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Arief Mulyono
Balai Besar Penelitian dan Pengembangan Vektor dan Reservoir Penyakit Salatiga
Health Professions Immunology & microbiology Public Health Veterinary

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LEPTOSPIROSIS CASE FINDING FOR DEVELOPMENT OF LEPTOSPIROSIS SURVEILLANCE IN SEMARANG CITY, CENTRAL JAVA, INDONESIA Ristiyanto Ristiyanto; Farida Dwi Handayani; Arief Mulyono; Arum Sih Joharina; Tri Wibawa; Setyawan Budiharta; Supargiono Supargiono
Vektora : Jurnal Vektor dan Reservoir Penyakit Vol 10 No 2 (2018): Vektora : Jurnal vektor dan reservoir penyakit
Publisher : Balai Besar Penelitian dan Pengembangan Vektor dan Reservoir Penyakit (B2P2VRP) Salatiga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (453.719 KB) | DOI: 10.22435/vk.v10i2.284

Abstract

Leptospirosis is primarily a disease of wild and domestic animals, and may be transmitted to humans through direct or indirect contact with infected animal urine. Leptospirosis is endemic in part of Indonesia territory. This work aimed to find the leptospirosis cases in Semarang city, one of the endemic leptospirosis in Indonesia by active and passive case finding. The leptospirosis were screened in the community base active case finding and passive case finding in primary health care center and hospitals, by using modified WHO-SEARO criteria and laboratory confirmation test. There were 191 cases detected with modified WHO-SEARO criteria, and among those cases only 31 cases confirmed by laboratory test, either by using Rapid Detection Test (RDT), Microscopic Agglutinations Test (MAT) or Polymerase chained reaction (PCR). It was found that leptospirosis was not correlated with rainfall season. Fever, headache and myalgia were the most common symptoms experienced by >90% patients. Active case finding add no more than 7% probable leptospirosis and 14% confirmed cases to the traditional passive case finding. It was conclude that leptospirosis case finding is still challenging in the endemic area, such as Semarang city. The application of modified WHO-SEARO criteria as diagnostic tool may be needed to be further evaluated. Keywords: Leptospirosis, Surveillance, Case finding, Endemic, Detection
DETEKSI VIRUS HEPATITIS E (HEV) DAN HANTAVIRUS PADA INANG RESERVOIR (TIKUS) DI KABUPATEN KLATEN DAN KENDAL, PROVINSI JAWA TENGAH Arief Mulyono; Tika Fiona Sari; Ristiyanto Ristiyanto; Bernadus Yuliadi; Edi Royandi; Ayu Pradipta Pratiwi
Vektora : Jurnal Vektor dan Reservoir Penyakit Vol 11 No 2 (2019): Vektora : Jurnal Vektor dan Reservoir Penyakit
Publisher : Balai Besar Penelitian dan Pengembangan Vektor dan Reservoir Penyakit (B2P2VRP) Salatiga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (449.615 KB) | DOI: 10.22435/vk.v11i2.2104

Abstract

Rats are animal reservoirs and harbours of several zoonotic pathogens diseases in humans. At least, there are 68 viruses of zoonotic agents that can be transmitted by rats. Two common types of viruses attackinghumans are Hepatitis E virus (HEV) and Hantavirus. Early detection of those viruses is fundamentally required in order to prevent disease transmissions to humans. The aim of the study was to detect and count the percentage of rats infected by HEV and Hantavirus in Kendal and Klaten Districts, Central Java Province. The research design used in this study was descriptive design with cross-sectional approach. The target population was rats distributed in Klaten and Kendal Districts. In addition, the research subject was trapped rats. Detection of Hantavirus was carried out using ELISA method and detection of HEV was conducted using nested reverse transcription PCR (nested RT-PCR). A total of 73 rats was successfully captured consisting of 2 genus and 4 species e.g. Rattus novergicus, R. tanezumi, R. tiomanicus and Bandicota indica. The trapped rats infected by HEV were 3.7% and 41.3% for Klaten and Kendal, respectively. However, the seropositive of Hantavirus was only found in Kendal District (20.5%). Rat control is necessary to prevent transmission of HEV and Hantavirus.
Co-Authors Aprilia Safitri Arum Sih Joharina Ayu Pradipta Pratiwi Bernadus Yuliadi Bernadus Yuliadi Diana Andriyani Pratamawati Edi Royandi Farida Dwi Handayani Muhidin Muhidin Nur Hidayati Ristiyanto Ristiyanto Ristiyanto Ristiyanto Ristiyanto Ristiyanto Setyawan Budiharta Supargiono Supargiono Tika Fiona Sari Tri Wibawa Wening Widjajanti