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All Journal Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research)
Kuntum Khoirani
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Agriculture, Biological Sciences & Forestry Veterinary

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Detection of Ampicillin Resistance Encoding Gene of Escherichia coli from Chickens in Bandung and Purwakarta Kuntum Khoirani; Agustin Indrawati; Surachmi Setiyaningsih
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 3 No. 1, JANUARY 2019
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v3i1.6134

Abstract

The purpose of this study was to test the resistance and to detect antibiotic resistence encoding gene in E. coli bacteria from chickens in Bandung and Purwakarta livestock. 18 E. coli isolates were tested for antibiotics resistance using the disk diffusion method. Isolates that were categorized as resistant and intermediate to antibiotics, then polymerase chain reaction was utilized to detect the resistent coding gene. The test results showed that all E. coli isolates from chickens in Bandung and Purwakarta were resistant to ampicillin (100%). E. coli isolates were still sensitive to chloramphenicol (11.1%) and gentamicin (22.2%). The gene encoding for ampC resistance from the test were in the amount of 77.7%. Sensitivity test results and detection of resistance coding gene showed that almost all isolates were resistant to ampicillin antibiotics and E. coli isolates were still sensitive to chlorampenicol and gentamicin. 
Detection of Brucellosis in Bali Cattle (Bos Sondaicus ) in Bolo District, Bima Regency Karni, Ine; Lukman HY; Kuntum Khoirani; Burhan; Oscar Yanuariyanto
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 7, No 1, JANUARY 2023
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v7i1.21279

Abstract

This study aims to determine the incidence of Brucellosis in Bali cattle in Bolo district, Bima regency, based on serological tests (seroprevalence). The sample used in this study was cattle serum. Serum samples were obtained from 70 Bali cattle aged over one year. The detection method, namely the Rose Bengal Test, detects the incidence of Brucellosis. Samples in the form of serum are reacted with Brucella sp. antigen to see antibodies in the serum. The working principle of the Rose Bengal Test is to see whether there is agglutination or not. The results of this study will be an indicator to determine the presence or absence of Brucellosis in Bali cattle in Bolo district, Bima regency. Then the monitoring and control can be established by relevant stakeholders, especially the Livestock and Animal Health Service of Bima Regency. The data obtained from serum samples that describe the positive or negative for Brucellosis is presented descriptively by explaining each sample origin's seropositive and seronegative results. The results showed that in all samples from Bali cattle in the District of Bolo, as many as 70 samples reacted negatively to the Rose Bengal Test. This result indicates that the cattle on Sumbawa Island are not infected with Brucellosis. Keywords: Brucellosis, Reproductive Disorders, Bali Cattle, Rose Bengal Test
Co-Authors Agustin Indrawati Burhan Ine Karni Lukman HY Oscar Yanuariyanto Surachmi Setiyaningsih