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Perilaku Masyarakat Dan Indeks Entomologi Vektor Demam Berdarah Dengue Di Kota Cimahi Firda Yanuar Pradani; M. Ezza Azmi Fuadiyah; yuneu yuliasih
ASPIRATOR - Journal of Vector-borne Disease Studies Vol 2 No 1 (2010): Jurnal Aspirator Volume 2 Nomor 1 2010
Publisher : Loka Litbang Kesehatan Pangandaran

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Dengue haemorhagic fever (DHF) was one of most dangerous disease in Indonesia. The number of case showed increased year by year since first time observed. Vector control that used by government always using Insecticides. A continuity of it cancaused resistances of mosquito, and it will be more dangerous.In District Cimahi, vector control doing by mosquitoes habitat eradication (PSN) for last 3 years. It done by invite people to participate to make environment clean. Every week, peopleon duty going house to house to check water container condition. They also give support and suggest how to prevent breeding mosquitoes to people. The aim of this research is to evaluate knowledge, practice and attitude people about DBD and to count Entomology Index in district Cimahi. This research observe that globally people attitude is good, knowledge is middle and practicestill bad. Larvae Free Index (Angka Bebas Jentik) when observe show that in Cibabat first week still below national Larvae Free Index. In Pasirkaliki, first week and second week Larvae Free Index below national Larvae Free Index.
Mortalitas Aedes albopictus akibat infeksi horizontal Beauveria bassiana dan aktivitas enzim Kitinase B. bassiana Firda Yanuar Pradani; Mutiara Widawati
ASPIRATOR - Journal of Vector-borne Disease Studies Vol 7 No 2 (2015): Jurnal Aspirator Volume 7 Nomor 2 2015
Publisher : Loka Litbang Kesehatan Pangandaran

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Abstrak. Demam berdarah dengue (DBD) merupakan penyakit yang disebabkan oleh virus dengue dan ditularkan melalui gigitan nyamuk Aedes aegypti sebagai vektor primer dan Ae. albopictus sebagai vektor sekunder. Pengendalian vektor selama ini dilakukan dengan menggunakan insektisida dengan resiko terjadi resistensi. Upaya pencarian insektisida alternatif yang ramah lingkungan dan aman terus dilakukan dengan melakukan penelitian potensi mikroorganisme seperti bakteri dan fungi. Salah satu fungi yang diketahui memiliki daya patogen terhadap nyamuk adalah B. bassiana. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian konidium B. bassiana dengan konsentrasi berbeda terhadap mortalitas Ae. albopictus. Penelitian ini juga bertujuan untuk melihat pengaruh kondisi nyamuk terhadap infeksi fungi, potensi infeksi horizontal dari nyamuk jantan yang terinfeksi kepada nyamuk betina sehat dan aktivitas enzim kitinase yang dihasilkan oleh B. bassiana. Data kematian dianalisis menggunakan uji univariate dan dilakukan uji lanjut dengan menggunakan uji Tukey. Hasil penelitian menunjukkan bahwa konsentrasi konidia berpengaruh terhadap mortalitas Ae. albopictus. Kondisi nyamuk tidak ikut mempengaruhi mortalitas tetapi mempengaruhi kerentanan nyamuk. Tidak ada interaksi dari kedua faktor yang diujikan sehingga kedua faktor berdiri sendiri dalam menentukan kematian nyamuk. Pada konsentrasi 105 angka kematian nyamuk adalah sebesar 23,05%. Konsentrasi lebih tinggi yaitu 107 mengakibatkan mortalitas yang lebih tinggi yaitu sebesar 31,47%. Indeks kitinolitik B. bassiana adalah sebesar 1,67 dan aktivitas spesifik enzim adalah sebesar 1,0557 unit/mg.
Resistance Detection of Aedes aegypti Larvae To Cypermethrin from Endemic Area In Cimahi City West Java Endang Puji Astuti; Mara Ipa; Firda Yanuar Pradani
ASPIRATOR - Journal of Vector-borne Disease Studies Vol 6 No 1 (2014): Jurnal Aspirator Volume 6 Nomor 1 2014
Publisher : Loka Litbang Kesehatan Pangandaran

