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All Journal Dentika Dental Journal Dental Journal (Majalah Kedokteran Gigi) Indonesian Journal of Dental Medicine Berkala Penelitian Hayati Indonesian Journal of Prosthodontics

Utari Kresnoadi

Department Of Prosthodontic, Faculty Of Dental Medicine, Universitas Airlangga

Author-ID : 3197132

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

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Potency of Garcinia mangostana L peel extract combined with demineralized freeze-dried bovine bone xenograft on IL-1β expression, osteoblasts, and osteoclasts in alveolar bone Imam Safari Azhar; Utari Kresnoadi; Retno Pudji Rahayu
Dental Journal (Majalah Kedokteran Gigi) Vol. 50 No. 3 (2017): September 2017
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Alveolar bone plays an important role in supporting dentures. One socket preservation procedure is the application of bone graft to the tooth socket immediately after extraction. The administration of Garcinia mangostana L peel extract combined with demineralized freeze-dried bovine bone xenograft (DFDBBX) is assumed to decrease the number of osteoclast cells and IL-1β expressions, while also increasing that of osteoblast cells. Purpose: This study aimed to establish the potency of Garcinia mangostana L peel extract combined with DFDBBX on interleukin 1β expressions, osteoclast cells, and osteoblast cells during the preservation of tooth extraction sockets. Methods: This research constituted experimental laboratory-based research using 56 Cavia cobayas as specimens with randomized factorial design (true experimental design). These subjects were divided into eight groups, given PEG, DFDBBX, mangosteen peel extract or a combination of mangosteen peel extract and DFDBBX, and subsequently anesthesized before their left mandibular incisors were extracted. Thereafter, the post-extraction sockets were treated with PEG, DFDBBX, mangosteen peel extract or a combination of mangosteen peel extract and DFDBBX. The sockets were examined by means of HE and immunohistochemical staining on days 7 and 30 after extraction. The data obtained was analyzed with a one-way ANOVA test. Results: The results of the one-way ANOVA test showed that the average number of osteoblasts, osteoclasts, and IL-1β expressions varied significantly between the groups. Conclusion: The combination of mangosteen peel extract and DFDBBX can potentially increase osteoblasts, while also decreasing osteoclasts and IL-1β expressions in the alveolar bones of Cavia cobaya.

Effects of hydroxyapatite gypsum puger scaffold applied to rat alveolar bone sockets on osteoclasts, osteoblasts and the trabecular bone area Amiyatun Naini; I Ketut Sudiana; Mohammad Rubianto; Utari Kresnoadi; Faurier Dzar Eljabbar Latief
Dental Journal (Majalah Kedokteran Gigi) Vol. 52 No. 1 (2019): March 2019
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Damage to bone tissue resulting from tooth extraction will cause alveolar bone resorption. Therefore, a material for preserving alveolar sockets capable of maintaining bone is required. Hydroxyapatite Gypsum Puger (HAGP) is a bio-ceramic material that can be used as an alternative material for alveolar socket preservation. The porous and rough surface of HAGP renders it a good medium for osteoblast cells to penetrate and attach themselves to. In general, bone mass is regulated through a remodeling process consisting of two phases, namely; bone formation by osteoblasts and bone resorption by osteoclasts. Purpose: This research aims to identify the effects of HAGP scaffold application on the number of osteoblasts and osteoclasts, as well as on the width of trabecular bone area in the alveolar sockets of rats. Methods: This research used Posttest Only Control Group Design. There were three research groups, namely: a group with 2.5% HAGP scaffold, a group with 5% HAGP scaffold and a group with 10% HAGP scaffold. The number of samples in each group was six. HAGP scaffold at concentrations of 2.5%, 5% and 10% was then mixed with PEG (Polyethylene Glycol). The Wistar rats were anesthetized intra-muscularly with 100 mg/ml of ketamine and 20 mg/ml of xylazine base at a ratio of 1:1 with a dose of 0.08-0.2 ml/kgBB. Extraction of the left mandibular incisor was performed before 0.1 ml preservation of HAGP scaffold + PEG material was introduced into the extraction sockets and suturing was performed. 7 days after preparation of the rat bone tissue, an Hematoxilin Eosin staining process was conducted in order that observation under a microscope could be performed. Results: There were significant differences in both the number of osteoclasts and osteoblasts between the 2.5% HAGP group, the 5% HAGP group and the 10% HAGP group (p = 0.000). Similarly, significant differences in the width of the trabecular bone area existed between the 5% HAGP group and the 10% HAGP group, as well as between the 2.5% HAGP group and the 10% HAGP group (p=0.000). In contrast, there was no significant difference in the width of the trabecular bone area between the 2.5% HAGP group and the 5% HAGP group. Conclusion: The application of HAGP scaffold can reduce osteoclasts, increase osteoblasts and extend the trabecular area in the alveolar bone sockets of rats.

