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Pola dan terapi infeksi Herpes simpleks virus-1 pada rongga mulut di RSUP Dr. Hasan Sadikin Bandung periode 2013-2017The pattern and therapy of the Herpes simplex virus-1 infection in the oral cavity at Dr Hasan Sadikin Hospital Bandung in 2013-2017 Farhani Azizah; Irna Sufiawati; Mieke Hemiawati Satari
Padjadjaran Journal of Dental Researchers and Students Vol 3, No 1 (2019): Februari 2019
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjdrs.v3i1.22501

Pendahuluan: Salah satu virus yang menginfeksi rongga mulut adalah Herpes Simpleks Virus-1 (HSV-1). Virus ini menjadi patogen utama pada berbagai macam inang dan dapat menyebabkan berbagai macam penyakit orofasial. Tatalaksana infeksi HSV-1 memiliki pola terapi yang beragam bergantung pada kondisi klinis pasien. Tujuan penelitian ini adalah memperoleh data mengenai pola dan terapi penyakit mulut karena infeksi HSV-1. Metode: Data yang dikumpulkan merupakan data sekunder berasal dari logbook dan rekam medik pasien di Poliklinik Gigi dan Mulut RSUP Dr. Hasan Sadikin Bandung periode 2013-2017. Sampel penelitian ditentukan menggunakan metode purposive sampling, yaitu rekam medik pasien dengan diagnosis penyakit mulut karena infeksi HSV-1. Hasil: Pola penyakit mulut pada instalasi rawat jalan yaitu Recurrent Intraoral Herpes (RIH) sebanyak 84,91%, Herpes Associated Erythema Multiforme (HAEM) 9,43%, Herpes labialis 3,77%, dan Primary Herpetic Gingivo Stomatitis (PHGS) 1,89%. Pola penyakit mulut pada rawat inap yaitu Recurrent Intraoral Herpes (RIH) sebanyak 85,71% dan Herpes labialis sebanyak 14,29%. Pemberian terapi sangat bervariatif yaitu kombinasi asiklovir, antiseptik, multivitamin, antiinflamasi steroid, NSAID, pelembab bibir, antibiotik, antihistamin, dan antifungal. Simpulan: Penyakit mulut karena infeksi HSV-1 yang paling sering ditemukan baik pada instalasi rawat jalan maupun rawat inap adalah recurrent intraoral herpes. Pemberian terapi yang paling sering digunakan pada instalasi rawat jalan yaitu kombinasi obat antiinflamasi steroid dan multivitamin, sedangkan pada instalasi rawat inap yaitu multivitamin dan kombinasi asiklovir, antiseptik, dan multivitamin.Kata kunci: Pola penyakit mulut, pola terapi, infeksi Herpes Simpleks Virus-1 ABSTRACTIntroduction: One of the viruses that infect the oral cavity is Herpes Simplex Virus-1 (HSV-1). This virus becomes the primary pathogen in various types of hosts and can cause various kinds of orofacial diseases. Management of HSV-1 infection has a diverse pattern of therapy depending on the clinical condition of the patient. The purpose of this study was to obtain data on the patterns and treatment of oral diseases due to HSV-1 infection. Methods: Data collected was secondary data from the logbook and medical records of patients at the Dental Polyclinic of Dr Hasan Sadikin Hospital Bandung in 2013-2017. The study sample was determined using a purposive sampling method, namely medical records of patients with a diagnosis of oral disease due to HSV-1 infection. Results: The pattern of oral disease in outpatient installations, namely Recurrent Intraoral Herpes (RIH) 84.91%, Associated Erythema Multiforme (HAEM) Herpes 9.43%, Herpes labialis 3.77%, and Primary Herpetic Gingivo Stomatitis (PHGS) 1.89%. The pattern of oral disease in inpatient installations, namely Recurrent Intraoral Herpes (RIH) as much as 85.71% and Herpes labialis 14.29%. Given therapy was very varied, namely a combination of acyclovir, antiseptic, multivitamin, anti-inflammatory steroid, NSAIDs, lip moisturisers, antibiotics, antihistamines, and antifungal. Conclusion: Oral disease due to HSV-1 infection that is most often found both in outpatient and inpatient installations is recurrent intraoral herpes. The most commonly used therapy in outpatient installations is a combination of steroid anti-inflammatory drugs and multivitamins, while in inpatient installations are multivitamins and a combination of acyclovir, antiseptic, and multivitamins.Keywords: Oral disease patterns, therapy patterns, Herpes Simplex Virus-1 infection

