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All Journal Padjadjaran Journal of Dentistry
Roosje Rosita Oewen
Universitas Padjadjaran
Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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The effects of ethyl acetate fraction of Ananas Comosus (L.) Merr. of tongue cancer cell growth inhibition Supri’s Clone-1, invitro Maureen Martina; Roosje Rosita Oewen; Eriska Riyanti; Achmad Syawqie; S. Supriatno
Padjadjaran Journal of Dentistry Vol 23, No 2 (2011): July 2011
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (237.853 KB) | DOI: 10.24198/pjd.vol23no2.14017

Abstract

Ananas comosus (L.) Merr has several purposes which include antioxidant and anti-inflammatory activity that shows some pharmacological effects and the subject of anti-cancer or anti-cancer supporting material. The research objective was to analyze the effects of ethyl acetate fraction of Ananas comosus (L.) Merr. of tongue cancer cell growth inhibition Supri’s clone-1 (SP-C1). This type of study was a research laboratory. Next, cell growth inhibition testing by the ethyl acetate fraction of Ananas comosus (L.) Merr. with various concentrations (0; 62.5; 125; 250; 250; 500 and 1000 microgram/ml) using the MTT assay test. Growth barriers identified by Biorad microplate tool reader with a wavelength of 540 nm. The number of SP-C1 cells examined was 2 x 104 cells/wells with incubation time 24 and 48 hours. Data were analyzed using a two-ways ANOVA followed by post hoc test (LSD test) with 95% significance level. The results showed ethyl acetate fraction of Ananas comosus (L.) Merr. able to inhibit the growth of cancer cells SP-C1. Various concentrations of ethyl acetate fraction of pineapple were highly significant, meaning that the concentration effect on cell growth of SP-C1. Similarly, incubation time effect on the growth of SP-C1 cells that were very meaningful. The biggest obstacle effect of ethyl acetate fraction of Ananas comosus (L.) Merr. occurred at a concentration of 1000 ug/ml (43.45%) with an incubation time of 48 hours. Conclusion of this study was the fraction of ethyl acetate Ananas comosus (L.) Merr. has the effect of inhibiting the growth of cancer cells SP-C1.
Anti-tumor agent celecoxib activity towards SP-C1 tongue cancer cells invasion (in vitro) Harun Achmad; Mieke Hemiawati Satari; Roosje Rosita Oewen; S. Supriatno
Padjadjaran Journal of Dentistry Vol 23, No 1 (2011): March 2011
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1465.155 KB) | DOI: 10.24198/pjd.vol23no1.14053

Abstract

Invasion is a characteristic of the occurrence of cancer and indicates the cancer cells' capability to destroy and degrade the border between the epithet and basal membrane to further spread into the surrounding extra-cellular matrix. The purpose of this research was to find the existence of impediment at the SP-C1 tongue cancer cell using celecoxib chemopreventive medication. The SP-C1 tongue cancer cells were treated in vitro using celecoxib medication as a research subject at the following concentrations 5, 10, 25, 50, 75, 100, 125%; and 0 as control group (only DMEM growth medium treatment). Pure experimental testing was carried out for 24 and 48 hours, with observation and calculation of an average number of SP-C1 tongue cancer cells. The data collected were analyzed using the ANOVA test with Newman Keuls paired range test or t-test. Research results indicated that the average number of SP-C1 tongue cancer cells invasion after administration of celecoxib medication based on administration concentration and time statistically yielded significant results. The ANOVA test results were statistically significant, that is, average occurrence of the number of SP-C1 tongue cancer cells due to the use of celecoxib at certain concentrations compared to that without celecoxib was different. At celecoxib of zero (control) concentration was 24.4 with celecoxib concentration starting at 5 up to 125% experienced a decline from its average 11 to become 2.3. The conclusion of the research was that the greater the celecoxib concentration administered, the greater the effect on the impediment of SP-C1 tongue cancer cell invasion.
Co-Authors Achmad Syawqie Eriska Riyanti Harun Achmad Maureen Martina Mieke Hemiawati Satari S. Supriatno