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All Journal Medali Jurnal : Media Dental Intelektual Majalah Kedokteran Gigi Indonesia Jurnal Kedokteran Gigi Universitas Padjadjaran Journal of Indonesian Dental Association Scripta Biologica Mandala of Health : A Scientific Journal Biomedika Journal of Dental and Biosciences
Meylida Ichsyani
Universitas Jenderal Soedirman
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Medicine & Pharmacology Public Health

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PENGARUH DIET PROTEIN DAN SUPLEMENTASI VITAMIN D3 PADA MASA MENYUSUI TERHADAP ERUPSI GIGI INSISIVUS SENTRALIS MANDIBULA ANAK TIKUS MALNUTRISI Riski Amalia Hidayah; Rakmawati Rakmawati; Aris Aji Kurniawan; Anindita Laksitasari; Meylida Ichsyani
Scripta Biologica Vol 10, No 1 (2023)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2023.10.1.1296

Abstract

ABSTRAKMalnutrisi akibat kekurangan protein saat kehamilan berisiko melahirkan bayi berat badan lahir rendah (BBLR) dan berdampak pada pertumbuhan tulang dan gigi anak. Kekurangan protein saat kehamilan dapat menyebabkan terjadinya gangguan mineralisasi gigi, ganggguan maturasi gigi, hambatan erupsi gigi,  kegoyahan gigi bahkan kehilangan gigi akibat penurunan kualitas tulang alveolar. Protein sangat dibutuhkan dalam proses metabolisme kalsium dalam tulang dan gigi. Vitamin D3 berperan dalam homeostasis kalsium dan fosfor yang dibutuhkan untuk mineralisasi jaringan keras termasuk tulang dan gigi. Tujuan dari penelitian ini adalah untuk mengetahui umur pertama erupsi dan panjang pertumbuhan gigi insisivus mandibula sentralis kanan dan kiri saat masa erupsi pada anak malnutrisi yang menyusu pada induk dengan perlakuan diet protein dan suplementasi vitamin D3. Penelitian ini termasuk jenis penelitian eksperimental laboratoris dengan rancangan post test only control group design.  Sampel penelitian menggunakan 30 ekor tikus Rattus novergicus galur wistar yang dibagi menjadi 5 kelompok yaitu 2 kelompok kontrol meliputi kontrol positif (K+) berupa anak tikus dengan berat badan (BB) normal yang menyusu pada induk yang diberikan perlakuan diet protein standar dan suplementasi vitamin D dosis 0,036 IU/ hari peroral serta kontrol negatif (K-) berupa anak tikus malnutrisi yang menyusu pada induk yang diberikan perlakuan diet protein rendah selama masa menyusui. Kelompok perlakuan meliputi 3 kelompok anak tikus malnutrisi yang menyusu pada induk dengan diet protein standar dengan suplementasi vitamin D dosis 0,036 IU/ hari peroral (P1), diet protein standar dengan suplementasi vitamin D dosis 0,036 IU/ hari peroral (P2) serta diet protein standar saja selama masa menyusui. Hari pertama erupsi diukur secara visual dan panjang pertumbuhan gigi diukur pada umur 14 hari dan 22 hari dengan menggunakan sliding caliper. Hasil penelitian menunjukkan bahwa erupsi paling cepat terjadi pada kelompok K+ kemudian P1. Kelompok P2 dan P3 mengalami kecepatan erupsi yang sama yaitu pada rerata umur 10,2 hari, sedangkan kelompok K- mengalami erupsi gigi paling lambat yaitu mencapai rerata umur 11,5 hari. Pemberian diet protein standar dan vitamin D3 dosis 0,036 IU/hari peroral menunjukkan perbedaan yang bermakna terhadap panjang pertumbuhan gigi. Pemberian diet protein standar menunjukkan perbedaan bermakna terhadap panjang pertumbuhan gigi. Pemberian diet protein rendah dan vitamin D3 dosis 0,036 IU/hari peroral menunjukkan perbedaan bermakna terhadap panjang pertumbuhan gigi insisivus sentralis mandibula kiri namun tidak terdapat perbedaan yang bermakna pada regio kanan. Kombinasi diet protein standar dengan vitamin D3 dosis 0,036 IU/ hari per oral yang saat masa menyusui mempercepat waktu erupi gigi dan meningkatkan panjang pertumbuhan gigi insisivus sentralis mandibula kanan dan kiri anak tikus dari umur 14 hingga 22 hari. 
Isolasi dan karakterisasi bakteri kariogenik pada pasien di Rumah Sakit Gigi dan Mulut Universitas Jenderal Soedirman Rinawati Satrio; Supriyati Supriyati; Fadli Ashar; Salwa Az-Zahra; Dwi Nur Indah Sari; Meylida Ichsyani
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 35, No 1 (2023): April 2023
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkg.v35i1.41439

