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PERUBAHAN WARNA RESIN KOMPOSIT NANOFILLER SETELAH PERENDAMAN DALAM MINUMAN SUSU FERMENTASI (PENELITIAN IN VITRO) I, Istibsyaroh; Lestari, Sri; Nugroho, Raditya
The Indonesian Journal of Health Science Vol 10, No 1 (2018): The Indonesian Journal Of Health Science
Publisher : Universitas Muhammadiyah Jember

Resin komposit adalah salah satu jenis bahan tumpatan gigi anterior yang populer karena memiliki kelebihan dalam bidang estetik dan sewarna gigi. Komposit nanofiller memiliki sifat mekanik lebih baik dibandingkan microhybrid dan dapat mempertahankan kehalusan selama pemakaian setara microfiller. Resin komposit dapat mengalami perubahan warna di dalam rongga mulut, salah satunya dapat disebabkan oleh minuman dengan pH rendah. Tujuan penelitian adalah mengetahui adanya pengaruh perendaman di dalam minuman susu fermentasi terhadap perubahan warna resin komposit nanofiller. Selain itu untuk mengetahui adanya perbedaan perubahan warna resin komposit nanofiller selama perendaman 6, 12, dan 18 jam. Jenis penelitian yang digunakan adalah eksperimental laboratories dengan rancangan penelitian desain grup pretest and post test. Sampel berjumlah 24 direndam dalam susu fermentasi selama 6, 12, dan 18 jam. Pengukuran perubahan warna menggunakan spektrofotometer optik untuk mengetahui nilai intensitas cahaya. Nilai perubahan warna tampak pada besarnya nilai intensitas cahaya yang muncul pada alat spektrofotometer optik. Analisis data menggunakan uji Paired-T Test dan One Way Annova. Nilai uji intensitas cahaya sesudah perlakuan lebih tinggi dari sebelum perlakuan. Selisih rata-rata nilai uji intensitas cahaya sebelum dan setellah perlakuan paling tinggi pada perendaman 18 jam dan paling rendah pada perendaman 6 jam. Resin komposit nanofiller mengalami perubahan warna setelah dilakukan perendaman dalam minuman susu fermentasi dengan perbedaan yang signifikan. Kata kunci: Resin Komposit, Perubahan Warna, Estetik, Susu Fermentasi, Nanofiller

Pengaruh Aplikasi Gliserin pada Kekerasan Resin Komposit Nanofiller dengan Perendaman Cuka Apel Raditya Nugroho; Putri Nila Kharisma; Roedy Budirahardjo
Pustaka Kesehatan Vol 8 No 2 (2020): Volume 8 No. 2, 2020
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v8i2.11322

Composite resin is a type of filling material that is often used because it has good bonds with dentine and enamel and has a high aesthetic value. Nanofiller composite resin has high hardness, good polishing, color accuracy, and good translucency. The hardness of composite resins can change caused by low pH beverage. Purpose: To determine the differences in effect of glycerin application on hardness of nanofiller composite resins with immersion of apple cider vinegar. The type of research used was experimental laboratories with a pretest and post test group design. Samples were 24 immersion in apple vinegar for 60 and 120 minutes. Measurement of hardness using the Vicker Hardness Tester to determine the hardness value. Data analysis was performed using One Way Anova and Post-Hoc LSD tests.There is a significant difference in the value of composite resin hardness in the entire study group. The highest hardness value was found in the group with the application of glycerin and without immersion, while the lowest value was found in the group without the application of glycerin and immersion in 120 minutes.

Efek Air Kelapa Hijau (Cocos Nucifera Linn Var. Viridis) Sebagai Obat Kumur Terhadap Perubahan pH Saliva Anak Usia 12 Tahun Rabella Guspia Zhafirah; Roedy Budirahardjo; Raditya Nugroho
Pustaka Kesehatan Vol 7 No 3 (2019): Volume 7 No.3, 2019
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v7i3.10714

