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All Journal AGRIVITA, Journal of Agricultural Science Jurnal Ilmu dan Teknologi Kayu Tropis
Sigit Sunarta
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Agriculture, Biological Sciences & Forestry

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Antifungal Activity of Eucalyptus urophylla Oil Against Aspergillus niger and Fusarium oxysporum Rini Pujiarti; Handojo Hadi Nurjanto; Sigit Sunarta
AGRIVITA, Journal of Agricultural Science Vol 40, No 1 (2018): FEBRUARY
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v40i1.990

Abstract

Essential oils obtained from Eucalyptus posses many bioactivities as fungicidal, antimicrobial, insecticidal and other activities. This study elucidated chemical compounds and antifungal activity of Eucalyptus urophylla leaves oil. Effectiveness of E. urophylla leaves oil were evaluated as antifungal against pathogenic fungi of A. niger and F. oxysporum. Eucalyptus urophylla oil was obtained by hydrodistillation method from fresh leaves of E. urophylla. Gas chromatography-mass spectrometry (GC-MS) analysis was used to analyze the chemical compounds of E. urophylla oil. Antifungal activity of E. urophylla oil was tested with in-vitro assay against A. niger and F. oxysporum strains with five levels of oil concentration (1 mg ml-1; 5 mg ml-1; 10 mg ml-1; 15 mg ml-1; 20 mg ml-1). GC-MS analysis showed the most abundant compounds of E. urophylla oil were 1,8-cineole (66.31 %), α-pinene (16.92 %), α-terpinyl acetate (6.00 %) and γ-terpinene (5.13 %). Antifungal assay showed inhibitory effects of E. urophylla against F. oxysporum with IC50 = 1.61 mg ml-1 and A. niger with IC50 = 13.56 mg ml-1. Antifungal activity of E. urophylla oil in this study was probably due to the presence of 1,8-cineole. Results indicated the possibility of E. urophylla as antifungal against F. oxysporum and A. niger.
Evaluasi terhadap Stimulan Ethephon dalam Penyadapan Pinus merkusii (Evaluation on Ethephon Stimulant to Pinus merkusii Tapping) Ganis Lukmandaru; Sigit Sunarta; Tomy Listyanto; Kasmudjo Kasmudjo; Rini Pujiarti; Ragil Widyorini
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 16, No 2 (2018): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1355.764 KB) | DOI: 10.51850/jitkt.v16i2.454

Abstract

The aim of this study was to find out the effect of Ethephon, an ethylene releasing compound, in a combination with sulfuric acid resin production Pinus merkusii.  The tested stimulants were ETRAT; SR4; 20% sulfuric acid; 20% sulfuric acid - Ethephon 2%; 3.3% sulfuric acid – 4.1% Ethephon; and sulfuric acid – 8.3% Ethephon. Trees without stimulant spraying were the controls. The results showed that for daily production, the first-day observation gave the highest value but decreased drastically after second-day observation in a varied degree depent on the stimulants. On the basis of accumulated production, the addition of Ethephon in a high or low level of sulfuric acid concentration exhibited significantly higher resin production compared to that of ETRAT; SR4; and 20% sulfuric acid stimulants as well as control trees. It increased resin production by 98.0-150.6% compared to control trees in class age of VII and increased by 140.9-227.1% in class age of IV stands. Moderate significant correlations (r=0.65) were observed between the values of resin production and tree diameter (controls) as well as between the values of resin production and tree diameter of trees treated by sulfuric acid stimulants (r=0.65-0.82).
Co-Authors Ganis Lukmandaru Handojo Hadi Nurjanto Kasmudjo Kasmudjo Ragil Widyorini Rini Pujiarti Rini Pujiarti Tomy Listyanto