Garuda - Garba Rujukan Digital

UJI IN VITRO CARBONATE APATITE-CHITOSAN SCAFFOLDS SEBAGAI MATERIAL CANGKOK TULANG PADA TEKNIK REKAYASA JARINGAN: IN VITRO TEST OF APATITE - CHITOSAN CARBONATE SCAFFOLDS AS BONE GRAFT MATERIAL IN TISSUE ENGINEERING TECHNIQUES Maretaningtias Dwi Ariani; Anita Yuliati; Utari Kresnoadi
Dentika: Dental Journal Vol. 18 No. 1 (2014): Dentika Dental Journal
Publisher : TALENTA

Untuk meningkatkan adhesi sel dan kemampuan pembentukan tulang dari kitosan, dicoba untuk membuat suatuscaffolds yang menggabungkan kitosan dengan carbonate apatite (CA). Tujuan penelitian ini adalah untukmembuat carbonate apatite-chitosan scaffolds (CA-ChSs) serta mengevaluasi CA-ChSs dari sudut pandangproliferasi sel menggunakan MC3T3-E1. Chitosan scaffolds (ChSs) yang berisi 25, 50, 75, 100, 125, 150, 200dan 400 mg bubuk kitosan (100D, YSK, Japan) dibuat dengan prosedur dilarutkan dalam 5 ml asam asetat 2%,dikocok selama 15 menit, kemudian dinetralkan dengan 15 ml 0,1 M larutan NaOH. Setelah disentrifugasi pada1500 rpm selama 10 menit, kelebihan air dibuang, kemudian gel kitosan dikemas ke dalam cetakan teflon(diameter: 5 mm, tinggi: 2 mm). Cetakan dibekukan pada suhu -80Â°C selama 2 jam dan dikeringkan dalammesin beku kering pada suhu -54Â°C selama 24 jam. Selanjutnya dilakukan radiasi ultraviolet pada ChSs selama 2jam. Untuk membuat CA-ChSs, dipilih ChSs yang berisi 200 mg bubuk kitosan. Setelah dinetralisir, 10, 50, 100,200 dan 300 mg dari 0,06 M CA ditambahkan ke dalam kitosan gel yang berisi 200 mg bubuk kitosan. StrukturCA-ChSs diamati dengan scanning electron microscope (SEM). Proliferasi MC3T3-E1 dalam ChSs dan CAChSs dievaluasi pada hari ke-1, 7 dan 14. CA-ChSs dengan struktur tiga dimensi yang berpori serta adanya perlekatan CA dapat diamati dengan jelas menggunakan SEM. Hasil penelitian menunjukkan jumlahpertumbuhan dan perkembangan sel pada CA-ChSs secara signifikan lebih banyak dibandingkan pada ChSs(kontrol) pada setiap tahap pengamatan di hari ke-1, 7 dan 14 (p< 0,05). Sebagai kesimpulan, CA-ChSs adalahkandidat untuk material cangkok tulang pada teknik rekayasa jaringan.

