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Natural honey supplementation in Tris-egg yolk diluent as Carbohydrate source replacing Fructose in Etawah Crossbred Goat semen Yustiani Yuliana Bette; Wilmientje Marlen Nalley; Thomas MataHine
JURNAL ILMIAH PETERNAKAN TERPADU Vol 12, No 1 (2024)
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jipt.v12i1.p49-62

Abstract

This study aims to identify the substitution of native honey as an energy source to replace fructose in Tris-KT diluent to improve the quality of spermatozoa of Etawah crossbreed goats (PE). The study was conducted at the Nekamese Vocational School and the Wiliams and Laura Foundation Biological and Reproductive Laboratory for 2 months. In this study, four PE male goats were used as a source of semen. Cement is accommodated twice a week. The semen obtained was evaluated macroscopically and microscopically then diluted with T-KT diluent with the addition of honey divided into P0 (control), P1 (T-KT + 0.25 w/v honey), P2 (T-KT + 0.50 w/v honey) and P3 (T-KT + 0.75 w/v honey). After dilution the cement is stored at a temperature of 3-5°C.  Semen is evaluated post-dilution and post-storage every 24 hours, until the movement of spermatozoa is at least 40%. The variables tested were motility and viability of spermatozoa. P3 provides the best results in maintaining cement quality. The results showed that honey substitution in Tris-KT diluent exerted a marked effect on the motility and viability of spermatozoa (P<0.05) during storage.
Effect of Fructose Levels in Diluents Citric Acid Egg Yolk on The Quality of Liquid Boar Semen Landrace Mallo, Agustinus; Nalley, Wilmientje Marlene; Bette, Yustiani Yuliana
JURNAL ILMIAH PETERNAKAN TERPADU Vol 12, No 3 (2024)
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The aim of the research was to test the addition of fructose (F) in egg yolk citrate diluent (C-EY) on the quality of liquid semen from Landrace pigs during storage. This research used a completely randomized design method consisting of five treatments and five replications. The treatments in question are P0 (0%F), P1 (0.5%F), P2 (1.0%F), P3 (1.5%F) and P4 (2.0%F). The diluted cement is stored at a temperature of 18-20oC. Evaluation of the motility, viability, abnormalities and survival of spermatozoa is carried out every twelve hours. Analysis of variance (ANOVA) of data using SPSS. 25 for windows. The results showed P3 at 12 hours (80.00 ± 0.00%), 24 hours (74.00 ± 2.24%), 36 hours (62.00 ± 2.74%), 48 hours (41.00 ±2.24%) and spermatozoa were able to survive 48 hours of storage. P3 gave the best results (P<0.05) compared to other treatments with motility values of 41.00 ± 2.24%, viability 45.20 ± 1.64%, abnormalities 8.20 ± 1.10% and survival 51.20±3.05 hours. It was concluded that the addition of 1.5% fructose level in the egg yolk citrate diluent could maintain the quality of liquid semen longer than other treatments.
PENINGKATAN KUALIAS PAKAN DENGAN PEMANFAATKAN DAUN KELOR DALAM MENINGKATKAN PRODUKTIVITAS TERNAK David Agustinus Nguru; Alberth N. Ndun; Alvrado B. Lawa; Simon E. Mulik; Salden E. Nifu; Yustiani Y. Bette
JMM (Jurnal Masyarakat Mandiri) Vol 8, No 6 (2024): Desember
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jmm.v8i6.27198

