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All Journal Jurnal Kesehatan Masyarakat Dental Journal (Majalah Kedokteran Gigi) Jurnal Kedokteran Gigi Universitas Padjadjaran Conservative Dentistry Journal

Widya Saraswati

Department Of Conservative Dentistry, Faculty Of Dental Medicine, Universitas Airlangga

Author-ID : 3196156

Dentistry Health Professions Public Health

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Konsentrasi Bunuh Minimal (KBM) Ekstrak Propolis Lawang terhadap Candida albicans Alvita Wibowo; Ira Widjiastuti; Widya Saraswati
Conservative Dentistry Journal Vol. 7 No. 1 (2017): January - June
Publisher : Universitas Airlangga

Background: Candida albicans is the most resistant fungi in root canals. The existence of Candida albicans after root canal treatment can cause the failure of root canal treatment. The prevalence of this case is 36,7%. Propolis is a resinous substance which has many pharmaceutical and biological effects such as antimicrobial activities. Its active components include flavonoid, saponin, and tannin, have an important role in antimicrobial activities. In this study, antifungal aspect of propolis extract was investigated. Purpose: The aim of this study was to determine the minimum fungicidal concentration (MFC) of propolis extract againts Candida albicans. Methods: This research was a laboratory experimental study. Propolis extract produced by propolis farm from Lawang. The antifungal activity test was performed by colony counting fungi in sabouraud’s dextrose agar (SDA) to determine the value of MFC of propolis extract againts Candida albicans. Result: At the concentration of 72,5%, 73,5%, 74,5%, 75,5%, 76,5%, and 77,5% there are decreases in the number of Candida albicans fungal colonies when compared with positive control group. There are significant differences in each study group (p<0,05). At the concentraion of 76,5% was not revealed any fungal growth of Candida albicans, it was because the antifungal effect of propolis extract has reached MFC. Conclusion: The minimum fungicidal concentration of propolis extract againts Candida albicans is 76,5%.

Kemampuan hambat ekstrak temulawak (Curcuma xanthorrhiza Roxb.) terhadap adhesi bakteri Streptococcus mutans Erin Imaniar B; Sri Kunarti; Widya Saraswati
Conservative Dentistry Journal Vol. 7 No. 1 (2017): January - June
Publisher : Universitas Airlangga

Background : Streptococcus mutans is the most cariogenic microorganism in the oral cavity. Streptococcus mutans has the ability to use dietary carbohydrates, such as sucrose, to synthesize extracellular polysaccharides (glucan and fructan) through glucosyltransferase and fructosyltransferase. Extracellular polysaccharide has a role in promoting the adhesion of bacteria. Chlorhexidine is the gold standard as antiplaque agent but chlorhexidine is not fully able to inhibit the adhesion of bacteria because it has an effect that can increase gtfC and gtfD expression on S. mutans in planktonic form. Temulawak extract (Curcuma xanthorrhiza Roxb.) contains of curcumin, xanthorrhizol, saponin, flavonoid and tannin that have an effect to inhibit activity and secretion of glucosyltransferase and fructosyltransferase. Purpose: The aim of this study is to find out the ability of temulawak extract (Curcuma xanthorrhiza Roxb.) to inhibit adhesion of Streptococcus mutans. Method: This study was designed as an experimental laboratory study with post test only control group design using Streptococcus mutans. Temulawak was extracted using maceration method. The number of bacteria that can perform adhesion to the tooth surface can be determined by performing calculations using formulas plate count method. Result: Temulawak extract (Curcuma xanthorrhiza Roxb.) at concentrations of 25%, 37.5%, 50% and chlorhexidine can inhibit bacterial adhesion of Streptococcus mutans. Conclusion: Temulawak extract at concentration of 50% and 37.5% has the best ability to inhibit the adhesion of bacteria among other treatment groups.

