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Priming Low-Dose Gamma Irradiation Increases Cellular Radioadaptation Response through the Induction of Hsp70 and SOD2 S. Supriyadi
Atom Indonesia Vol 47, No 2 (2021): August 2021
Publisher : PPIKSN-BATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17146/aij.2021.1019

Exposure to low-dose radiation has been demonstrated to stimulate increased cell protection when receiving subsequent challenge dose in what is known as radioadaptation response. Hsp70 and SOD, especially SOD2, are cytoprotectors against superoxide radicals generated by radiation exposure. This study aims to measure the expressions of Hsp70 and SOD2 in parotid salivary gland acinar cells as an indicator of radioadaptation response stimulated by low-dose gamma irradiation. The study used 24 male Rattus norvegicus that are divided into four groups: normal control, positive control, with 50-mGy priming irradiation, and with 100-mGy priming irradiation. The animals were immobilized without anesthetics with special tools designed especially for this study. Irradiation was carried out using a cobalt-60 (gamma ray) teletherapy unit (Philips XK-100) directed to the dorsa of the animals’ heads. High-dose gamma irradiation (2 Gy) was administered 5 hours after priming irradiation. The expression of Hsp70 and SOD2 was measured through immunohistochemical technique on the parotid salivary gland acinar cells and observed using a light microscope with 1000× magnification. Data obtained was analyzed with one-way ANOVA test (α = 0.05). The results showed that Hsp70 and SOD2 expressions in the priming irradiation groups were higher than those in control groups. The conclusion of this study: priming irradiation with low-dose gamma radiation before challenge irradiation with high-dose gamma radiation increases the radioadaptation response of salivary gland acinar cells through induction of Hsp70 and SOD2.

Efektivitas air perasan pulpa kakao 50% dalam membersihkan smear layer pada dinding saluran akar gigi The effectiveness of 50% cocoa pulp juice on removing smear layer of root canal dentin Disya Dwi Maulidiyah; Sri Lestari; Raditya Nugroho; Supriyadi Supriyadi; Dwi Warna Aju Fatmawati
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 33, No 3 (2021): Desember 2021
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkg.v33i3.34435

Pendahuluan: Smear layer merupakan lapisan tipis berisi substansi organik dan anorganik yang keberadaannya dapat menghalangi penetrasi medikamen ke tubuli dentin, sehingga harus dihilangkan dengan bahan irigasi saluran akar yaitu EDTA 17%. Bahan alami yang dapat digunakan sebagai alternatif dalam membersihkan smear layer pada dinding saluran akar adalah air perasan pulpa kakao 50% karena mengandung berbagai senyawa asam dan saponin. Tujuan penelitian menganalisis kemampuan air perasan pulpa kakao (Theobroma cacao L.) 50% dalam membersihkan smear layer pada dinding saluran akar Metode: Penelitian ini merupakan penelitian experimental laboratoris dengan rancangan penelitian the posttest only control group design. Terdapat dua kelompok uji yaitu air perasan pulpa kakao 50% dan EDTA 17%, masing-masing adalah 4 buah. Sampel yang digunakan adalah gigi insisif sapi yang direndam dalam larutan salin. Sampel dipotong mahkotanya setinggi servikal gigi dan di preparasi saluran akarnya dengan teknik konvensional menggunakan file no 45-80 dengan panjang kerja 21 mm, dirigasi dengan air perasan pulpa kakao 50% dan EDTA 17% tiap pergantian instrumen. Bagian yang diamati dengan Scanning Electron Microscope (SEM) 2000x adalah 1/3 tengah akar gigi bukal. Hasil pemotretan SEM dilakukan perhitungan skor menurut sistem skoring Hülsmann dan dilakukan analisis data dengan uji statistik Mann Whitney U Test. Hasil: Hasil penelitian dan analisis data didapatkan p=0,495 yang menunjukan bahwa tidak terdapat perbedaan bermakna antara keduanya. Kedua kelompok memiliki rerata skor 2 yang artinya sebagian orifis tubuli dentin terbuka dan terdapat sedikit smear layer. Simpulan: Air perasan pulpa kakao 50% mampu membersihkan smear layer saluran akar yang sebanding/setara dengan EDTA 17%.Kata kunci: air perasan pulpa kakao; bahan irigasi saluran akar; smear layerABSTRACTIntroduction: Smear layer is a thin layer containing organic and inorganic substances whose presence can prevent the penetration of the medicament into the dentinal tubules, so it must be removed with root canal irrigation material, namely 17% EDTA. Natural ingredients that can be used to clean the smear layer on the root canal wall are 50% cocoa pulp juice because it contains various acidic compounds and saponins. Therefore, the study aimed to analyze the ability of 50% cocoa (Theobroma cacao L.) pulp juice to clean the smear layer on the root canal walls. Methods: This study was an experimental laboratory study with the posttest only control group design. There were two test groups, namely 50% cocoa pulp juice and 17% EDTA, which were four pieces. The sample used was a bovine incisor immersed in a saline solution. The samples were crowned at the teeth' cervical level, and the root canals were prepared using conventional techniques using file no. 45-80 with a working length of 21 mm, irrigated with 50% cocoa pulp juice and 17% EDTA at each instrument change. The part observed with a Scanning Electron Microscope (SEM) 2000x was the middle 1/3 of the buccal tooth root. The results of the SEM observation were calculated according to the Hülsmann scoring system, and data were analyzed using the Mann Whitney U Test statistical test. Results: The results of the study and data analysis obtained p = 0.495, which indicates that there is no significant difference between the two. Both groups had a mean score of 2, meaning that most of the dentinal tubular orifices are open, and there is a thin smear layer. Conclusions: 50% cocoa pulp juice was able to clean the root canal smear layer, equivalent to 17% EDTA.Keywords: cocoa pulp juice; root canal irrigation agents; smear layer