Uji daya antibakteri ekstrak etanol kulit buah manggis (Garcinia mangostana L) terhadap Streptococcus sanguinis ATCC 10556 Dwita Kemala; Ina Hendiani; Mieke Hemiawati Satari
Padjadjaran Journal of Dental Researchers and Students Vol 2, No 2 (2018): Oktober 2018
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjdrs.v3i1.21447

Pendahuluan: Pemeliharaan kesehatan gigi dengan cara menyikat gigi dan penggunaan obat kumur dapat mencegah pembentukan biofilm oral yang dipelopori oleh Streptococcus sanguinis. Kulit buah manggis (Garcinia mangostana L.) adalah kulit buah yang telah diketahui memiliki aktifitas antibakteri. tujuan penelitian mengetahui daya anti bakteri ekstrak etanol kulit buah manggis (Garcinia Mangostana L.) terhadap pertumbuhan Streptococcus sanguinis ATCC 10556. Metode: Jenis penelitian ini adalah deskriptif, untuk mengetahui uji daya antibakteri ekstrak etanol kulit buah manggis (Garcinia mangostana L.) pada Streptococcus sanguinis ATCC 10556. Populasi bahan uji penelitian ini adalah kulit buah manggis (Garcinia mangostana L.) yang ada di Kota Bogor. Sampel yang digunakan adalah kulit buah manggis (Garcinia mangostana L.) yang didapat dari kebun budidaya IPB Bogor. Metode uji hambat yang dinilai dengan menggunakan metode disk diffusion. Hasil: Diameter zona hambat paling besar terdapat pada konsentrasi 200.000 ppm dengan diameter rata-rata 12,55 mm, di ikuti dengan konsentrasi 100.000 ppm dengan diameter rata-rata 11,2 mm, kemudian konsentrasi 50.000 ppm dengan diameter rata-rata 10,3 mm, selanjutnya konsentrasi 20.000 ppm dengan konsentrasi rata-rata 10,05 mm, kosentrasi 10.000 ppm dengan diameter rata-rata 8,2 mm, konsentrasi 2.000 ppm dengan diameter rata-rata 7,1 mm dan diameter zona hambat paling kecil terdapat pada konsentrasi 1.000 ppm dengan diameter 6,9 mm. Simpulan: Ekstrak etanol kulit buah manggis (Garcinia mangostana L.) memiliki daya antibakteri terhadap Streptococcus sanguinis ATCC 10556.Kata kunci: Daya antibakteri, ekstrak etanol kulit buah manggis, Garcinia mangostana L., Streptococcus sanguinis

Prevalensi dan pola penyakit infeksi virus rongga mulut di RSUP Dr. Hasan Sadikin Bandung tahun 2013-2017Prevalence and patterns of oral viral infection in Dr Hasan Sadikin Hospital Bandung in 2013-2017 Husnul Mahfaza; Irna Sufiawati; Mieke Hemiawati Satari
Padjadjaran Journal of Dental Researchers and Students Vol 3, No 1 (2019): Februari 2019
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjdrs.v3i1.22180

