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All Journal Journal of the Indonesian Tropical Animal Agriculture
A. Setiaji
Department of Animal Science, Faculty of Animal and Agricultural Sciences, Universitas Diponegoro
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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Seasonal effect on semen quality of Limousine and Simmental bulls F. R. Hapsari; D. Samsudewa; E. T. Setiatin; Y. S. Ondho; A. Setiaji; T. Harsi; I. Z. Irfan; O. Syamsono; E. Sukmawati
Journal of the Indonesian Tropical Animal Agriculture Vol 47, No 4 (2022): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.47.4.340-345

Abstract

This study aimed to determine the effect of breed and season on the semen quality of AI bulls. The material used was n= 78,648 records of ejaculated semen from 85 Limousine and 88 Simmental bulls collected between period 2010 and 2020. Semen qualities evaluated in the study were: ejaculate vol-ume (Vol), pH, individual sperm motility (ISM), sperm concentration (SC), and post-thawing motility (PTM). The data was analyzed by using a MIXED model with breeds and seasons as fixed effects and animals as random effects. The seasons were significant (p<0,05) on Vol, ISM, SC, and PTM, then the interaction between season and breed was affected on Vol, SC, and PTM. Simmental bulls generally showed better semen qualities than Limousine bulls. Further, the same result showed significant differ-ences in each season. The Vol, ISM, SC, and PTM of Simmental were 6.48 ± 1.26, 64.22 ± 1.16, 1,108 ± 0.46, and 42.19 ± 0.38, respectively. The increase of Vol and SC in the humid season has not propor-tionally affected ISM.
Follicle-stimulating hormone receptor gene exploration as possible markers for prolific trait of local goat in Indonesia E. T. Setiatin; D. A. Lestari; D. W. Harjanti; A. Setiaji; E. Kurnianto
Journal of the Indonesian Tropical Animal Agriculture Vol 48, No 1 (2023): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.48.1.28-36

Abstract

The objective of this study was to discover and identify the effect of SNP of follicle-stimulating hormone receptor (FSHR) gen on prolific traits in Kejobong (KJ) and Etawah grade (EG) doe. A total of 15 blood samples were taken from 11 KJ and 4 EG with various parity and type of birth. The FSHR gene was amplified from the DNA template by Polymerase Chain Reaction (PCR); the PCR products were then sequenced to determine Single Nucleotide Polymorphisms (SNP). Results showed that 3 SNPs were identified, and those SNPs altered amino acid sequence formed 6 haplotypes and divided the doe based on the type of birth. In conclusion, SNPs identified in this study are associated with a prolific trait that can be used as a genetic marker and haplotype 3, 4, and 5 showed the highest prolific on KJ and EG doe.
Effect of faecal microbiota transplantation on growth, haematological and intestinal indices of Kedu chickens M. R. Yousaf; A. N. M. Ramli; D. A. Lestari; S. Sutaryo; I. Agusetyaningsih; T. Ayasan; N. S. Pandupuspitasari; A. Setiaji; S. Sugiharto
Journal of the Indonesian Tropical Animal Agriculture Vol 51, No 3 (2026): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.51.3.162-170

Abstract

The study examined the effect of lyophilized fecal microbiota transplantation (FMT) from healthy chicken donors on growth performance, haematological indices and intestinal morphology of Kedu chickens during the starter phase. A number of 120 Kedu chickens (0-6 weeks old) were randomly as-signed to T0 (orally inoculated with 1 mL PBS), T1 (inoculated with 0.5 mL FMT) and T2 (1.0 mL FMT). Body weight and feed intake were weekly recorded, while blood and intestinal segments were collected at week 6. Results showed that live body weight and body weight gain were highest (p<0.001) in T1, and the lowest in T0 group. Feed conversion ratio (FCR) was better (p=0.001) in T1 and T2 compared to T0 group. Feed intake was lower (p=0.014) in T2 than in T0 and T1 groups. The FMT administration tended to increase erythrocytes (p=0.054) and haemoglobin levels (p=0.056) of Kedu chickens. The relative weight of duodenum, jejunum, ileum, caecum and colon were lower (p<0.05) in T1 and T2 compared to those in T0 group. The FMT administration had no effect (p>0.05) on duodenal and jejunal villi height, crypt depth and the ratio of villi height to crypt depth of Kedu chickens. In conclusion, daily low-dose lyophilized FMT was associated with improved growth perfor-mance and feed efficiency in starter Kedu chickens, while haematological and intestinal morphological responses were limited.
Gomperzt non-linear model for predicting growth performance of commercial broiler chickens A. Setiaji; D. A. Lestari; B. Ma&#039;rifah; L. Krismiyanto; I. Agusetyaningsih; S. Sugiharto
Journal of the Indonesian Tropical Animal Agriculture Vol 48, No 2 (2023): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.48.2.143-149

