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GEJALA KLINIS DAN PATOLOGI ANATOMI PASCA INFEKSI Campylobacter jejuni PADA AYAM BROILER [Clinical Symptoms and Pathology Anatomy due to Infection Campylobacter jejuni on Broilers] Andriani, Andriani; Sudarwanto, Mirnawati; Setiyaningsih, Surachmi; Kusumaningrum, Harsi Dewantari; Pisestyani, Herwin
BERITA BIOLOGI Vol 11, No 3 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Campylobacter spp. is a bacteria foodborne zoonotic agent that can infect humans and animals, especially birds. This bacteria can cause important problems in the public health since the beginning at the farm level, the level of retail and on the table. Poultry meat is the main source of contamination because the digestive tract of poultry is the predilection of Campylobacter jejuni. During this infection C. jejuni in poultry did not show typical clinical symptoms, making detection quite difficult to diagnose this disease at the farm level. Incidence infection of Campylobacter sp. in developed countries had 5-90%. Currently poultry production and consumption are predicted to still increase due to a high demand for low price protein. This research is conducted to determine the clinical and pathologic anatomy due to an infection of C. jejuni on chicken digestive tract. A total of 105-day-old chickens are divided into 3 groups, each consisting of 35 individuals. Group A was the control group (not infected) group B (infected with C. jejuni ATCC 33291), and group C (infected with field isolate of C. jejuni/C1). Infection of the chicken is done 0.5 ml peroral suspension concentration of104cfu/ml. Anova Duncan is used to analyze parameters of body weight, increase weight gain, feed consumption, FCR values. Kurskall Walis method used to analyze the parameters non-parametric form of the score changes in the macroscopic and microscopic intestinal and liver. Infection of C. jejuni changes the pathology anatomy of intestine i.e. hyperemia, enteritis and enteritis catharalis haemorrhagic. A microscopic change of intestinal is oedema,haemorrhage and infiltration of inflammatory cell. Changes of pathology anatomy liver are colour smeary, congesti, pale, fragile and necrotic. Microscopic changes of liver are degeneration and focal hepatic necrosis. Group observation infected by C. jejuni caused chicken cannot reach maximum body weight and can be detrimental to farmers because of increase of the value of the feed conversion ratio (FCR).

ISOLASI Campylobacter DARI KARKAS AYAM MENGGUNAKAN METODE KONVENSIONAL DAN POLYMERASE CHAIN REACTIONS [Isolation of Campylobacter from Poultry Carcasses using Conventional and Polymerase Chain Reaction Methods] . Andriani; Mirnawati Sudarwanto; Surachmi Setiyaningsih; Harsi Dewantari Kusumaningrum
Jurnal Teknologi dan Industri Pangan Vol. 24 No. 1 (2013): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

ABSTRACT Campylobacter jejuni and Campylobacter coli are two spesies of Campylobacter sp. frequently found as pathogenic bacteria causing human gastrointestinal infections. Contaminated chicken carcasses have been reported as the source of human campylobacteriosis. In this study, Campylobacter were isolated from chicken carcasses sold in traditional markets and supermarkets. In traditional markets, chicken carcasses are sold without proper packaging or in an open space and stored at room temperature (25-30°C) for prolonged period allowing pathogenic bacteria to grow. While at supermarkets, chicken carcasses are openly displayed or enclosed in plastic wrappings and stored in a refrigerator (4-8°C). A total of 298 samples of chicken carcasses from traditional markets and supermarkets in the area of DKI Jakarta, West Java (Bogor and Sukabumi) and Central Java (Kudus and Demak) were collected. Isolation and identification using conventional and Polymerase Chain Reactions (PCR) methods were done to determine the prevalence of C. jejuni and C. coli contamination in poultry. The results showed that chicken carcasses sold in the sampling area, both traditional markets and supermarkets, were contaminated with C. jejuni and C. coli. The contamination rate of Campylobacter sp. in chicken carcasses sold in supermarkets, were 14.1% by conventional methods and 29.5% by PCR. This was higher than those in traditional markets, i.e. 5.7 and 12.1%, respectively. It is also confirmed that the prevalence for contamination of C. jejuni was higher than C. coli in 298 samples, i.e. 16.1% and 3.7% by conventional method and 23.5% and 18.1% by PCR method respectively. Keywords: Campylobacter coli, Campylobacter jejuni, poultry carcasses, supermarket, traditional market

