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All Journal HAYATI Journal of Biosciences Media Veteriner Jurnal Ilmu Dasar Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Jurnal Ilmu Ternak Veteriner JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) BERITA BIOLOGI Proceeding INTERNATIONAL SEMINAR IMPROVING TROPICAL ANIMAL PRODUCTION FOR FOOD SECURITY Biosaintifika: Journal of Biology & Biology Education Makara Journal of Science
ENDANG TRI MARGAWATI
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Control & Systems Engineering Earth & Planetary Sciences Mathematics Veterinary Other

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Journal : Media Veteriner

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Maturing Singly Versus in Groups on Ovine Oocyte Meiosis Endang Tri Margawati
Media Veteriner Vol. 5 No. 3 (1998): Media Veteriner
Publisher : Media Veteriner

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Abstract

The effect of maturing systems (single vs groups) of ovine oocytes was studied to examine the oocyte development in vitro. Oocytes were aspirated from follicle ovaries collected at local abattoirs Cibinong West Java, using a 18-G needle. Aspiration medium consisted of H 199 + 2 % FCS + 50 μg/ml Heparin and the maturating medium (IVM) consisted of B 199 - 10 % FCS + 10 μg/ml FSH + 10 μg/ml hCG + 1 μg/ml Estradiol. The oocytes collected were divided into three groups and treated separately as follows: T1) oocytes were matured singly in 50 μl IVM medium. T2) every five oocytes was matured in 50 μl IVM medium and T3) every 10 oocytes was matured in 50 μl IVM medium. All oocytes were maintained in incubator with 5 % CO, and high humidity at 38 °C for 20 h. The resulting ova were stained in 1 % lacmoid then examined for meiosis division under a microscope. There was a signiticant effect among treatments on the proportion oocytes reaching metaphase II (P
Co-Authors Chalid Thalib Daud Samsudewa HARIMURTI MARTOJO Heni Utami Ningsih, Heni Utami HERMAN WILLEM RAADSMA Indriawati Indriawati Indriawati, Indriawati, Indriawati Indriawati, Indriawati Indriawati, Indriawati Jakaria Jakaria Martien Herna Susanti Muhamad Ridwan Muhammad Ridwan Pintaka Bayu Putra, Widya Reza, Muhammad Aulia Ronny Rachman Noor Salfia, Mega Slamet Diah Volkandari, Slamet Diah SUBANDRIYO SUBANDRIYO Tatag Bagus Putra Praakarsa, Tatag Bagus Putra