The effect of a combination of propolis extract and bovine bone graft on the quantity of fibroblasts, osteoblasts and osteoclasts in tooth extraction sockets Louisa Christy Lunardhi; Utari Kresnoadi; Bambang Agustono
Dental Journal (Majalah Kedokteran Gigi) Vol. 52 No. 3 (2019): September 2019
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v52.i3.p126-132

Background: Tooth extraction is a procedure frequently performed in the field of dentistry that can cause alveolar bone resorption during the healing process. Therefore, preservation of sockets is necessary to maintain alveolar bone which represents one of the important factors in the successful manufacture of dentures. A combination of propolis extract and bovine bone graft (BBG) can accelerate bone regeneration. Purpose: The purpose of this study was to determine the effects of a combination of propolis extract and BBG on the quantity of fibroblasts, osteoblasts, and osteoclasts in the tooth extraction socket. Methods: 56 Cavia cobaya were divided into eight groups. The lower left incisor of each subject was extracted and induced with polyethylene glycol (PEG), propolis extract + PEG, BBG + PEG, combination of propolis extract + BBG + PEG at a concentration of 2% active substance. Experimental subjects were sacrificed on days 3 and 7. Histopathological examination with a microscope at 400x magnification was conducted to calculate the quantity of fibroblasts, osteoblasts, and osteoclasts. Statistical analysis was performed by one-way ANOVA and Tukey HSD tests. Results: The highest average quantity of fibroblasts and osteoblasts and the lowest average quantity of osteoclasts occurred in the group to which a combination of propolis extract and BBG had been administered on both days 3 and 7. According to the statistical analysis results, all the treatment groups recorded a significant difference in the quantity of fibroblasts, osteoblasts, and osteoclasts with a p value: 0.000 (p<0.05). Conclusion: A combination of propolis extract and BBG can increase the quantity of fibroblast and osteoblast cells, while reducing the number of osteoclast cells in tooth extraction sockets treated with 2% concentration of the active substance.

The effect of propolis extract and bovine bone graft combination on the number of osteoclast and osteoblast as an effort to preserve post-extraction socket (on Cavia cobaya) Much Nizar; Utari Kresnoadi; S. Soekobagiono
Dental Journal (Majalah Kedokteran Gigi) Vol. 53 No. 1 (2020): March 2020
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v53.i1.p10-15

Background: Post-extraction alveolar bone height and width never reach the same dimensions as before extraction. A combination of propolis extracts and bovine bone graft (BBG) that are anti-inflammatory, antioxidant, osteoinductive and osteconductive is expected to improve bone regeneration. Purpose: Knowing the effect of the combination of propolis extracts and BBG on the number of osteoclast and osteoblast as an effort to preserve the socket after extraction of Cavia cobaya teeth. Methods: 56 Cavia cobaya was divided into eight groups: the lower left incisor was extracted, and the socket was filled with 2% poly ethylene glycol (PEG), propolis extract+PEG, BBG+PEG, and a combination of propolis extract+BBG+PEG. The incisors socket of animals models were executed on the 14th and 30th days. Using HE for histopathological examination, the number of osteoclasts and osteoblasts were counted with a 400x magnification light microscope with nine visual fields. The data were analysed via one-way ANOVA and Tukey HSD tests. Results: The highest mean number of osteoclasts occurred in the BBG+PEG 14th day group and the lowest occurred in the propolis extract+BBG+PEG 14th day group. The highest mean number of osteoblasts occurred in the propolis + BBG + PEG combination 30th day group, the lowest occurred in the control group (PEG) on the 14th day. Conclusion: The 2% combination of propolis extracts and BBG effectively reduces the osteoclast number and increases the osteoblast number in preserving the socket after extracting Cavia cobaya teeth.