Abstract

ABSTRAKPendahuluan: Karies gigi adalah hilangnya struktur kimia penyusun gigi yang disebabkan oleh interaksi kompleks antara mikroorganisme rongga mulut pada plak gigi, diet dan faktor host. Pencegahan karies dapat dilakukan dengan manipulasi bakteri yang diarahkan pada bakteri kariogenik dominan yang diisolasi dari karies. Penelitian ini bertujuan untuk mengisolasi dan mengkarakterisasi bakteri kariogenik pada pasien yang terdiagnosis pulpitis di Rumah Sakit Gigi dan Mulut Universitas Jenderal Soedirman pada bulan Desember 2020 – Februari 2021. Metode: Penelitian observasional deskriptif ini dilakukan dengan consecutive sampling. Sampel penelitian diambil dari karies gigi dengan kedalaman dentin pada 5 pasien di RSGM Unsoed yang terdiagnosis pulpitis. Identifikasi dilakukan dengan menumbuhkan bakteri pada media agar darah, Mannitol Salt Agar (MSA), dan Man Rogosa Sharpe Agar (MRSA) kemudian dilakukan pengamatan morfologi, perhitungan koloni bakteri, pewarnaan Gram dan uji-uji biokimia. Hasil : Jenis bakteri yang dapat diidentifikasi dari karies adalah Staphylococcus aureus (80%), Staphylococcus intermedius (40%), Coagulase Negative Staphylococcus (60%), Lactobacillus sp (80%) dan Lactobacillales Gram positif (20%). Simpulan: Didapatkan isolat yang diduga bakteri Staphylococcus aureus, Staphylococcus intermedius, Coagulase Negative Staphylococcus (CoNS), Lactobacillus sp dan Lactobacillales Gram positif yang diisolasi dari karies gigi.Kata kunci: karies gigi, bakteri kariogenik, staphylococcus aureus, lactobacillus spIsolation and characterization of cariogenic bacteria in patient at Universitas Jenderal Soedirman Dental HospitalABSTRACTIntroduction: Dental caries is the loss of the chemical structure of the teeth that is caused by complex interactions between oral microorganisms on dental plaque, diet and host factors. Caries prevention can be done by manipulation of bacteria directed at the dominant cariogenic bacteria isolated from caries. This study aims to isolate and characterize cariogenic bacteria in patients at the Universitas Jendral Soedirman Dental Hospital from Desember 2020 until Februari 2021. Methods: This descriptive observational study was conducted with consecutive sampling. Samples were taken from dental caries at dentine depth in 5 patients at Universitas Jenderal Soedirman Dental Hospital who were diagnosed with pulpitis. Identification was done by growing bacteria on blood agar media, Mannitol Salt Agar (MSA) and Man Rogosa Sharpe Agar (MRSA) then morphological observations, bacterial colony counting, Gram staining and biochemical tests were done. Results: The types of bacteria that can be identified from caries are Staphylococcus aureus (80%), Staphylococcus intermedius (40%), Coagulase Negative Staphylococcus (60%), Lactobacillus sp. (80%) and Gram positive Lactobacilllales (20%). Conclusion: We found isolates suspected to be Staphylococcus aureus, Staphylococcus intermedius, Coagulase Negative Staphylococcus (CoNS), Lactobacillus sp and Gram positive Lactobacilllales isolated from dental caries.Keywords: dental caries, cariogenic bacteria, staphylococcus aureus, lactobacillus sp
Aktivitas antibiofilm ekstrak etanol kulit bawang merah (Allium cepa L.) dalam menghambat pembentukan biofilm Staphylococcus aureus ATCC 25923 Alya Ghina Rosyada; Christiana Cahyani Prihastuti; Dwi Nur Indah Sari; Setiawati Setiawati; Meylida Ichsyani; Anindita Laksitasari; Restian Febi Andini; Aris Aji Kurniawan
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 35, No 1 (2023): April 2023
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkg.v35i1.42451

