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All Journal Aceh Journal of Animal Science Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Jurnal Biologi Tropis JITIPARI (Jurnal Ilmiah Teknologi dan Industri Pangan UNISRI) Buletin Peternakan Tropis (Bulletin of Tropical Animal Science) Food Scientia: Journal of Food Science and Technology Jurnal Kolaboratif Sains Jurnal Pepadu Jurnal Ilmiah Pengabdian dan Inovasi JAS (Journal of Animal Science)
Ine Karni
Student of Vaccinology and Imunothrepeutics, Magister Program, Faculty of Veterinary Medicine, Universitas Airlangga, Indonesia
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Molecular characterization of Pasteurella multocida pfhaB1 gene fragment from buffalo and cattle isolates from Nusa Tenggara Timur Indonesia Ine Karni; Didik Handijatno; Lucia Tri Suwanti; Kusnoto Kusnoto; Jola Rahmahani; Wiwiek Tyasningsih
Aceh Journal of Animal Science Vol 4, No 2: December 2019
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/ajas.4.2.13581

Abstract

Almost all regions in Nusa Tenggara Timur East Nusa Tenggara (NTT) Province Indonesia are endemic areas of Haemorragic Septicaemia (HS), which is caused by Pasteurella multocida  (P. multocida ) Serotypes B: 2. The fragment  pfhaB1gene is one of the virulence factors of P. multocida.The objective of this study was to determaine the phylogenetic, homology of P. multocidapfhaB1gene fragment of isolatedfrom Buffalo and Cattle in NTT. The P. multocida isolateswere re-cultured and further microscopic examined the biochemical tests, PCR, sequencing, homology, and phylogenetic relatedness test. P. multocida was observed as gram negative, coccobacilus, no growth on MacConkey Agar, does not produce H2S and gas, nonmotile and indole positive, does not produce urease enzymes, does not use citrate as a carbon source, does not ferment maltose and lactose but it does ferment glucose, sucrose and mannitol. ThepfhaB1gene fragmentfrom buffalo and cattle NTT isolates and also Katha strain vaccine showed DNA band 506 bp. P.multocida isolates from buffalo and cattle in NTT have 91% - 99% score homology with the comparative isolate. The isolate P. multocida from buffalo and cattle in NTT are in one cluster and their phylogenetic relatedness is close to isolates from Iran and India. It is concluded that the  pfhaB1gene fragmentof P. multocida from buffalo and cattle isolates have phylogenetic relatedness close and homolog with the other comparative isolates.    Keywords: Haemorrhagic Septicaemia; Nusa Tenggara Timur; Pasteurella multocida;  pfhaB1gene
Effect of Inoculation Time for Indigenous Probiotic Bacteria Lactobacillus plantarum DAD-13 on the Physiochemical and Organoleptic Properties of Yoghurt Drink: Pengaruh Waktu Inokulasi Bakteri Probiotik Indigenous Lactobacillus plantarum DAD-13 terhadap Sifat Fisikokimia dan Organoleptik Yoghurt Drink Komalasari, Husnita; Karni, Ine; Heldiyanti, Rina; Arianto, Ahmad Rudi; Rahayu, Endang Sutriswati
JITIPARI (Jurnal Ilmiah Teknologi dan Industri Pangan UNISRI) Vol. 9 No. 1 (2024): JITIPARI
Publisher : Universitas Slamet Riyadi Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33061/jitipari.v9i1.10121