Novel development of carbonate apatite-chitosan scaffolds based on lyophilization technique for bone tissue engineering Maretaningtias Dwi Ariani
Dental Journal (Majalah Kedokteran Gigi) Vol. 45 No. 3 (2012): September 2012
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: The natural biopolymer chitosan (Ch) is currently regarded as a candidate for bone tissue engineering. However, Ch is poor for cell adhesion and low bone formation ability. In order to enhance cell adhesion and bone formation ability, combination of Ch with carbonate apatite (CA) was developed. Purpose: The aim of this study was to make carbonate apatite-chitosan scaffolds (CAChSs) and evaluate its osteoconductivity in terms of cell proliferation. Methods: Chitosan scaffolds (ChSs) were made by the following procedure. Twenty-five, 50, 100, 200 and 400 mg Ch was dissolved into 5 ml of 2% acetic acid (CH3COOH), shaked for 15 min and neutralized with 15 ml of 0.1 M sodium hydroxide (NaOH) solution. After centrifugation, Ch gel was packed into the molds then frozen at -80°C for 2h and dried in a freeze dry machine for 24h. The sponges were subjected to UV radiation for 2h. To make CA-ChSs, 200 mg Ch was selected. After neutralization, 50 mg of 0.06 M CA were added into the 200 mg Ch gel. The structure of CA-ChSs was observed by scanning electron microscope (SEM). Mouse osteoblast-like cell (MC3T3-E1) proliferation in these scaffolds was investigated at 1, 7, 14 and 21 days. Results: Three dimensional porous structures of CA-ChSs were clearly observed by SEM. Proliferated cell numbers in CA-ChSs was significantly higher than those in ChSs (control) at each stage (p<0.05). Conclusion: It can be concluded that newly developed CA-ChSs had three-dimensional interconnected porous structure, good handling property and supporting ability of proliferation of osteoblasts. It is suggested that newly developed CA-ChSs could be considered as a scaffolds material for bone tissue enginearing.Latar belakang: Kitosan yang merupakan biopolimer alami dianggap sebagai salah satu kandidat untuk rekayasa jaringan tulang. Namun, kitosan memiliki kelemahan terhadap adhesi sel dan kurang mampu membentuk tulang yang cukup. Untuk meningkatkan adhesi sel dan kemampuan pembentukan tulang, telah dikembangkan suatu scaffolds yang menggabungkan kitosan dengan carbonate apatite (CA). Tujuan: Penelitian ini bertujuan untuk membuat carbonate apatite-chitosan scaffolds (CA-ChSs) serta mengevaluasi osteokonduktivitas CA-ChSs dari sudut pandang proliferasi sel. Metode: Chitosan scaffolds (ChSs) dibuat dengan prosedur berikut ini. Dua puluh lima, 50, 100, 200 dan 400 mg bubuk kitosan dilarutkan dalam 5 ml asam asetat (CH3COOH) 2%, dikocok selama 15 menit dan dinetralkan dengan 15 ml 0,1 M larutan sodium hidroksia (NaOH). Setelah sentrifugasi, gel kitosan dikemas ke dalam cetakan teflon kemudian dibekukan pada suhu -80°C selama 2 jam dan dikeringkan dalam mesin beku kering pada suhu -54°C selama 24 jam. Selanjutnya dilakukan radiasi ultraviolet pada ChSs selama 2 jam. Untuk membuat CA-ChSs, dipilih ChSs yang berisi 200 mg bubuk kitosan. Setelah dinetralisir, 50 mg dari 0.06 M CA ditambahkan ke dalam kitosan gel yang berisi 200 mg bubuk kitosan. Struktur CA-ChSs diamati dengan scanning electron microscope (SEM). Proliferasi mouse osteoblast-like cell (MC3T3-E1) dalam ChSs dan CA-ChSs dievaluasi pada hari ke-1, 7 dan 14. Hasil: CA-ChSs dengan struktur tiga dimensi yang berpori dapat diamati dengan jelas menggunakan SEM. Jumlah pertumbuhan dan perkembangan sel pada CA-ChSs secara signifikan lebih banyak dibandingkan pada ChSs (kontrol) pada setiap tahap pengamatan di hari ke-1, 7, 14 dan 21 (p<0.05). Kesimpulan: Dapat disimpulkan bahwa CA-ChSs mempunyai struktur tiga dimensi dengan pori-pori yang saling berhubungan satu sama lain dan dapat meningkatkan proliferasi osteoblast. Hal ini menunjukkan bahwa CA-ChSs adalah kandidat untuk rekayasa jaringan tulang.

Toxicity testing of chitosan from tiger prawn shell waste on cell culture Maretaningtias Dwi Ariani; Anita Yuliati; Tokok Adiarto
Dental Journal (Majalah Kedokteran Gigi) Vol. 42 No. 1 (2009): March 2009
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: A biomaterial used in oral cavity should not become toxic, irritant, carcinogenic, and allergenic. Chitosan represents a new biomaterial in dentistry. Purpose: To examine the toxicity of chitosan from tiger prawn shell waste on cell culture with MTT assay. Methods: Chitosan with concentration of 0.25%, 0.5%, 0.75% and 1% was used in this experiment. Each sample was immersed on eppendorf microtubes containing media culture. After 24 hours, the immersion of media culture was used to examine the toxicity effects on BHK-21 cell based on MTT assay method. The density of optic formazan indicates the number of living cells. All data were then statistically analyzed by one-way Anava. Results: The number of living cells in chitosan from tiger prawn shell waste was 93.16%; 85.07%; 78.48%; 75.66%. Thus, there was no significant difference among groups. Conclusion: Chitosan with 0.25%, 0.5%, 0.75% and 1% concentrations from tiger prawn shell waste were not toxic for BHK-21 cell culture when using parameter CD50.

Application of chitosan scaffolds on vascular endothelial growth factor and fibroblast growth factor 2 expressions in tissue engineering principles Ariyati Retno Pratiwi; Anita Yuliati; Istiati Soepribadi; Maretaningtias Dwi Ariani
Dental Journal (Majalah Kedokteran Gigi) Vol. 48 No. 4 (2015): December 2015
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Tissue engineering has given satisfactory results as biological tissue substitutes to restore, replace, or regenerate tissues that have a defect. Chitosan is an organic biomaterial often used in the biomedical field. Chitosan has biocompatible, antifungal, and antibacterial properties. Chitosan is osteoconductive, suitable for bone regeneration applications. Bone defect healing begins with inflammatory phase as a response to the presence of vascular injury, so new vascularization is required. Vascular endothelial growth factor (VEGF) and basic fibroblast growth factor-2 (FGF2) are indicators of the beginning of bone regeneration process, playing an important role in angiogenesis. Purpose: This research was aimed to determine the effects of chitosan scaffold application on the expressions of VEGF and FGF2 in tissue engineering principles. Method: Chitosan was dissolved in CH3COOH and NaOH to form a gel. Chitosan gel was then printed in mould to freeze dry for 24 hours. Those rats with defected bones were divided into two groups. Group 1 was the control group which defected bones were not administrated with chitosan scaffolds. Group 2 was the treatment group which defected bones were administrated with chitosan scaffolds. Those rats were sacrificed on day 14. Tissue preparations were made, and then immunohistochemical staining was conducted. Finally, a statistical analysis was conducted using Kruskal Wallis test. Result: There was no significant difference in the expressions of VEGF and FGF2 between the control group and the treatment group (p>0.05). Conclusion: Chitosan scaffolds do not affect the expressions of VEGF and FGF2 during bone regeneration process on day 14 in tissue engineering principles