Kekuatan Perlekatan Geser Tumpatan Semen Ionomer Kaca pada Dentin setelah Aplikasi Dentin Conditioner dan Cavity Conditioner Dewi Kusuma Wardani; Ruslan Effendy; Widya Saraswati
Conservative Dentistry Journal Vol. 8 No. 1 (2018): January - June
Publisher : Universitas Airlangga

Backround: Smear layer formed during cavity preparation interfere with the adhesion between restoration and tooth structure and is considered a barrier that would degrade the quality of adhesion. Smear layer does not have a stable substrate for adhesion, gradually layer dissolves in the restorative material and cause microleakage, penetration of bacteria and inflammation of the pulp. Adhesion to dentin is more difficult to achieve than enamel, therefore the cleaning procedure on dentin require special treatment. Conditioner form a weak acid is used to remove the smear layer and surface contamination on the email or dentin which can reduce the adhesion of the material and the tooth surface. Dentin conditioner is an acid material containing 10% polyacrylic acid conditioner while Cavity conditioner an acid material containing 20% polyacrylic acid and 3% aluminum chloride. The higher the concentration, the more smear layer is dissolved in order to obtain adhesion of glass ionomer cements better because it is not blocked by the smear layer. Purpose: The purpose of this laboratory research is to study the shear bond strength of glass ionomer cement in dentin after application dentin conditioner and cavity conditioner .Material and Method: Twenty seven bovine cow's teeth were divided into three groups. Each group consisted of nine samples. Group 1 was control (without conditioner). Group 2 was treated with the Dentin conditioner. Group 3 was treated with the Cavity conditioner. Result: The average shear bond strength in group 1 is 3.31 Mpa, group 2 is 7.74 MPa and group 3 is 9.92 Mpa. Conclusion: There is a significant difference between third group and the shear bond strength of glass ionomer cement on dentin with application of the Cavity conditioner is higher than with application of the Dentin conditioner and without application conditioner

The Difference of The Effects of Conventional Flowable Composite and Self-Adhering Flowable Composite on BHK-21 Fibroblast Cells Anuj Bhardwaj; S. Sukaton; Anggi Puspitasari; Widya Saraswati
Conservative Dentistry Journal Vol. 8 No. 2 (2018): July - December
Publisher : Universitas Airlangga

Background: One type of composite resin material on the market is flowable composites (FC) which has low viscosity, can be applied to areas with low stress or require good penetration such as pit and fissure sealants, restoration of class II, class III, and class V. Along with technology development, self-adhering flowable composite (SAFC) material has been developed which shorten the applications time because it combines etch, priming, and bonding in one system. The incomplete composite polymerization process can release residual monomers which affect pulp and gingiva The effects of composite materials can be seen from the viability of BHK-21 fibroblast cells after being exposed by these materials. Aims: Determine the viability of BHK-21 cells after being exposed to conventional flowable composite (CFC) and SAFC. Method: The research was in-vitro experimental laboratory with post-test only control group design. BHK-21 cell cultures were included in a 96-well microplate and divided into control group (N=16) and two treated groups (N=16). The treated group was given CFC and SAFC in a disk form with 5mm in diameter and 2,5mm in thickness, then incubated for 24 hours. MTT was given, the optical density value was read by ELISA reader and cell viability was calculated. Optical density data were analyzed using Tukey HSD to compare between groups. Results: The BHK-21 cells viabitlity of SAFC group is greater than the CFC, , indicated by the optical density SAFC (value=0.1233) and CFC (value=0.0936). Conclusion: The viability of BHK-21 cells exposed to SAFC is higher than that of CFC.

TENSILE STRENGTH OF BONDING MATERIAL TO DENTINE USING WATER-WET AND ETHANOL-WET TECHNIQUES Ainin Nafilatus; M. Mudjiono; Widya Saraswati
Conservative Dentistry Journal Vol. 8 No. 1 (2018): January - June
Publisher : Universitas Airlangga

Background: Composite resins have hydrophobic properties so it cannot attach to dentine. Moist dentin is a normal condition caused by the presence of fluid in dentinal tubules. Therefore bonding material is required to bond dentine with composite. Water-wet technique is a technique in which dentine surface is rinsed after etching by using water and allowing it to be moist and prevent the collapse of dentine collagen fibrils. This technique is commonly used today, but excess water disturbs the effectiveness of dentine and bonding material attachment. Ethanol-wet technique is a technique in which ethanol is used to replace water before bonding to reduce excess water in dentine surface. This technique will initiate monomer penetration into interfibrillar space of dentine. It will affect the result of tensile strength test. Purpose: The purpose of this study was to compare tensile strength of bonding material to dentine using water-wet and ethanol-wet techniques. Method: 12 samples of bovine incisors teeth were randomly divided into 2 group. Each group consists of six samples. Group 1 was treated with water-wet technique and group 2 was treated with ethanol-wet technique. Autograph was used as a tensile strength test instrument. Result : The average of tensile strength using water-wet technique was 2,16067 MPa and ethanol-wet technique was 4,21900 MPa. Conclusion: Ethanol-wet technique have higher value of tensile strength than water-wet technique.

