Febriastuti Cahyani
Faculty Of Dental Medicine, Universitas Airlangga,Surabaya,Indonesia

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Anti-glucan effects of propolis ethanol extract on Lactobacillus acidophillus Ira Widjiastuti; Adioro Soetojo; Febriastuti Cahyani
Dental Journal (Majalah Kedokteran Gigi) Vol. 50 No. 1 (2017): March 2017
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (484.392 KB) | DOI: 10.20473/j.djmkg.v50.i1.p28-31

Abstract

Background: In deep dentinal caries cases, bacteria mostly found are Lactobacillus acidophilus classified as gram positive bacteria and as facultative aerobes producing glucosyltransferase (GTF) enzyme. GTF enzyme can alter sucrose into glucans. Glucan is sticky and insoluble in water. As a result, GTF enzyme can facilitate plaque formation and microorganism colonization on tooth surface. In addition, Lactobacillus acidophilus also can form acid leading to demineralization of organic and inorganic materials, resulting in dental caries. Multidrug-resistant phenomena, on the other hand, have led to the use of natural resources, one of which is propolis as an antimicrobial material and as a new anti-infective therapeutic strategy. Propolis is a resinous substances collected by worker bees (Apismellifera) from barks and leaves of plants. Propolis has a complex chemical composition and biological properties, such as antibacterial, antiviral, antifungal, anti-inflammatory, and antitumor. Purpose: This research aimed to reveal anti-glucan effects of propolis ethanol extract generated from honey bee, Apis mellifera spp on Lactobacillus acidophilus bacteria. Method: Before antiglucan test was conducted, glucan-formation test was performed on Lactobacillus acidophilus bacteria using SDSpage. Meanwhile, anti-glucan adhesion test on Lactobacillus acidophilus bacteria was carried by culturing the bacteria at 37ºC temperature in a jar with 10% CO2. Test tubes were placed at an angle of 30º for 18 hours to review the attachment of bacteria at the glass surfaces. After the incubation, the culture of bacteria was vibrated using a mixer vortex for a few minutes, and then cultured in solid MRS A media. Bacteria grown were measured by using colony counter. Result: The ethanol extract of propolis with a concentration of 1.56% was the lowest concentration inhibiting the attachment of glucan to Lactobacillus acidophilus bacteria. Conclusion: The ethanol extract of propolis with a concentration of 1.56% can be used as an anti-glucan material for Lactobacillus acidophilus bacteria.
Biocompatibility of 0,78% tannin of garciniamangostanalinnpericarp extract and 0,2% chlorhexidinegluconateagainst BHK-21 fibroblast cells culture Monika Werdiningsih; Ira Widjiastuti; Febriastuti Cahyani; Dian Agustin Wahjuningrum
Conservative Dentistry Journal Vol. 10 No. 1 (2020): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (588.872 KB) | DOI: 10.20473/cdj.v10i1.2020.36-39

