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E. T. Setiatin
Department of Animal Science, Faculty of Animal and Agricultural Sciences, Universitas Diponegoro
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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Seasonal effect on semen quality of Limousine and Simmental bulls F. R. Hapsari; D. Samsudewa; E. T. Setiatin; Y. S. Ondho; A. Setiaji; T. Harsi; I. Z. Irfan; O. Syamsono; E. Sukmawati
Journal of the Indonesian Tropical Animal Agriculture Vol 47, No 4 (2022): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.47.4.340-345

Abstract

This study aimed to determine the effect of breed and season on the semen quality of AI bulls. The material used was n= 78,648 records of ejaculated semen from 85 Limousine and 88 Simmental bulls collected between period 2010 and 2020. Semen qualities evaluated in the study were: ejaculate vol-ume (Vol), pH, individual sperm motility (ISM), sperm concentration (SC), and post-thawing motility (PTM). The data was analyzed by using a MIXED model with breeds and seasons as fixed effects and animals as random effects. The seasons were significant (p<0,05) on Vol, ISM, SC, and PTM, then the interaction between season and breed was affected on Vol, SC, and PTM. Simmental bulls generally showed better semen qualities than Limousine bulls. Further, the same result showed significant differ-ences in each season. The Vol, ISM, SC, and PTM of Simmental were 6.48 ± 1.26, 64.22 ± 1.16, 1,108 ± 0.46, and 42.19 ± 0.38, respectively. The increase of Vol and SC in the humid season has not propor-tionally affected ISM.
Follicle-stimulating hormone receptor gene exploration as possible markers for prolific trait of local goat in Indonesia E. T. Setiatin; D. A. Lestari; D. W. Harjanti; A. Setiaji; E. Kurnianto
Journal of the Indonesian Tropical Animal Agriculture Vol 48, No 1 (2023): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.48.1.28-36

Abstract

The objective of this study was to discover and identify the effect of SNP of follicle-stimulating hormone receptor (FSHR) gen on prolific traits in Kejobong (KJ) and Etawah grade (EG) doe. A total of 15 blood samples were taken from 11 KJ and 4 EG with various parity and type of birth. The FSHR gene was amplified from the DNA template by Polymerase Chain Reaction (PCR); the PCR products were then sequenced to determine Single Nucleotide Polymorphisms (SNP). Results showed that 3 SNPs were identified, and those SNPs altered amino acid sequence formed 6 haplotypes and divided the doe based on the type of birth. In conclusion, SNPs identified in this study are associated with a prolific trait that can be used as a genetic marker and haplotype 3, 4, and 5 showed the highest prolific on KJ and EG doe.
Association analysis of the transforming growth factor factor-β2 gene with growth performance in Kedu chickens M. Akramullah; E. Kurnianto; D. A. Lestari; E. T. Setiatin; D. Y. Palupi; Y. N. Anjani; G. P. Dewi; A. Setiaji
Journal of the Indonesian Tropical Animal Agriculture Vol 51, No 1 (2026): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.51.1.72-81

Abstract

The Transforming Growth Factor-β2 (TGF-β2) gene is one of the cytokine genes that plays an important role in regulating growth processes and disease resistance. This study aimed to analyze the association between the TGF-β2 gene and growth performance in Kedu chickens. A total of 130 Kedu chickens, consisting of 72 males and 58 females, were observed for 10 weeks. Growth parameters measured included body weight, feed intake, body weight gain, and feed conversion ratio. Identifica-tion of the single nucleotide polymorphism (SNP) in the TGF-β2 gene was performed using the PCR-RFLP method. The association analysis between the g.640 T>C SNP polymorphism of the TGF-β2 gene and growth performance was conducted using analysis of variance under a General Linear Model (GLM). The results showed that the g.640 T>C SNP of the TGF-β2 gene was polymorphic and not in Hardy–Weinberg equilibrium. The TT genotype exhibited better growth performance (body weight gain, feed intake, and feed conversion ratio) than the CC genotype, particularly at 10 weeks of age in both female and unsexed chickens.
Co-Authors A. Setiaji D. A. Lestari D. Samsudewa D. W. Harjanti D. Y. Palupi E. Kurnianto E. Sukmawati F. R. Hapsari G. P. Dewi I. Z. Irfan M. Akramullah O. Syamsono T. Harsi Y. N. Anjani Y. S. Ondho