Latar belakang: Masalah utama kesehatan gigi dan mulut pada anak ialah karies gigi. Karies gigi merupakan suatu penyakit yang kompleks, yang diawali dengan adanya asam yang akan menghancurkan mineral-mineral gigi. Asam yang diproduksi mempengaruhi mineral gigi sehingga menjadi sensitif pada pH rendah. Ketika pH turun menjadi di bawah 5,5, proses demineralisasi menjadi lebih cepat dari remineralisasi. Hal ini menyebabkan lebih banyak mineral gigi yang luluh dan membuat lubang pada gigi. Berbagai upaya dilakukan untuk mengurangi masalah gigi ini dengan meningkatkan pH saliva yang bersifat asam. Salah satu cara yang digunakan adalah kontrol plak yaitu secara mekanik dan kimiawi Tujuan: Mengetahui efek penggunaan air kelapa hijau (Cocos nucifera Linn Var. Viridis) sebagai obat kumur terhadap pH saliva anak usia 12 tahun. Metode: Penelitian ini menggunakan eksperimental laboratoris dengan rancangan Pretest-Posttest Eksperimental : Controlled-Group Design dengan metode purposive sampling di Pondok pesantren Nurul Islam Jember sebanyak 27 orang. Hasil dan Kesimpulan: Hasil penelitian menunjukkan bahwa setelah berkumur dengan air kelapa hijau (Cocos nucifera Linn Var. Viridis) terdapat perubahan yaitu terjadi penurunan pH saliva anak usia 12 tahun. Kata Kunci: Perbedaan, pH Saliva, Air Kelapa Hijau, Chlorhexidine, Karies.

Perbedaan pH, Viskositas dan Volume Saliva setelah Berkumur dengan Obat Kumur Sintetik yang Mengandung Chlorhexidine dan Larutan Propolis pada Anak Usia 11-12 Tahun Shinta dinyanti; Roedy Budirahardjo; Raditya Nugroho
Pustaka Kesehatan Vol 7 No 3 (2019): Volume 7 No.3, 2019
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v7i3.10715

Dental caries is a process that begins with dissolution of enamel causing damage to the dental component by several factors, such as saliva. The occurrence of diseases in the oral cavity is prevented by the use of synthetic mouthwash containing chlorhexidie, but the using of synthetic compounds causes mutagenic effects on the oral cavity, so it needs alternative by using natural ingredients that have antibacterial power and smaller side effects. The purpose of the study was to investigate the difference of pH, viscosity and volume saliva after gargling with synthetic mouthwash containing of chlorhexidine and propolis solution for children age 11-12 years in Pondok Pesantren Nurul Islam Antirogo Jember. This study used pra-experimental One Shoot Case Study with total sampling in Pondok pesantren Nurul Islam Antirogo Jember. As many 25 students were recruited for the study. The result showed that after gargling with propolis solution can be increase of pH saliva, decrease of viscosity saliva and increase of volume saliva for children age 11-12 years in Pondok Pesantren Nurul Islam Antirogo Jember.

Pengaruh Lama Distres Kronis Terhadap Perubahan Jumlah Sel Osteoklas Pada Tulang Alveolar Tikus Sprague dawley (The Effect of Chronic Distres Duration on Osteoclast Changes in Alveolar Bone of Sprague dawley Rats) Cicik Khilar Rizqi; Happy Harmono; Raditya Nugroho
Pustaka Kesehatan Vol 4 No 1 (2016)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Chronic distress is a psycho-physiological reaction of stimuli that disrupt homoestasis which cause damage to the body, one of kind is an electrical shock stressor. The distress can increase catecholamines level in considerable amounts and allegedy it produce pro-inflamatory cytokines. Cytokine will affect the Hypoyhalamic pituitary adrenal (HPA) axis to produce cortisol and also act as negative control of cytokines. In this assumption, cytokines can increase bond of osteoclast formation key mediator which is RANKL with its receptor (RANK) to produce osteoclasts. This study was an experimental laboratories research using 8 weeks old Sprague dawley rats with body weight 200-250 gram as samples to observe increase of the number of osteoclasts in the alveolar bone. A total of 32 samples were divided into 4 groups: the rats group exposed with electrical shock stressor for 0, 7, 14, and 28 days. Then samples were processed by tissue processing until it’s ready to observe using an electron microscope with 400x magnification. The results showed that there were significant differences (p <0.05) between groups. It is concluded that the number of osteoclasts alveolar bone osteoclast were increased in Sprague dawley rats that experienced chronic distress. Keywords: stress/distress, cathecolamines, cytokine, cortisol,osteoclast