Pendahuluan: Terdapat sejumlah virus yang dapat menyebabkan infeksi daerah perioral, mulut, dan orofaring. Virus dapat menginisiasi infeksi, menyebar ke seluruh tubuh, dan bereplikasi berdasarkan sifat dan karakteristik virulensinya. Penyakit akibat virus dapat menyebabkan kerusakan sel secara langsung maupun dapat berupa reaksi sekunder. Virus yang menyerang rongga mulut dapat bermanifestasi diantaranya seperti vesikula dan ulser. Penelitian ini bertujuan untuk mengetahui prevalensi dan pola penyakit infeksi virus rongga mulut di RSUP Dr. Hasan Sadikin Bandung tahun 2013-2017. Metode: Penelitian deskriptif dengan menggambarkan prevalensi dan pola penyakit infeksi rongga mulut. Data yang dikumpulkan merupakan data sekunder berbentuk rekam medis pasien. Pengambilan sampel dilakukan dengan cara purposive sampling, dengan kriteria inklusi yaitu pasien dengan diagnosis infeksi virus rongga mulut di RSUP Dr. Hasan Sadikin Bandung tahun 2013-2017. Hasil: Penelitian ini menunjukkan total pasien rawat inap berjumlah 742 orang, didapatkan 21 pasien dengan infeksi virus rongga mulut, yang paling banyak ditemukan yaitu HSV-1 (66,67%), diikuti oleh infeksi HSV-2 (4,76%), CMV (9,52%), dan VZV sebanyak (19,05%). Sedangkan pada pasien rawat jalan yang berjumlah 531 orang didapatkan 58 pasien dengan infeksi virus rongga mulut, infeksi HSV-1 juga paling banyak ditemukan (91,38%), diikuti oleh infeksi CMV (1,72%), dan VZV (6,90%). Simpulan: Prevalensi infeksi virus rongga mulut sebanyak 2,83% pada pasien rawat inap dan sebanyak 10,92% pada pasien rawat jalan, dengan jumlah tertinggi yaitu infeksi HSV-1.Kata kunci: Infeksi virus, orofasial, famili herpes virus ABSTRACTIntroduction: Several viruses can cause infection of the perioral region, mouth, and oropharynx. Viruses can initiate infections, spread throughout the body, and replicate based on their virulence characteristics. Viral diseases can cause cell damage directly or can trigger a secondary reaction. Viruses that attack the oral cavity can manifest, such as vesicles and ulcers. This study was aimed to determine the prevalence and patterns of oral viral infection in Dr Hasan Sadikin Hospital Bandung in 2013-2017. Methods: Descriptive study was performed by describing the prevalence and pattern of oral infections. Data collected was secondary data in the form of patient medical records. Sampling was carried out by purposive sampling, with inclusion criteria, namely patients with a diagnosis of oral cavity virus infection in Dr Hasan Sadikin Hospital Bandung in 2013-2017. Results: This study showed a total of 742 inpatients, 21 patients with oral cavity infections, the most common were HSV-1 (66.67%), followed by HSV-2 infection (4.76%), CMV (9.52%), and VZV infections (19.05%). Whereas in total 531 outpatients, 58 patients were found with oral cavity infections, HSV-1 infections were also found the most (91.38%), followed by CMV (1.72%), and VZV infections (6.90%). Conclusion: The prevalence of oral cavity infection is 2.83% in inpatients and as many as 10.92% in outpatients, with the highest number being HSV-1 infection.Keywords: Viral infection, orofacial, herpes virus family

THE CORRELATION BETWEEN TERPENOID OF SARANG SEMUT (Myrmecodia pendans) WITH mRNA FRUCTOSYLTRANFERASE EXPRESSION OF Streptococcus mutans BIOFILM ATCC 25175 Mieke Hemiawati Satari; Eka Chemiawan; Meirina Gartika; Darul Rabil
ODONTO : Dental Journal Vol 8, No 1 (2021): July 2021
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.8.1.86-93