Abstract

Yoghurt drink is a product obtained from fermentation of milk by certain lactic acid bacteria. The aim of this research was to determine how the inoculation time of the indigenous probiotic bacteria Lactobacillus plantarum Dad-13 influences the physicochemical and organoleptic properties of functional plain yoghurt probiotic drink products. This research used an experimental method with a completely randomized design with a single factor, 4 treatments and 3 replications. Reaserch data were analyzed using diversity analysis and Duncan's multiple distance test at a significance level of 5% using SPSS and Minitab 17 software. The results showed that the use of probiotic bacteria significantly affected on viscosity, color L*, color a*, color b*,  °HUE, pH, total dissolved solid, and fat content, but did not significantly influenced on protein content. The use of probiotics as a starter in yoghurt drinks increased viscosity, color brightness, pH and fat content, and have a lower total dissolved solid. The best treatment in this study was joint-inoculation who had viscosity 290,67 mPa.s; color L* 89,46; color a* 2,92; color b* 1,61; °HUE 29,47; pH 5,00; total dissolved solid 16,67 °brix; fat content 2,34 %; and protein content 4,12%. For the scoring test, this treatment has a non-sour taste; the aroma is slightly sour; Slightly thick texture and homogeneous consistency. Meanwhile, the hedonic test has a neutral taste, aroma and texture as well as a color, appearance and consistency were quite liked by the panelists.
Analisis Struktur Genetik dan Filogenetik Bakteri Lactobacillus Plantarum yang Diisiolasi dari Produk Pangan Karni, Ine; Nalurita, Indah; Gemma Pravitri, Kartika; Naufali, Muhammad Nizhar; Meikapasa, Ni Wayan Putu
Food Scientia : Journal of Food Science and Technology Vol 4 No 1 (2024): January - June
Publisher : LPPM Universitas Terbuka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33830/fsj.v4i1.6488.2024

Abstract

Lactobacillus plantarum (L. plantarum) is a type of Lactic Acid Bacteria (LAB) that can convert glucose into lactic acid. L. plantarum can mostly be found in milk, meat, fermented vegetables, and the human digestive tract. The L. plantarum bacteria has several strains, each of which has a good function in food fermentation and health. To understand the historical development of an organism's evolution, it is necessary to use phylogeny as the basis for systematic science in biological evolution. The aim of this research was to determine the genetic structure and level of relationship between L. plantarum bacteria isolated from food products. The data used in this research is secondary data taken from GenBank in the form of nucleotide sequences from the bacterium L. plantarum which were then analyzed using clustal W in the Bioedit application, then genetic and phylogenetic structure analysis was carried out using the MEGA 7 application. The results showed that the probability of substitution was the highest. detected in base G, followed by bases A, T/U, and C. The L. plantarum strain that has the highest level of relationship is the strain with GenBank codes AY424355.1 and AJ640082.1 with a bootsrep value of 99. The resulting phylogenetic tree topology is monophyletic, which means that all members in the group come from a common ancestor who passed on genetic, morphological and biochemical characteristics to his descendants.
Pengaruh Waktu Penyimpanan Dan Metode Pasteurisasi Terhadap Sifat Fisikokimia Susu Kambing PE Di Kabupaten Lombok Barat: The Effect Of Storage Time And Pasteurization Method On The Physicochemical Properties Of PE Goat Milk In West Lombok District Karni, Ine; Husnita Komalasari; Kartika Gemma Pravitri; Muhammad Nizhar Naufali; Aminurrahman; Fuad Sauqi Isnain
Jurnal Kolaboratif Sains Vol. 7 No. 12: Desember 2024
Publisher : Universitas Muhammadiyah Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56338/jks.v7i12.6376

Abstract

Susu kambing merupakan hasil utama dari kambing PE bermanfaat bagi tubuh, seperti proses metabolisme tubuh karena mengandung berbagai nutrisi. Tujuan dari penelitian ini yaitu untuk mengetahui pengaruh waktu penyimpanan dan metode pasteurisasi terhadap karateristik fisikokimia susu kambing peranakan etawa (PE) di kabupaten Lombok Barat. Metode yang digunakan dalam penelitian ini adalah deskriptif dan eksperimental dengan Rancangan Acak Lengkap faktorial yang terdiri dari 2 faktor yaitu pasteurisasi dan lama penyimpanan. Data di analisa menggunakan SPSS dengan uji lanjut Duncan multiple range test (DMRT). Hasil penelitian menunjukkan bahwa faktor penyimpanan dan pasteurisasi menghasilkan pengaruh yang signifikan pada parameter pH dan warna susu, faktor pasteurisasi berpengaruh singnifikan pada WI. Sedangkan interaksi antara faktor penyimapan dan pasteurisasi memberikan pengaruh yang nyata pada parameter pH, warna dan TPT. Perlakuan terbaik pada penelitian ini adalah perlakuan pasteurisasi HTST dengan penyimpanan 0 hari, karena beberapa karakteristiknya sesuai dengan SNI seperti keadaan sampel berdasarkan warna (putih susu), aroma (aroma khas susu kuat), dan kenampakan (homogen). L* sebesar 87,08, nilai a* sebesar -1,84, nilai b* sebesar 14,85 serta nilai hue sebesar -82,94 dan nilai WI 80,23.
Penyuluhan Manajemen Beternak Sapi Potong Di Kelompok Ternak Tunas Maju Desa Setanggor, Kecamatan Praya Barat, Kabupaten Lombok Tengah: Counseling on Beef Cattle Management in Tunas Maju Livestock Group, Setanggor Village, West Praya Subdistrict, Central Lombok District Karni, Ine; Amalyadi, Rezki; Wandira, Ica Ayu; Aminurrahman; Nano Septian, I Gede; Al Gifari, Zaid; Aryadin Putra, Ryan; Anwar, Khairil
Jurnal Kolaboratif Sains Vol. 7 No. 12: Desember 2024
Publisher : Universitas Muhammadiyah Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56338/jks.v7i12.6596

