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All Journal Padjadjaran Journal of Dentistry

S. Supriatno

Universitas Gadjah Mada

Author-ID : 3240570

Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Articles

Electro-gene therapy followed by intratumoral injection of pcDNA3.1-p27Kip1 wild type in human tongue base cancer cells SP-C3 xenograft S. Supriatno; Inne Suherna Sasmita
Padjadjaran Journal of Dentistry Vol 21, No 3 (2009): November 2009
Publisher : Faculty of Dentistry Universitas Padjadjaran

Human tongue base cancers are characterized by a high degree of local invasion and metastasis to the regional lymph nodes and included a disease with difficult treatment. A novel method for high-efficiency and region-controlled in vivo gene transfer was developed by combining electro-gene therapy and plasmid (pcDNA). The aims of the study were to examine the efficiency of transfection of p27Kip1 gene by electro-gene therapy and to evaluate p27Kip1 gene therapy in Supri’s clone-3 (SP-C3) xenografts using pcDNA3.1-p27Kip1 wild-type (wt) and pcDNA3.1 empty vector (neo) with electro-gene therapy. To investigate gene transfer method, the enhanced green fluorescence protein (EGFP) gene was transfected into xenografts by electro-gene therapy. The efficiency of p27Kip1 gene transfection at protein level was confirmed by Western blotting. To estimate the reduction in tumour size in Wistar Balb/c mice after electro-gene therapy with p27Kip1 wt gene was examined by tumorigenesis assay. To evaluate the induction of apoptosis was carried out by colourimetric assay. The result, the growth of tumours was markedly suppressed by p27Kip1 wt gene transfection. Up-regulation of p27Kip1 protein was detected in pcDNA3.1-p27Kip1 wt. Apoptosis induction through the activity of caspase -3 and -9 was significantly increased in p27Kip1 wt-transfected tumours. These results suggest that it is possible to transfer p27Kip1 wt into tongue base cancer cell xenografts using electro-gene therapy. p27Kip1 wt had a high-potential to suppress the growth of tumours. Conclusion, electro-gene therapy followed by intratumoral injection of pcDNA3.1-p27Kip1 wt had a high-potential to suppress the growth of a human tongue base cancer cell xenograft.

The effects of ethyl acetate fraction of Ananas Comosus (L.) Merr. of tongue cancer cell growth inhibition Supri’s Clone-1, invitro Maureen Martina; Roosje Rosita Oewen; Eriska Riyanti; Achmad Syawqie; S. Supriatno
Padjadjaran Journal of Dentistry Vol 23, No 2 (2011): July 2011
Publisher : Faculty of Dentistry Universitas Padjadjaran

Ananas comosus (L.) Merr has several purposes which include antioxidant and anti-inflammatory activity that shows some pharmacological effects and the subject of anti-cancer or anti-cancer supporting material. The research objective was to analyze the effects of ethyl acetate fraction of Ananas comosus (L.) Merr. of tongue cancer cell growth inhibition Supri’s clone-1 (SP-C1). This type of study was a research laboratory. Next, cell growth inhibition testing by the ethyl acetate fraction of Ananas comosus (L.) Merr. with various concentrations (0; 62.5; 125; 250; 250; 500 and 1000 microgram/ml) using the MTT assay test. Growth barriers identified by Biorad microplate tool reader with a wavelength of 540 nm. The number of SP-C1 cells examined was 2 x 104 cells/wells with incubation time 24 and 48 hours. Data were analyzed using a two-ways ANOVA followed by post hoc test (LSD test) with 95% significance level. The results showed ethyl acetate fraction of Ananas comosus (L.) Merr. able to inhibit the growth of cancer cells SP-C1. Various concentrations of ethyl acetate fraction of pineapple were highly significant, meaning that the concentration effect on cell growth of SP-C1. Similarly, incubation time effect on the growth of SP-C1 cells that were very meaningful. The biggest obstacle effect of ethyl acetate fraction of Ananas comosus (L.) Merr. occurred at a concentration of 1000 ug/ml (43.45%) with an incubation time of 48 hours. Conclusion of this study was the fraction of ethyl acetate Ananas comosus (L.) Merr. has the effect of inhibiting the growth of cancer cells SP-C1.