Abstract

Abstrak: Ketertarikan masyarakat Nusa Tenggara Timur dalam beternak babi sejalan dengan meningkatnya permintaan akan daging babi. Namun, produktivitas ternak babi belum mampu memenuhi permintaan daging babi. Tingginya harga pakan berdampak pada cara peternak memberikan pakan, yang sering kali tidak sesuai dengan kebutuhan harian ternak babi. Kegiatan PKM (Pengabdian Kepada Masyarakat) bertujuan untuk mendukung peternak dalam memenuhi kebutuhan harian ternak babi dengan memanfaatkan bahan lokal, serta meningkatkan keterampilan softsskill dan hardskill jemaat GMIT Diaspora Danau Ina Lasiana. Pelaksanaan Pengabdian Kepada Masyarakat (PKM) ini dilakukan dengan metode ceramah serta eksperimen langsung yang melibatkan jemaat. Kegiatan ini dihadiri oleh 25 jemaat, terdiri dari 20 laki-laki dan 5 wanita. Evaluasi terhadap kegiatan ini dilakukan dengan menggunakan pre-test yang terdiri sari 15 soal, yang dilaksanakan sebalum kegiatan dan post-test dilaksanakan setalah kegiatan berakhir. Tujuan dari program PKM ini yaitu untuk membantu jemaat dalam memenuhi kebutuhan harian ternak babi dengan memanfaatkan daun kelor. Hasil evaluasi post-test yang dilakukan pada 25 orang menunjukkan bahwa 80% responden mengalami peningkatan pengetahuan tentang pemanfaatan bahan lokal dalam memenuhi kebutuhan harian ternak babi.Abstract: The interest of the people of East Nusa Tenggara in raising pigs is in line with the increasing demand for pork. However, the productivity of pig farming has not been able to meet the demand for pork. High feed prices have an impact on the way farmers provide feed, which often does not match the daily needs of pigs. PKM (Community Service) activities aim to support farmers in meeting the daily needs of pigs by utilizing local ingredients, as well as improving the soft skills and hard skills of the GMIT Diaspora Lake Ina Lasiana congregation. The implementation of Community Service (PKM) is carried out by lecture method and direct experiments involving congregations. This activity was attended by 25 congregations, consisting of 20 men and 5 women. The evaluation of this activity was carried out using a pre-test consisting of 15 questions, which was carried out throughout the activity and post-test was carried out after the activity ended. The purpose of this PKM program is to help the congregation in meeting the daily needs of pigs by utilizing moringa leaves. The results of the post-test evaluation conducted on 25 people showed that 80% of the respondents experienced an increase in knowledge about the use of local ingredients in meeting the daily needs of pig farming.
EFEK BERBAGAI KOMBINASI FRUKTOSA DAN SUKROSA DALAM PENGENCER SITRAT KUNING TELUR TERHADAP KUALITAS SEMEN CAIR BABI LANDRACE: Impact of Different Combinations of Fructose and Sucrose in Citrate Egg Yolk Diluents on the Quality of Landrace Boar Liquid Semen George Valentino Kosat; Thomas Mata Hine; Yustiani Yuliana Bette
Wahana Peternakan Vol. 9 No. 2 (2025): Wahana Peternakan
Publisher : Faculty of Animal Science, University of Tulang Bawang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37090/jwputb.v9i2.2329

Abstract

Tujuan penelitian adalah untuk mengkaji efek berbagai kombinasi fruktosa dan sukrosa dalam pengencer sitrat-kuning telur (SKT) terhadap kualitas semen cair babi landrace. Rancangan penelitian menggunakan rancangan acak lengkap terdiri dari lima perlakuan yaitu S-KT (P0), S-KT + 1,0% fruktosa + 0,2% sukrosa (P1), S-KT + 1,5% fruktosa + 0,2% sukrosa (P2), S-KT + 1,0 % fruktosa + 0,4% sukrosa (P3), S-KT + 1,5% fruktosa + 0,4% sukrosa (P4), setiap perlakuan diulang lima kali. Semen yang berkualitas baik diencerkan dengan pengencer, kemudian dipreservasi dengan suhu 15-20oC. Evaluasi dilakukan setiap 12 jam. Hasil pengamatan pada jam ke-60 preservasi menunjukkan bahwa perlakuan P2 menghasilkan kualitas spermatozoa tertinggi dan berbeda secara nyata (P<0,05) dengan perlakuan kontrol (P0) pada variabel motilitas, viabilitas, dan daya tahan hidup spermatozoa, namun berbeda secara tidak signifikan (P>0,05) pada variabel abnormalitas spermatozoa. Perbandingan kualitas semen cair babi landrace antara perlakuan P2 dan P0 adalah motilitas spermatozoa (44 vs 31%),viabilitas spermatozoa (59,60 vs 52,20%), abnormalitas spermatozoa (11,80 vs 11,80%), dan daya tahan hidup spermatozoa (63,80 vs 51,20 jam). Disimpulkan bahwa kombinasi fruktosa 1,5% dan sukrosa 0,2% dalam pengencer sitrat kuning telur menghasilkan kualitas semen cair tertinggi dibandingkan dengan perlakuan kontrol. Kata Kunci : Fruktosa, Kualitas Semen Cair Babi Landrace, Sitrat-Kuning Telur, Sukrosa.
Natural honey supplementation in Tris-egg yolk diluent as Carbohydrate source replacing Fructose in Etawah Crossbred Goat semen Bette, Yustiani Yuliana; Nalley, Wilmientje Marlen; MataHine, Thomas
JURNAL ILMIAH PETERNAKAN TERPADU Vol. 12 No. 1 (2024)
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jipt.v12i1.p49-62