Abstract

Penyuluhan manajemen beternak sapi potong memiliki peran penting dalam meningkatkan produktivitas dan kesejahteraan peternak. Penelitian ini bertujuan untuk mengevaluasi efektivitas penyuluhan manajemen beternak sapi potong yang dilakukan di Kelompok Ternak Tunas Maju, Desa Setanggor, Kecamatan Praya Barat, Kabupaten Lombok Tengah. Metodologi yang digunakan meliputi survei, wawancara, dan observasi langsung terhadap anggota kelompok ternak. Hasil penelitian menunjukkan bahwa penyuluhan yang diberikan berhasil meningkatkan pengetahuan dan keterampilan peternak dalam aspek manajemen pakan, kesehatan ternak, dan reproduksi. Peternak yang mengikuti penyuluhan secara aktif menunjukkan peningkatan signifikan dalam praktik beternak yang lebih efisien dan berkelanjutan. Selain itu, hasil evaluasi juga mengungkapkan bahwa penyuluhan membantu mengatasi beberapa masalah yang dihadapi peternak, seperti kekurangan pakan dan penyakit ternak. Kesimpulannya, penyuluhan manajemen beternak sapi potong di Kelompok Ternak Tunas Maju telah memberikan dampak positif terhadap peningkatan produktivitas dan kesejahteraan peternak. Rekomendasi untuk penyuluhan selanjutnya adalah peningkatan frekuensi dan variasi materi penyuluhan, serta pengembangan program pelatihan yang lebih komprehensif untuk menjawab kebutuhan spesifik peternak di wilayah tersebut.
Detection of Brucellosis in Bali Cattle (Bos Sondaicus ) in Bolo District, Bima Regency Karni, Ine; Lukman HY; Kuntum Khoirani; Burhan; Oscar Yanuariyanto
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 7, No 1, JANUARY 2023
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v7i1.21279

Abstract

This study aims to determine the incidence of Brucellosis in Bali cattle in Bolo district, Bima regency, based on serological tests (seroprevalence). The sample used in this study was cattle serum. Serum samples were obtained from 70 Bali cattle aged over one year. The detection method, namely the Rose Bengal Test, detects the incidence of Brucellosis. Samples in the form of serum are reacted with Brucella sp. antigen to see antibodies in the serum. The working principle of the Rose Bengal Test is to see whether there is agglutination or not. The results of this study will be an indicator to determine the presence or absence of Brucellosis in Bali cattle in Bolo district, Bima regency. Then the monitoring and control can be established by relevant stakeholders, especially the Livestock and Animal Health Service of Bima Regency. The data obtained from serum samples that describe the positive or negative for Brucellosis is presented descriptively by explaining each sample origin's seropositive and seronegative results. The results showed that in all samples from Bali cattle in the District of Bolo, as many as 70 samples reacted negatively to the Rose Bengal Test. This result indicates that the cattle on Sumbawa Island are not infected with Brucellosis. Keywords: Brucellosis, Reproductive Disorders, Bali Cattle, Rose Bengal Test
Sosialisasi Pembuatan Nomor Induk Berusaha (NIB) sebagai Dokumen Awal Sertifikasi Halal bagi UMKM Nalurita, Indah; Pravitri, Kartika Gemma; Karni, Ine; Putri, Destiana Adinda; Awaliyah, Mia
Jurnal Ilmiah Pengabdian dan Inovasi Vol. 2 No. 2 (2023): Jurnal Ilmiah Pengabdian dan Inovasi (Desember)
Publisher : Insan Kreasi Media