Abstract

ABSTRAKPendahuluan: Permasalahan dental maupun medis banyak disebabkan oleh biofilm bakteri di dalam rongga mulut. Salah satu bakteri penyusun biofilm yaitu Staphylococcus aureus. Bakteri ini merupakan jenis bakteri Gram positif yang bersifat patogen oportunistik dalam rongga mulut serta memiliki mekanisme pembentukan biofilm yang kompleks. Kulit bawang merah mengandung senyawa fitokimia seperti flavonoid, saponin, fenol, tanin, alkaloid, dan steroid yang dilaporkan bersifat antibiofilm sehingga berpotensi sebagai alternatif obat kumur. Penelitian ini bertujuan untuk menganalisis aktivitas antibiofilm ekstrak etanol kulit bawang merah (EKBM) konsentrasi 6,25%; 12,5%; 25%; 50%; dan 100% dalam menghambat pembentukan biofilm S. aureus. Metode: Penelitian ini meruoakan penelitian eksperimental laboratoris in vitro dengan rancangan penelitian posttest-only control group design.Ekstrak dibuat menggunakan metode maserasi etanol 96% dengan perendaman selama 15 hari. Kontrol positif penelitian ini adalah CHG 0,2% dan kontrol negatif berupa DMSO 1%. Penghambatan pembentukan biofilm diukur menggunakan Microtiter Plate Assay (MPA) dengan panjang gelombang 595 nm. Data hasil pembacaan MPA dianalisis secara statistik dengan uji Kruskal-Wallis dan uji post hoc Mann-Whitney. Hasil: Persentase penghambatan biofilm tertinggi ada pada EKBM 25% (92,86+1,45%) namun hasil ini masih lebih rendah daripada kelompok kontrol positif (94,74+0,56%) secara bermakna (p<0,05). Tidak terdapat perbedaan pengaruh yang bermakna (p<0,05) antara persentase penghambatan pembentukan biofilm oleh EKBM 12,5% (92,74+0,98%) dengan EKBM 25% secara statistik. Simpulan: Penghambatan pembentukan biofilm oleh EKBM terhadap biofilm S. aureus yang paling efektif terdapat pada konsentrasi 12,5%.Kata kunci: Allium cepa L, biofilm, kulit bawang merah, Staphylococcus aureus.ABSTRACTIntroduction: Many dental and medical problems are caused by bacterial biofilms in the oral cavity. One of the bacteria that form the biofilm is Staphylococcus aureus. This bacterium is a type of Gram-positive bacteria which is an opportunistic pathogen in the oral cavity and has a complex biofilm formation mechanism. Shallot peel contains phytochemical compounds such as flavonoids, saponins, phenols, tannins, alkaloids, and steroids which are reported as antibiofilms so that they have the potential as an alternative mouthwash. This study aims to analyzed the antibiofilm activity of ethanolic extract of shallot peel (EESP) with a concentration of 6.25%; 12.5%; 25%; 50%; and 100% in inhibiting the formation of S. aureus biofilms. Methods: This research was an experimental study. Extracts were prepared using 96% ethanol maceration method by immersion for 15 days. The positive control in this study was CHG 0.2% and the negative control was DMSO 1%. Inhibition of biofilm formation was measured using a Microtiter Plate Assay (MPA) with a wavelength of 595 nm. The data from the MPA readings were analyzed statistically by the Kruskal-Wallis test and the Mann-Whitney post hoc test. Results: The highest percentage of biofilm inhibition was in EESP 25% (92.86+1.45%) but this result was significantly lower than the positive control group (94.74+0.56%) (p<0.05). There was no significant difference (p<0.05) between the percentage of inhibition of biofilm formation of EESP 12.5% (92.74+0.98%) and EESP 25% statistically. Conclusion: The most effective inhibition of biofilm formation by EESP against S. aureus biofilms was found at a concentration of 12.5%.Keywords: Allium cepa L, biofilm, shallot peel, Staphylococcus aureus
EFFECT OF TORCH GINGER EXTRACT (Etlingera elatior) ON THE ERADICATION OF Aggregatibacter actinomycetemcomitans BIOFILM ANS CAUSES AGGRESSIVE PERIODONTITIS Meylida Ichsyani; Haris Budi Widodo; Rifda Naufalin; Aisha Tiara Dewi; Adninda Rimawati; Devi Anisya Putri; Novita Dwi Lokasari
Mandala Of Health Vol 13 No 2 (2021): Mandala Of Health
Publisher : Fakultas Kedokteran Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mandala.2021.13.2.8745