Frekuensi Kegagalan Pengisian Saluran Akar dengan Teknik Preparasi Step Back pada Gigi Berakar Ganda di Rumah Sakit Gigi dan Mulut Universitas Jember 2011-2016 (The Frequency of Failure Root Canal Filling with Step Back PreparationTechnique on Multiple Ro Melisa Novitasari; Raditya Nugroho; Sulistiyani .
Pustaka Kesehatan Vol 5 No 2 (2017)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Background: Most of root canal preparation use step back technique which form the root canal. Thecool lateral condensation technique is a standard technique taught to students, which has the highestcure rate between 87% - 94% and a root canal filling was ended by 0-2 mm from the radiographic apex,which the underfilling cure rate between 68% - 77% and the overfilling cure rate between 75% -76%.Some research suggests that the molars teeth had the highest frequency of failure in root canal filling.Objective: To identify the frequency of failure in root canal filling with preparation step back techniqueson a double-rooted teeth Dental Hospital of the University of Jember from 2011 until 2016. Methods: Thedesign of this study is a descriptive by gathering medical records. The radiograph pictures of doublerootedteeth which underfilling and overfilling were evaluated by researcher, data was calculated withmanual furthermore data was displayed in tables and graphics. Results and Conclusions: Teeth withunderfilling of 68 teeth larger than teeth that overfilled a total of 18 teeth. The maxillary teeth with themost underfilling are the teeth 14 and the tooth mandibular 46 where as the most overfilled teeth in themaxilla are the teeth 26 and the mandibular is the tooth 46. The percentage of root canal treatmentunderfilling and overfilling is 27.3% Overall number of patients with multiple root canal treatment.Keywords: lateral condensation, overfilling, step back, underfilling

The application of lesion sterilization and tissue repair 3Mix-MP for treating rat's dental pulp tissue Raditya Nugroho; Ananta Tantri Budi; Sri Kunarti
Dental Journal (Majalah Kedokteran Gigi) Vol. 48 No. 1 (2015): March 2015
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Lesion sterilization and tissue repair (LSTR) 3Mix-MP are three broad-spectrum antibiotics, including metronidazole, ciprofloxacin and minocycline are mixed with propylene glycol or macrogol. There is the possibility ofthe healing process that marked proliferation ofnew blood vessels and proliferation offibroblasts in the treatment ofirreversible pulpitis by pulp capping LSTR 3MixMP because of the principle of the method LSTR 3Mix-MP is to kill bacteria. Purpose: The purpose of this study to prove the effect of LSTR 3Mix-MP on chronic inflammation and the healing process in rat dental pulp tissue in vivo. Methods: Rattus norvegicus anaesthetized by using ketamine and xylazine dissolved in sterile isotonic saline solution (0.2 ml/50gr mm) on the upper right thigh. Cavity preparation class I to perforation by using a low speed tapered diamond round bur. In the treatment group, rats were treated 3Mix-MP at a dose of10 mg and then covered with glass ionomer cement for 7 days on the pulp that has been opened for 3 days. The control group treated with saline irrigation on the pulp that has been opened for 3 days. Rats were killed after seven days, and then made preparations pulp tissue to count the number oflymphocytes, macrophages, plasma cells, blood vessels, and fibroblasts Results: There is an increase in the average number ofmacrophage cells, plasma, and fibroblasts; and decreased lymphocytes and blood vessels in the treated group exposure LSTR 3Mix-MP. Conclusion:LSTR 3Mix-MP can reduce chronic inflammation process and enhance the healing process in rat dental pulp tissue.