Scaffold combination of chitosan and collagen synthesized from chicken feet induces osteoblast and osteoprotegerin expression in bone healing process of mice Saka Winias; Diah Savitri Ernawati; Maretaningtias Dwi Ariani; Retno Pudji Rahayu
Dental Journal (Majalah Kedokteran Gigi) Vol. 50 No. 2 (2017): June 2017
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Over 500.000 of the 2,3 million surgical treatments requiring bone grafting procedures that are performed annually are likely to be necessitated by or will result in bone defects that will not regenerate. Treatment to regenerate new tissues is needed, especially for hard tissue repair, which not only relies on a natural osseointegration process, but also requires a physical support to guide the differentiation and proliferation of cells into the targeted functional tissue. Chitosan and collagen extracted from chicken feet combinations are expected to enhance the bioactive surface and provide mechanical strength as a bone graft scaffold. Purpose: The aim of this study was to investigate the role of chitosan and collagen scaffold synthesized from chicken feet applications to increase the expression of Osteoprotegerin (OPG) and osteoblast cells on the fourteenth day of bone healing. Methods: Eighteen three-month old, adult, male, Rattus novergicu strain rodents with a body weight ranging from 200-350 g were kept under controlled environmental conditions. The mice were randomly divided into three groups consisting of three subjects, each treated with collagen, chitosan, chitosancollagen combination (50:50) scaffolds. On the 14th post-treatment day, three members of each group were sacrificed. Examination of Osteoprotegerin (OPG) expression was conducted by means of immunohistochemistry staining with anti-OPG polyclonal antibodies. Meanwhile, osteoblast cell examination was performed by means of hematoxilin-eosin (HE) staining. Results: The mice treated with collagen and a chitosan-collagen combination scaffold presented an increase in the expression of Osteoprotegerin (OPG) and the number of osteoblast cells respectively. Conclusion: A combination of chitosan-collagen (50:50) scaffold extracted from chicken feet increased the expression of OPG and the number of osteoblasts in the bone healing process. The combination scaffolds demonstrated the highest OPG expression and number of osteoblasts compared to the other groups.

Improving Dental and Oral Health Through Empowering Village Youth to Enhance Awareness Regarding the Importance of Dentures Prabowo, Harly; Ariani, Maretaningtias Dwi; Kurdi, Abil; Rahmania, Primanda Nur; Sona, Nabila; Imani, Aurellia Della; Indrarini, Ratri; Wicaksono, Haryo Adhiweno; Ryani, Adelisa Devita
Indonesian Journal of Dental Medicine Vol. 7 No. 2 (2024): Indonesian Journal of Dental Medicine
Publisher : Faculty of Dental Medicine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijdm.v7i2.2024.49-51

Background: Dental and oral health, which is an inseparable part of general body health, is still widely ignored since adolescence. Maintaining the integrity of teeth is an important part of dental and oral health. Tooth loss can cause functional, aesthetic, and social problems, reduce a person's quality of life, and can be an effective indicator of community oral health. Purpose: Empowering and educating about dental and oral health for adolescents to increase their awareness of dental and oral health. Method: Dental and oral health counseling and dental health examinations were conducted on 50 adolescents, students of Madrasah Aliyah Miftahul Ulum, Melirang, Gresik. This study included quantitative and qualitative methods to assess changes in knowledge and perceptions about denture care among participants. The stages of implementation carried out in this study included pre-test, counseling, question and answer, post-test, dental and oral examination. Results: The examination results showed that out of 50 teenagers at MA Miftahul Ulum, Melirang, Gresik, 0 used dentures, 0 had caries + missing teeth, 0 had missing teeth, 3 had restorations, 5 had caries + remaining roots, 9 had remaining roots, 9 had caries, and 29 had no abnormalities. The results of the post-test questionnaire showed an increase in insight, compared to the pre-test. Conclusion: The conclusion of the activity showed that there was an increase in adolescents' knowledge and understanding of dental and oral health, as well as the importance of using dentures.

Title

Found 6 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 6 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 6 Documents
Search