Claim Missing Document
Check
Articles

Found 20 Documents
Search

UJI IN VITRO CARBONATE APATITE-CHITOSAN SCAFFOLDS SEBAGAI MATERIAL CANGKOK TULANG PADA TEKNIK REKAYASA JARINGAN: IN VITRO TEST OF APATITE - CHITOSAN CARBONATE SCAFFOLDS AS BONE GRAFT MATERIAL IN TISSUE ENGINEERING TECHNIQUES Maretaningtias Dwi Ariani; Anita Yuliati; Utari Kresnoadi
Dentika: Dental Journal Vol. 18 No. 1 (2014): Dentika Dental Journal
Publisher : TALENTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (135.174 KB) | DOI: 10.32734/dentika.v18i1.1921

Abstract

Untuk meningkatkan adhesi sel dan kemampuan pembentukan tulang dari kitosan, dicoba untuk membuat suatuscaffolds yang menggabungkan kitosan dengan carbonate apatite (CA). Tujuan penelitian ini adalah untukmembuat carbonate apatite-chitosan scaffolds (CA-ChSs) serta mengevaluasi CA-ChSs dari sudut pandangproliferasi sel menggunakan MC3T3-E1. Chitosan scaffolds (ChSs) yang berisi 25, 50, 75, 100, 125, 150, 200dan 400 mg bubuk kitosan (100D, YSK, Japan) dibuat dengan prosedur dilarutkan dalam 5 ml asam asetat 2%,dikocok selama 15 menit, kemudian dinetralkan dengan 15 ml 0,1 M larutan NaOH. Setelah disentrifugasi pada1500 rpm selama 10 menit, kelebihan air dibuang, kemudian gel kitosan dikemas ke dalam cetakan teflon(diameter: 5 mm, tinggi: 2 mm). Cetakan dibekukan pada suhu -80°C selama 2 jam dan dikeringkan dalammesin beku kering pada suhu -54°C selama 24 jam. Selanjutnya dilakukan radiasi ultraviolet pada ChSs selama 2jam. Untuk membuat CA-ChSs, dipilih ChSs yang berisi 200 mg bubuk kitosan. Setelah dinetralisir, 10, 50, 100,200 dan 300 mg dari 0,06 M CA ditambahkan ke dalam kitosan gel yang berisi 200 mg bubuk kitosan. StrukturCA-ChSs diamati dengan scanning electron microscope (SEM). Proliferasi MC3T3-E1 dalam ChSs dan CAChSs dievaluasi pada hari ke-1, 7 dan 14. CA-ChSs dengan struktur tiga dimensi yang berpori serta adanya perlekatan CA dapat diamati dengan jelas menggunakan SEM. Hasil penelitian menunjukkan jumlahpertumbuhan dan perkembangan sel pada CA-ChSs secara signifikan lebih banyak dibandingkan pada ChSs(kontrol) pada setiap tahap pengamatan di hari ke-1, 7 dan 14 (p< 0,05). Sebagai kesimpulan, CA-ChSs adalahkandidat untuk material cangkok tulang pada teknik rekayasa jaringan.
AKTIVITAS EXPRESSI KOLAGEN II DAN OSTEOCALSIN TULANG ALVEOL AKIBAT PRESERVASI SOKET PENCABUTAN GIGI DENGAN CAMPURAN ALOE VERA DAN GRAFT 0,5%: EXPRESSION ACTIVITY OF COLLAGEN II AND OSTEOCALCYN ALVEOLAR BONE QONSEQUENT PRESERVATION POST EXTRACTION SOCKET FILLED WITH A MIXTURE OF ALOE VERA AND GRAFT 0,5% Utari Kresnoadi; Retno Pudji Rahayu; Eha Djulaeha
Dentika: Dental Journal Vol. 18 No. 1 (2014): Dentika Dental Journal
Publisher : TALENTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (511.98 KB) | DOI: 10.32734/dentika.v18i1.2005