PEDOMAN INTERPRETASI RADIOGRAF LESI-LESI DI RONGGA MULUT Supriyadi Supriyadi
STOMATOGNATIC - Jurnal Kedokteran Gigi Vol 9 No 3 (2012)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

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The interpretation of dental radiograph was be important to support of diagnose a specially was diagnose of oral lessions. The purpose of this paper was to give of dental radiograph interpretation guidens so can to improve the accuracy of diagnose. The guidens of dental radiograph was to enclosed: 1) the meaning and objective of of dental radiograph interpretation,2) the commonly principle of dental radiograph interpretation, 3) the systematic procedure of dental radiograph interpretation, and 4) the differential diagnose steps of oral lessions

PENURUNAN JUMLAH ERITROSIT DARAH TEPI AKIBAT PAPARAN RADIASI SINAR X DOSIS RADIOGRAFI PERIAPIKAL Erma N.K; Supriyadi Supriyadi
STOMATOGNATIC - Jurnal Kedokteran Gigi Vol 9 No 3 (2012)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

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The purpose of this study was to evaluate the decreasing of erythrocyte cappilary blood vessel on X-ray radiation exposure with periapicale radiography dosage. This study is laboratory experimental research that used the Post-Test Control Group Design. The sample research was mus musculus, male, 3–4 month of age, 20– 25 g of weight and healthy. There were 24 mus musculus that were divided into 6 groups wich each group 6 mus musculus: the first group was used as a control group (no X-ray radiation exposure), the second group was induced with X-ray radiation with once exposure of periapicale radiography dosage. The third group was induced with X-ray radiation with six- time exposure of periapicale radiography with 1 minute of interval. The fourth group was induced with X-ray radiation with fourteen-time exposure of periapicale radiography dosage with 1 minute of interval. The source of radiation was the X-ray radiation exposure was given from dental radiography unit (thropy) : periapicale projection, 70 Kvolt voltage, 8 mA, 20 cm of Source Object Distance (SOD), adult patient type and molar region selector. The entire sample was taken hereinafter sacrificed 24 hours after radiation exposure. The observation and counting of erythrocyte using with erythrocyte counting room. The data were statistically analyzed using one-way anova at significant level (α) of 0.05. The results of this study showed that was decreasing an amount of erythrocyte cappilary blood vessel; at control group was 2,5833 million cell/mm3, at the second group was 2,4167 million cell/mm3, at the third group was 2,3500 million cell/mm3, and the fourth group was 2,0000 million cell/mm3.There was significant decreasing an amount of erythrocyte among the four groups (p < 0.05). There were decreasing an amount of erythrocyte cappilary blood vessel was caused by X-ray radiation induced from periapicale radiography

COUNT OF LEUKOCYTE AFTER EXPOSURE TO LOW DOSE X RAY RADIATION IN MALE WISTAR RATS (RATTUS NORVEGICUS) Afifah Rizki Fauziah; Swasthi Prasetyarini; Supriyadi Supriyadi
UNEJ e-Proceeding 2022: E-Prosiding Kolokium Hasil Penelitian dan Pengabdian kepada Masyarakat
Publisher : UPT Penerbitan Universitas Jember

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Panoramic radiography was one of radiographic examinations in dental practice due to several advantages, which one was for orthodontic treatment. The use of X-ray radiation has biological effects that caused cell death (necrosis or apoptosis). Examination the number of leukocytes can be used as an indicator to determine the responsiveness of white blood cells in preventing the presence of disease and inflammation agents. This research aims to determine the number of leukocytes after low-dose X-ray radiation exposure in male wistar rats (Rattus norvegicus). The methods used experimental laboratory research methods with post test only control group design. A sample of 20 wistar rats were divided into 5 groups: 1 control group and 4 treatment groups that were selected based on inclusion and exclusion criteria. The selected sample was then taken a blood sample before and after low-dose X-ray radiation exposure. Data from the study were conducted parametric statistical tests of One Way Annova and LSD. Radiation exposure used panoramic radiography units under the brand F1-04300 TUUSULA with exposure time 14.1 seconds, and a dose of 10.6 mGy. Examination of the number of leukocytes used the manual method. Data were analyzed using the One Way Anova test with a significant level of α (0.05). Results: The results of the analysis showed that there were significant differences between the control group and the treatment group (α <0.05). Conclusion: There was a change in the number of leukocytes after low-dose X-ray radiation exposure.