Abstract

Aggregatibacter actinomycetemcomitans is an oral microflora that forms biofilms and is the main cause of aggressive periodontitis. Long-term mouthwash therapy can cause mucositis and even oral cancer. The ethanol extract of torch ginger (Etlingera elatior) is reported to have analgesic, anti-inflammatory, and antibacterial activities, so it can be developed as an alternative to prevention or adjuvant therapy for aggressive periodontitis. The aim of this study was to determine the effect of the ethanol extract of the torch ginger plant on the biofilm degradation of A. actinomycetemcomitans. The study used ethanol extracts of torch ginger flowers, leaves, and stems at concentrations of 1.56 mg/mL, 3.125 mg/mL, 6.25 mg/mL, 12.5 mg/mL, 25 mg/mL, and 50 mg/mL. CHX 0.2% mouthwash was used as a positive control, while 1% DMSO was used as a negative control. The biofilm degradation test was carried out using a microtiter plate assay with 1% crystal violet staining at a wavelength of 450 nm. The results showed that there was significant activity against biofilm degradation of A. actinomycetemcomitans in the administration of an ethanol extract of torch ginger flowers, leaves, and stems compared to the negative control (p<0.05). The optimum concentrations of biofilm degradation by extracts of flowers, leaves, and stems were 25 mg/mL (85.54%), 25 mg/mL (84.43%), and 50 mg/mL (72.10%), respectively. MBEC50 values of flowers are 4.82 mg/mL, MBEC50 leaves are 5.96 mg/mL, and MBEC50 stems are 10.15 mg/mL. The conclusion of this study is that there is an effect of giving an ethanol extract of torch ginger flowers, leaves, and stems on the biofilm degradation of A. actinomycetemcomitans bacteria.
Antimicrobial photodynamic therapy with erythrosine photosensitizer against immune response in chronic periodontitis model Rochmawati, Mutia; Kusuma, Maulidina Raihan; Maziyyah, Faiz; Naim, Cantika Nadrotan; Prihastuti, Christiana Cahyani; Satrio, Rinawati; Laksitasari, Anindita; Sari, Dwi Nur Indah; Ichsyani, Meylida
Majalah Kedokteran Gigi Indonesia Vol 9, No 2 (2023): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.77084

Abstract

Chronic periodontitis is a progressive inflammatory disease of the supporting tissues of the teeth caused by dental plaque bacteria with a clinical sign of periodontal pockets. A Gram-negative bacterium that can trigger this inflammatory disease is Porphyromonas gingivalis. Antimicrobial photodynamic therapy with blue LED light irradiation and photosensitizer erythrosine can reduce the survival rate of P. gingivalis. This study aimed to determine the effects of antimicrobial photodynamic therapy (APDT) exposure with blue LED light irradiation and PS erythrosine on the number of macrophages, lymphocytes, and gingival fibroblasts in gingival tissue of Sprague Dawley rats as chronic periodontitis models. This study used a posttest-only control group design to examine 27 Sprague Dawley rats which were divided into P group (healthy rats), N group (untreated chronic periodontitis rats), and PDT groups (chronic periodontitis model given 1 mg/ml PS erythrosine and irradiated with blue LED light for 60 seconds). Cell observation of histologic preparations of rat gingival tissue with hematoxylin-eosin (H&E) staining was carried out on the 1st, 3rd, and 5th days. Histological preparations of gingival tissue with H&E staining was carried out on the 1st, 3rd, and 5th days. Statistical analysis used a one-way ANOVA and the Kruskal-Wallis test, continued with LSD and the Mann-Whitney post-hoc tests. Results showed significant difference in the mean of macrophages in the PDT group compared to the untreated chronic periodontitis group on the 1st, 3rd, and 5th days (p < 0.05). The mean lymphocyte in the PDT group was significantly different from the untreated chronic periodontitis group on the 1st, 3rd, and 5th days (p < 0.05), and significantly lower than that in the healthy group (p < 0.05) but only on the 3rd and 5th days. The mean fibroblast in the PDT group was significantly different compared to the untreated chronic periodontitis group on the 1st, 3rd, and 5th days (p < 0.05). In conclusion, there were significant differences in the number of macrophages, lymphocytes, and fibroblasts in a chronic periodontitis rat model after treatment with APDT exposure with blue LED and erythrosine photosensitizer.
Potensi larutan teh hijau celup sebagai alternatif Hank’s Balanced Salt Solution mempertahankan viabilitas sel ligamen periodontal gigi avulsi: studi in vitro Zahratuljannah, Reshaina Dewi Azizah; Widodo, A. Haris Budi; Triani, Maulina; Ichsyani, Meylida; Rochmawati, Mutia
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 36, No 2 (2024): Agustus 2024
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkg.v36i2.55391