Abstract

Aloe vera adalah tanaman yang mempunyai fungsi antibiotik, anti inflamasi, anti jamur dan bahan yang merupakanbiogenik stimulator untuk merangsang bovine graft agar mempercepat pertumbuhan tulang. Tujuan penelitian ini adalahuntuk mengetahui mekanisme aktivitas osteoblas pada proses pembentukan tulang alveol dengan pemberian kombinasiAloe vera dan XCB. Metode penelitian adalah dengan melakukan uji laboratoris dengan hewan coba: 54 ekorcaviacabaya, dibagi menjadi 9 kelompok, masing-masing 6 ekor, terdiri atas 3 group, yang pertama kelompok kontrolyaitu hanya dicabut saja tanpa perlakuan, kelompok 2 adalah kelompok yang setelah gigi dicabut diberi XCB saja dankelompok ke 3 adalah kelompok yang setelah pencabutan diberi campuran Aloe vera dengan XCB pada soket bekaspencabutan gigi. Pemeriksaan dilakukan setelah 14, 30 dan 60 hari. Kemudian diperiksa ekspresi osteocalcin dan kolagentipe II sebagai marker terjadinya tulang alveol dengan pemeriksaan imunohistokimia. Hasil penelitian menunjukkanterdapat perbedaan bermakna ekpresi osteocalcin dan collagen II antara kelompok kontrol dengan kelompok yang diberiAloe vera + XCB pada, 14, 30 dan 60 hari setelah pencabutan. Sebagai kesimpulan, penelitian aktivitas pertumbuhanosteoblas pada tulang alveol ditandai dengan peningkatan ekpresi osteocalcin dan kolagen II.
Nutrition education and mastication improvement through social services provision in Ketapang sub-district, Probolinggo Kresnoadi, Utari; Kamadjaja, Michael Josef Kridanto; Ari, Muhammad Dimas Aditya
Indonesian Journal of Prosthodontic Vol 1, No 2 (2020): December 2020
Publisher : Indonesia Prosthodontic Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (524.245 KB) | DOI: 10.46934/ijp.v1i2.24

Abstract

Introduction: A community-based exercise was implemented with the specific theme “Awareness raising among Family Welfare Program and Youth Organization group members of the importance of Family Nutrition Literacy and of nutrition education and mastication improvement through provision of removable dentures” in Ketapang sub-district, Kademangan district, Probolinggo. Aim: This community-based service was intended to inform the public of the importance of nutritional consumption to ensure a well-nourished family and enhanced mastication resulting from the use of removable dentures. This event was held in the middle of the COVID-19 pandemic. Methods: Two teams managing innovative and promotion were created. The role of the former was to conceptualize activities, while that of the latter was to directly inform the target community using a combination of printed and electronic media. These teams consisted of lecturers and 2nd year residents of the Department of Prosthodontics, Faculty of Dentistry, Universitas Airlangga. Results: The event involved the use of video conferencing through which residents delivered online counselling session to Family Welfare Program, Youth Organization group, and health cadre mem-bers who had already gathered at Ketapang Health Center, while maintaining physical distancing. Several residents worked on site alongside health cadres from the Ketapang Health Center. Given the ongoing pandemic, the teams decided to donate certain props: four full dentures, four removable partial dentures and four fixed partial dentures. Conclusion: Online counseling of the Ketapang Health Center cadres was expected to increase their awareness and comprehension of nutrition education and improvement in the mastication function of dentures through the use of teaching aids.
Combination of Aloe vera and xenograft induction on decreasing of NF-kb of tooth extraction socket preservation in Cavia cobaya Utari Kresnoadi; Retno Pudji Rahayu
Dental Journal (Majalah Kedokteran Gigi) Vol. 47 No. 1 (2014): March 2014
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (745.446 KB) | DOI: 10.20473/j.djmkg.v47.i1.p1-6