Effective dose of propolis extract combined with bovine bone graft on the number of osteoblasts and osteoclasts in tooth extraction socket preservation Teguh Setio Yuli Prabowo; Utari Kresnoadi; Hanoem Eka Hidayati
Dental Journal (Majalah Kedokteran Gigi) Vol. 53 No. 1 (2020): March 2020
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v53.i1.p40-44

Background: Maintaining a good ridge is required during prosthodontic treatment. Hence, adequate alveolar bone support is considered an important factor in pursuing successful dentures. Propolis extract combined with bovine bone graft is a recent and innovative material in the process of socket preservation, as the caffeic acid phenethyl ester (CAPE) it contains can suppress the inflammatory process. Purpose: This study aims to determine the effective dose of propolis extract combined with bovine bone graft on the number of osteoblasts and osteoclasts in socket preservation. Methods: Twenty-eight Cavia cobaya animals were divided into four groups of seven. Group I was given 25 grams of PEG, while Group II were given a propolis extract at a dose of 0.5% combined with bovine bone graft. Group III were given a propolis extract at a dose of 1% combined with bovine bone graft and Group IV were given a propolis extract at a dose of 2% combined with bovine bone graft. On day 30, the lower incisor of each subject was extracted and induced with PEG and propolis (dose 0.5%, 1%, 2%). Histopathological examinations of osteoblasts and osteoclasts were measured with a 400x magnification light microscope. One-way ANOVA and Tukey HSD tests were performed to analyse data statistically. Results: The propolis extract combined with bovine bone graft not only increased the number of osteoblasts but also reduced the number of osteoclasts. The most effective dose for the propolis extract combined with bovine bone graft was 2%. Conclusion: The propolis extract combined with bovine bone graft could be effective in tooth extraction socket preservation at a dose of 2%.

Telescopic overdenture as an alternative rehabilitation for the loss of several anterior teeth due to traffic accidents Birgitta Dwitya Swastyayana Subiakto; Utari Kresnoadi
Dental Journal (Majalah Kedokteran Gigi) Vol. 53 No. 3 (2020): September 2020
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v53.i3.p126-132

Background: Telescopic overdenture is a prosthesis consisting of a primary coping, or an inner crown, that is attached to a supporting tooth in the oral cavity, and a secondary coping, or an outer crown, attached to a denture, which must be compatible with the primary coping. Purpose: The purpose of this study was to restore function and aesthetics and uplift the psychological status of the patient by fabrication of a fixed removable prosthesis using the existing abutment teeth as a telescopic overdenture. Case: A 36-year-old female came to the Prosthodontic RSGM UNAIR speciality clinic on her own volition to make front dentures for her upper and lower jaws after a traffic accident one and a half years ago. The patient had an arch bar installed two months after the accident, which was removed after two months. The patient wanted new dentures to improve both her ability to eat and her appearance. Case Management: Preliminary treatments performed were; maxillary and mandibular scaling and root planing; crown lengthening of tooth 15; extraction of teeth 16, 41, 42, and 43; and alveolectomy of the mandibular anterior region. Before the definitive treatment to improve the aesthetic appearance could be carried out, the first step was to make maxillary and mandibular transitional dentures. These were to be used while waiting for healing to occur, following the socket preservation in the mandibular area. Then, for the definitive restoration, we used telescopic overdenture for the maxilla and a removable partial denture for the mandible. Conclusion: Telescopic overdenture is recommended for patients who need good aesthetics for anterior tooth loss. Telescopic overdenture uses double crowns as the retentive elements, which give better aesthetic results compared to clasps, thereby improving the psychological status of the patient. In addition, they have better retention and stability compared to conventional complete dentures.

