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Kandungan flavanoid total dan aktivitas antimikroba serbuk biji kakao (Theobroma cacao) asal kabupaten enrekang Sulawesi Selatan Emelda, Andi; Rusli, Rusli; Nurlina, Nurlina; Santi, Irma
Pharmaciana Vol 6, No 2 (2016): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Cocoa and its derivatives are rich in flavonoids. Flavonoid content of cocoa beans is affected by the treatment process. The aim of this study are to determine total flavonoids and antimicrobial activity of cocoa powder. This study used unfermented cocoa beans in the form of powder. Testing of the total flavonoids was conducted using an aluminum chloride colorimetric assay. The minimum inhibitory concentration (MIC), Minimum Bactericidal Concentration (MBC), and antimicrobial activity of cocoa bean powder were tested by using ten bacteria: Staphylococcus epidermidis, Bacillus subtilis, Escherichia coli, Salmonella thypi, Shigella dysentriae, Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus mutans, Vibrio cholerae, and Propioni bacterium agne. The result of measurement of the total flavonoid of unfermented cocoa powder is 22.62 mg EC/g. The screening of antimicrobial with concentration of 0.1% of cocoa powder gives inhibition in Streptococcus mutans, dysentriae Shigella, Escherichia coli, Vibrio cholerae bacterium and Propioni bacterium agne, while at a concentration of 0.5% gives inhibition at all test bacteria. In the antimicrobial activity test using a concentration of 0.5%, 1%, 2% and 4%, the highest resistance zone is shown at a concentration of 4 %. The results show that the diameter zone of inhibition for the bacteria Shigella dysentriae is 11.33 mm. The 10 mm of diameter zone of inhibition are indicated by streptococcus mutans, Escherichia coli, Propioni bacterium agne, Staphylococcus aureus and Bacillus subtilis while 11 mm zone of inhibition demonstrated by Salmonella thypi and Staphylococcus epidermidis. The number of the diameter zone of inhibition, 12 mm, was shown in Pseudomonas aeruginosa. In conclusion, the powder of unfermented cocoa beans provides flavonoids 22,62 mg EK/g and have the antimicrobial activity.

OPTIMASI PRODUKSI ANTIBIOTIKA ISOLAT TERPILIH FUNGI ENDOFIT IKD FF-UMI 02 DARI KULIT BUAH DELIMA (Punica granatum L.) DENGAN VARIASI SUMBER KARBON Rusli Rusli; Nurul Vicky Syfriani; Sumarni Hatta; Muh. Wais
As-Syifaa Jurnal Farmasi Vol 9, No 1 (2017): AS-SYIFAA Jurnal Farmasi
Publisher : Fakultas Farmasi UMI

Research of endophytic fungi of pomegranate fruit peel (Punica granatum L.), as Anti-free radicals and antibiotic has been done with DIKTI-PKM funding at 2014. It is considered necessary to further research about optimization of antibiotic production from pomegranate fruit peel with variation of carbon source, first step is selection of isolates that have largest inhibition zone and has the most inhibit microbial test, that is endophytic fungi IKD FF-UMI 02 that has activity on Salmonella thypii, Staphylococcus epidermidis, Staphylococcus aureus and Shigella dysenteriae. Determination of growth curve from isolate by inoculating into Potato Dextrose Broth and incubated for 7x24 hours. Every 12 hours the mycelium is weighed to determine its stationary phase. Determination of optimum conditions of fermentation with carbon source such as glucose, galactose, and fructose. Endophytic fungi IKD FF-UMI 02 inoculated and fermented into production medium which is every 12 hours fermentate was taken. The activity was tested using agar diffusion method in Nutrient Agar (NA) against Salmonella thypii, Staphylococcus epidermidis, Staphylococcus aureus and Shigella dysenteriae. Resulted that growth curve of selected endophytic fungi IKD FF-UMI 02 from pomegranate fruit peel show that stationer phase starting on 192 hour and galactose carbon source is show optimum time of antibiotic production on 120 hours with inhibition zone 10,24 mm against Shigella dysenteriae.

PENELUSURAN POTENSI MIKROBA ENDOFIT DARI RIMPANG PAKU KEPALA TUPAI (Drynaria quercifolia J.Smith) SEBAGAI PENGHASIL SENYAWA ANTIBIOTIKA Rusli Rusli; Dian Rahmaniar
As-Syifaa Jurnal Farmasi Vol 5, No 2 (2013): AS-SYIFAA Jurnal Farmasi
Publisher : Fakultas Farmasi UMI

