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All Journal Jurnal Sain Peternakan Indonesia Tropical Animal Science Journal
W. M. Nalley
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Agriculture, Biological Sciences & Forestry Energy Veterinary

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Kualitas Semen Cair Babi Duroc dalam Pengencer Durasperm yang Disuplementasi Air Buah Lontar dan Sari Tebu Banamtuan, A. N.; Nalley, W. M.; Hine, T. M.
Jurnal Sain Peternakan Indonesia Vol 16 No 1 (2021)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jspi.id.16.1.41-48

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh suplementasi air buah lontar (AbL) dan sari tebu (ST) dalam pengencer  durasperm terhadap kualitas semen cair babi duroc. Semen berasal dari tiga ekor babi jantan Duroc berumur dewasa yaitu  2-3 tahun dan dikoleksi dengan menggunakan glove hand method dua kali seminggu. Semen dievaluasi secara makroskopis dan mikroskopis, semen yang berkualitas baik dibagi menjadi tiga bagian dan dikenakan perlakuan: durasperm (PO), durasperm + AbL (P1), durasperm + ST (P2), selanjutnya disimpan dalam kotak Styrofoam bersuhu 18o - 20 oC. Pengamatan dilakukan setiap delapan jam sekali. Data yang diperoleh dianalisis menggunakan Analisis of Variance (ANOVA) dengan menggunakan program SPSS 20. Hasil penelitian menunjukkan bahwa persentase motilitas pada jam ke 64 menunjukan P1 40% lebih tinggi (P<0,05) daripada P2 (35.00±1.82) dan P0 (27.50±2.88). P1 memiliki persentase viabilitas spermatozoa 50.94±0.79 lebih tinggi (P<0,05) daripada P2 (45.50±1.94) dan P0 (37.75±3.48). P1 memiliki MPU 52.92±0.61 lebih tinggi dengan P2 (47.38±1.47) dan P0 (40.49±3.47). Abnormalitas dan pH tidak ada perbedaan nyata (P?0,05). Disimpulkan bahwa pengencer suplementasi air buah lontar (P1) efektif untuk mempertahankan motilitas, viabilitas, Abnormalitas, MPU dan menjaga pH tetap stabil.
Synergistic Impact of Cholesterol-Loaded Cyclodextrin and Moringa Leaf Extract on Post-Thaw Boar Sperm Kinematics Hine, T. M.; Nalley, W. M.; Uly , K.; Kune , P.; Lawa , A. B.; Setyani , N. M. P.; Bette , Y. Y.; Banamtuan , A. N.; Riwu , R. M. J.
Tropical Animal Science Journal Vol. 49 No. 1 (2026): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2026.49.1.30

Abstract

Structural and functional damage during cryopreservation usually impairs the quality of boar sperm, especially post-thaw motility and kinematic performance, which restricts its use in artificial insemination programs. Cholesterol-loaded cyclodextrin (CLC) and moringa leaf extract (MLE) are considered possible cryoprotective additives because they are membrane-stabilizing and antioxidants. The study investigated the possible synergistic effect of the Tris-egg yolk (TY) extender in combination with CLC and MLE on the motility and kinematics properties of thawed boar sperm, as identified in a Computer-Assisted Sperm Analysis (CASA) system. Ejaculates of four healthy boars were diluted with Tris-based extender without or with CLC (1 or 2 mg/mL), MLE (1 or 1.5 mg/mL), or both, and frozen with a conventional protocol. Kinematic variables alongside progressive and fast motility were included as post-thaw tests. The findings showed that combination treatment of 2 mg/mL CLC and 1 mg/mL MLE (T7) resulted in a significant increase in all sperm motility and kinematic variables compared to the control group (T0) (p<0.05). In particular, T7 had better progressive motility (38.04%), fast motility (22.42%), and higher kinematic variables, especially VCL (106.34 µm/s), VSL (36.28 µm/s), and VAP (45.41 µm/s), and better displacement and trajectory indices. T7 was always better than the single supplementation and other combinations. Finally, the addition of CLC (2 mg/mL) and MLE (1 mg/mL) into the Tris egg yolk extender could be a successful approach towards optimizing semen cryopreservation in boars.
Co-Authors Adyanto Nessy Banamtuan Bette , Y. Y. Kune , P. Lawa , A. B. Riwu , R. M. J. Setyani , N. M. P. Thomas Mata Hine Uly , K.