Background: Terpenoid isolate sarang semut (Myrmecodia pendans) is a natural material that has antibacterial power through the destruction of the cell membrane of Streptoccus mutans (S. mutans). Fructosiltransferase (ftf) is one of the extrapolisaccharides produced by S. mutans. The formation of Ftf is governed by the ftf gene. The aim of research was to know the correlation between terpenoid of Sarang semut administration with ftf mRNA expression of S. mutans biofilm ATCC 25175.Method: The study used mixed methods, namely descriptive explorative method to look at the isolation of ftf gene and ftf mRNA expression. This study began with making 1 day biofilm , then given terpenoid isolates of sarang semut and chlorhexidine. Next, isolation of ftf genes and ftf mRNA expression was carried out, then the differences in S. mutans ATCC 25175 mRNA ftf expression in sarang semut and chlorhexidine were given for 1 and 30 minutes using Real-Time PCR. Continued by looking at the relationship of the duration of administration of sarang semut terpenoids to the expression of S. mutans ATCC 25175 mRNA ftf biofilm. Statistical analysis used the t test with p-value <0.05 and Pearson.Results: The results showed that ftf gene with 130 bp length and ftf mRNA expression could be isolated from S. mutans biofilm ATCC 25175 which were given terpenoid isolates from sarang semut. The expression of sarang semut mRNA ftf and chlorhexidine did not have a significant difference for 1 minute (p = 0.0761), whereas in 30 minutes the ant nest had a higher mRNA expression (p = 0.0186). There is a relationship between the duration of administration of terpenoid isolates of sarang semut with S. mutans ATCC 25175 mRNA ftf expression (r2 = 0.614), with a medium correlation coefficient.Conclusion: The conclusion of the study were that sarang semut terpenoid had the same effect as chlorhexidine for 1 minute on S. mutans biofilms, whereas at 30 minutes it was different. There is a relationship between terpenoids of sarang semut isolates and S. mutans biofilm ATCC 25175 mRNA expression.

Perbedaan potensi antibakteri ekstrak metanol umbi sarang semut (Myrmecodia pendens Merr. & Perry) dan NaOCl terhadap Streptococcus mutans (ATCC 25175)The antibacterial potential differences between the methanolic extract of ant-plant (Myrmecodia pendens Merr. & Perry) tubers and NaOCl towards Streptococcus mutans (ATCC 25175) Eria Ariningtyas Apriyanti; Mieke Hemiawati Satari; Bremmy Laksono
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 28, No 2 (2016): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Pendahuluan: Streptococcus mutans merupakan organisme kariogenik utama. Antibakteri sintetik yang biasa digunakan adalah NaOCl. Sarang semut mengandung senyawa kimia yang dapat menghambat pertumbuhan Streptococcus mutans. Tujuan penelitian ini adalah mengetahui daya hambat ekstrak metanol sarang semut (Myrmecodia pendans Merr. & Perry) dan NaOCl terhadap Streptococcus mutans serta perbedaan potensi antibakteri keduanya. Metode: Jenis penelitian adalah eksperimental laboratoris. Uji daya antibakteri dilakukan dengan menggunakan metode difusi agar. Media agar darah ditetesi 0,1 ml suspensi bakteri lalu dimasukkan ekstrak metanol sarang semut dengan konsentrasi 30%, 15%, 7,5%, 3,75%, 1,875% dan NaOCl dengan konsentrasi 5%, 2,5%, 1,25% diinkubasi pada suhu 37° C selama 24 jam dalam suasana fakultatif anaerob dan dilakukan replikasi tiga kali. Uji statistik menggunakan metode ANAVA dan Independent t-test. Hasil: Ekstrak metanol sarang semut konsentrasi 30% menghasilkan diameter zona hambat rata-rata 5,87 mm, sedangkan NaOCl 1,25% adalah 9,33 mm. Simpulan: Ekstrak metanol sarang semut dan NaOCl memiliki potensi daya hambat terhadap Streptococcus mutans, namun potensi antibakteri NaOCl lebih besar dibandingkan dengan ekstrak metanol sarang semut.Kata kunci: Antibakteri, ekstrak metanol, sarang semut, NaOCl, Streptococcus mutans. ABSTRACTIntroduction: Streptococcus mutans is the main cariogenic organism. The synthetic antibacterial commonly used is NaOCl. Ant nests contain chemical compounds that can inhibit the growth of Streptococcus mutans. The purpose of this study was to determine the inhibitory power of methanol extracts of ant nests (Myrmecodia pendans Merr. & Perry) and NaOCl against Streptococcus mutans and the differences in antibacterial potential both. Methods: This type of research is experimental laboratory. Antibacterial power test was carried out using agar diffusion method. Blood agar media was dripped with 0.1 ml of bacterial suspension and then inserted methanol extract of ant nests with a concentration of 30%, 15%, 7.5%, 3.75%, 1.875% and NaOCl with a concentration of 5%, 2.5%, 1, 25% was incubated at 37°C for 24 hours in a facultative anaerobic atmosphere and replicated three times. Statistical test using ANAVA method and Independent t-test. Result: Methanol extract of ant nest 30% resulted in an average inhibition zone diameter of 5.87 mm, while 1.25% NaOCl was 9.33 mm. Conclusion: Methanol extract of ant nests and NaOCl has potential inhibitory power against Streptococcus mutans, but the antibacterial potency of NaOCl is greater than that of methanol extracts of ant-plants.Keywords: Antibacterial, methanolic extract, ant-plants, NaOCl, Streptococcus mutans.

