Haris Budi Widodo
Universitas Jenderal Soedirman

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EFFECT OF TORCH GINGER EXTRACT (Etlingera elatior) ON THE ERADICATION OF Aggregatibacter actinomycetemcomitans BIOFILM ANS CAUSES AGGRESSIVE PERIODONTITIS Meylida Ichsyani; Haris Budi Widodo; Rifda Naufalin; Aisha Tiara Dewi; Adninda Rimawati; Devi Anisya Putri; Novita Dwi Lokasari
Mandala Of Health Vol 13 No 2 (2021): Mandala Of Health
Publisher : Fakultas Kedokteran Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mandala.2021.13.2.8745

Abstract

Aggregatibacter actinomycetemcomitans is an oral microflora that forms biofilms and is the main cause of aggressive periodontitis. Long-term mouthwash therapy can cause mucositis and even oral cancer. The ethanol extract of torch ginger (Etlingera elatior) is reported to have analgesic, anti-inflammatory, and antibacterial activities, so it can be developed as an alternative to prevention or adjuvant therapy for aggressive periodontitis. The aim of this study was to determine the effect of the ethanol extract of the torch ginger plant on the biofilm degradation of A. actinomycetemcomitans. The study used ethanol extracts of torch ginger flowers, leaves, and stems at concentrations of 1.56 mg/mL, 3.125 mg/mL, 6.25 mg/mL, 12.5 mg/mL, 25 mg/mL, and 50 mg/mL. CHX 0.2% mouthwash was used as a positive control, while 1% DMSO was used as a negative control. The biofilm degradation test was carried out using a microtiter plate assay with 1% crystal violet staining at a wavelength of 450 nm. The results showed that there was significant activity against biofilm degradation of A. actinomycetemcomitans in the administration of an ethanol extract of torch ginger flowers, leaves, and stems compared to the negative control (p<0.05). The optimum concentrations of biofilm degradation by extracts of flowers, leaves, and stems were 25 mg/mL (85.54%), 25 mg/mL (84.43%), and 50 mg/mL (72.10%), respectively. MBEC50 values of flowers are 4.82 mg/mL, MBEC50 leaves are 5.96 mg/mL, and MBEC50 stems are 10.15 mg/mL. The conclusion of this study is that there is an effect of giving an ethanol extract of torch ginger flowers, leaves, and stems on the biofilm degradation of A. actinomycetemcomitans bacteria.
The Correlation of Oral Health Knowledge and Affective with Caries Rate in Rural Communities Christiana Cahyani Prihastuti; Fitri Diah Oktadewi; Mahindra Awwaludin Romdlon; Amilia Ramadhani; Haris Budi Widodo; Diah Krisnansari; Fitranto Arjadi
Insisiva Dental Journal: Majalah Kedokteran Gigi Insisiva Vol 12, No 1 (2023): May
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/di.v12i1.15681

Abstract

The results of Riskesdas (Baseline Health Research) by the Indonesian Ministry of Health in 2018 revealed that the caries rate in rural areas was higher than in urban areas. However, the number of villagers receiving dental and oral health care was lower than in urban communities. Karangtengah Village was located in the Baturraden District, Banyumas Regency, which is socio-demographically rural. This study aims to determine the correlation between knowledge and affective of oral health on the caries rate in Karangtengah Village. The study was observational analytical research. Subjects were selected using a purposive sampling technique. The total subjects were 388 residents. The knowledge and affective data were collected using a questionnaire, and the caries rate was scored using DMF-T. Data were analyzed using the Pearson correlation test (SPSS 22). The results revealed that the oral health knowledge and affective of the majority of Karangtengah Village residents were in the moderate category (48.7% and 66.8%, respectively), while the average DMF-T score was 13.12 (very high). The statistical analysis demonstrated no significant correlation between knowledge and DMF-T score and between the affective aspect of oral health and DMF-T score (p0.5).  The moderate category of oral health knowledge and affective aspect in rural areas may not have been applied as oral health behaviors contribute to high dental caries rates.