Abstract

ABSTRAKPendahuluan: Avulsi gigi terjadi ketika gigi terlepas sepenuhnya dari soket alveolar akibat trauma. Perawatan awal yang dilakukan pada gigi avulsi/replantasi, yaitu menanamkan kembali gigi ke dalam soket dengan segera. Prognosis kesuksesan replantasi gigi sangat bergantung pada viabilitas sel ligamen periodontal sehingga memerlukan media penyimpanan yang sesuai dan manajemen waktu yang tepat. Penelitian bertujuan menganalisis potensi larutan teh hijau celup sebagai alternatif penggunaan Hank’s Balanced Salt Solution (HBSS) pada viabilitas sel ligamen periodontal pada gigi. Metode: Penelitian dilakukan secara eksperimental laboratoris in vitro menggunakan sampel sel ligamen periodontal pada 32 gigi insisivus maksila tikus Wistar. Sampel dibagi menjadi 8 kelompok, yaitu kelompok yang direndam dalam larutan teh hijau celup dan kelompok kontrol positif pada HBSS selama 1, 3, 6, dan 24 jam. Metode analisis penghitungan persentase viabilitas sel ligamen periodontal dengan pewarnaan eksklusi trypan blue dibawah mikroskop dengan perbesaran 100x. Analisis statistik menggunakan one way ANOVA untuk membandingkan persentase antar waktu pada teh hijau celup dan independent t-test untuk membandingkan teh hijau celup dan HBSS pada setiap waktu. Hasil: Hasil one way ANOVA p>0,05 yang menandakan bahwa tidak terdapat perbedaan signifikan antar perendaman dalam larutan teh hijau celup. Hasil independent t-test pada jam ke 1 dan 3 p>0,05 yang menandakan tidak terdapat perbedaan signifikan dan pada jam ke 6 dan 24 p<0,05 menandakan adanya perbedaan signifikan antara perendaman dalam larutan teh hijau celup dan larutan kontrol positif HBSS. Simpulan: Larutan teh hijau celup dapat menjadi alternatif HBSS dalam mempertahankan viabilitas sel ligamen periodontal gigi avulsi.Potential of green tea bag solution as an alternative to Hank’s Balanced Salt Solution in maintaining periodontal ligament cell viability in Wistar rat avulsed teeth: in vitro study Introduction: Tooth avulsion occurs when the tooth is completely separated from the alveolar socket due to trauma. The initial treatment for an avulsed tooth is replantation, namely immediately implanting the tooth back into the socket. The prognosis for successful tooth replantation is highly dependent on the viability of periodontal ligament cells. Use of appropriate storage media and proper time management are critical to preserving periodontal ligament cells and the likelihood of successful replantation. This study aims to analyze potential of a green tea bag solution as an alternative to using Hank’s Balanced Salt Solution (HBSS) on periodontal ligament cell viability in avulsed teeth. Methods: The research was carried out experimentally in vitro using periodontal ligament cell samples from 32 maxillary incisors of Wistar rats. The samples were divided into 8 groups, namely the group soaked in green tea bag solution and the positive control group in HBSS for 1, 3, 6, and 24 hours. The analytical method is to calculate the percentage of periodontal ligament cell viability using trypan blue exclusion staining under a microscope with 100x magnification. Statistical analysis used one way ANOVA to compare percentages between times of bagged green tea and independent t-test to compare bagged green tea and HBSS at each time. Results: The results of one way ANOVA were p>0.05 which indicated that there was no significant difference between immersion in green tea bag solution. The results of the independent t-test at the 1st and 3rd hours were p>0.05 which indicated there was no significant difference and at the 6th and 24th hours p<0.05 which indicated there was a significant difference between immersion in the green tea bag solution and HBSS as the positive control solution. Conclusion: Green tea bag solution can be an alternative to HBSS in maintaining periodontal ligament cell viability in avulsed teeth.
Antibiofilm Activity of Secang Wood (Caesalpinia sappan) Ethanol Extract Against Cariogenic Streptococcus sanguinis HAIBAR, FERINA AYU MAHARANI; Prihastuti, Christiana Cahyani; Ichsyani, Meylida
Journal of Indonesian Dental Association Vol 8 No 1 (2025): May
Publisher : Indonesian Dental Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32793/jida.v8i1.1278