Penentuan Patotipe Molekuler Virus Newcastle Disease: Isolat Lapang di Tiga Wilayah Kabupaten Jawa Timur Erin Kurnianingtyas; Surachmi Setiyaningsih; Agustin Indrawati
Acta VETERINARIA Indonesiana Vol. 5 No. 1 (2017): Januari 2017
Publisher : IPB University

Tujuan penelitian ini adalah mengkaji keberadaan dan karakteristik molekuler virus newcastle disease (VND) di tiga wilayah Kabupaten di Jawa Timur. Sampel usapan kloaka diambil dari 289 ekor unggas (262 ekor ayam dan 27 ekor bebek) pekarangan, pedagang di pasar unggas hidup, peternak, dan pengepul di wilayah Kabupaten Probolinggo, Situbondo, dan Bondowoso. Sampel usap kloaka ditumbuhkan pada telur ayam berembrio (TAB) dan deteksi virus dengan Real Time Reverse Transcription Polymerase Chain Reaction (rRT-PCR) matrix (M). Patogenisitas virus ditentukan melalui rRT-PCR Fusion (F) dan sekuen gen F. Sejumlah delapan isolat lapang VND yang didapat, semuanya mempunyai afinitas lebih tinggi dengan serum Komarov dibandingkan dengan B1. Hal tersebut sesuai dengan reaksi positif yang ditunjukkan oleh rRT-PCR F. Analisis sekuen nukleotida menegaskan adanya motif asam amino multibasic pada cleavage site protein F, terbukti dari enam isolat asal ayam dan satu isolat asal bebek memiliki motif 112RRQKRF117, sedangkan satu isolat asal bebek lainnya mempunyai motif112RRRKRF117.

Prevalensi dan Karakterisasi Molekuler Infectious Myonecrosis Virus (IMNV) di Sentra Budidaya Udang Vaname (Litopenaeus Vannamei) Propinsi Banten Rona Choiruz Zaujat; Surachmi Setiyaningsih; Angela Mariana Lusiastuti
Acta VETERINARIA Indonesiana Vol. 4 No. 2 (2016): Juli 2016
Publisher : IPB University

Infectious Myonecrosis Virus (IMNV) adalah virus yang umum menyerang udang putih (Litopenaeus vannamei) dalam industri budidaya udang di dunia. Di Indonesia, penyakit myonecrosis pertama kali diketahui terjadi pada udang putih dari pertambakan di Situbondo, Jawa Timur, pada tahun 2006 dengan prevalensi 11.11% dan gejala klinis serupa dengan kejadian wabah myonecrosis di Brazil pada tahun 2002. Penelitian ini bertujuan untuk mengetahui prevalensi dan karakteristik molekuler IMNV di sentra budidaya udang vaname di Propinsi Banten. Sampel udang dikumpulkan selama periode Maret hingga Juni 2015. Sebanyak 24 sampel diperoleh dari 24 area pertambakan aktif kemudian di-pool dan diuji menggunakan metode Real Time Polymerase Chain Reaction (PCR). Hasil uji menunjukkan bahwa prevalensi IMNV secara keseluruhan di Propinsi Banten adalah sebesar 33,3% dengan rincian: Kota Serang 0%; Kabupaten Serang 0%; Kabupaten Tangerang 14,3% dan Kabupaten Pandeglang 100%. Analisis perbandingan genom IMNV pada ORF1parsial menunjukkan bahwa isolat lapang Banten, isolat Indonesia dan Brazil memiliki persentase kemiripan 97,4-100%. Analisis sekuens asam amino menunjukkan persentase kemiripan 97,6-100%. Analisis fi logenetik sekuens nukleotida menunjukkan bahwa telah terjadi diversifikasi genetik antara IMNV Indonesia dan Brazil dan antar isolat Indonesia sendiri.

Application of Multiplex PCR Assay for Campylobacter fetus Sub sp. Detection and Differentiation in Bovine Preputial Wash Seruni Agistiana; Surachmi Setiyaningsih; Agustin Indrawati
Buletin Peternakan Vol 42, No 1 (2018): BULETIN PETERNAKAN VOL. 42 (1) FEBRUARY 2018
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v42i1.26114