This research object to obtain abd determine the activity of antibiotics microbial isolates from endophyticpakukepalatupai (Drynariaquercifolia J. Smith) as a producer of antibiotic compounds. Methods research is using the method in a medium cast nutrient agar and potato dextrose agar with a dilution of 10-1 to 10-5. Result showed that there were 5 active isolates on thosetwo medium, identification of isolates bacterial and fungi. Then, the bacteria and fungi isolates purified by quadrant method on nutrient agar and potato dextrose agar medium were in mikroskopic. To produce antibiotic compound fermentation carried out by maltose yeast broth. The results of testing the activity of an antibiotic fermentation with agar diffusion method, results showed that the bacterial active against Bacillus subtilis, Pseudomonas aeruginosa, Streptococcus mutans, Escherichia coli and fungal isolates aktifagains candida albicans. Identification of endophyte microbe isolates carried out by gram staining. Result obtained showed that all isolates of the bacteria were negative Gram and coccus and bacil from.Key word : Antibiotic, Microbial Endophyte, Drynaria quercifolia J. Smith

Kandungan flavanoid total dan aktivitas antimikroba serbuk biji kakao (Theobroma cacao) asal kabupaten enrekang Sulawesi Selatan Andi Emelda; Rusli Rusli; Nurlina Nurlina; Irma Santi
Pharmaciana Vol 6, No 2 (2016): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Cocoa and its derivatives are rich in flavonoids. Flavonoid content of cocoa beans is affected by the treatment process. The aim of this study are to determine total flavonoids and antimicrobial activity of cocoa powder. This study used unfermented cocoa beans in the form of powder. Testing of the total flavonoids was conducted using an aluminum chloride colorimetric assay. The minimum inhibitory concentration (MIC), Minimum Bactericidal Concentration (MBC), and antimicrobial activity of cocoa bean powder were tested by using ten bacteria: Staphylococcus epidermidis, Bacillus subtilis, Escherichia coli, Salmonella thypi, Shigella dysentriae, Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus mutans, Vibrio cholerae, and Propioni bacterium agne. The result of measurement of the total flavonoid of unfermented cocoa powder is 22.62 mg EC/g. The screening of antimicrobial with concentration of 0.1% of cocoa powder gives inhibition in Streptococcus mutans, dysentriae Shigella, Escherichia coli, Vibrio cholerae bacterium and Propioni bacterium agne, while at a concentration of 0.5% gives inhibition at all test bacteria. In the antimicrobial activity test using a concentration of 0.5%, 1%, 2% and 4%, the highest resistance zone is shown at a concentration of 4 %. The results show that the diameter zone of inhibition for the bacteria Shigella dysentriae is 11.33 mm. The 10 mm of diameter zone of inhibition are indicated by streptococcus mutans, Escherichia coli, Propioni bacterium agne, Staphylococcus aureus and Bacillus subtilis while 11 mm zone of inhibition demonstrated by Salmonella thypi and Staphylococcus epidermidis. The number of the diameter zone of inhibition, 12 mm, was shown in Pseudomonas aeruginosa. In conclusion, the powder of unfermented cocoa beans provides flavonoids 22,62 mg EK/g and have the antimicrobial activity.

Antibacterial Activity Test of Elephant ginger (Zingiber officinale Rosc.) Endophytic Fungi Variation of Elephants Against Bacteria That Cause Skin Infections seniwati seniwati; Laras Aprilia; Rusli Rusli; Tadjuddin Naid
Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) Volume 13, No 2: December 2020
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ica.v13i2.10771