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.57248/jilpi.v2i2.310

Abstract

The perception of Small and Medium Enterprise (UMKM) regarding the complexity of processing the files required for Business Identification Numbers (NIB) and halal certification is a factor in the low enthusiasm of UMKM members in processing these permits. The percentage of UMKM members in Narmada who have NIB permits is 40%, while the percentage of UMKM members who have halal certificates is only 2%. The method of service activities using socialization and discussion is considered quite appropriate for exploring the problems experienced by UMKM, so that they can obtain effective solutions. The socialization activity for making NIB as the initial document for halal certification for UMKM in Narmada is important to carry out as an effort to help simplify the processing of product licensing for UMKM.
Physical Quality and Organoleptic Quality of Mutton Meatballs with The Addition of Cassava Flour at Different Levels: Kualitas Fisik Dan Mutu Organoleptik Bakso Daging Kambing Dengan Penambahan Tepung Singkong Pada Level Yang Berbeda Amalyadi, Rezki; Karni, Ine; Gifari, Zaid Al; Septian, I Gede Nano; Aminurrahman, Aminurrahman
Buletin Peternakan Tropis Vol. 6 No. 1 (2025)
Publisher : BPFP Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/bpt.6.1.15-24

Abstract

This study aims to determine the effect of the addition of cassava flour in various levels on the physical and organoleptic quality of meatballs made from mutton. The physical and organoleptic quality tests included measurements of pH, cooking shrinkage, yield, aroma, taste, colour, and texture. The research design applied was a completely randomized design (CRD) with four treatments and five repetitions. For data analysis, ANOVA was used on physical parameters and organoleptic test with Kruskal Wallis method. The findings of the study showed that the highest pH, cooking shrinkage, and yield were achieved in P3, which was with the addition of cassava flour at the level of 20%. Aroma, flavour, and texture did not show a significant effect on the variation of the level of cassava flour addition. However, colour has a significant effect related to the level of cassava flour addition of 20%. It can be concluded that the addition of cassava flour at the level of 20% can affect the colour, pH, cooking shrinkage and yield of mutton meatballs.
Integration Of Genetic And Nutritional Factors In The Production And Quality Of Dairy Goat Milk: A Review: Integrasi Faktor Genetik Dan Nutrisi Dalam Produksi Dan Kualitas Susu Kambing Perah : Review Karni, Ine; Amalyadi, Rezki; Aminurrahman, Aminurrahman; Wandira, Ica Ayu; Gifari, Zaid Al; Septian, I Gede Nano; Anwar, Khairil
Buletin Peternakan Tropis Vol. 6 No. 1 (2025)
Publisher : BPFP Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/bpt.6.1.57-71

Abstract

The need for animal protein in Indonesia continues to increase, driving the popularity of goat milk as an alternative to cow's milk that is easier to digest and has health benefits. This study aims to review genetic and nutritional factors that affect the production and quality of dairy goat milk. The method used is a literature review of relevant scientific sources. Data analysis was carried out qualitatively. The results of the study indicate that genetic and nutritional factors play a major role in goat milk production. Imported goats such as Alpine (2,633 liters/lactation) and Saanen (2,621 liters/lactation) are more productive than local breeds such as Sapera (264.6 liters/lactation) and Peranakan Etawa (154.3 liters/lactation), supported by strict genetic selection and optimal maintenance management. Milk composition also varies, with Senduro goats having the highest total solids (14.30%), protein (3.74%), and fat (6.40%) compared to Alpine. Nutrition plays an important role in supporting milk production, with high protein sources such as lamtoro (32.0%) and turi (31.29%), as well as fat from turi (7.57%) and indigofera (6.15%) which increase milk fat content. Genetic and nutritional factors affect the production and quality of goat milk, with imported goats being more productive and Senduro goats being superior in milk composition. Proper feed and good management improve milk quality and livestock health. Understanding these genetic and nutritional factors is expected to be the basis for efforts to increase the productivity and quality of dairy goat milk in Indonesia
PENYULUHAN MANAJEMEN PEMELIHARAAN TERNAK SAPI KELOMPOK TERNAK IYE GATI DI DESA SUKADANA, KECAMATAN PUJUT, KABUPATEN LOMBOK TENGAH Aminurrahman, Aminurrahman; Depamede, Sulaiman Ngongu; Suhardiani, Roro Agustien; Purnamasari, Dwi Kusuma; Noersidiq, Azhary; Amalyadi, Rezki; Karni, Ine; Septian, I Gede Nano; Musanip, Musanip; Fahrullah, Fahrullah; Muhsinin, Muhammad; Maslami, Vebera; Gifari, Zaid Al
Jurnal Pepadu Vol 6 No 1 (2025): Jurnal Pepadu
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/pepadu.v6i1.6720