Abstract

Background.Chlorhexidinegluconate is one of endodontic irrigants potential due to its antibacterial activity. Although it is an effective antibacterial agent, chlorhexidinegluconate cannotdissolve organic substances and necrotic tissue present in the root canal. In addition, same as other chemicals, chlorhexidinegluconate also cause a cytotoxic effect. Tannin extracts of mangosteenpericarp (Garciniamangostana Linn.) demonstrated various biological activities including antibacterial, antioxidant, anticancer and anti-inflammatory. Tannin extracts of mangosteenpericarp can be considered as an alternative endodontic irrigation for dental application.Purpose.The aim of this study was to compare the biocompatibility between 0,78% tannin extracts of mangosteen pericarp and 0,2% chlorhexidinegluconate to BHK-21 fibroblass cell. Method.Tannins obtained from extracts of mangosteenpericarp. Preliminary test was conducted in the biocompatibility of tannin extracts of mangosteenpericarp in various concentrations of the BHK-21 fibroblass. Tannins extracts of mangosteenpericarp was at concentrations of 0,78% less toxic than the other concentrations. Then biocompatibility of 0,78% tannin extracts of mangosteenpericarp compare with 0.2% chlorhexidinegluconate using cytotoxicity test on BHK-21 fibroblass cells with MTT method and observed by ELISA reader. Results.0,78% tannins extracts of  mangosteenpericarp showed good biocompatibility with fibroblass BHK-21 than 0,2% chlorhexidinegluconate. There was a significant differences between the results of treatment with 0,78% tannin extracts of mangosteenpericarp and 0.2% chlorhexidinegluconate. Conclusion. 0,78% tannin extracts of mangosteenpericarp has better biocompatibility than 0,2% chlorhexidinegluconate as an endodontic irrigants.
Single visit root canal treatment with post crown restoration Ismiyatin, Kun; Saraswati, Widya; Cahyani, Febriastuti; Vivian Widjaya, Olivia; Ghozali Pangestu, Dede; Arshila, Dea
Conservative Dentistry Journal Vol. 12 No. 2 (2022): July-December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v12i2.2022.57-62

Abstract

Background: Single visit endodontics has been shown to be an effective treatment modality when compared with multiple visit therapy and it does not deviate from achieving the objectives of proper biomechanical preparation, debridement, shaping, disinfection and 3 dimensional obturation of root canal system and is more beneficial to the patient and dentist provided there is careful case selection and strict follow of standard endodontic protocols. Symptomatic teeth can also be managed by single visit after controlling the abscess infection with antibiotic, provided the root canal is dry without any discharge.Purpose: This study aims to perform single visit root canals on teeth with irreversible pulpitis and restoration with post core and crown. Case(s): A 48-years-old female comes with dental complaints of upper left rear spontaneous pain at night. The tooth has had cavities since about two years ago and has never been previously treated. The patient said had not any systematic health disorder. There is a spontaneous pain that the patient feels. Case Management: From the examination that has been carried out, a clinical diagnosis of tooth 25 were symptomatic pulpitis irreversible with normal apical tissue. The restoration endodontic treatment plan was a single visit endodontic and the restoration is post core and crown. Conclusion: Symptomatic pulpitis irreversible with normal apical tissue treatment can be carried out single visit root canal treatment. Restoration of post endodontic treatment in this case need fiber post and core using composite and finally restoration using crown that made of zirconia. Zirconia has a higher level of material resistance than lithium disilicate. This is what makes zirconia the choice, especially for posterior teeth.
One Visit Endodontic with Internal Bleaching on Tooth 12 Post Trauma: A Case Report Pasenda Mulyadi, Reyz; Arnesti Wahab, Sofi; Cahyani, Febriastuti; Saraswati, Widya
Conservative Dentistry Journal Vol. 12 No. 2 (2022): July-December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v12i2.2022.63-67

Abstract

Introduction: Post-traumatic non-vital teeth is one of the indications for One Visit Endodontic. Trauma to the teeth may arise from conditions that cause the teeth to become necrotic so that the teeth will look darker and experience discoloration. Clinical conditions that indicate the death of the dental pulp and teeth require endodontic treatment. This situation will cause discoloration of the teeth that occur as a result of the metamorphosis of calcified pulp. Internal whitening is a treatment that can be done after the first treatment. Purpose: To provide information about discolored teeth management after trauma which causes pulp necrosis by doing root canal treatment and internal bleaching to change tooth color. Case: A 31-year-old woman came to the RSKGMP Universitas Airlangga with a referral from the orthodontics department where her teeth were darker in color and interfered with her appearance. In the previous history, the patient had an accident and trauma 10 years ago. Case Management: The first step is access opening and a good glide path. Root canal preparation was performed using the Crown Down Pressureless technique. The obturation technique used is Single Cone. The results were good and there were no symptoms during the control. Conclusion: One visit endodontic can be a treatment option for post-traumatic non-vital teeth. Internal bleaching with Walking Bleach Technique is also proven effective in restoring the original tooth color without excessive reduction of the dental crown.
Shock wave enhanced emission photoacoustic streaming (SWEEPS): An alternative to conventional irrigation in endodontic treatment Jordana, Jesslyn; Nicholas Tandean, Jason; Fiona Salim, Jennifer; Cahyani, Febriastuti; Sampoerno, Galih
Conservative Dentistry Journal Vol. 12 No. 2 (2022): July-December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v12i2.2022.68-72

