Article Per Year (5 Year)
p-Index From 2021 - 2026
0.444
P-Index
This Author published in this journals
All Journal Jurnal AgroBiogen Jurnal Penelitian Tanaman Industri Plantropica: Journal of Agricultural Science AGRIVITA, Journal of Agricultural Science Kultivasi BUANA SAINS Jurnal Penelitian Tanaman Industri (Littri)
Darmawan Saptadi
Department Of Agronomy, Faculty Of Agriculture, Universitas Brawijaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Industrial & Manufacturing Engineering Mathematics Physics

Published : 10 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search
Journal : Jurnal AgroBiogen

 1 
Genetic Diversity Analysis and Development of DNA Fingerprints of 20 Indonesian Local Chili Pepper Varieties Based on SSR Markers Rerenstradika Tizar Terryana; Nadia Della Savitri Ayu Ningrum; Kristianto Nugroho; Darmawan Saptadi; Helmi Kurniawan; Puji Lestari
Jurnal AgroBiogen Vol 16, No 2 (2020): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v16n2.2020.p45-58

Abstract

Chili pepper is one of the most valuable horticultural crops, widely cultivated in Indonesia. Analysis of its genetic diversity is needed to develop successful breeding programs of local varieties. Simple sequence repeat (SSR), a robust molecular marker used for genetic diversity analysis in plant species, offers potential, reliable DNA fingerprinting method to assess genetic variation and varietal identification of chili pepper. Fifteen SSR markers were used in this study to analyze the genetic diversity and develop profiling identification of DNA fingerprint of local chili pepper varieties. Twenty local and two improved varieties of three chili pepper species, consisting of 3, 1, and 18 varieties of Capsicum frutescens, C. chinense, and C. annuum, respectively, were assessed for their SSR polymorphism. A total of 87 alleles was obtained from the polymorphism analysis with high alleles variation (2–16 alleles) with average total allele of 5.8 and average polymorphism information content (PIC) of 0.59 (0.34–0.83). Clustering and Principle Coordinate Analyses (PCoA) classified the varieties into two groups with coefficient of similarity of 0.65 indicating their high genetic variability. Most local varieties belonged to the same cluster and separated from the two improved varieties. Based on PIC values and dendrogram with selected markers, five SSR markers, i.e. EPMS441, EPMS331, EPMS335, GPMS194, and CaSSRBio1.1, were identified as SSR marker set for DNA fingerprinting purposes. SSR marker set used in this study was successful in developing the varietal identity of local chili pepper varieties, as indicated by unique code of each variety.
Confirmation of Alleles Inheritance in F1 Progenies Derived from a Cross of Calcutta-4 (Musa acuminata ssp. burmannicoides) and Musa acuminata ssp. microcarpa Based on SSR Markers Dea Rosalia; Puji Lestari; Andy Soegianto; Darmawan Saptadi; Agus Sutanto; Kristianto Nugroho; Rerenstradika T. Terryana; I Made Tasma; Ika Roostika
Jurnal AgroBiogen Vol 16, No 1 (2020): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v16n1.2020.p17-24

Abstract

Banana breeding to produce improved varieties with disease resistance haracters and other desired traits could sustain its yield. Alleles harbored by parents could be passed on to the offsprings through hybridization, but need to be confirmed using molecular markers. This study aimed to confirm allele inheritance in F1 progenies derived from a cross of Calcutta-4 (Musa acuminata ssp.  burmannicoides) and M. acuminata ssp. microcarpa based on SSR markers. Eleven pairs of SSR primers were used to amplify DNA of 44 progenies using the PCR technique. The results showed that six SSR markers (MaSSR 1.1, MaSSR5.1, MaSSR 6.1, MaSSR 7.1, MaSSR 8.1, and MaSSR 11.1) were polymorphic in both parents. Four markers (MaSSR 1.1, MaSSR 5.1, MaSSR 6.1, and MaSSR 8.1) had PIC >0.7, indicating their informativeness to distinguish these progenies and other genetic studies of banana germplasms. A total of 44 F1 individuals were confirmed to harbor alleles inherited from their parents,suggesting as true progenies from the cross of Calcutta-4 and M. acuminata ssp. microcarpa. This population demonstrated 100% success of hybridization performed. Chi-Square analysis revealed that segregation of all markers did not match to Mendelian ratio 1:2:1, except for MaSSR 1.1 (x2 = 5,62) and MaSSR 6.1 (x2 = 3,77) markers. The genetic traceability of banana F1 progenies demonstrating the usefulness and feasibility of SSR markers in this study provided information on selection of true progenies which may be valuable for breeders to assist selection process in future banana breeding program in Indonesia.
Co-Authors Agus Sutanto Andy Soegianto Anis Andrini BAMBANG HELIYANTO Budi Waluyo Damanhuri Damanhuri Dea Rosalia Descha Giatri Cahyaningrum Diana Rizky Amalia Freta Kirana Balladona Gita Novita Sari Helmi Kurniawan I Made Tasma Ika Roostika Kristianto Nugroho Kristianto Nugroho Kuswanto Kuswanto Lazuardi Pramadio Nadia Della Savitri Ayu Ningrum Noer Rahmi Ardiarini, Noer Rahmi Puji Lestari Puji Lestari R.R. SRI HARTATI Rerenstradika T. Terryana Rerenstradika Tizar Terryana Sandra Wicaksono Setiawan, Asep S SUDARSONO SUDARSONO