Abstract

Background: Tooth extraction can naturally cause inflammation triggering osteoclast proliferation and alveolar bone resorption. Preservation of the tooth extraction sockets is needed for patients in order to reduce alveolar bone resorption risks. Aloe vera is known to have anthraquinones components, namely Aloin, Aloe emedin, and barbaloin, considered as anti-inflammation. Therefore, to overcome the inflammation, the role of NF-kb is very significant to decrease nuclear factor kappa b (NF-kb). As a result, inflammation risks will be decreased. Purpose: The study was aimed to determine the induction effect of combination of Aloe vera and XCB into tooth extraction sockets to reduce inflammation by reducing NF-kb expression, osteoclasts and osteoblasts. Methods: Forty-eight Cavia cobaya were divided into eight groups, each group consisted of six animals. The mandibular incisors of those Cavia cobaya were extracted and induced with either PEG, XCB, Aloe vera, or the combination of Aloe vera + XCB. Those animals were sacrificed on day 7 and day 30 after the extraction. Then immunohistochemical and histopathology examinations were conducted to observe NF-kb expression, osteoblasts and osteoclasts. Results: It was known that in group induced with the combination of Aloe vera and xenograft concelous bovine, the growth of osteoblasts was high, while NF-kb expression and osteoclasts reduced. Conclusion: It can be concluded that the induction of the combination of Aloe vera and XCB into the tooth extraction sockets can reduce NF-kb expression and osteoclast, as a result, alveolar bone resorption risks decrease, and osteoblast increase.Latar belakang: Trauma mekanis akibat pencabutan gigi asli menyebabkan keradangan. Keradangan memicu proliferasi osteoklas sehingga menyebabkan resorpsi tulang alveolararis. Pada pembuatan gigi tiruan, resorpsi tulang alveolar yang terjadi, sangat tidak diinginkan, sebab resorpsi tulang alveolar mengurangi keberhasilan pembuatan gigitiruan. Diperlukan preservasi soket pencabutan gigi asli pada penderita untuk mencegah terjadinya resorpsi tulang alveolar. Aloe vera mempunyai komponen anthraquinon yaitu aloin, Aloe emodin, barbaloin yang merupakan anti inflamasi yang dapat secara cepat menyembuhkan luka, sehingga berpotensi untuk digunakan pada preservasi soket. Didalam mengatasi keradangan peran NF-kb sangat berarti, sebab penurunan NF-kb akan mengurangi terjadinya inflamasi. tujuan: Penelitian ini bertujuan untuk menguji apakah induksi kombinasi lidah buaya dan XCB ke soket pencabutan gigi dapat mengurangi peradangan dengan mengurangi ekspresi NF - kb , osteoklas dan osteoblast. Metode: Empat puluh delapan ekor Cavia cabaya yang terdiri dari 8 kelompok, tiap kelompok 6 ekor, kelompok pengisian PEG (kontrol), kelompok pengisian XCB, kelompok pengisian aloe vera dan kelompok pengisian kombinasi aloe vera dan XCB, kelompok ini terdiri dari kelompok 7 dan 30 hari, kemudian diperiksa dengan imunohistokimia ekspresi NF-kb dan pemeriksaan histologi untuk osteoblas dan osteoklas. Hasil: Kelompok yang diisi kombinasi Alo vera dan xenograft concelous bovine pada soket pencabutan gigi, menunjukan nilai tertinggi dalam pertumbuhan osteoblas dan penurunan pada ekspresi NF-kb dan osteoklas. Simpulan: Induksi kombinasi Aloe vera dan xenograft concelous bovine pada preservasi soket pencabutan gigi dapat menurunkan ekspresi NF-kB dan osteoklas, menurunkan resiko resorpsi tulang alveolar dan meningkatkan osteoblas.
The increasing of fibroblast growth factor 2, osteocalcin, and osteoblast due to the induction of the combination of Aloe vera and 2% xenograft concelous bovine Utari Kresnoadi
Dental Journal (Majalah Kedokteran Gigi) Vol. 45 No. 4 (2012): December 2012
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1385.511 KB) | DOI: 10.20473/j.djmkg.v45.i4.p228-233