Abstract

This study aims to identify the substitution of native honey as an energy source to replace fructose in Tris-KT diluent to improve the quality of spermatozoa of Etawah crossbreed goats (PE). The study was conducted at the Nekamese Vocational School and the Wiliams and Laura Foundation Biological and Reproductive Laboratory for 2 months. In this study, four PE male goats were used as a source of semen. Cement is accommodated twice a week. The semen obtained was evaluated macroscopically and microscopically then diluted with T-KT diluent with the addition of honey divided into P0 (control), P1 (T-KT + 0.25 w/v honey), P2 (T-KT + 0.50 w/v honey) and P3 (T-KT + 0.75 w/v honey). After dilution the cement is stored at a temperature of 3-5°C.  Semen is evaluated post-dilution and post-storage every 24 hours, until the movement of spermatozoa is at least 40%. The variables tested were motility and viability of spermatozoa. P3 provides the best results in maintaining cement quality. The results showed that honey substitution in Tris-KT diluent exerted a marked effect on the motility and viability of spermatozoa (P<0.05) during storage.
Effect of Fructose Levels in Diluents Citric Acid Egg Yolk on The Quality of Liquid Boar Semen Landrace Mallo, Agustinus; Nalley, Wilmientje Marlene; Bette, Yustiani Yuliana
JURNAL ILMIAH PETERNAKAN TERPADU Vol. 12 No. 3 (2024)
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jipt.v12i3.p341-353

Abstract

The aim of the research was to test the addition of fructose (F) in egg yolk citrate diluent (C-EY) on the quality of liquid semen from Landrace pigs during storage. This research used a completely randomized design method consisting of five treatments and five replications. The treatments in question are P0 (0%F), P1 (0.5%F), P2 (1.0%F), P3 (1.5%F) and P4 (2.0%F). The diluted cement is stored at a temperature of 18-20oC. Evaluation of the motility, viability, abnormalities and survival of spermatozoa is carried out every twelve hours. Analysis of variance (ANOVA) of data using SPSS. 25 for windows. The results showed P3 at 12 hours (80.00 ± 0.00%), 24 hours (74.00 ± 2.24%), 36 hours (62.00 ± 2.74%), 48 hours (41.00 ±2.24%) and spermatozoa were able to survive 48 hours of storage. P3 gave the best results (P<0.05) compared to other treatments with motility values of 41.00 ± 2.24%, viability 45.20 ± 1.64%, abnormalities 8.20 ± 1.10% and survival 51.20±3.05 hours. It was concluded that the addition of 1.5% fructose level in the egg yolk citrate diluent could maintain the quality of liquid semen longer than other treatments.
Success Rate Of Artificial Insemination Using Honey In Tris-Egg Yolk Dilution In Peanut Goats Bette, Yustiani Yuliana; Nalley, Wilmientje Marlene
JURNAL ILMIAH PETERNAKAN TERPADU Vol. 13 No. 1 (2025)
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jipt.v13i1.p98-108

Abstract

Artificial Insemination (AI) is a technique used to improve the genetic quality and quality of livestock. This study aims to determine the success rate of Artificial Insemination using honey as a Tris-Egg Yolk diluent in peanut goats. Two PE male goats aged 2-3 years as semen sources and 18 peanut goats as acceptor females aged 1-3 years have already given birth and are showing an estrus cycle. Acceptor females were divided into two groups with two replications. group 1, insemination using Tris-KT diluent without honey and Group 2 with Tris-KT+Md diluent 0.75 v/w. The semen used for insemination was liquid semen with a dose of 0.2 ml / head with a concentration of 3.89 ± 0.46 x 109 cells / ml and viability not below 50%. Data were analyzed using the Chi square (X)² test to.determine the effect of treatment on the Non Return Rate (NRR) value parameters for 3 estrus cycles after AI and the Conception Rate (CR) value based on observations of 2 month pregnancy. The results showed that there was no real difference between treatments (P>0.05) in pregnancy rates with NRR and CR values of 77.78% (P1) and 88.88% (P2).