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All Journal Jurnal Sain Peternakan Indonesia Journal of the Indonesian Tropical Animal Agriculture
E. Kurnianto
Department of Animal Science, Faculty of Animal and Agricultural Sciences, Universitas Diponegoro
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Veterinary

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Evaluation of Native Rooster Based on Breeding Value of trait of Macroscopic and Microscopic Semen Quality Sutiyono Sutiyono; E. Kurnianto; Sutopo Sutopo; D. K. Ariyanto; D. Samsudewa
Jurnal Sain Peternakan Indonesia Vol 16, No 3 (2021)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jspi.id.16.3.228-232

Abstract

The aim of this research is to a determined the breeding value of native roosters based on macroscopic and microscopic semen. Ten native roosters were used in this research. Complete individual cage, semen collection tools and substance, macroscopic and microscopic tools, and substance were used in this research. The native rooster was rested for 2 weeks. Massage techniques were used for semen collection. Parameters observed were volume, pH, color, viscosity, smell, sperm concentration, and sperm livability. The semen collection was repeated 5 times. One-way analysis of variances was used for data analysis with the tools SPSS 16. The breeding value was estimated based on primary traits of semen and calculated for each rooster to make a rank. The results showed that significant parameters (P<0,05) are semen volume and sperm concentration, while the non-significant parameters (P?0,05) are pH semen and sperm livability. Semen color is yellowish-white, semen viscosity is medium-viscous and smell is spermin. The results of breeding value rank are rooster numbers 6, 7, 5, 3, 2, 4, 8, 9, 10, and 1. The conclusion is the breeding value of Native roosters is varied. The recommendation is Native chicken breeding need to apply selection used breeding value based on semen quality.
Follicle-stimulating hormone receptor gene exploration as possible markers for prolific trait of local goat in Indonesia E. T. Setiatin; D. A. Lestari; D. W. Harjanti; A. Setiaji; E. Kurnianto
Journal of the Indonesian Tropical Animal Agriculture Vol 48, No 1 (2023): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.48.1.28-36

Abstract

The objective of this study was to discover and identify the effect of SNP of follicle-stimulating hormone receptor (FSHR) gen on prolific traits in Kejobong (KJ) and Etawah grade (EG) doe. A total of 15 blood samples were taken from 11 KJ and 4 EG with various parity and type of birth. The FSHR gene was amplified from the DNA template by Polymerase Chain Reaction (PCR); the PCR products were then sequenced to determine Single Nucleotide Polymorphisms (SNP). Results showed that 3 SNPs were identified, and those SNPs altered amino acid sequence formed 6 haplotypes and divided the doe based on the type of birth. In conclusion, SNPs identified in this study are associated with a prolific trait that can be used as a genetic marker and haplotype 3, 4, and 5 showed the highest prolific on KJ and EG doe.
Polymorphism of SNP g.8398A>G at prolactin gene and its effect on Indonesian Holstein dairy cow’s milk performance and reproductive traits Y. W. Setyorini; S. Sutopo; E. Kurnianto; S. Sutiyono
Journal of the Indonesian Tropical Animal Agriculture Vol 48, No 1 (2023): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.48.1.10-19

Abstract

The objective of this study was to identify the polymorphism of Single Nucleotide Polymorphism (SNP) g.8398A>G at the prolactin gene exon 4 in Indonesian Holstein dairy cows and its effect on milk production, milk quality, and reproductive traits. A total of 140 blood samples were investigated to detect polymorphism by PCR-RFLP method using the RsaI restriction enzyme. Sequencing was per-formed for confirmation of SNP mutation points. The phenotype data collected were milk production, milk quality, and reproductive traits. Data were analyzed using a t-test. The results showed that poly-morphism of SNP g.8398A>G at prolactin gene exon 4 was found in the study populations with AG and GG genotypes. Cows with the GG genotype indicated higher total milk yield and milk protein (P<0.05) than the AG genotype. Polymorphism was not associated with specific gravity, milk fat, milk lactose, total solid non-fat, and reproductive traits. SNP g.8398A>G at prolactin gene exon 4 showed potential as a genetic marker for selecting superior milk production traits.
Co-Authors A. Setiaji D. A. Lestari D. K. Ariyanto D. Samsudewa D. W. Harjanti E. T. Setiatin S. Sutiyono S. Sutopo Sutiyono Sutiyono Sutopo Sutopo Y. W. Setyorini