The aim of this research was to evaluate the detection and differentiation of C. fetus subspecies through multiplex PCR (mPCR) method from the bovine preputial wash. The evaluation was done by comparing 2 extraction methods, which was by commercial kit extraction and boiling method, and also by measuring the effect of Transport Enrichment Media (TEM) addition as bacterial enrichment. Spiked bovine preputial washes with 2 extraction methods (commercial kit and boiling), with and without TEM addition were used as a sample. The results showed that the highest sensitivity/specificity was by boiling extraction without TEM addition, which reached 83.3%/100% for all or grouped sample. The results also supported by Poisson regression analysis which showed the comparison between sample with and without TEM addition which had 1.3 Incidence Rate Ratio (IRR). The obtained IRR score indicated that sample without TEM addition had 30% better result compared to the sample with TEM addition. Furthermore, a comparison between commercial kit and boiling extraction showed no significant difference (1.029 IRR). The research concluded that boiling extraction without TEM addition is the most accurate, fast and economical extraction method regarding its high sensitivity and specificity value, with less than 24 hours testing time and without any commercial kit requirements. However, a validation and verification with field sample were still needed before applied in the laboratory.

Metode Direct Polymerase Chain Reaction untuk Melacak Campylobacter sp. pada Daging Ayam (DIRECT POLYMERASE CHAIN REACTION METHOD FOR DETECTION CAMPYLOBACTER SP. OF POULTRY MEAT) Andriani .; Mirnawati Sudarwanto; Surachmi Setiyaningsih; Harsi Dewantari Kusumaningrum
Jurnal Veteriner Vol 14 No 1 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (193.705 KB)

Campylobacter sp. is the most commonly reported as agent of foodborne zoonosis causing acutegastroenteritis in humans. Poultry meat is considered as a major source of C. jejuni infection in human.The conventional methods for detecting foodborne bacteria is time-consuming which rely on the of thebacteria in culture media, followed by biochemical identification. In this study polymerase chain reaction(PCR) technique was used for rapid identification of the pathogenic Campylobacter sp. The samples usedwere 298 chicken carcass with sold in supermarkets and traditional markets, and were carried out inaccordance the isolation protocol ISO/ DIS 10272-1994. Identification was performed using biochemicalAPI Campy. The direct PCR (DPCR) assay with two sets of primers was employed for isolation andidentification of C. jejuni and C. coli. The result of the isolation and identification both by conventional orPCR methods showed that chicken carcasses both from supermarket and traditional market werecontaminated with C. jejuni and or C. coli. Prevalence of Campylobacter sp. contamination in chicken meatwas higher by DPCR (62.6%) than by conventional (19.8%), indicating that DPCR technique was moresensitive than conventional method with detection limit for C. jejuni was103 cfu/ml.

Pengembangan Enzyme-Linked Immunosorbent Assay Paratuberkulosis dengan Antigen Protoplasmik Mycobacterium avium Subspecies Paratuberculosis Isolat Lapang (DEVELOPMENT OF PARATUBERCULOSIS ENZYME-LINKED IMMUNO-SORBENT ASSAY WITH PROTOPLASMIC ANTIGEN OF MYCO Rahmat Setya Adji; I Wayan Teguh Wawan; Denny Widaya Lukman; Surachmi Setiyaningsih
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (130.702 KB)

Enzyme-linked immunosorbent assay (ELISA) is a serological test method most widely used for thediagnosis of paratuberculosis, because it has a better sensitivity compared to other serological test.Protoplasmic antigen (PPA) or cellular extract is still the main choice for the diagnosis of paratuberkulosisdevelopment. The aim of research was to use the PPA Mycobacterium avium subspeciesparatuberculosis(MAP) field isolates for the development of paratuberculosis ELISA (ELISA PPA-L). As many as 322cattle sera (300 negative and 22 positive) were tested using this method and compared with IDEXXcommercial kit. The sensitivity and specificity of ELISA PPA-L test results were 68.18% and 97.0%,whereas for the IDEXX kit were 63.64% and 97.33%respectively. ELISA PPA-L had higher sensitivity andlower specificity compared to the IDEXX commercial kit. ELISA test using protoplasmic antigen of MAPfield isolates has good ability for paratuberculosis serological test and can be used for screening test of thedisease in Indonesia.

