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Active compounds isolated from red betel (Piper crocatum Ruiz & Pav) leaves active against Streptococcus mutans through its inhibition effect on glucosyltransferase activity Mustofa, Rima Erviana Setyo Purwono
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 43, No 02 (2011)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (194.83 KB)

Abstract

Dental caries is a major problem of dental health in Indonesia. Streptococcus mutans is a bacteria that can causecaries. In this recent time, the agents used to eliminate the caries is less effective due to S. mutans resistance tothose agents. Moreover, the agents may produce side effects after 10 years of consumption.Therefore, effort tofind alternative agents against dental caries is needed. Red betel (Piper crocatum Ruiz & Pav) is a promising naturalmaterial to treat dental caries. The P. crocatum Ruiz & Pav leaves contain secondary metabolites such as essentialoil, flavonoid, alkaloid, and phenolic compounds that active against S. mutans involved in caries formation. Thisstudy aimed to evaluate the activity of secondary metabolites isolated from red betel leaves against S. mutans andits inhibition effect on the glucosyltransferase (GTF) activity. The growth inhibition test of S. mutans was carriedout with liquid dilution test and diffusion test. Cultures of S. mutans were treated with essential oil, alkaloid,flavonoid, phenolic compounds isolated from P. crocatum Ruiz & Pav in various concentration and incubated at 37OCin anaerob condition. Povidone iodine was used as positive control and aquadest as negative control. The inhibitiontest of GTF activity was performed by measuring the levels of formed fructose that was analyzed by Somogyinelsonmethod. The data was analyzed using one-way ANOVA with a 95% confidence interval.The results showedthat essential oil and alkaloid had lowest MIC (Minimal Inhibitory Concentration) compared with the other activecompounds (5000 μg/mL) but higher than povione iodine as positive control (2500 μg/mL). The essential oil exhibitedsimilar inhibition zone diameter compared with povidone iodine, either at low or high concentration (p>0.05). Theessential oil had similar inhibition activity to the glucosyltransferase compared with povidone iodine (p>0.05). Inconclusion, the essential oil of red betel leaves had the strongest activity against the S. mutans growth through itsinhibition effect on GTF activity.Key words : red betel (P. crocatum Ruiz & Pav) – antibacterial - S. mutans – essential oil - glucosyltransferase
Active compounds isolated from red betel (Piper crocatum Ruiz & Pav) leaves active against Streptococcus mutans through its inhibition effect on glucosyltransferase activity Rima Erviana Setyo Purwono Mustofa
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 43, No 02 (2011)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (194.83 KB)

Abstract

Dental caries is a major problem of dental health in Indonesia. Streptococcus mutans is a bacteria that can causecaries. In this recent time, the agents used to eliminate the caries is less effective due to S. mutans resistance tothose agents. Moreover, the agents may produce side effects after 10 years of consumption.Therefore, effort tofind alternative agents against dental caries is needed. Red betel (Piper crocatum Ruiz & Pav) is a promising naturalmaterial to treat dental caries. The P. crocatum Ruiz & Pav leaves contain secondary metabolites such as essentialoil, flavonoid, alkaloid, and phenolic compounds that active against S. mutans involved in caries formation. Thisstudy aimed to evaluate the activity of secondary metabolites isolated from red betel leaves against S. mutans andits inhibition effect on the glucosyltransferase (GTF) activity. The growth inhibition test of S. mutans was carriedout with liquid dilution test and diffusion test. Cultures of S. mutans were treated with essential oil, alkaloid,flavonoid, phenolic compounds isolated from P. crocatum Ruiz & Pav in various concentration and incubated at 37OCin anaerob condition. Povidone iodine was used as positive control and aquadest as negative control. The inhibitiontest of GTF activity was performed by measuring the levels of formed fructose that was analyzed by Somogyinelsonmethod. The data was analyzed using one-way ANOVA with a 95% confidence interval.The results showedthat essential oil and alkaloid had lowest MIC (Minimal Inhibitory Concentration) compared with the other activecompounds (5000 μg/mL) but higher than povione iodine as positive control (2500 μg/mL). The essential oil exhibitedsimilar inhibition zone diameter compared with povidone iodine, either at low or high concentration (p>0.05). Theessential oil had similar inhibition activity to the glucosyltransferase compared with povidone iodine (p>0.05). Inconclusion, the essential oil of red betel leaves had the strongest activity against the S. mutans growth through itsinhibition effect on GTF activity.Key words : red betel (P. crocatum Ruiz & Pav) – antibacterial - S. mutans – essential oil - glucosyltransferase
Implementasi Supervisi Manajerial Pengawas Sekolah Pada Madrasah di Kabupaten Sukabumi Hermansyah, Ade; Jaelani, Deny Ahmad; Erviana, Rima; Afifah, Siti Nur; Khadafi, Alfian
Jurnal Pendidikan West Science Vol 1 No 09 (2023): Jurnal Pendidikan West Science
Publisher : Westscience Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58812/jpdws.v1i09.1085

Abstract

Dalam Undang-undang Sisdiknas Nomor 20 tahun 2003 dijelaskan bahwa tenaga kependidikan adalah anggota masyarakat yang mengabdikan diri dan diangkat untuk menunjang penyelenggaraan pendidikan. Termasuk didalamnya para pengawas yang berkedudukan sebagai supervisor dan fasilitator dalam upaya peningkatan mutu pendidikan. Penelitian kualitatif dipilih sebagai jenis penelitian yang digunakan dalam penelitian ini. Dengan pendekatan fenomenologi dengan paradigma konstruktivis. Pada penelitian fenomenologi metode pengumpulan data yang digunakan berfokus pada interview mendalam (in-depth interviews) dan narasi (narratives) sebagai langkah-langkah utama dalam membuat penjelasan dan penggambaran dari pengalaman yang pernah dialami dalam hidup dan data melalui metode dokumentasi (documentary methods) atau metode visual (visual methods). Dalam pengumpulan data pada penelitian ini dibuat menggunakan jenis data yang diperlukan dalam pengumpulan informasi, data yang dibutuhkan berupa data primer dan data sekunder berupa observasi, wawancara dan dokumentasi. Setelah penyajian data hal yang dilakukan selanjutnya menarik kesimpulan dari analisis data-data penelitian yang dilakukan. Pengawas sekolah pada madrasah melaksanakan supervisi manajerial di Kabupaten Sukabumi dengan baik sesuai dengan tugas dan tanggung jawab sebagaimana yang diamanahkan permen. Secara umum dari hasil temuan peneliti atas objek penelitian ini, dapat digarisbawahi, bahwa pelaksanaan supervisi manajerial berjalan dengan baik sebagaimana mestinya. Dari hal ini, pihak pengawas atau sekolah tidak boleh puas dengan pekerjaan atau komunikasi yang sudah dibina selama ini terhadap sekolah tersebut. Namun, yang perlu untuk dicermati, didiskusikan dan diperhatikan juga adalah bagaimana aktivitas supervisi manajerial tetap berlangsung dengan baik, menghasilkan outcome yang diharapkan, dan tidak merasa puas terhadap hasil yang tertinggi, baik itu dari pihak pengawas sekolah maupun warga sekolah itu sendiri.