons aplikasi feromon untuk memonitoring populasi kumbang O. rhinoceros dan mengetahui intensitas serangan dengan cara menghitung pelepah daun kelapa yang terserang selama dua bulan disetiap minggunya. Kemudian dianalisis secara kuantitatif sederhana dan dihubungkan dengan penurunan produktivitas kelapa. Hasil penelitian menunjukan bahwa penggunaan perangkap berferomon sebagai pengendalian kumbang O. rhinoceros sangat efektif dengan jumlah kumbang yang terperangkap di perkebunan kelapa dalam Desa Pineleng sebanyak 160 ekor dengan perbandingan kumbang jantan dan betina yaitu 1:2. Intensitas serangan kumbang tergolong dalam kategori tinggi mencapai 6-7 ekor/ha dan menyebabkan penurunan produktivitas buah sebesar 45 %.Kata kunci: Oryctes rhinoceros L.; feromon; tanaman kelapa; intensitas serangan.

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