Perbedaan jumlah koloni Streptococcus mutans pada perokok kretek dan bukan perokokDifference between the number of Streptococcus mutans colonies in kretek smokers and non-smokers Intan Melani; Mieke Hemiawati Satari; Yuti Malinda
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 30, No 2 (2018): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Pendahuluan: Rokok kretek merupakan jenis rokok yang paling populer di Indonesia. Merokok dapat menyebabkan berbagai gangguan dalam rongga mulut, salah satunya yaitu karies. Streptococcus mutans merupakan salah satu bakteri yang berperan penting dalam proses terjadinya karies. Penelitian ini bertujuan untuk mengetahui perbedaan jumlah koloni Streptococcus mutans pada perokok kretek dan bukan perokok. Metode: Penelitian deskriptif ini dilakukan dengan teknik pengambilan sampel consecutive sampling. Jumlah sampel yang diperoleh 20 orang yang terdiri dari 10 perokok kretek dan 10 bukan perokok. Bahan pemeriksaan berupa saliva diambil dari masing-masing sampel. Sampel saliva diencerkan, dieramkan dan diinokulasi pada media TYCSB. Sampel saliva kemudian diinkubasi pada 370C, secara fakultatif anaerob selama 48 jam. Koloni yang tumbuh dihitung menggunakan colony counter. Data dianalisis menggunakan t-test independen (α=0,05). Hasil: Rata-rata jumlah koloni Streptococcus mutans dari saliva perokok kretek sebesar 47,65 x 102 CFUs/ml sedangkan pada bukan perokok sebesar 11,1 x 102 CFUs/ml. Data statistik uji t independen mendapatkan nilai p<0,05 yang berarti terdapat perbedaan yang signifikan antara jumlah koloni Streptococcus mutans pada perokok kretek dan bukan perokok. Simpulan: Jumlah koloni Streptococcus mutans pada perokok kretek lebih tinggi dibandingkan bukan perokok.Kata kunci: Rokok kretek, bukan perokok, perokok kretek, Streptococcus mutans. ABSTRACTIntroduction: Kretek cigarettes are the most popular type of cigarette in Indonesia. Smoking can cause various oral health problems, one of them is caries. Streptococcus mutans is one of the essential bacteria in the process of caries. This study was aimed to determine the difference between the number of Streptococcus mutans colonies in kretek smokers and non-smokers. Methods: A descriptive study was carried out with the consecutive sampling technique. Samples obtained were as much as 20 people consisted of 10 kretek smokers and 10 non-smokers. Examination material was the saliva taken from each sample. The saliva were diluted, incubated, and inoculated on TYCSB media. Salivary samples were incubated at 37°C in facultative anaerob environment for 48 hours. The growing colonies were calculated using a colony counter. Data obtained were analysed using independent t-test (α = 0.05). Result: The average number of Streptococcus mutans colonies from kretek smoker saliva was 47.65 x 102 CFUs / ml, while in the non-smokers was 11.1 x 102 CFUs / ml. Independent t-test results were obtained the p-value < 0.05, which means that there was a significant difference between the number of Streptococcus mutans colonies in kretek smokers and non-smokers. Conclusion: The number of Streptococcus mutans colonies in kretek smokers saliva was higher than the non-smokers.Keywords: Kretek cigarettes, non-smokers, kretek smokers, Streptococcus mutans.