Abstract

Background: To make a successfull denture prominent ridge is needed, preservation on tooth extraction socket is needed in order to prevent alveol bone resorption caused by revocation trauma. An innovative modification of the material empirically suspected to be able reduce inflammation caused by the revocation trauma is a combination of Aloe vera and xenograft concelous bovine (XCB) and Aloe vera is a biogenic stimulator and accelerating the growth of alveolar ridge bone after tooth extraction. Purpose: The research was aimed to determine of the increasing alveol bone formation by inducing the combination of Aloe vera and 2% xenograft concelous bovine. Methods: To address the problems, the combination of Aloe vera and xenograft concelous bovine was induced into the tooth extraction sockets of Cavia cabayas which devided on 8 groups. Groups control, filled with XCB, Aloe vera and Aloe vera and XCB combination, at 7 days and 30 days after extraction. Afterwards, immunohistochemical examination was conducted to examine the expressions of FGF-2 and osteocalcin, as the product of the growth of osteoblasts. Results: There were significantly increases expression of FGF-2 and osteocalcyn on group which filled with XCB, Aloe vera and combined Aloe vera and XCB. Conclusion: It may be concluded that the induction of the combination of Aloe vera and xenograft concelous bovine into the tooth sockets can enhance the growth expressions of FGF-2 and osteocalcin as the product of osteoblasts, thus, the growth of alveolar bone was increased.Latar belakang: Untuk keberhasilan pembuatan gigitiruan diperlukan ridge yang prominent, maka diperlukan suatu preservasi soket pencabutan gigi untuk mencegah terjadinya resopsi tulang alveolar akibat trauma pencabutan. Suatu inovasi modifikasi bahan yang diduga secara empiris dapat mengurangi keradangan karena trauma pencabutan adalah berupa kombinasi Aloe vera dan xenograft concelous bovine (XCB). Aloe vera yang merupakan biogenik stimulator untuk merangsang dan mempercepat pertumbuhan tulang alveolar setelah pencabutan gigi. Tujuan: untuk membuktikan bahwa induksi kombinasi Aloe vera dan xenograft concelous bovine 2% pada soket pencabutan gigi, dapat meningkatkan pembentukan tulang alveolar. Metode: Pengisian kombinasi Aloe vera dan XCB pada soket pencabutan gigi Cavia cabaya yang dibagi dalam 8 kelompok. Kelompok: kontrol, Aloe vera, XCB, kombinasi Aloe vera dan XCB, masing-masing dalam pemeriksaan 7 dan 30 hari. Kemudian dilakukan pemeriksaan imunohistokimia ekspresi FGF-2 dan osteocalcyn sebagai produk pertumbuhan sel osteoblas. Hasil: Terdapat peningkatan ekspresi FGF-2 dan osteocalcyn pada kelompok yang diisi XCB, Aloe vera dan kombinasi Aloe vera + XCB dibanding dengan kelompok kontrol. Kesimpulan: Induksi kombinasi Aloe vera dan xenograft concelous bovine 2% pada soket pencabutan gigi dapat, meningkatkan ekspresi FGF-2 dan osteocalcyn, sehingga dapat meningkatkan pertumbuhan tulang alveol.
Stimulation of osteoblast activity by induction of Aloe vera and xenograft combination Utari Kresnoadi; Retno Pudji Rahayu
Dental Journal (Majalah Kedokteran Gigi) Vol. 44 No. 4 (2011): December 2011
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (291.857 KB) | DOI: 10.20473/j.djmkg.v44.i4.p200-204