Abstract

Background: Irrigation is an essential step in root canal treatment (RCT). However, the complex structure of the root canal can limit the penetration of irrigants, causing RCT failure. Shock wave enhanced emission of photoacoustic streaming (SWEEPS) has been developed to improve root canal cleaning by increasing the penetration of irrigants. SWEEPS utilized the Er:YAG laser to generate micropulse, producing bubbles delivered in liquid, causing powerful photodynamic streaming. However, its superiority compared to conventional irrigation remains unclear. Thus, we aim to review the available evidence to assess the benefits of SWEEPS over conventional irrigation. Purpose: This study aims to review the available evidence to assess the benefits of SWEEPS over conventional irrigation. Reviewa: Article searches were conducted on Pubmed, ScienceDirect, EBSCOhost, and ProQuest using SWEEPS and root canal irrigation as keywords. Articles were included if they aim to assess the benefits of SWEEPS over conventional irrigation in removing smear layer, debris, pulp tissues, or bacteria. A total of 833 articles were initially retrieved from systemic search of literature and 6 articles following inclusion criteria were included in the review. Out of 6 articles, 4 articles suggested that SWEEPS could increase the removal of smear layer, debris, pulp tissues, and bacteria in the root canal compared to conventional irrigation. However, 2 articles found contradictory results, in which SWEEPS and conventional irrigation had similar outcomes in terms of penetration depth and could not completely eradicate biofilm. Conclusion: This review suggests that SWEEPS offers more benefits over conventional irrigation since it can improve the removal of smear layer, debris, pulp tissues, and bacteria in the root canal.
BMP-7 Expression After Induction of Hydroxyapatite Bovine Tooth Graft Gel as A Socket Preservation of The Alveolar Bone (Laboratory Experimental of The Alveolar Bone) Zubaidah, Nanik; Namira, Safa Marety; Aryandhita, Amanda Paramitha Putri; Firyasasty, Beffano Roziq Herdymunas; Cahyani, Febriastuti
Conservative Dentistry Journal Vol. 13 No. 2 (2023): July-December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v13i2.2023.60-63

Abstract

Background: Hemisection is an endodontic surgical procedure that can leave alveolar bone defects. Changes in the dimensions of the alveolar bone can be minimized by preserving the alveolar bone socket using a bone graft. Induction of Hydroxyapatite Bovine Tooth Graft (HAp-BTG) in the alveolar bone socket is expected to increase the expression of BMP-7 which is a growth factor in osteogenesis. Purpose: To describe the increase in BMP-7 expression after induction of hydroxyapatite bovine tooth graft gel as a socket preservation of the alveolar bone of Wistar rats on the 14th and 28th days. Methods: 28 Wistar rats were extracted on the lower left incisor. Post-extraction sockets were induced by Polyethylene Glycol (PEG) as the control group and HAp-BTG + PEG as the treatment group. On the 14th and 28th day the Wistar rats were euthanized and the mandibles were taken. By using immunohistochemical staining on anatomic histopathological examination, BMP-7 expression was observed using a microscope with 1000í— magnification. The research results were analyzed using One Way ANOVA. Results: There was a significant increase in BMP-7 expression between the control group and the treatment group. Conclusion: BMP-7 expression after induction of hydroxyapatite bovine tooth graft gel as a socket preservation of the alveolar bone of Wistar rats increased on the 14th and 28th days.
Apexification with mineral trioxide aggregate on post trauma right maxillary central incivus teeth Masa, Maria Margaretha S. Nogo; Pratiwi, Dian Dwi; Cahyani, Febriastuti; Zubaidah, Nanik; Namira, Safa Marety; Wulandini, Raden Ajeng; Tanzil, Muhammad Iqbal; Oktaria, Wanda; Saraswati, Widya
Conservative Dentistry Journal Vol. 13 No. 2 (2023): July-December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v13i2.2023.81-84