Daya hambat ekstrak metanol nanas, belimbing wuluh, dan kemangi terhadap Streptococcus mutans ATCC 25175Inhibitory potential of methanolic extract of pineapple, wuluh starfruit, and basil towards Streptococcus mutans ATCC 25175 Yona Ayumi Budiani; Mieke Hemiawati Satari; Tadeus Arufan Jasrin
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 29, No 2 (2017): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Pendahuluan: Karies merupakan suatu penyakit infeksi dimana terjadi proses demineralisasi progresif pada jaringan keras gigi. Proses demineralisasi gigi dipengaruhi oleh pH asam pada mulut yang disebabkan oleh Streptococcus mutans. Tujuan peneltian adalah untuk menguji daya hambat dari ekstrak metanol nanas, belimbing wuluh, dan kemangi terhadap Streptococcus mutans sebagai bakteri yang berperan dalam proses pembentukan plak serta karies gigi. Metode: Penelitian dilakukan secara eksperimental dengan cara meneteskan ekstrak metanol nanas, belimbing wuluh, dan kemangi, serta ekstrak kombinasi ketiganya yang telah diencerkan dengan pelarut Dimetil Sulfoksida (DMSO) ke dalam lubang sumuran pada lempeng agar Mueller Hinton yang telah ditanami Streptococcus mutans. Bakteri uji berupa Streptococcus mutans ATCC 25175 yang diperiksa secara mikroskopis, ditanam pada lempeng agar Muller Hinton dan inkubasi dalam suasana fakultatif anaerob pada suhu 37OC selama 24 jam. Konsentrasi ekstrak yang diuji adalah 20.000 ppm, 10.000 ppm. 5.000 ppm, dan 1.000 ppm. Hasil: Hasil penelitian menunjukan bahwa ekstrak metanol nanas, belimbing wuluh, dan kemangi memiliki daya hambat terhadap Streptococcus mutans ATCC 25175. Simpulan: Daya hambat yang terjadi pada bakteri disebabkan oleh kandungan zat aktif yang dimiliki nanas, belimbing wuluh, dan kemangi seperti enzim bromelain, flavonoid, tanin, alkaloid, triterpenoid, dan saponin.Kata kunci: Streptococcus mutans, nanas, Ananas comosus, belimbing wuluh, Averrhoa bilimbi, kemangi, Ocimum tenuiflorum, uji daya hambat. ABSTRACTIntroduction: Caries is an infectious disease in which there is a progressive demineralization process in hard teeth. The demineralization process of teeth is affected by acid pH in the mouth caused by Streptococcus mutans. The aim of the study was to examine the inhibitory potential of methanol extract of pineapple, starfruit and basil to Streptococcus mutans as bacteria that play a role in the process of plaque formation and dental caries. Methods: The study was carried out experimentally by dripping the methanol extract of pineapple, starfruit and basil, and the combination of the three extracts which had been diluted with Dimethyl Sulfoxide (DMSO) solvent into the well hole on the agar plate of Mueller Hinton which had been planted with Streptococcus mutans. Test bacteria in the form of ATCC 25175 Streptococcus mutans were examined microscopically, planted on Muller Hinton agar plate and incubated in anaerobic facultative atmosphere at 37OC for 24 hours. The concentration of extract tested was 20,000 ppm, 10,000 ppm. 5,000 ppm, and 1,000 ppm. Result: The results showed that the methanol extract of pineapple, starfruit and basil had inhibitory potential against Streptococcus mutans ATCC 25175. Conclusion: The inhibitory potential that occurs in bacteria is caused by the active substance possessed by pineapple, starfruit, and basil such as bromelain enzyme, flavonoids, tannins, alkaloids, triterpenoids, and saponins.Keywords: Streptococcus mutans, pineapple, Ananas comosus, starfruit, Averrhoa bilimbi, basil, Ocimum tenuiflorum, inhibitory test.

