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Antibacterial Activity Test of Elephant ginger (Zingiber officinale Rosc.) Endophytic Fungi Variation of Elephants Against Bacteria That Cause Skin Infections seniwati seniwati; Laras Aprilia; Rusli Rusli; Tadjuddin Naid
Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) Volume 13, No 2: December 2020
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ica.v13i2.10771

Abstract

Elephant ginger rhizome has the potential as an antibacterial, therefore the research was conducted to test the antibacterial activity of elephant ginger endophytic fungi against bacteria that cause skin infections, determine test bacteria that can be inhibited by elephant ginger endophytic fungi and bioautogram profile for its antibacterial activity. Isolation of endophytic ginger elephant fungi using the direct planting method. Screening of endophytic fungi isolates was placed on the surface of NA medium containing test bacteria Staphylococcus aureus, Staphylococcus epidermidis, and Propionibacterium acne. The screening results obtained were 2 isolates namely isolates which had the highest activity namely IFDRJG03 and IFDRJG04. For IFDRJG03 isolates can inhibit the antibacterial activity of Staphylococcus aureus 25.26 mm, Staphylococcus epidermidis 26.89 mm and Propionibacterium acne 27.12 mm, IFDRJG04 isolates can inhibit the antibacterial activity of Staphylococcus aureus 15.87 mm, Staphylococcus epidisidis 18 and 96 mm. 27.43 mm. Then the TLC-Bioautography test was carried out, the results of IFDRJG03 isolates were obtained using chloroform eluents: methanol (1: 1) had antibacterial activity against Staphylococcus aureus with Rf1 = 0.9 and Rf2 = 0.5, Staphylococcus epidermidis with Rf1 value = 0.8 and Rf2 = 0.5 and Propionibacterium acne with Rf1 value = 0.9 and Rf2 = 0.5 and isolation of IFDRJG04 isolates with ethyl acetate eluent: ethanol: water (8: 2: 1) has antibacterial activity against Staphylococcus aureus Rf1 values = 0.9 Rf2 = 0.7 Rf3 = 0.6, Staphylococcus epidermidis with Rf1 value = 0.7 Rf2 = 0.6, Rf3 = 0.5 and Propionibacterium acne with Rf1 value = 0.9 and Rf2 = 0.7.lephant ginger rhizome has the potential as an antibacterial, therefore the research was conducted to test the antibacterial activity of elephant ginger endophytic fungi against bacteria that cause skin infections, determine test bacteria that can be inhibited by elephant ginger endophytic fungi and bioautogram profile for its antibacterial activity. Isolation of endophytic ginger elephant fungi using the direct planting method. Screening of endophytic fungi isolates was placed on the surface of NA medium containing test bacteria Staphylococcus aureus, Staphylococcus epidermidis, and Propionibacterium acne. The screening results obtained were 2 isolates namely isolates which had the highest activity namely IFDRJG03 and IFDRJG04. For IFDRJG03 isolates can inhibit the antibacterial activity of Staphylococcus aureus 25.26 mm, Staphylococcus epidermidis 26.89 mm and Propionibacterium acne 27.12 mm, IFDRJG04 isolates can inhibit the antibacterial activity of Staphylococcus aureus 15.87 mm, Staphylococcus epidisidis 18 and 96 mm. 27.43 mm. Then the TLC-Bioautography test was carried out, the results of IFDRJG03 isolates were obtained using chloroform eluents: methanol (1: 1) had antibacterial activity against Staphylococcus aureus with Rf1 = 0.9 and Rf2 = 0.5, Staphylococcus epidermidis with Rf1 value = 0.8 and Rf2 = 0.5 and Propionibacterium acne with Rf1 value = 0.9 and Rf2 = 0.5 and isolation of IFDRJG04 isolates with ethyl acetate eluent: ethanol: water (8: 2: 1) has antibacterial activity against Staphylococcus aureus Rf1 values = 0.9 Rf2 = 0.7 Rf3 = 0.6, Staphylococcus epidermidis with Rf1 value = 0.7 Rf2 = 0.6, Rf3 = 0.5 and Propionibacterium acne with Rf1 value = 0.9 and Rf2 = 0.7.
Obesitas dan Obesitas Sentral pada Masyarakat Usia Dewasa di Daerah Perkotaan Indonesia Septiyanti Septiyanti; Seniwati Seniwati
Jurnal Ilmiah Kesehatan (JIKA) Vol. 2 No. 3 (2020): Volume 2 Nomor 3 Desember 2020
Publisher : Sarana Ilmu Indonesia (salnesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (485.025 KB) | DOI: 10.36590/jika.v2i3.74

Abstract

Obesity is a problem in various parts of the world where its prevalence is increasing rapidly, both in developed and developing countries Obesity can occur because of an imbalance between the energy from the food that comes in, which is greater than the energy used by the body. This study aimed to see the characteristics of obesity and central obesity in adult society in urban areas of Indonesia. This study used a cross-sectional study, by analyzing advanced data on Basic Health Research (Riskesdas) in the biomedical field. The results of this study indicated that in general obesity and central obesity increase with age, with the highest prevalence being at the age of 40-59 years. Both obesity and central obesity were more prevalent in female subjects. Obesity and central obesity were also more common among subjects who graduated from high school and who work as housewives. Subjects of obesity and central obesity experienced more abnormalities in biomedical examination than those with normal BMI and abdominal circumference. For this reason, it is advisable to maintain a normal BMI and abdominal circumference, especially for adults. This study proves that there are significant differences in biomedical examination in those who are obese and not obese.