Abstract

An experiment was conducted to estimate growth parameters for commercial broiler chickens in Indonesia. The data was collected from July 2021 to June 2022. A total of 1,570 samples consisting of four strains of broiler chickens were collected from 74 houses. The samples were daily weighed from 0 to 7 days of age, and they were weekly weighed from 1 to 5 weeks of age. A nonlinear Gompertz growth model was fitted to the observed body weights (BW). The results for five growth parameters were as follows: the asymptotic value (A) of the mature live weight ranged from 3.733 to 5.044 kg; the turning point of growth (B) ranged from 4.499 to 4.561; the value growth rate constant (K) ranged from 0.049 to 0.059 kg/week; Inflection points in ranged from 25.292 – 30.970 days, and 1.373 – 1.855 kg for inflection age (IA) and inflection weight (IW), respectively. The model was an excellent fit for the growth data in the commercial broiler with a low Akaike information criterion (AIC), and high coeffi-cient determination (R2).
Association analysis of the transforming growth factor factor-β2 gene with growth performance in Kedu chickens M. Akramullah; E. Kurnianto; D. A. Lestari; E. T. Setiatin; D. Y. Palupi; Y. N. Anjani; G. P. Dewi; A. Setiaji
Journal of the Indonesian Tropical Animal Agriculture Vol 51, No 1 (2026): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.51.1.72-81

Abstract

The Transforming Growth Factor-β2 (TGF-β2) gene is one of the cytokine genes that plays an important role in regulating growth processes and disease resistance. This study aimed to analyze the association between the TGF-β2 gene and growth performance in Kedu chickens. A total of 130 Kedu chickens, consisting of 72 males and 58 females, were observed for 10 weeks. Growth parameters measured included body weight, feed intake, body weight gain, and feed conversion ratio. Identifica-tion of the single nucleotide polymorphism (SNP) in the TGF-β2 gene was performed using the PCR-RFLP method. The association analysis between the g.640 T>C SNP polymorphism of the TGF-β2 gene and growth performance was conducted using analysis of variance under a General Linear Model (GLM). The results showed that the g.640 T>C SNP of the TGF-β2 gene was polymorphic and not in Hardy–Weinberg equilibrium. The TT genotype exhibited better growth performance (body weight gain, feed intake, and feed conversion ratio) than the CC genotype, particularly at 10 weeks of age in both female and unsexed chickens.
DNA variants and population structure of Magelang ducks across generation A. Febriana; E. Kurnianto; S. Sutopo; D. A. Lestari; A. Setiaji; S. Sugiharto
Journal of the Indonesian Tropical Animal Agriculture Vol 49, No 1 (2024): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.49.1.67-77

Abstract

Magelang ducks (MD) are Indonesian local ducks and are known as dual-purpose type ducks which have high egg production, duck day production (DDP), and body weight size. Nowadays, the MD is selected to establish the pure line to advance the egg production trait in the Breeding and Rearing Center of Non-Ruminant Animals Banyubiru, Central Java. The mitochondrial DNA (mtDNA) Dloop region, is highly polymorphic and could be used to analyze the population genetics. The present research aims to examine the impacts of continuous selection on population structure and genetic mutations on MD across two generations using the mtDNA D-loop region. Thirty-six blood samples from the second (G2) and third (G3) generations were examined using the sequencing method. The MEGA X and DnaSP software were applied to calculate the genetic diversity, genetic distance, and to generate a phylogenetic tree. The number of haplotypes (H), haplotype diversity (Hd), and Tajima's D are 26, 0.9746, and -1.46, respectively. The Fst value of MD is 0.156. The genetic distance among populations ranges from 0.0000 – 2.097. The UPGMA analysis constructs one clade in a phylogenetic tree between MD, Indonesian local Ducks, Indian Ducks, Vietnamese Ducks, and Chinese Ducks. The study found that the genetic variation and population structure did not significantly change between the second and third generations.
Co-Authors A. Febriana A. N. M. Ramli B. Ma&#039;rifah D. A. Lestari D. Samsudewa D. W. Harjanti D. Y. Palupi E. Kurnianto E. Sukmawati E. T. Setiatin F. R. Hapsari G. P. Dewi I. Agusetyaningsih I. Z. Irfan L. Krismiyanto M. Akramullah M. R. Yousaf NURULIARIZKI SHINTA PANDUPUSPITASARI O. Syamsono S. Sugiharto S. Sutaryo S. Sutopo T. Ayasan T. Harsi Y. N. Anjani Y. S. Ondho