Role of Streptococcus Anginosus on the formation of dental caries Yetty Herdiyati Nonong; Mieke Hemiawati Satari; Inne Suherna Sasmita
Padjadjaran Journal of Dentistry Vol 23, No 3 (2011): November 2011
Publisher : Faculty of Dentistry Universitas Padjadjaran

Generally, the etiology of dental caries is the cariogenic properties of bacteria, these are always associated with Streptococcus mutans. Glucosyltransferase fragment (Gtf) are also in other strains of Streptococcus such as Streptococcus anginosus, Streptococcus milleri which includes beta hemolysis. Genotypically B Streptococcus anginosus has genetic characteristics that are similar to Streptococcus mutans. The research objective was to determine the existence of Gtf B/C gene as a cause of caries in Streptococcus anginosus. The study was conducted in experimental laboratories with PCR technique by taking a sample of 20 children who had caries. The results showed there was the amplification of Streptococcus anginosus with a level of homology 96%, 97%, and 99%. The results of the Gtf genes amplification fragment B/C provided 600 pb ribbon. The conclusion was Streptococcus anginosus classified as cariogenic bacteria because they had Gtf B/C genes.

The effect of ethanol extract of mangostene (Garcinia Mangostana Linn.) peel on tongue cancer cells Supri's Clone-1 apoptosis, in vitro Maria Aurora Titiana; Roosje Rosita Oewen; Mieke Hemiawati Satari
Padjadjaran Journal of Dentistry Vol 23, No 3 (2011): November 2011
Publisher : Faculty of Dentistry Universitas Padjadjaran

Apoptosis or programmed cell death serves to regulate physiological and pathological conditions. Garcinia mangostana Linn. is one of the medical herbs that is widely used to suppress human tongue cancer growth by inducing apoptosis. The research aimed to analyze the effect of ethanol extract of Garcinia mangostana Linn. on tongue cancer cells apoptosis Supri's Clone-1 (SP-C1). The type of the research was an experimental laboratory. Tongue cancer cells SP-C1 were treated by various ethanol extract concentrations (0, 300, 400, 500, 600, 700 pg/ml) of Garcinia mangostana Linn. to detect the apoptosis, which was done by acridine orange and ethidium bromide coloring tests. The number of tongue cancer cells SP-C1 2x104 cells/dish. Observation on cells apoptosis was observed by a fluorescent microscope with 40 x magnification. The data were analyzed using one-way ANOVA and was followed by Post Hoc test (Tukey-test) with 95% significance level. The result showed that tongue cancer cells SP-C1 apoptosis treated by various ethanol extracts of Garcinia mangostana Linn. increase significantly. The highest effect of apoptosis was detected at the 700 pg/ml concentration that has an effect on the percentage of tongue cancer cells apoptosis by 65%.

Anti-tumor agent celecoxib activity towards SP-C1 tongue cancer cells invasion (in vitro) Harun Achmad; Mieke Hemiawati Satari; Roosje Rosita Oewen; S. Supriatno
Padjadjaran Journal of Dentistry Vol 23, No 1 (2011): March 2011
Publisher : Faculty of Dentistry Universitas Padjadjaran

Invasion is a characteristic of the occurrence of cancer and indicates the cancer cells' capability to destroy and degrade the border between the epithet and basal membrane to further spread into the surrounding extra-cellular matrix. The purpose of this research was to find the existence of impediment at the SP-C1 tongue cancer cell using celecoxib chemopreventive medication. The SP-C1 tongue cancer cells were treated in vitro using celecoxib medication as a research subject at the following concentrations 5, 10, 25, 50, 75, 100, 125%; and 0 as control group (only DMEM growth medium treatment). Pure experimental testing was carried out for 24 and 48 hours, with observation and calculation of an average number of SP-C1 tongue cancer cells. The data collected were analyzed using the ANOVA test with Newman Keuls paired range test or t-test. Research results indicated that the average number of SP-C1 tongue cancer cells invasion after administration of celecoxib medication based on administration concentration and time statistically yielded significant results. The ANOVA test results were statistically significant, that is, average occurrence of the number of SP-C1 tongue cancer cells due to the use of celecoxib at certain concentrations compared to that without celecoxib was different. At celecoxib of zero (control) concentration was 24.4 with celecoxib concentration starting at 5 up to 125% experienced a decline from its average 11 to become 2.3. The conclusion of the research was that the greater the celecoxib concentration administered, the greater the effect on the impediment of SP-C1 tongue cancer cell invasion.

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