Abstract

Background: Trauma often occurs in the maxillary anterior teeth in both children and adults. This usually causes the pulp of a tooth whose root is not yet fully formed to experience necrosis, and the apex closure stops causing the apex to be wide and open. Apexification is a method to induce a calcified barrier in necrotic roots with open apex. Treatment with Mineral trioxide aggregate (MTA) can shorten treatment time and has a higher long-term success rate than using Ca(OH)2. Purpose: The aim of this report is to describe apexification cases using MTA on a non-vital right maxillary central incisor. Case: A 21-year-old male patient came to the Dental Conservation Clinic at Airlangga University Dental Hospital with complaints of broken and discolored front teeth. The tooth was broken after falling ± 10 years ago (2013) while playing football, it was swollen and painful, but in the last ± 1 year (2022) it has never hurt again. The patient's medical history revealed no systemic abnormalities. Objective examination of tooth 11 class IV ellis fracture, negative percussion and bite test, surrounding normal gingiva, no mobility. The radiographic appearance of tooth 11 shows a wide open apex and a radiolucency at the root tip. Vitality of non-vital teeth, Diagnosis of pulpal necrosis with asymptomatic apical periodontitis. Case Management: In this case, the treatment plan is endodontic treatment in the form of apexification with MTA material. The obturation technique was performed using warm thermoplastic (backfill) and accompanied by a fixed restoration using a fiber post and all porcelain crown with a good prognosis for patient care. Conclusion: Apexification with MTA with post and IPS Emax crown restoration is a good treatment option for immature right maxillary central incisor with open apex. The patient is satisfied because the teeth can return both aesthetically and functionally according to the stomatognathic system.
A retreatment of an endodontic tooth 25 with underfilled Cahyani, Febriastuti; Adita, Farah Rahmi; Rachmawati, Atika Nisaa; Haq, Addina Ainul; Fadila, Okta Mulia Nur; Cinantya, Aulia Dian; Pribadi, Nirawati
Conservative Dentistry Journal Vol. 13 No. 2 (2023): July-December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v13i2.2023.85-90

Abstract

Background: Cases of endodontic failure requiring retreatment are endodontic failure cases in which microorganisms in root canal are able to survive, causing inflammation and pain. Purpose: This research aims to carry out retreatment of teeth after endodontic treatment to maintain and improve its function. Case: Male patient (67 years) has suffered from pain when chewing and discolored upper left second premolar tooth since one year ago in 2022. Ten years ago in 2013, he got dental treatment for the tooth. The patient also has a history of controlled hypertension. Case management: Several stages were conducted in sequence, namely gutta percha removal from previous endodontic treatment, root canal re-preparation, irrigation sequence, dressing, and obturation with single cone technique. Conclusion: In this case, an indirect restoration in the form of a crown should have been conducted to obtain a good contact point. As a result, food retention occurred in the interdental area, and then microorganisms entered through the periodontal tissue, causing periodontal inflammation. The inflammation then spread apically until it entered one-third of the apical in root canal, resulting in periapical inflammation. Exposed dentinal tubules in the missing portion of cementum then cause bacterial invasion of the tubules and increase the possibility of damage to the pulp. Periodontal disease can progress apically and involve the apical foramen. In this case, an indirect crown restoration was used since the quality of the final restoration has an impact on the survival and success rate of the endodontically treated tooth. In conclusion, a well-sealed crown restoration will prevent the entry of microorganisms
Anti-microbial efficacy of garlic (Allium Sativum L.) extract as a root canal irrigant in endodontic treatment Cahyani, Febriastuti; Farizaldi, Reisya Irfanny; Syafaati, Lailatul Maghfiroh; Hartanli, Audrey Luisa; Aryandhita, Amanda Paramitha Putri
Conservative Dentistry Journal Vol. 13 No. 2 (2023): July-December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v13i2.2023.91-95