Abstract

Streptococcus sanguinis merupakan bakteri pioneer colonizers yang berperan dalam perkembangan biofilm penyebab penyakit karies gigi. Ekstrak etanol kayu secang memiliki potensi antibiofilm yang dapat dikembangkan sebagai terapi alternatif obat kumur untuk pencegahan karies gigi. Penelitian bertujuan untuk mengetahui aktivitas antibiofilm ekstrak etanol kayu secang (Caesalpinia sappan L.) terhadap Streptococcus sanguinis penyebab karies gigi. Aktivitas antibiofilm menggunakan dua uji, uji pertama degradasi biofilm dan uji kedua penghambatan biofilm lanjutan dengan MtP Assay dan pewarnaan kristal violet 1% yang densitas optiknya dibaca pada panjang gelombang 620 nm. Data hasil degradasi penelitian dianalisis menggunakan One-Way ANOVA dan post-hoc LSD. Hasil menunjukkan terdapat perbedaan bermakna seluruh konsentrasi ekstrak dengan kontrol negatif. Konsentrasi efektif degradasi biofilm yaitu konsentrasi 1,56 mg/mL dan konsentrasi efektifpenghambatan pertumbuhan biofilm pada konsentrasi 0,39 mg/mL. Simpulan dari penelitian ini adalah terdapat aktivitas antibiofilm ekstrak etanol kayu secang terhadap Streptococcus sanguinis penyebab karies gigi.
Aktivitas antibiofilm ekstrak etanol daun saga (Abrus precatorius) terhadap biofilm bakteri Aggregatibacter actinomycetemcomitans pada pengujian menggunakan MtPB assay: experimental laboratoris Putri, Annisa Liontyn Adies; Ichsyani, Meylida; Prihastuti, Christiana Cahyani
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 36, No 3 (2024): Desember 2024
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkg.v36i3.57014