Abstract

Penyuluhan manajemen ternak sapi diberikan kepada kelompok ternak Iye Gati diDesa Sukadana Kecamatan Pujut Lombok Tengah untuk meningkatkan keterampilan dalampemeliharaan, perkandangan, kesehatan, dan reproduksi sapi potong. Keberhasilanpeternakan bergantung pada pembibitan, pakan, dan manajemen pemeliharaan, denganfaktor non-genetik berkontribusi besar terhadap efisiensi reproduksi. Pengabdian inibertujuan untuk mengevaluasi efektivitas penyuluhan manajemen pemeliharaan ternak sapiyang dilakukan di Kelompok Ternak Iye Gati di Desa Sukadana Kecamatan PujutKabupaten Lombok Tengah. Kurangnya pengetahuan peternak dalam pemeliharaanmenjadi tantangan utama. Penyuluhan dan pelatihan berperan penting dalam meningkatkanwawasan peternak serta mendukung ketahanan pangan dan pertanian berkelanjutan (SDGspoin 2). Kegiatan pengabdian masyarakat ini dilakukan melalui survei, wawancara,observasi, pemaparan materi, dan diskusi untuk meningkatkan produktivitas peternakan diDesa Sukadana. Hasil penelitian menunjukkan bahwa penyuluhan yang diberikan berhasilmeningkatkan pengetahuan dan keterampilan peternak dalam aspek manajemenpemeliharaan. Peternak yang mengikuti penyuluhan secara aktif menunjukkan peningkatansignifikan dalam praktik beternak yang lebih efisien dan berkelanjutan. Selain itu, hasilevaluasi juga mengungkapkan bahwa penyuluhan membantu mengatasi beberapa masalahyang dihadapi peternak, seperti kekurangan pakan dan penyakit ternak. Kesimpulannya,penyuluhan manajemen pemeliharaan ternak sapi di Kelompok Ternak Desa Sukadana telahmemberikan dampak positif terhadap peningkatan produktivitas dan kesejahteraanpeternak. Rekomendasi untuk penyuluhan selanjutnya adalah peningkatan frekuensi danvariasi materi penyuluhan, serta pengembangan program pelatihan yang lebih komprehensifuntuk menjawab kebutuhan spesifik peternak di wilayah tersebut.
Co-Authors Aminurrahman Arianto, Ahmad Rudi Ashari, M. Azhary Noersidiq Burhan Didik Handijatno Dohi, Muhammad Dwi Kusuma Purnamasari, Dwi Kusuma Endang Sutriswati Rahayu Fahrullah Fahrullah Fahrullah, Fahrullah Fuad Sauqi Isnain Hasma Hasma Heldiyanti, Rina Husnita Komalasari Indah Nalurita Kartika Gemma Pravitri Khairil Anwar Kuntum Khoirani Kusnoto Kusnoto Lucia Tri Suwanti, Lucia Tri Lukman HY Made Sriasih, Made Muhamad Ali Muhammad Nizhar Naufali Muhsinin, Muhammad Musanip, Musanip Ni Ketut Dewi Haryani, Ni Ketut Dewi Noersidiq, Azhari Oscar Yanuariyanto Poerwoto, Happy Putra, Ryan Aryadin Putri, Destiana Adinda PUTU MEIKAPASA, NI WAYAN Rahmahani, Jola Rezki Amalyadi Rina Andriati, Rina Rosyidi, Muhammad Subhan Bahruddin Septian, I Gede Nano Suhardiani, Roro Agustien Suhardiani, Rr. Agustien Sukarne Sukarne Sulaiman Ngongu Depamede Suryadi, Muh. Aodil Fitriyan Fadjar Taqiuddin, Moh Tyasningsih, Wiwiek Unsunnidhal, Lalu Vebera Maslami Wandira, Ica Ayu Zaid Al Gifari