Abstract

Background: Herbal medications are an effective alternative in dental endodontic treatment due to their antimicrobial, anti-inflammatory, and biocompatibility properties and thus they are more biocompatible and less cytotoxic. The antibacterial, antifungal, and antiviral properties of garlic (Allium sativum L.) support broad-spectrum activity against gram-positive and gram-negative organisms and effectiveness against multidrug-resistant organisms that are essential as endodontic materials. Purpose: The purpose of this literature review is to analyze how the antimicrobial efficacy of garlic (Allium sativum L.) extract can be used as a root canal irrigant in endodontic treatment. Review: The development of pulp and periapical disease involves high-intensity bacterial activity that proliferates and produces toxic side products such as organic acids. When crushed or minced, garlic releases allicin compounds through the enzymatic action of alliinase (cysteine sulfoxide lyase) on alliin. Allicin, organosulfur compounds, and antioxidant compounds in garlic extract can exert antimicrobial role by inhibiting the synthesis of pathogenic microorganisms, fighting the resulting infection, and working synergistically with other antimicrobial agents to increase the effectiveness of treatment against antibiotic- resistant bacterial infections. In comparison to other antimicrobial agents, a number of studies have shown that there is no significant difference between garlic extract and sodium hypochlorite (NaOCl) in exerting bactericidal effects against pathogenic bacteria. Other studies have also shown that garlic extract has good dentin penetration properties as well as ideal flow and wetting capabilities. Conclusion: Garlic (Allium sativum L.) extract has good antimicrobial efficacy, dentin penetration, as well as ideal properties of root canal irrigation in endodontic treatment
Differences of compressive strength between calcium carbonate from blood clam shells and calcium hydroxide as a candidate for pulp capping material Saraswati, Widya; Juniarti, Devi Eka; Lestari, Vita; Soetojo, Adioro; Kunarti, Sri; Cahyani, Febriastuti; Suardita, Ketut; Rahardia, Nabiela
Conservative Dentistry Journal Vol. 14 No. 1 (2024): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v14i1.2024.11-14

Abstract

Background: Pulp capping is one of the treatments for reversible pulpitis and aims to maintain pulp vitality. This treatment requires a material that can protect the pulp with good biocompatibility. The physical and mechanical properties, bio interactivity and bioactivity of pulp capping materials are very important for the formation of reparative dentin. Calcium hydroxide (Ca(OH)2) as the gold standard material in pulp capping treatment also has some disadvantages. Another alternative for pulp capping material is blood clam shell because it contains 98% calcium carbonate (CaCO3), which is a compound with a bone-like structure and can induce pulp cell differentiation. Objective: To investigate and explain the difference in compressive strength between CaCO3 from blood clam shells and Ca(OH)2 as a candidate pulp capping material. Methods: This research is a laboratory experimental study with post test only control group design method. Ca(OH)2 and CaCO3 samples were formed with a mixture of powder and aquadest with 4x6 mm sample size. The samples were dried at room temperature and the compressive strength was measured using a universal testing machine (UTM). Result: There is a significant difference in compressive strength between Ca(OH)2 and CaCO3 blood clam shells in the Mann-Whitney test results (p<0.05). Conclusion: The results of the compressive strength test between the mixture of Ca(OH)2 with aquadest in a ratio of 1:1 are greater than the mixture of CaCO3 blood clam shells with aquadest in a ratio of 3:1 so that pure CaCO3 blood clam shells with distilled water without other additives cannot be used as a candidate for capping pulp material.