ANTIBACTERICAL POTENCY OF CALCIUM HYDROXIDE AND PROPOLIS MIXTURE ON MIXED BACTERIA OF CARIOUS DENTIN Ruslan Effendy; Vita Ariesta; Widya Saraswati
Conservative Dentistry Journal Vol. 9 No. 1 (2019): January - June
Publisher : Universitas Airlangga

Background: Calcium hydroxide has been known as the gold standard of pulp capping process, but still has some weaknesses such as a pH that is too high to be potentially toxic, resistant to certain bacteria, causing necrosis of the pulp, and causing a tunnel defect. This weakness causes researchers to look for alternative ingredients that have antibacterical properties that can be combined with calcium hydroxide. Propolis has antibacterial properties such as flavonoids, tannins, saponins, and terpenoids which can work as the best carrier that allows dissociation of ions from calcium hydroxide so the combination of both that has antibacterial power is expected to have an effective antibacterial potency against the number of bacterial colonization with mixed dentine carious. Aims: This study aims to determine the antibacterial potency produced by a combination of calcium hydroxide and propolis against mixed bacteria of carious dentine. Methods: The research was carried out using 4 treatment groups with each of 7 samples. Group I is a combination of calcium hydroxide and propolis with a ratio of 1: 1, group 2 with a ratio of 1: 1.5, group 3 with a ratio of 1: 2, and a positive control group. Each sample was put into a suspense of mixed bacteria of carious dentine, grown for 24 hours. Suspense is vortexed for 1 minute so the solution becomes homogeneous. Put 0.1 ml of suspended mixed bacteria of dentine carious from BHIB media, grown for 24 hours on MHA media and counted the number of colonies. Results: The highest number of colonies in the positive control group, then group 1, group 2, and the least number of colonies in group 3 with the most extract of propolis. Conclusion: The combination of calcium hydroxide and propolis with a ratio of 1: 2 has the best antibacterial power in inhibiting colonization of mixed bacteria of carious dentine.

Effect of 405 nm Diode Laser with Varying Irradiation Time on BHK-21 Fibroblast Viability Kun Ismiyatin; Leidy Herlin Rumbiak; Widya Saraswati; Sri Kunarti; Anuj Bhardwaj
Conservative Dentistry Journal Vol. 9 No. 1 (2019): January - June
Publisher : Universitas Airlangga

Background: Laser is a device that emits light through a process of optical amplification based on the stimulated emission of electromagnetic radiation; it can be used for surgery, detoxification, bio stimulation and antibacterial. However, lasers have Biphasic Dose Response (BDR), which is bio stimulation and bio inhibition. To determine if 405 nm laser diode is biocompatible, viability test is necessary before these lasers can be labeled as viable to use in dental therapy. Aim: To prove the variation of radiation time of the 405 nm laser diode radiation can cause bio stimulation and bio inhibition response that affects the viability of BHK-21 fibroblast cells. Method: Viability test was carried out using BHK-21 fibroblast cells which were inserted into 96-well microplate, then radiated with 405 nm laser diode with varying irradiation time of 30s, 60s, 120s, 240s and 480s. After radiation, the cells are then incubated for 24h. Cytotoxicity was observed using MTT assay and ELISA reader. Data was analyzed using the Kolmogorov-Smirnov test, Levene Test, Welch ANOVA, and Tukey HSD. Results: BHK-21 fibroblast cells radiated with 405 nm laser diode with radiation time of 30s, 60s, 120s, and 240s have the same viability as the control cell, while at 480 seconds the viability exceeds that of the control cell. Conclusion: 405 nm laser diode with radiation times of 30s, 60s, 120s, and 240s do not affect the viability of BHK-21 fibroblast cells. Meanwhile, 480s irradiation time of 405 nm laser diode causes bio stimulation response that increases the viability of BHK-21 fibroblast cells.

