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All Journal Animal Production : Indonesian Journal of Animal Production JMM (Jurnal Masyarakat Mandiri) Justek : Jurnal Sains Dan Teknologi Jurnal Teknologi Pertanian Jurnal Riset Agribisnis dan Peternakan Jurnal Ilmiah Fillia Cendekia Community Empowerment Jurnal Sains Peternakan Nusantara Agroindustrial Journal Kandang : Jurnal Peternakan Jurnal Ilmiah Ilmu-Ilmu Peternakan
Roisu Eny Mudawaroch
Fakultas Pertanian Universitas Muhammadiyah Purworejo
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Education Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Mathematics Public Health Social Sciences Veterinary Other

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Journal : Agroindustrial Journal

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Molecular Identification of Lactic Acid Bacteria from Broiler Chicken Meat Mudawaroch, Roisu Eny; Setiyono, Setiyono; Yusiati, Lies Mira; Suryanto, Edi
Agroindustrial Journal Vol 10, No 2 (2023)
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology UGM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/aij.v10i2.89982

Abstract

Lactic acid bacteria are bacteria used to ferment food. Molecular identification of isolates of lactic acid bacteria has a high degree of accuracy compared to morphological identification. This study aimed to molecularly identify isolates of lactic acid bacteria from broiler chickens. Molecular identification is carried out in 3 stages, namely: rRNA gene amplification stage, squencing and phylogeny tree. PCR electrophoresis results obtained amplicone results measuring about 1,500 bp. The results of BLAST analysis showed that the gen 16S rRNA sequence of BR 17 lactic acid bacteria isolate had a 97-98% similarity with the genome sequence of the Lactobacillus fermentum strain.  Development of phylogenetic tree isolates of single clusters of lactic acid bacteria BR 17 with strains of Lactobacillus fermentum CAU2036, Lactobacillus fermentum JCM 7772, and Lactobacillus fermentum 10-18. The identification results of lactic acid bacteria isolate BR 17 showed Lactobacillus fermentum BR 17.
Co-Authors Abadi, Jaya Adi Wibowo Aji, Bayu Laksono Aljundy, Muhammad Faqih Minded Aljundy Ariyanti, Atika Edi Suryanto Lies Mira Yusiati Lukman Fadhiliya, Lukman Mardhianto, Mardhianto Musholihah, Amam Najib, Muhammad Sufyan Nurfaizin, Nurfaizin Pamungkas, Sapto Rinawidiastuti, Rinawidiastuti Rizki Amelia Setiyono Setiyono Shintiani, Selly Sukma, Nuraeni Ayu Supriyatin, Tabah Tri, Indra Gaya Wibawanti, Jeki Mediantari Wahyu Zubair, Arif Zulfanita Zulfanita