Abstract

Background: Tooth extraction is generally followed by alveolar ridge resorption that later can cause flat ridge. Aloe vera have biogenic stimulator and hormone activities for wound healing. Purpose: This study was aimed to know osteoblast activities in alveolar bone after induction of Aloe vera and XCB combination. Methods: Fifty four of Cavia cabaya were divided into three main groups. Group I was control group. Group II was filled with xenograft concelous bovine (XCB) and group III was filled with the combination of Aloe vera gel and XCB. Then, each group was divided into three sub groups according to timing, they are 14, 30, and 60 days after tooth extraction and application. Histology and morphology examination were performed on the harvested specimens. Results: There were significant differences between the control group and the other groups filled with the combination of Aloe vera and XCB. Conclusion: In conclusion, the application of Aloe vera gel and xenograft combination decrease the number of osteoclast and increase the number of osteoblast in post tooth extraction alveolar bone structure indicating the new growth of alveolar bone.Latar belakang: Pencabutan gigi pada umumnya selalu diikuti resopsi tulang alveolar, sehingga bila terjadi dalam waktu yang lama ridge akan menjadi flat. Aloe vera adalah bahan stimulasi biogenik dan mempunyai aktivitas hormon untuk proses penyembuhan luka. Tujuan: Tujuan dari penelitian ini adalah untuk mengetahui aktivitas osteoblas pada tulang alveol dengan pemberian kombinasi Aloe vera gel dan xenograft concelous bovine (XCB). Metode: Lima puluh empat ekor Cavia cabaya, dibagi menjadi 3 kelompok besar, kelompok pertama adalah kelompok kontrol yaitu hanya dilakukan pencabutan saja tanpa perlakuan, kelompok ke-2 yaitu kelompok yang setelah dicabut diberi XCB saja dan kelompok ke-3 yaitu kelompok yang setelah pencabutan diberi kombinasi Aloe vera gel dengan XCB pada luka bekas pencabutan gigi. Kemudian masing-masing kelompok besar ini dibagi lagi berdasarkan waktu menjadi 3 sub kelompok yaitu setelah 14, 30 dan 60 hari. Kemudian dilakukan pemeriksaan histology dan morfologi pada specimen hewan coba. Hasil: Terdapat perbedaan bermakna antara kelompok kontrol dan kelompok yang diberi kombinasi Aloe vera dan XCB. Kesimpulan: Disimpulkan bahwa pemberian kombinasi Aloe vera gel dan xenograft menyebabkan penurunan jumlah osteoklas dan peningkatan jumlah osteoblas pada struktur tulang alveol pasca pencabutan gigi yang menunjukkan adanya pertumbuhan tulang alveol baru.
Aesthethic and masticatory rehabilitation on post mandibular resection with combination of hollow obturator and hybrid prosthesis Arif Rachman; Eha Djulaeha; Utari Kresnoadi
Dental Journal (Majalah Kedokteran Gigi) Vol. 42 No. 2 (2009): June 2009
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (966.773 KB) | DOI: 10.20473/j.djmkg.v42.i2.p76-81

Abstract

Background: Replacing tooth lost caused by caries, periodontal disease, trauma and neoplasm including ameloblastoma which requires mandibular resection is important. Purpose: The aim of the study to rehabilitation of post mandibular resection with combination of hollow obturator and hybrid prosthesis. Case: A patient 25 years old, male, for having prosthesis to cover defect due to post right mandibular resection. Case Management: In this presented case, mandibular plate was applied due to spreading defect with losing almost a half body of mandible (class II modification 2 according to cantor and curtis classification). The design of therapy was mandibular obturator using hybrid prosthesis (removable partial denture metal frame and fixed splint crown with precision attachment) with hollow obturator. The application was based on several advantages: good aesthetic performance, retention, stability, lighter weight and equal share of vertical load for teeth on non surgical site. The result of control I, II, III, showed that aesthetic performance, masticatory function, speech and swallowing were in good condition. Conclusion: The design of mandibular obturator using hybrid denture with hollow obturator could rehabilitate aesthetic performance, masticatory function, speech and swallowing for patient with post mandibular resection.
Closed mouth method with dynamic and muco compressive impression on upper and lower jaw flat ridges for aid full denture retention Utari Kresnoadi; Rostiny Rostiny
Dental Journal (Majalah Kedokteran Gigi) Vol. 40 No. 4 (2007): December 2007
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (440.749 KB) | DOI: 10.20473/j.djmkg.v40.i4.p193-197

