Dwi Wahyuni Ganefianti
University of Bengkulu

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Effects of Arbuscular Mycorrhiza Fungi and Coffee Pulp Compost in Improving Soil Water Uptake by Chilli Around the Permanent Wilting Point Conditions Ingri Dayana; Bandi Hermawan; Yudhi Harini Bertham; Dwi Wahyuni Ganefianti
TERRA : Journal of Land Restoration Vol 3, No 1 (2020)
Publisher : Department of Soil Science, Faculty of Agriculture, University of Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (983.959 KB) | DOI: 10.31186/terra.3.1.23-26

Abstract

Soil water availability to the plants is a range of water content between the field capacity and the permanent wilting point (PWP) conditions. The PWP is defined as the lower limit of soil water content that the plant can extract water from the soil as indicated by the symptoms of wilting plants. This is because plant roots are unable to penetrate the soil micropores that contain the water.  The study aims to analyze the effects of arbuscular mycorrhizal fungi (AMF) and compost in enhancing soil water absorption by the plant when the water content is close to the permanent wilting point. Four doses of AMF (0, 5, 10 and 15 g.plant-1) and three doses of coffee pulp-made compost (0, 5 and 10 ton.ha-1) were arranged according to a randomized complete block design with three replicates. Results showed that the application of AMF significantly enabled the plant to improve water uptake when the soil water content was about at the permanent wilting point conditions. The AMF addition of 15 g.plant-1 significantly prolonged the growing period of chili to wither and the plant showed the wilting symptoms at the soil water content of 5 to 7% lower than the no-AMF plants. Improved water uptake under water stress conditions was attributed to increases in the root colonization by AMF.
Relationship Characteristics Soil, Fertilization and Outcome of Rice in Village Lubuk Pinang, Mukomuko Weri Akmaldi; M. Faiz Barchia; Dwi Wahyuni Ganefianti
TERRA : Journal of Land Restoration Vol 3, No 1 (2020)
Publisher : Department of Soil Science, Faculty of Agriculture, University of Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (977.344 KB) | DOI: 10.31186/terra.3.1.15-22

Abstract

This study aims to determine the close relationship between the characteristics of paddy soils and rice yields in Lubuk Pinang, the Manjunto Air Irrigation area, and to determine the level of applied technology of farmers and rice yields in Lubuk Pinang, the Air Manjunto Irrigation area. This research was conducted in April 2018 until June 2018 in Lubuk Pinang, Lubuk Pinang District, Mukomuko Regency. The study was conducted by survey method. Data as collected through direct observation in the field and soil analysis at the Soil Science Laboratory of Bengkulu University. The results showed that the coefficient regression soil pH (X1 ) is 0.208 to 0.875 sig t values are not real in the statistics. The regression coefficient cation exchange capacity (X2 ) is -0.018 with t sig value 0,781. Regression coefficient peat depth (X3 ) is -0.002 to 0.814 sig t values are not real in the statistics. Regression coefficient levels of soil organic C (X4 ) is -0.507 to 0.307 sig t values are not real in the statistics.
PENYAKIT TUNGRO PADA TANAMAN PADI (Oryza sativa) DI KECAMATAN TABA PENANJUNG : INSIDENSI PENYAKIT DAN DETEKSI VIRUS SECARA MOLEKULER Abdul Fiddin; Mimi Sutrawati; Hendri Bustamam; Dwi Wahyuni Ganefianti; Sipriyadi Sipriyadi
Jurnal Ilmu-Ilmu Pertanian Indonesia Vol 23 No 1 (2021)
Publisher : BPFP Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jipi.23.1.37-45

Abstract

[TUNGRO DISEASE ON RICE PLANT (Oryza sativa) IN TABA PENANJUNG DISTRICT: DISEASE INCIDENCE AND MOLECULAR DETECTION OF VIRUSES]. Tungro is one of the important diseases of rice plants. Tungro disease caused by Rice tungro bacilliform virus (RTBV) and Rice tungro spherical virus (RTSV). This research aims to obtain information on the spread of the disease, vector population, and molecular detection by polymerase chain reaction method. This research was conducted from December 2019 to April 2020 in rice fields in Taba Penanjung sub-district, Bengkulu Tengah district at ± 540 meters above sea level and virus detection in the Laboratory of Biotechnology and Genetics Department of Biology Faculty of Mathematics and Natural Sciences (MIPA) and Laboratory of Plant Protection, Agriculture Faculty, Bengkulu University. Data analysis used descriptive analysis. The results showed that the highest disease incidence on the seven weeks after planting (wap) is 1.7% and the highest population of the vector on the 7 wap with an average of 7.7 vector/100m2 in the village of Taba Penanjung. Detection of viruses with the PCR method with the specific primer DAF/DAR succeeded in amplification of DNA band measuring 1,400 bp. RTSV was not detected by RT- PCR using a specific primer or RTSV. The result of this detection shows that the symptoms of tungro on rice plants in the cultivation of Taba Penanjung district associated with RTBV infection. RTSV unable to detect can be caused by many factors in the detection process, including total RNA extraction, cDNA synthesis, DNA amplification, and DNA visualization of PCR products.