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Abstract. Vector control programs using chemical insecticide e.g organochlorin, organophosphate, carbamate, and pyrethroid (cypermethrin). When those insecticides were applied continuously, it may lead to vector resistance. The aim of this research was to detect any resistance of Ae. aegypti to cypermethrin in endemic areas of Cimahi. This research is a laboratory study that used biochemical test which referred to Lee’s method. Larva samples were collected from 8 villages, which are endemic area. Samples of larvae were collected from 15 villages belonged to dengue endemic areas in town of Cimahi, however, villages that meet the availability of larvae were only 8 villages. To detect the activity of monooxygenase enzyme, a biochemical assay was used in this research by created a reaction between larvae homogenate and sodium acetate substrate. The results of reaction were read using ELISA reader with spectrophotometer wave length of 595 nm. Overall, the results showed that most of the larvae in eight villages of Cimahi is still susceptible to cypermethrin. However, larvae from Cibabat village were 4% resistant, 2% tolerant, and 94% susceptible. On the other hand, Cigugur village showed that 12.7% larvae were tolerant and 87.3% still susceptible. Other villages like Cimahi, Cibeureum, Melong, Baros, Cipageran, and Pasirkaliki still remains susceptible. Resistance detection using biochemical assay of cypermethrin insecticide for Ae.aegypti resulting data stated that in 6 villages were still susceptible but in 3 other villages were already tolerant and 1 village was already resistance.
Status Resistensi Aedes aegypti dengan Metode Susceptibility di Kota Cimahi terhadap Cypermethrin Firda Yanuar Pradani; Mara Ipa; Rina marina; Yuneu Yuliasih
ASPIRATOR - Journal of Vector-borne Disease Studies Vol 3 No 1 (2011): Jurnal Aspirator Volume 3 Nomor 1 2011
Publisher : Loka Litbang Kesehatan Pangandaran

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Abstracts: Vector control of dengue usually doing by using insecticides, whether by governmentor insecticides used in household. Using to much insecticides for long time can causedresistence of mosquito. This research aim to know resistance status of Aedes aegypti fromendemic rural in district Cimahi to cypermethrin (synthetic pyretroid). Resistance statusknowing by susceptibility methods (WHO standard) with using impregnated paper that containingcypermethrin 0.2% and 0.4%. Aedes aegypti spread by it for 15, 30, 45 and 60minutes. Number of mortality count by percentage death mosquito in each time and eachconcentration of cypermethrin. Data interpreted by WHO standard, which percentage ofdeath mosquito <80% is resistance, between 80-98% is tolerance and 99-100% is susceptible.Aedes aegypti from endemic rural in district Cimahi showed resistance of cypermethrin0.2% and 0.4%. This result showed that all mosquito still alive after 15 minutes spreadingby cypermethrin 0.2%, and only 6.7% mosquito death in cypermethrin 0.4%. After 30minutes, death mosquito counted 46.7% in cypermethrin 0.2% and 73.3% in 0.4%. 46.7%mosquito was death in 45 and 60 minutes spreading by cypermethrin 0.2% and 73.3% in0.4%. The result showed resistance ratio (RR50) of mosquito is 4.6. Aedes aegypti from endemicrural in district Cimahi showed a resistance to cypermethrin 0.2% and 0.4%.
Indeks Pertumbuhan Larva Aedes aegypti L. Yang Terdedah Dalam Ekstrak Air Kulit Jengkol (Pithecellobium lobatum) Firda Yanuar Pradani
ASPIRATOR - Journal of Vector-borne Disease Studies Vol 1 No 2 (2009): Jurnal Aspirator Volume 1 Nomor 2 2009
Publisher : Loka Litbang Kesehatan Pangandaran

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Abstract

Abstracts. The control of dengue fever (DBD) vector generally use the synthetic insecticides,however it’s utilization had negative effect to the environment. Jengkol(Pithecellobium lobatum) was one of the plants which could be used as larvacidealternative because it had phenolate acid, alkaloid, terpenoid and saponin in its rind. Thisresearch was conducted to know the growth index of Aedes aegypti larvae which wassoaked in the extract of P. lobatum rind. This experiment used P. lobatum rind ekstract atconcentration 36%, 18%, 9% and 0% . The data were analyzed by using Zhang et.almethode (1993). The P. lobatum rind extract were toxic to larvae especially in first andsecond instar at concentration 17,94% respectively. Relativelly growth index range from0,817-1, it is mean that some larvae were stuned, and some were growth into the nextphase.
Co-Authors Endang Puji Astuti M. Ezza Azmi Fuadiyah Mara Ipa Mutiara Widawati Rina Marina Yuneu Yuliasih