Daya antibakteri ekstrak buah okra hijau (Abelmoschus esculentus) terhadap Streptococcus mitis Safrida Nur Islamiah Ika Putri; Sri Lestari; Supriyadi Supriyadi
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 35, No 1 (2023): April 2023
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkg.v35i1.40921

ABSTRAKPendahuluan: Streptococcus mitis (S.mitis) adalah bakteri yang dominan ditemukan pada infeksi saluran akar primer dan sangat berkaitan dengan rasa nyeri. Irigasi merupakan suatu langkah penting dalam mengeliminasi bakteri pada saluran akar yang terinfeksi. Penggunaan NaOCl 2,5% sebagai bahan irigasi memiliki beberapa kekurangan, diantaranya adalah bersifat toksik dan iritatif, sehingga diperlukan alternatif yang lebih aman. Ekstrak buah okra hijau berpotensi untuk digunakan sebagai alternatif karena memiliki kandungan flavonoid, alkaloid, saponin, tanin dan terpenoid yang bersifat antibakteri. Tujuan penelitian ini untuk mengetahui daya antibakteri ekstrak buah okra hijau terhadap pertumbuhan S.mitis. Metode: Jenis penelitian adalah eksperimental laboratoris dengan desain posttest-only control group design. Uji antibakteri dilakukan dengan metode disk diffusion, menggunakan 4 kelompok perlakuan yaitu ekstrak buah okra hijau konsentrasi 1,563, 3,125, 6,25, 12,5 dan NaOCl 2,5%. Hasil penelitian dianalisis menggunakan uji nonparametrik Kruskal Wallis dan Mann Whitney (α=0,05). Hasil: Rerata diameter zona hambat yang terbentuk pada ekstrak buah okra hijau konsentrasi 1,563% (0 mm), 3,125% (0 mm), 6,25% (14,68 mm), 12,5% (18,13 mm) dan NaOCl 2,5% (23,79 mm). Uji Kruskal Wallis menunjukkan nilai p=0,001, artinya terdapat perbedaan antar semua kelompok. Uji Mann Whitney menunjukkan terdapat perbedaan yang signifikan antar semua kelompok penelitian kecuali pada ekstrak buah okra hijau konsentrasi 1,563% dan 3,125%. Simpulan: Ekstrak buah okra hijau mempunyai daya antibakteri terhadap pertumbuhan S.mitis pada konsentrasi 6,25% dan 12,5%, namun antibakteri ini masih lebih rendah dibanding NaOCl 2,5%. Konsentrasi 1,56% dan 3,125% belum memiliki daya antibakteri terhadap pertumbuhan S.mitis.Kata Kunci : Buah okra hijau, streptoccus mitis, daya antibakteri Antibacterial activity of green okra fruit (Abelmoschus esculentus) extract against Streptococcus mitis ABSTRACTIntroduction: Streptococcus mitis (S.mitis) is the predominant bacterium found in primary root canal infections and strongly associated with pain. Irrigation is an important step in eliminating bacteria in infected root canals. The use of 2.5% NaOCl as an irrigant has several disadvantages including being toxic and irritating. Green okra fruit extract has the potential to be used as an alternative because it contains antibacterial properties such as flavonoids, alkaloids, saponins, tannins and terpenoids. This study aimed to determine the antibacterial activity of green okra fruit extract on the growth of S.mitis. Methods: This research is an experimental laboratory research with a posttest-only control group design. Inhibition test was carried out using disk diffusion method which consisted of 4 research groups (green okra fruit extract with a concentration of 1.563%, 3.125%, 6.25%, 12.5% and NaOCl 2.5%). The results were analyzed using nonparametric test Kruskal Wallis and Mann Whitney(α=0,05). Results: The average diameter of the inhibition zone formed in the green okra fruit extract were 1.563% (0 mm), 3.125% (0 mm), 6.25% (14.68 mm), 12.5% (18.13 mm) and NaOCl. 2.5% (23.79 mm). Kruskal Wallis test showed p=0.001, means that there were differences between all groups. Mann Whitney test showed that there were significant differences between all research groups except for the green okra fruit extract at concentrations of 1.563% and 3.125%. Conclusion: Green okra fruit extract had antibacterial activity against the growth of S. mitis at concentrations of 6.25% and 12.5%, but this antibacterial was still lower than NaOCl 2,5%. Concentrations of 1.56% and 3.125%, they did not have antibacterial activity against the growth of S. mitis.Keywords: green okra, fruit extract, streptococcus mitis, antibacterial

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