Article Per Year (5 Year)
p-Index From 2020 - 2025
0.23
P-Index
This Author published in this journals
All Journal Jurnal Ilmu-Ilmu Pertanian Indonesia
Hendri Bustamam
University of Bengkulu
Agriculture, Biological Sciences & Forestry

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
SELEKSI MIKROBA RIZOSFER ANTAGONIS TERHADAP BAKTERI Ralstolnia solanacearum PENYEBAB PENYAKIT LAYU BAKTERI PADA TANAMAN JAHE DI LAHAN TERTINDAS Hendri Bustamam
Jurnal Ilmu-Ilmu Pertanian Indonesia Vol 8, No 1 (2006)
Publisher : Program Studi Ilmu Tanah Fakultas Pertanian UNIB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jipi.8.1.12-18

Abstract

Bacterial wilt disease caused by Ralstolnia solanacearum had decreased the ginger production. Saphrophyte and antagonistic microbe are potential to control this disease by Integrated application. This microbes could be found at supressive land for pathogens. Research due to select the potential microbes to control bacterial wilt disease on ginger. Microbe was isolated from rhizosfer soil and root of healthy crop on infected ginger cropping by Ralstolnia solanacearum in Bengkulu by plate dilution methode using Pepton Glucose Agar (PGA) and Ginger-Potato Dextrose Agar (GPDA). Antagonistic was tested by Double Culture Technique on PGA and GPDA. Selected saphrophyte and antagonistic isolat was cultured on organic medium and tested to ginger crop that grown on infected soil. Disease development was observated until 5 months-age crop. The result of this experiment had isolated 4 fungi isolates and 4 bacteria isolates that potential as biocontrol agent to Ralstolnia solanacearum. Tested on Var. Badak ginger cropping indicated that all isolates had reduced 64-84% of diseases. Five Isolates of Pennicilium digitatum, Trichoderma harzianum, T. viride, Achromobacter sp., and Pseudomonas fluorescence was protected the crop to zero diseases; while three isolates of Trichoderma koningii, Bacillus sp., and Pseudomonas putida was protected the crop to 4% infected. Amundment of 12 isolates cultured on organic  matter can improve the growth of false stem 11.11 – 96.97%; leave number 8.29 - 156%; and plant height 27.68 – 93.75%.
PENYAKIT TUNGRO PADA TANAMAN PADI (Oryza sativa) DI KECAMATAN TABA PENANJUNG : INSIDENSI PENYAKIT DAN DETEKSI VIRUS SECARA MOLEKULER Abdul Fiddin; Mimi Sutrawati; Hendri Bustamam; Dwi Wahyuni Ganefianti; Sipriyadi Sipriyadi
Jurnal Ilmu-Ilmu Pertanian Indonesia Vol 23 No 1 (2021)
Publisher : BPFP Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jipi.23.1.37-45

Abstract

[TUNGRO DISEASE ON RICE PLANT (Oryza sativa) IN TABA PENANJUNG DISTRICT: DISEASE INCIDENCE AND MOLECULAR DETECTION OF VIRUSES]. Tungro is one of the important diseases of rice plants. Tungro disease caused by Rice tungro bacilliform virus (RTBV) and Rice tungro spherical virus (RTSV). This research aims to obtain information on the spread of the disease, vector population, and molecular detection by polymerase chain reaction method. This research was conducted from December 2019 to April 2020 in rice fields in Taba Penanjung sub-district, Bengkulu Tengah district at ± 540 meters above sea level and virus detection in the Laboratory of Biotechnology and Genetics Department of Biology Faculty of Mathematics and Natural Sciences (MIPA) and Laboratory of Plant Protection, Agriculture Faculty, Bengkulu University. Data analysis used descriptive analysis. The results showed that the highest disease incidence on the seven weeks after planting (wap) is 1.7% and the highest population of the vector on the 7 wap with an average of 7.7 vector/100m2 in the village of Taba Penanjung. Detection of viruses with the PCR method with the specific primer DAF/DAR succeeded in amplification of DNA band measuring 1,400 bp. RTSV was not detected by RT- PCR using a specific primer or RTSV. The result of this detection shows that the symptoms of tungro on rice plants in the cultivation of Taba Penanjung district associated with RTBV infection. RTSV unable to detect can be caused by many factors in the detection process, including total RNA extraction, cDNA synthesis, DNA amplification, and DNA visualization of PCR products. 
Co-Authors Abdul Fiddin Dwi Wahyuni Ganefianti Mimi Sutrawati Sipriyadi