Abstract

A patient with flat ridge difficult to have retentive complete denture. The aim of this paper is to describe the combination of impression using closed mouth technique with dynamic and muco compressive material. In this case, the combination technique of dynamic impressive material and muco compressive material with closed mouth method on patient with upper and lower jaw flat ridges. The patient has made complete denture 10 times but not satisfied. The treatment of upper and lower flat ridges using this technique resulted retentive, stable and comfortable denture.
The change of temperature on the shear strength of permanent soft-liner on acrylic resin Waloejo Noegroho; Utari Kresnoadi; Adi Subianto
Dental Journal (Majalah Kedokteran Gigi) Vol. 39 No. 2 (2006): June 2006
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (191.558 KB) | DOI: 10.20473/j.djmkg.v39.i2.p77-79

Abstract

The characteristics of dental material such as solubility and water resorption, the use of adhesive, storage condition or used as thermo cycling or elevated-temperature are factors that can effect bond strength. The purpose of this study was to investigate the change of temperature on the shear strength of permanent soft liner on acrylic resin. Twenty-four specimens were divided into 3 groups and immersed in water at: 5 °C, 37 °C and 55 °C. Autograph AG 10 TE Shimadzu was used to determine the shear strength. The statistical test (ANOVA and LSD); showed that there were significant differences between temperature groups. The shear strength of 37 °C was higher than the temperature of 5 °C and 55 °C.
Effect of mangosteen peel extract combined with demineralized freezed-dried bovine bone xenograft on osteoblast and osteoclast formation in post tooth extraction socket Utari Kresnoadi; Yurike Hadisoesanto; Harly Prabowo
Dental Journal (Majalah Kedokteran Gigi) Vol. 49 No. 1 (2016): March 2016
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (493.675 KB) | DOI: 10.20473/j.djmkg.v49.i1.p43-48

Abstract

Background: Tooth extraction, a common procedure in dentistry, can cause bone resorption during socket healing. Therefore, it is important to perform socket preservation procedure to maintain alveolar bone. Providing a combination of mangosteen peel extract with demineralized freezed-dried bovine bone xenograft (DFDBBX) in tooth extraction socket was expected to accelerate alveol bone formation. Purpose: This study aims to determine the effect of mangosteen peel extract combined with DFDBBX introduced into the socket of post tooth extraction on the formation of osteoblasts and osteoclasts. Method: Twenty-eight (28) Cavia cobayas were divided into four groups. Extraction to the lower left incisor of Cavia cobaya was performed. The extraction socket was filled with 25 gram of PEG (group I) as a control, active materials consisted of mangosteen peel extract and DFDBBX 0.5% (group II), active materials consisted of mangosteen peel extract and DFDBBX 1% (group III), and active materials consisted of mangosteen peel extract and DFDBBX 2% (group IV). After thirty days, those Cavia cobayas were sacrificed. By using HE on Histopatological examination, the number of osteoblasts and osteoclasts were measured by light microscope with 400 times of magnification. The statistical analysis was then performed using oneway Anova & TukeyHSD test. Result: The component active materials consisted of mangosteen peel extract and DFDBBX 2% had the most significant results related to the formation of osteoblasts and osteoclasts. Conclusion: Mangosteen peel extract combined with DFDBBX can increase osteoblasts and decrease osteoclasts in the socket of tooth extraction in Cavia cobaya. The combination of mangosteen peel extract and DFDBBX 2% is the most effective material in increasing osteoblast and decreasing osteoclast.