Tissue repair in post extraction sockets of Cavia cobaya induced by a combination of propolis and graft Utari Kresnoadi; Roselini Halim; Hananah Oktalidial Putri; Mutiara Aryanita; Imam Safari Azhar
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 25 No 2 (2020): June 2020
Publisher : The East Java Biological Society

Propolis, hive defensive substance that contains caffeic acid phenethyl ester, can affect the migration and proliferation of cells involved in the healing process. Combination of propolis extract with BBG are predicted to fasten the wound healing, especially its effect on angiogenesis, macrophages and collagen density. In this study, we aimed to analyze the effect of a propolis combination and BBG in proliferating new blood vesselsand macrophages, while also increasing the collagen density of the post-extracted tooth socket healing process in Cavia cobaya. 56 C. cobaya, whose left mandibular incisorshad been extracted, were subsequently divided into four groups on the basis of the substances used to fill their sockets, namely; control, BBG, propolis extract, and a combination of BBG, and propolis extract. The subjects sacrificed on either the third or seventh day. Histopathological samples were produced using HE and MT staining. The results were subjected tosome statistical analysis tests: one-way analysis of variance (ANOVA), Tukey’s honestly significant difference (HSD), Kruskal Wallis, and Mann Whitney. Significant increases (p<0.05) were detected between the PEG and PEG+Propolis groups and also between the PEG and PEG+BBG+Propolis combination groups. However, an insignificant increase (p>0.05) was identified between the PEG and BBG groups. In conclusion, a combination of BBG and propolis can increase the number of new blood vessels and macrophages together with the degree of collagen density in the healing process of post-extraction tooth sockets of C. cobaya.

THE INFLUENCE OF Aloe vera AND XENOGRAFT (XCB) TOWARD OF BONE MORPHO PROTEIN 2 ( BMP2) EXPRESSION AND AMOUNT OF OSTEOBLAST OF ALVEOLAR BONE INDUCED INTO TOOTH EXTRACTION SOCKETS (Cavia cobaya) Utari Kresnoadi; Retno Pudji Rahayu
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 19 No 2 (2014): June 2014
Publisher : The East Java Biological Society

Tooth extraction can cause inflammation leading to alveolar ridge resorption. In addition, prominent ridge has crucial role for making denture successfully. Thus, socket preservation is needed to prevent greater alveolar ridge resorption. An innovative material, a combination of Aloe vera and xenograft (XCB), is then considered as a biogenic stimulator that can reduce inflammation, as a result, the growth of alveolar bone is expected to be improved. This research is aimed to prove whether the mixture of Aloe vera and xenograft can stimulate BMP2 and increase osteoblasts. Forty-eight Cavia cobaya animals were divided into eight groups each of which consisted of six animals. The mandibular incisors of those Cavia cobaya animals were then extracted and filled with PEG as Group Control, XCB as Group XCB, Aloe vera as Group Aloe vera, and a combination of Aloe vera +XCB as Group Aloe vera +XCB. Next, the first four groups were sacrificed seven days after extraction, and the second four groups were sacrificed 30 days after extrac-tion. And then, immunohistochemical and histopathology examinations were conducted to examine BMP2 expression and osteoblasts. Based on the re-sult known that the mixture of Aloe vera and xenograft can increase BMP2 expression and amount of osteoblasts. It can be concluded that the mixture of Aloe vera and xenograft can increase BMP2 expression and amount of osteoblast cel . It can be used as an alternative material to increase the growth of alveolar bone after extraction.