Ethanol extract of mangosteen (Garcinia Mangostana Linn) peel effect in inhibiting the growth of human tongue cancer cells Supri’s Clone 1, invitro Edi Suanto; Roosje Rosita Oewen; Inne Suherna Sasmita; S. Supriatno; Unang Supratman
Padjadjaran Journal of Dentistry Vol 23, No 2 (2011): July 2011
Publisher : Faculty of Dentistry Universitas Padjadjaran

The incidence of tongue cancer in Indonesia reached 1.01% of all cancers and 42% of oral cavity cancer. Tongue cancer therapies including chemotherapy, radiotherapy, surgery, and all three combined therapy. Search for anti-cancer drugs currently switched on herbal plants, one of which is the mangosteen. Has the properties of mangosteen peel extract inhibited the growth of cancer cells. The purpose of the study, obtain IC50 of ethanol extract of mangosteen peel in inhibiting the growth of human tongue cancer cells SP-C1. Research carried out on 96 preparations of human tongue cancer SP-C1 were incubated with ethanol extract of mangosteen peel, preparations were classified in two groups of incubation time (24 hours and 48 hours) and each group will be given preferential treatment over 6 randomly different concentrations: 0 (control), 62.5 μg/mL, 125 μg/mL, 250 μg/mL, 500 μg/mL and 1000 μg/mL. Model experiments were 2 x 6 factorial experiment with eight replication for each cell. Test results with ANAVA, incubation (24 and 48 hour) SP-tongue cancer cells with various concentrations of C1 ethanol extract of mangosteen peel gives a highly significant, indicating differences cancer cell growth inhibition. Incubation time factor showed the long incubation effect on cancer cell growth inhibition. Furthermore, by Newman Keuls test, showed 500μg/mL concentrations of 24-hour incubation had the best effect. Conclusion of the study of ethanol extract of mangosteen peel could achieve with IC50 values of cell growth resistance 50.3% at a concentration of 500 μg/mL and an incubation time of 24 hours.

Anti-tumor agent celecoxib activity towards SP-C1 tongue cancer cells invasion (in vitro) Harun Achmad; Mieke Hemiawati Satari; Roosje Rosita Oewen; S. Supriatno
Padjadjaran Journal of Dentistry Vol 23, No 1 (2011): March 2011
Publisher : Faculty of Dentistry Universitas Padjadjaran

Invasion is a characteristic of the occurrence of cancer and indicates the cancer cells' capability to destroy and degrade the border between the epithet and basal membrane to further spread into the surrounding extra-cellular matrix. The purpose of this research was to find the existence of impediment at the SP-C1 tongue cancer cell using celecoxib chemopreventive medication. The SP-C1 tongue cancer cells were treated in vitro using celecoxib medication as a research subject at the following concentrations 5, 10, 25, 50, 75, 100, 125%; and 0 as control group (only DMEM growth medium treatment). Pure experimental testing was carried out for 24 and 48 hours, with observation and calculation of an average number of SP-C1 tongue cancer cells. The data collected were analyzed using the ANOVA test with Newman Keuls paired range test or t-test. Research results indicated that the average number of SP-C1 tongue cancer cells invasion after administration of celecoxib medication based on administration concentration and time statistically yielded significant results. The ANOVA test results were statistically significant, that is, average occurrence of the number of SP-C1 tongue cancer cells due to the use of celecoxib at certain concentrations compared to that without celecoxib was different. At celecoxib of zero (control) concentration was 24.4 with celecoxib concentration starting at 5 up to 125% experienced a decline from its average 11 to become 2.3. The conclusion of the research was that the greater the celecoxib concentration administered, the greater the effect on the impediment of SP-C1 tongue cancer cell invasion.

Co-Authors Achmad Syawqie Edi Suanto Eriska Riyanti Harun Achmad Inne Suherna Sasmita Maureen Martina Mieke Hemiawati Satari Roosje Rosita Oewen Unang Supratman

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