Abstract

Pendahuluan: Aggregatibacter actinomycetemcomitans merupakan flora normal pembentuk biofilm di rongga mulut. Peningkatan jumlah bakteri ini secara berlebihan dapat menyebabkan terjadinya periodontitis agresif dengan prevalensi mencapai 90% sehingga berperan penting dalam peningkatan keparahan periodontitis agresif secara cepat. Daun saga (Abrus precatorius) memiliki aktivitas farmakologis seperti antitumor, antidiabetik, antihelmintes, dan antimikroba dengan kandungan fitokimia berupa tanin, saponin, flavonoid, steroid, dan alkaloid. Penelitian bertujuan untuk menganalisis aktivitas antibiofilm ekstrak etanol daun saga terhadap biofilm                            A. actinomycetemcomitans berfokus pada aktivitas degradasi dan penghambatan pembentukan biofilm. Metode: Penelitian dilakukan dengan metode MtPB menggunakan pewarna kristal violet. Ekstrak diuji pada berbagai konsentrasi bertingkat yaitu 0,39, 0,78, 1,56, 3,125, 6,25, 12,5, 25, dan 50 (mg/mL) yang dibandingkan terhadap kontrol negatif DMSO 1% serta kontrol positif amoksisilin+metronidazol 30 µg/mL. Data yang didapatkan melalui desain penelitian posttest only control group design kemudian dianalisis menggunakan analisis statistik one-way ANOVA dan analisis regresi linier Hasil: Hasil uji degradasi dan penghambatan pembentukan biofilm menunjukkan aktivitas pada seluruh kelompok perlakuan dibandingkan dengan kontrol negatif (p≤0,05). Ekstrak etanol daun saga konsentrasi 25 mg/mL memiliki kemampuan degradasi sebesar 76,13% serta sudah mampu menyamai kemampuan degradasi kelompok kontrol positif (p>0,05) dan mulai dari konsentrasi 1,56 mg/mL sudah menghambat biofilm mulai dari 37,52% sehingga mampu menyamai dan konsentrasi melebihi kemampuan penghambatan pembentukan biofilm A. actinomycetemcomitans pada kelompok kontrol positif. Nilai Minimum Biofilm Eradication Concentration (MBEC50) terdapat pada konsentrasi 4,44 mg/mL sedangkan nilai Minimum Biofilm Inhibitory Concentration (MBIC50) pada konsentrasi 2,96 mg/mL. Simpulan: ekstrak etanol daun saga memiliki aktivitas antibiofilm terhadap bakteri A. actinomycetemcomitans.Antibiofilm activity of ethanol extract of saga leaf (Abrus precatorius) on Aggregatibacter actinomycetemcomitans Biofilm: experimental laboratorisIntroduction: Aggregatibacter actinomycetemcomitans is a normal flora that forms biofilms in the oral cavity. An excessive increase in the number of these bacteria can cause inflammation of the periodontal tissue called periodontitis. A. actinomycetemcomitans has the main virulence of leukotoxin which can weaken the immune cell response to pathogenic bacteria with a prevalence in aggressive periodontitis reaching 90% so it plays an important role in the rapid increase in the severity of aggressive periodontitis. Saga leaves (Abrus precatorius) have pharmacological activities such as antitumor, antidiabetic, antihelminthic, and antimicrobial with phytochemical content in the form of tannins, saponins, flavonoids, steroids, and alkaloids. The research aims to analyze the antibiofilm activity of ethanol extract of saga leaves against A. actinomycetemcomitans biofilms by looking at the degradation activity and inhibition of biofilm formation. Method: The research was carried out using the MtPB method using crystal violet dye. The extract was tested at various graded concentrations, namely 0.39, 0.78, 1.56, 3.125, 6.25, 12.5, 25, and 50 (mg/mL) which were compared to the negative control 1% DMSO and the positive control amoxicillin +metronidazole 30 µg/mL. Data obtained through the posttest only control group design were then analyzed using one-way ANOVA statistical analysis and linear regression. Results: The results of the degradation and inhibition of biofilm formation tests showed activity in all treatment groups compared to the negative control (p≤0.05). The ethanol extract of saga leaves at a concentration of 25 mg/mL was able to match the degradation ability of the positive control group (p>0.05) and starting from a concentration of 1.56 mg/mL was able to match and exceed the ability to inhibit A. actinomycetemcomitans biofilm formation in the positive control group. The MBEC50 value is found at a concentration of 4.44 mg/mL while the MBIC50 value is at 2.96 mg/mL. Conclusion: ethanol extract of saga leaves has anti-biofilm activity against the bacteria A. actinomycetemcomitans.
INHIBITION OF Asystasia gangetica ETHANOLIC EXTRACT ON Porphyromonas gingivalis AND Fusobacterium nucleatum BIOFILM FORMATION Ichsyani, Meylida; Laksitasari, Anindita; Supriyati, Supriyati; Sari, Dwi Nur Indah; Wahyudin, Wahyudin
Mandala Of Health Vol 18 No 1 (2025): Mandala of Health: A scientific Journal
Publisher : Fakultas Kedokteran Universitas Jenderal Soedirman