Post-tooth extraction induction effect of Moringa oleifera leaf extract and demineralized freeze-dried bovine bone xenograft treatment on alveolar bone trabecula area Utari Kresnoadi; Najla Salsabila; Primanda Nur Rahmania; Phara Aster Chandra Adventia; Bima Subiakto Rahmani; Nobuhiro Yoda
Dental Journal Vol. 56 No. 2 (2023): June 2023
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v56.i2.p127-131

Background: After tooth extraction, alveolar bone resorption occurs naturally, followed by alveolar bone remodeling. Alveolar bone formation is characterized by an increase in density and expansion of the trabecular bone. Socket preservation using a combination of Moringa oleifera leaf extract and demineralized freeze-dried bovine bone xenograft (DFDBBX) is expected to increase the area of the alveolar bone trabeculae and thus accelerate the process of alveolar bone formation. Purpose: This study aimed to determine if a combination of Moringa oleifera leaf extract and DFDBBX could increase the area of the alveolar bone trabeculae in tooth extraction sockets. Methods: With their lower left incisors extracted, the 56 Cavia cobayas were divided into eight treatment groups according to the material given: polyethylene glycol (PEG), DFDBBX and PEG, Moringa oleifera leaf extract and PEG, and a combination of Moringa oleifera leaf extract, DFDBBX, and PEG. On the seventh and thirtieth days, the Cavia cobayas were sacrificed and examined. Histopathological samples were stained with Hematoxylin-Eosin (HE) to evaluate the trabecula area, and data were analyzed using one-way ANOVA and Tukey HSD. Results: On the thirtieth day, the group that received a combination of Moringa oleifera leaf extract and DFDBBX had the greatest area of alveolar bone trabeculae. Conclusion: A combination of Moringa oleifera leaf extract and DFDBBX induced in the tooth extraction socket can increase the area of the alveolar bone trabeculae.

Knowledge Improvement After Dental Health Education for Elementary School Students in Surabaya, East Java Sitalaksmi, Ratri Maya; Kresnoadi, Utari; Mundiratri, Karina; Utami, Farasty; Ashrin, Meinar Nur
Indonesian Journal of Dental Medicine Vol. 6 No. 1 (2023): Indonesian Journal of Dental Medicine
Publisher : Faculty of Dental Medicine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijdm.v6i1.2023.16-18

Background: During the coronavirus disease-19 (COVID-19) pandemic, many people were afraid to go to the dentist, which affected their dental and oral health conditions. Dental cavities may hamper children's growth and development; if this condition continues, it will affect the children's dietary intake, which may impact their quality of life and, if allowed to, cause stunting. Although in this condition, we still have to take preventive precautions with dental health education in school-age children. This required increasing dental health knowledge and understanding of dental disorders, particularly. Purpose: To describe oral and dental health knowledge improved following dental health education at Muhammadiyah 4 Elementary School, which is located in Pucang, Surabaya City, East Java. Methods: Two surveyors were responsible for data collecting. This study's sample size was 105 people ranging from third to fifth grade. The seminar covered dental and oral health via a hybrid method. The participants were asked to fill out a questionnaire form for the pre-and post-test to determine the improvement of oral and dental health knowledge for the dental health education evaluation. Results: Most oral and dental health participants understood the presentation on oral and dental health education. The post-test percentage was higher (89.14%) than the pre-test rate (60.29%). Conclusion: The program findings demonstrated that oral and dental health education with a hybrid method effectively increased elementary school children's understanding of dental and oral health status.

Co-Authors - UBSI Karawang, Djulaeha Adi Subianto Amiyatun Naini Anita Yuliati Arif Rachman Azhar, Imam Safari Bambang Agustono Bima Subiakto Rahmani Birgitta Dwitya Swastyayana Subiakto Faurier Dzar Eljabbar Latief Hananah Oktalidial Putri Hanoem Eka Hidayati Harly Prabowo, Harly Karina Mundiratri Louisa Christy Lunardhi Maretaningtias Dwi Ariani, Maretaningtias Dwi Meinar Nur Ashrin Michael Josef Kridanto Kamadjaja Mohammad Rubianto Much Nizar Muhammad Dimas Aditya Ari Mutiara Aryanita Najla Salsabila Nobuhiro Yoda Phara Aster Chandra Adventia Primanda Nur Rahmania Ratri Maya Sitalaksmi Retno Pudji Rahayu Roselini Halim Rostiny Rostiny, Rostiny S.Pd. M Kes I Ketut Sudiana . Soekobagiono , Teguh Setio Yuli Prabowo Utami, Farasty Waloejo Noegroho Yurike Hadisoesanto

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