Viability Test of Photodynamic Therapy with Diode Laser Waves Length 405 nm on BHK-21 Fibroblast Cells with Various Irradiation Distances Sri Kunarti; Vina Zavira Nizar; Widya Saraswati
Conservative Dentistry Journal Vol. 9 No. 2 (2019): July - December
Publisher : Universitas Airlangga

Background: Photodynamic therapy has now become popular, but its cytotoxic effect is still unclear. In order to be considered suitable for oral cavity therapy, the therapy must not be toxic or cause adverse effects on the target tissue. Viability testing for photodynamic therapy is important to do. Fibroblast cells are often used for testing the toxicity of dentistry because they are the most important cells in the components of the pulp, periodontal ligament, and gingiva. Purpose: To prove the effect of irradiation distance on photodynamic therapy on the viability of BHK-21 fibroblast cells. Method: Viability test was performed with BHK-21 fibroblast cells placed on a 96 well microplate which was then irradiated with 405 nm photodynamic therapy with varying irradiation distances of 1, 4, 7, 10, 13, and 16 mm. After irradiation, cell viability was tested by MTT assay and ELISA Reader. Data were analyzed using Kolmogorov-Smirnov, Levene’s test, Kruskall Wallis, and Tukey HSD. Result: Fibroblast cells with 4 mm irradiation distance have viability over control cells, whereas at irradiation distances 1, 7, 10, 13, and 16 mm have less viability than control cells. Conclusion: Photodynamic therapy 405 nm with 4 mm irradiation distance gives a biostimulation response so that the viability of BHK-21 fibroblast cells increases.

The Effect Of Self-Etch And Total-Etch Bonding Systems Application On Microleakage Of Bulkfill Flowable Composite Restoration In Carbonated Drink Immersion Widya Saraswati; Auryn Thania Song Hadinata; Sukaton Sukaton
Conservative Dentistry Journal Vol. 9 No. 2 (2019): July - December
Publisher : Universitas Airlangga

Background: Resin composite is more advanced today, but the marginal leakage still can not be avoided, for it takes the bonding system. Bonding system aims to provide a layer between a substrate and composite so it has a strong bond . The current latest bonding system is called universal bonding. It can be used with 2 techniques, both self etch and total etch. Universal bonding contains 10MDP so there’s no need for etching and priming. 10MDP results in better adhesion than previous bonding monomers generation. A total etch technique require a pre-etching step to remove smear layer so that it formed porous as a retentive sites. Purpose : Distinguish the marginal leakage of flowablebulkfill composite overlays with the application of total etch and self etch bonding systems accompanied by immersion of carbonated beverages . Method: Tooth samples were prepared to a depth of 2mm, then the bonding agent was applied directly to the cavity without etching (self etch), etched before the application of bonding agent (total etch ), directly loaded without pre-etching and bonding (control) . using a flowablebulkfill composite as a material restorative. All of the sample both total etch and self-etch soaked in Cola drinks for 5 minutes then repeated until 10 treatment each day (one day is assumed to be 12 hours) up to 7 days, followed by immersion in the methylene blue 0.5 % for 24 hours . Sample then splittedbuccolingual direction, leakage was assessed by scoring the edge trough the depth of methylene blue penetration. Result: Mann-Whitney Test showed a significant differences (P>0.05 ) each group . Conclusion: Marginal leakage of composite restoration with total etch technique and self etch technique is smaller than the control group, and the marginal leakage of composite restoration with total etch technique is smaller than that of self etch group .