Elephant ginger rhizome has the potential as an antibacterial, therefore the research was conducted to test the antibacterial activity of elephant ginger endophytic fungi against bacteria that cause skin infections, determine test bacteria that can be inhibited by elephant ginger endophytic fungi and bioautogram profile for its antibacterial activity. Isolation of endophytic ginger elephant fungi using the direct planting method. Screening of endophytic fungi isolates was placed on the surface of NA medium containing test bacteria Staphylococcus aureus, Staphylococcus epidermidis, and Propionibacterium acne. The screening results obtained were 2 isolates namely isolates which had the highest activity namely IFDRJG03 and IFDRJG04. For IFDRJG03 isolates can inhibit the antibacterial activity of Staphylococcus aureus 25.26 mm, Staphylococcus epidermidis 26.89 mm and Propionibacterium acne 27.12 mm, IFDRJG04 isolates can inhibit the antibacterial activity of Staphylococcus aureus 15.87 mm, Staphylococcus epidisidis 18 and 96 mm. 27.43 mm. Then the TLC-Bioautography test was carried out, the results of IFDRJG03 isolates were obtained using chloroform eluents: methanol (1: 1) had antibacterial activity against Staphylococcus aureus with Rf1 = 0.9 and Rf2 = 0.5, Staphylococcus epidermidis with Rf1 value = 0.8 and Rf2 = 0.5 and Propionibacterium acne with Rf1 value = 0.9 and Rf2 = 0.5 and isolation of IFDRJG04 isolates with ethyl acetate eluent: ethanol: water (8: 2: 1) has antibacterial activity against Staphylococcus aureus Rf1 values = 0.9 Rf2 = 0.7 Rf3 = 0.6, Staphylococcus epidermidis with Rf1 value = 0.7 Rf2 = 0.6, Rf3 = 0.5 and Propionibacterium acne with Rf1 value = 0.9 and Rf2 = 0.7.lephant ginger rhizome has the potential as an antibacterial, therefore the research was conducted to test the antibacterial activity of elephant ginger endophytic fungi against bacteria that cause skin infections, determine test bacteria that can be inhibited by elephant ginger endophytic fungi and bioautogram profile for its antibacterial activity. Isolation of endophytic ginger elephant fungi using the direct planting method. Screening of endophytic fungi isolates was placed on the surface of NA medium containing test bacteria Staphylococcus aureus, Staphylococcus epidermidis, and Propionibacterium acne. The screening results obtained were 2 isolates namely isolates which had the highest activity namely IFDRJG03 and IFDRJG04. For IFDRJG03 isolates can inhibit the antibacterial activity of Staphylococcus aureus 25.26 mm, Staphylococcus epidermidis 26.89 mm and Propionibacterium acne 27.12 mm, IFDRJG04 isolates can inhibit the antibacterial activity of Staphylococcus aureus 15.87 mm, Staphylococcus epidisidis 18 and 96 mm. 27.43 mm. Then the TLC-Bioautography test was carried out, the results of IFDRJG03 isolates were obtained using chloroform eluents: methanol (1: 1) had antibacterial activity against Staphylococcus aureus with Rf1 = 0.9 and Rf2 = 0.5, Staphylococcus epidermidis with Rf1 value = 0.8 and Rf2 = 0.5 and Propionibacterium acne with Rf1 value = 0.9 and Rf2 = 0.5 and isolation of IFDRJG04 isolates with ethyl acetate eluent: ethanol: water (8: 2: 1) has antibacterial activity against Staphylococcus aureus Rf1 values = 0.9 Rf2 = 0.7 Rf3 = 0.6, Staphylococcus epidermidis with Rf1 value = 0.7 Rf2 = 0.6, Rf3 = 0.5 and Propionibacterium acne with Rf1 value = 0.9 and Rf2 = 0.7.

Anti-free radical activity test of endophytic fungal fermentate extract on the Snake Plants (Sansevieria trifasciata Hort. Ex Prain) using the TLC-Autography method Seniwati; Rusli; Adelia Fitrah; Tadjuddin Naid
Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) Volume 14, No 2: December 2021
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ica.v14i3.14496

Degenerative diseases occur due to damage to cells, fat tissue, and the immune system caused by several factors. One theory that causes degenerative diseases is the presence of free radicals. The Snake plant is one of the plants in Indonesia that has long been known as a plant that is useful as anti-free radicals. The research was conducted by isolating and purifying endophytic fungi and determining the autogram profile of free antiradical activity from The Snake plant. From the purification results obtained 7 isolates, followed by macroscopic examination and fermentation of each isolate. The results of the screening test showed that 2 fermentates were active as anti-free radicals, namely AF 1 and AF 3. Fermentate isolates AF 1 and AF 3 were identified by TLC-autography using a mixture of n-hexane: ethyl acetate (7:1). TLC-autography test results by spraying DPPH isolates AF 1 and isolates AF 3 showed yellow spots on a purple background with Rf1 values of 0.90 and Rf2 of 0.2 in AF1 isolates and Rf1 values of 0.83 and Rf2 of 0.16 on AF3 isolates. potential as anti-free radicals. From the results of this study, it was concluded that there was anti-free radical activity in the Snake plant.

ISOLASI DAN IDENTIFIKASI SENYAWA ANTIBAKTERI EKSTRAK N- HEKSAN DAUN JATI (Tectona grandis L.F) Andi Armisman Edy Paturusi; Nurafianty Nurafianty; Rusli Rusli; Abdul Rahim
Jurnal Farmasi UIN Alauddin Makassar Vol 2 No 1 (2014): Jurnal Farmasi
Publisher : Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/jurfar.v2i1.2151

Research about Isolation and identification of jati leave extract have been done. The purpose of this research is to isolate and identify the antibacterial compound of jati leave. The sample was used in this research obtained from Pharmacognocy-Phytochemistry Laboratory Islamic State University of Alauddin Makassar collection. These sample have antibacterial activity to Bacillus subtilis, Pseudomonas aeruginosa, Salmonella typhosa and Staphylococcus epidermidis when conducted by Bioautography Thin Layer Chromatography (TLC). Second fraction was isolated by Preparative Thin Layer Chromatography (PTLC) and result 2 bands. The second isolat tested by Bioautography Thin Layer Chromatography (TLC) and showing antibacterial activity to Bacillus subtilis, Pseudomonas aeruginosa and Salmonella typhosa and it was single isolat when treat by multi-eluen TLC and two dimension. The next step identify the second isolat by spot reagen and showing positif reaction steroid compound. Ultraviolet spectroscopy interpretation have maximum wavelength 242 nm and infrared spectroscopy showing -OH, -CH alifatic, -CO, -CH3, alkanes, alkenes and -C=C.

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