STUDI HISTOPATOLOGI PADA LIMPA DAN BURSA FABRICIOUS AYAM NEWCASTLE DISEASE DARI KASUS LAPANG HISTOPATHOLOGY (STUDIES ON SPLEEN AND BURSA OF FABRICIUS OF NEWCASTLE DISEASE CHICKHENS FROM FIELD CASE) Etriwati Etriwati; Ekowati Handharyani; Surachmi Setiyaningsih
Jurnal Veteriner Vol 18 No 4 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Newcastle Disease is a highly contagious and very virulent avian disease in all of poultry. The aims of this study were to determine the lesions and distribution of Newcastle disease virus on spleen and bursa of Fabricous of Newcastle disease chickhens from field cases. The sample used in this study were the spleen and bursa Fabricious organ of ten cases of suspect Newcastle disease. Haematoxylin and eosin staining examination was performed determine of cell morphology and distribution of the virus by immunohistochemical staining. The distribution of the lesions and the degree of reaction were determined by the category immunopositive mild, moderate and severe. Gross lesion on spleen, including swollen, fragile and necrotic multifocal. Histopathological lesions showed hyperemia/congestion and depletion of lymphoid cells. Immunopositive reaction found in lymphoid cells of red pulp and lymphoid cells in lymphoid follicles. Bursa of Fabricious showed atrophy and hyperemia/congestion with histopatological lesions hemorrhages, lymphoid cells in lymphoid follicles undergo necrosis and depletion of lymphoid follicles. Immunopositif reaction was observed in lymphoid cells in the mantle zone of lymphoid follicles. Newcastle disease from field cases cause damage to the spleen and bursa of Fabricius with degrees lesions and virus distribution in the organs are severe.

GEJALA KLINIS DAN PATOLOGI ANATOMI PASCA INFEKSI Campylobacter jejuni PADA AYAM BROILER Andriani Andriani; Mirnawati Sudarwanto; Surachmi Setiyaningsih; Harsi Dewantari Kusumaningrum; Herwin Pisestyani
BERITA BIOLOGI Vol 11, No 3 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v11i3.498

Campylobacter spp. is a bacteria foodborne zoonotic agent that can infect humans and animals, especially birds. This bacteria can cause important problems in the public health since the beginning at the farm level, the level of retail and on the table. Poultry meat is the main source of contamination because the digestive tract of poultry is the predilection of Campylobacter jejuni. During this infection C. jejuni in poultry did not show typical clinical symptoms, making detection quite difficult to diagnose this disease at the farm level. Incidence infection of Campylobacter sp. in developed countries had 5-90%. Currently poultry production and consumption are predicted to still increase due to a high demand for low price protein. This research is conducted to determine the clinical and pathologic anatomy due to an infection of C. jejuni on chicken digestive tract. A total of 105-day-old chickens are divided into 3 groups, each consisting of 35 individuals. Group A was the control group (not infected) group B (infected with C. jejuni ATCC 33291), and group C (infected with field isolate of C. jejuni/C1). Infection of the chicken is done 0.5 ml peroral suspension concentration of104cfu/ml. Anova Duncan is used to analyze parameters of body weight, increase weight gain, feed consumption, FCR values. Kurskall Walis method used to analyze the parameters non-parametric form of the score changes in the macroscopic and microscopic intestinal and liver. Infection of C. jejuni changes the pathology anatomy of intestine i.e. hyperemia, enteritis and enteritis catharalis haemorrhagic. A microscopic change of intestinal is oedema,haemorrhage and infiltration of inflammatory cell. Changes of pathology anatomy liver are colour smeary, congesti, pale, fragile and necrotic. Microscopic changes of liver are degeneration and focal hepatic necrosis. Group observation infected by C. jejuni caused chicken cannot reach maximum body weight and can be detrimental to farmers because of increase of the value of the feed conversion ratio (FCR).

Detection of Ampicillin Resistance Encoding Gene of Escherichia coli from Chickens in Bandung and Purwakarta Kuntum Khoirani; Agustin Indrawati; Surachmi Setiyaningsih
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 3 No. 1, JANUARY 2019
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v3i1.6134

The purpose of this study was to test the resistance and to detect antibiotic resistence encoding gene in E. coli bacteria from chickens in Bandung and Purwakarta livestock. 18 E. coli isolates were tested for antibiotics resistance using the disk diffusion method. Isolates that were categorized as resistant and intermediate to antibiotics, then polymerase chain reaction was utilized to detect the resistent coding gene. The test results showed that all E. coli isolates from chickens in Bandung and Purwakarta were resistant to ampicillin (100%). E. coli isolates were still sensitive to chloramphenicol (11.1%) and gentamicin (22.2%). The gene encoding for ampC resistance from the test were in the amount of 77.7%. Sensitivity test results and detection of resistance coding gene showed that almost all isolates were resistant to ampicillin antibiotics and E. coli isolates were still sensitive to chlorampenicol and gentamicin.

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