The Duration Effectivity of Diode Laser 405 nm with Erythrosine Photosensitizer in Killing Streptococcus Mutans Sri Kunarti; Anin Dita; Widya Saraswati
Conservative Dentistry Journal Vol. 10 No. 1 (2020): January-June
Publisher : Universitas Airlangga

Introduction: Dental caries is a multifactorial disease caused by the interaction of pathogenic bacteria, especially Streptococcus mutans. Various caries prevention chemically and mechanically can help reduce the number of bacteria. However, this approach does not cover the entire bacterial population with a photodynamic therapy approach with the addition of photosensitizers and low-intensity light radiation with the right wavelength able to eliminate the number of S. mutans bacteria. Purpose:To determinethe duration of effective irradiation time on a 405 nm diode laser with erythrosine photosensitizer in killing S. mutans bacteria.Methods: This research was an experimental laboratory type with 25 S. mutans samples divided into 5 groups treated with erythrosine photosensitizer 0.1 mg / ml and 405 nm diode laser irradiation with 45 seconds, 60 seconds, 75 seconds duration and no erythrosine photosensitizers and without irradiation. The growth of S. mutans bacteria was calculated then the data were analyzed statistically.Results:the research found that S. mutans bacteria decreased gradually in each group; erythrosine without photosensitizer and without irradiation had the highest average growth rate of 71.6 CFU / ml; without photosensitizer with irradiation of 40.6 CFU / ml; irradiation with photosensitizer 45 seconds at 20.6 CFU / ml; irradiation with 60 seconds photosensitizer at 11 CFU / ml; and irradiation with 75 seconds photosensitizer at 0 CFU / ml. In statistical tests, the data are normally distributed and homogeneous. There are significant differences between groups.Conclusion: The effective duration in this study was found at 75 seconds.

Co-Authors Adioro Soetojo Agnes Robia Ambarioni Ainin Nafilatus Alfina Putri Nurrahmania Alvita Wibowo Ambarwati, Venny Lusanda Anggi Puspitasari Anin Dita Annisa Rahma Annisa Salsabila Andika Putri Anuj Bhardwaj Arnesti Wahab, Sofi Arshila, Dea Auryn Thania Song Hadinata Avicenna, Cathelea Raihan Cathelea Raihan Avicenna Cecilia G.J.Lunardhi Chaerun Mutmainnah Cinitra Anindya Daradjat, Kinanti Naianuha Devi Eka Juniarti Dewi Kusuma Wardani Dian Agustin Wahjuningrum Dian Pramita Ayu Kumalasari Dur Muhammad Lashari Dyah Utari Wahyu Ningrum Dzulfikri, Ahzahra Indah Ekawati Ekawati Eric Priyo Prasetyo Erin Imaniar B Evelyn Tjendronegoro Febriastuti Cahyani Gabriela Kevina Alifen Galih Sampoerno Ganiezha Cindananti Ghozali Pangestu, Dede Goenharto, Setyabudi Gusniarti, Haninda Dhipta Halima, Siti Noorraida Ida Ayu Putu Sri Widnyani Ira Widjiastuti Jaelani, Nurshiffa Hanifatul Amrina Karlina Samadi Ketut Suardita, Ketut Kun Ismiyatin Latief Mooduto, Latief Leidy Herlin Rumbiak Lestari, Vita M. Mudjiono Masa, Maria Margaretha S. Nogo Mulad, Iga Arziela Mulyadi, Reyz Pasenda Mutmainnah, Chaerun Nadhifa Salma Namira, Safa Marety Nanik Zubaidah Nina Dhaniar Nirawati Pribadi Nurshiffa Hanifatul Amrina Jaelani Oktaria, Wanda Pasenda Mulyadi, Reyz Pratiwi, Dian Dwi Rahardia, Nabiela Ramadani, Rania Rizka Riyadi, Tiara Lathifah Rizka Firdaus Rosidin Ruslan Effendy Ruslan Effendy Saravanan, Pravinna Sekar Firdhea Rizkifa Soetanto Setyabudi Sri Kunarti Sri Kunarti, Sri Sudarsono, Caeleb Fabrizio Sukaton, Sukaton Sutela, I Gede Marantika Yogananda Tanzil, Muhammad Iqbal Tasya Nafatila Veda Sahasika Azaria Nariswari Vina Zavira Nizar Vita Ariesta Vivian Widjaya, Olivia Wahab, Sofi Arnesti Widjaja, Olivia Vivian Wulandini, Raden Ajeng Yasmin Tasya Brilyanti Yonas, Yovita Yovita Yonas

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