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Abstract

Periodontal pathogens Porphyromonas gingivalis and Fusobacterium nucleatum develop a biofilm on the surface of the dental apex, causing inflammation in the teeth' supporting tissues. Asystasia gangetica has been observed for its analgesic, anti-inflammatory, and antimicrobial effect. The ethanolic extract of A. gangetica may be developed as a root canal sterilization dressing due to its benefits. This study aims to determine the inhibitory effect of ethanolic extract of A. agangetica on P. gingivalis and F. nucleatum from forming biofilm. Antibacterial activity was determined using Kirby-Bauer disk diffusion. The inhibition of biofilm formation was evaluated using microtiter plate biofilm assay with various concentrations of extract. Base on the classification of inhibition zone, ethanolic extract of A. gangetica had moderate to strong antibacterial effects on P. gingivalis at 12.5-50 mg/mL, and moderate effect on F. nucleatum at 25-50 mg/mL. Results also showed that the extract at 50 mg/mL, inhibited biofilm formation by more than 72% against P. gingivalis (p<0.05) and up to 75% against F. nucleatum (p<0.05). The value of MBIC50 was determined at 5.19 mg/mL and 7.44 mg/mL, respectively. Ethanolic extract of Asystasia gangetica has a potential inhibitory effect on P. gingivalis and F. nucleatum biofilm formation. Studies suggest more detailed approaches to a better understanding of the interactions between lead compounds and bacterial cells within the biofilm structure.
Antifungal Activity Test Ethanol Extract Of Awar-Awar Leaves (Ficus septica Burm. F.) to Candida tropicalis As The Cause of Oral Candidiasis In Vitro Bagus, Zahran Uzla Attaqi; Anjarwati, Dwi Utami; Djati, Fanni Kusuma; Ichsyani, Meylida
Jurnal Medali Vol 7, No 2 (2025): Media Dental Intelektual Agustus 2025
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/medali.7.2.136-144

Abstract

Background: Oral candidiasis is an infection in the oral cavity caused by Candida tropicalis, a non-Candida albicans (NCAC) species with the highest virulence. The first-line treatment for oral candidiasis is nystatin; however, it may cause side effects. One alternative treatment for oral candidiasis is the use of Ficus septica Burm. F. leaves, which contain active compounds such as saponins, tannins, flavonoids, and triterpenoids, all of which have potential antifungal properties. This study aims to evaluate the antifungal activity ethanol extract of awar-awar leaves to Candida tropicalis as the cause of Oral Candidiasis in vitro. Method: This experimental laboratory study used a posttest-only control group design, comparing the treatment groups with ethanol extract of Ficus septica burm. F leaves at concentrations of 4%, 8%, 16%, and 32% with a positive control group (nystatin) and a negative control group (Dimethyl sulfoxide). Antifungal activity was tested using the microdilution broth method to determine the Minimum Inhibitory Concentration (MIC) and the spread plate method to determine the Minimum Bactericidal Concentration (MBC). Colony growth of C. tropicalis was measured using the Total Plate Count (TPC) method, followed by calculating the inhibition percentage. Result: The analysis of this study showed that the MIC of Ficus septica Burm F leaf extract was at a concentration of 32%, and the MBC of the extract was also at a concentration of 32%, with a 100% inhibition percentage. Significant differences (p ≤ 0.05) were found between the concentrations of the ethanol extract of Ficus septica leaves.Conclussion: The conclusion of this study is that the ethanol extract of Ficus septica Burm. F leaves has antifungal activity in inhibiting the growth of C. tropicalis, the cause of oral candidiasis, with the most effective concentration being 32% with 100% inhibition.
Co-Authors -, Oedjijono A Haris Budi Widodo A. Haris Budi Widodo Adninda Rimawati Aisha Tiara Dewi Alya Ghina Rosyada Anindita Laksitasari Aris Aji Kurniawan Bagus, Zahran Uzla Attaqi Christiana Cahyani Prihastuti Devi Anisya Putri Dewi, Aisha Tiara Dwi Nur Indah Sari Dwi Nur Indah Sari, Dwi Nur Indah Dwi Utami Anjarwati Eka Prasasti Nur Rachmani Fadli Ashar Fanni Kusuma Djati HAIBAR, FERINA AYU MAHARANI Haris Budi Widodo Kusuma, Maulidina Raihan Laksitasari, Anindita Maziyyah, Faiz Multri, Violentri Mutia Rochmawati Nadira, Nimas Rahma Naim, Cantika Nadrotan Novita Dwi Lokasari Peramiarti, IDSAP Prihastuti, Christiana Cahyani Putri, Annisa Liontyn Adies Putri, Devi Anisya Rakmawati Rakmawati Restian Febi Andini Rifda Naufalin Rinawati Satrio Riski Amalia Hidayah Salwa Az-Zahra Satrio, Rinawati Setiawati Setiawati Siregar, Raja Ronaldo Solihin, Bella Ayuni Adhianti Supriyati . Supriyati Supriyati Tanjung, Nurul Hasanah Triani, Maulina Wahyudin Wahyudin Widodo, A Haris Budi Zahratuljannah, Reshaina Dewi Azizah