Effects of glycerin application on the hardness of nanofilled composite immersed in tamarind soft drinks Titis Mustikaningsih Handayani; Raditya Nugroho; Lusi Hidayati; Dwi Warna Aju Fatmawati; Agus Sumono
Dental Journal (Majalah Kedokteran Gigi) Vol. 52 No. 2 (2019): June 2019
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v52.i2.p95-99

Background: Loss of tooth structure is a consideration in the performance of restorative treatment involving nanofilled composite resins. Material polymerization factors and water absorption can affect the hardness of composite resins. Imperfect polymerization producing an oxygen inhibited layer (OIL) and causing water absorption can even compromise the hardness of nanofilled composite resins. Tamarind soft drink, on the other hand, has an acidic pH that compromises the hardness of nanofilled composite resins. Purpose: This study aimed to reveal the effects of glycerin application on the hardness of nanofilled composite resins immersed in tamarind soft drinks. Methods: The research constituted a laboratory experiment using 24 nanofilled composite resin samples with diameters of 5mm or 2mm, divided into six groups, namely: Group G, Group G AS 60, Group G AS 120, Group TG, Group TG AS 60, and Group TG AS 120. Glycerin was applied to the surfaces of three groups before curing, while the other three groups were not treated with glycerin. Finishing was subsequently conducted on all samples using a highspeed handpiece and superfine finishing bur, before they were polished with a low speed handpiece. The samples were then divided into specific groups, namely: a group with a 120-minute immersion time, a group with a 60-minute immersion time, and a group which was not immersed and maintained at a temperature of 37oC. Each sample was tested at three points using a Vickers hardness tester (VHT). Results: The results showed that the groups with glycerin had a higher hardness level than those groups. In addition, the non-immersed groups had a higher hardness level than those groups which were immersed. The one-way ANOVA test results confirmed that there was a statistically significant difference (p<0.05) between all groups. Conclusion: The application of glycerin to nanofilled composite resins immersed in tamarind soft drinks can increase their hardness levels.

Perbandingan efektivitas enzim bromelain dan enzim papain terhadap degradasi jaringan karies dentin sebagai agen chemo-mechanical caries removalComparison of the effectiveness of bromelain and papain enzymes on the degradation of dentinal caries tissue as chemo-mechanical caries removal agents Johan Al-Falah; Berlian Prihatiningrum; Raditya Nugroho
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 34, No 1 (2022): April 2022
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkg.v34i1.34457

Pendahuluan: Metode pembersihan karies menggunakan handpiece dan bur terbukti masih memiliki banyak kekurangan menyangkut kecemasan dan rasa takut terutama pada anak-anak. Chemo-mechanical caries removal (CMCR) berbasis enzim protease merupakan metode alternatif dalam mengatasi kekurangan dari metode sebelumnya. Tujuan dari penelitian ini adalah menganalisis perbandingan efektivitas pemberian gel enzim bromelain konsentrasi 10% dan pemberian enzim papain selama 2 menit terhadap degradasi jaringan karies, bahwa diharapkan gel enzim bromelain dapat menjadi pertimbangan sebagai alternatif bahan CMCR. Metode: Penelitian ini merupakan penelitian eksperimental laboratoris dalam desain pre-test and post-test control group, dengan aplikasi gel bromelain konsentrasi 10% pada kelompok B dan enzim papain (BRIX3000®)pada kelompok P, pada sampel gigi premolar permanen rahang atas dengan karies dentin kelas I G.V. Black selama 2 menit, dengan kelompok K sebagai kontrol. Hasil: Hasil didapatkan berupa gambaran scanning electron microscope (SEM) dari permukaan jaringan karies akibat aplikasi kedua gel enzim protease. Rata-rata kedalaman degradasi jaringan karies dentin pada kelompok kontrol, kelompok enzim bromelain konsentrasi 10%, dan kelompok enzim papain berurutan 28,25 µm, 42,08 µm, dan 40,82 µm. Berdasarkan uji statistik perbedaan antara dua kelompok, kelompok perlakuan enzim protease memiliki perbedaan terhadap kelompok kontrol dengan nilai Sig.(2-tailed) kurang dari 0,05, sedangkan antara kelompok enzim bromelain dan kelompok enzim papain dengan nilai 0,856, yang berarti keduanya tidak berbeda secara signifikan. Simpulan: Aplikasi enzim bromelain ekstrak buah nanas konsentrasi 10% dalam penelitian ini memiliki efektivitas yang setara dengan aplikasi enzim papain (BRIX3000®) selama 2 menit dalam menghasilkan degradasi jaringan karies pada dentin, sehingga bisa menjadi alternatif bahan CMCR.Kata kunci: enzim bromelain konsentrasi 10%; enzim papain; Chemo-mechanical Caries Removal (CMCR); degradasi jaringan karies dentin; scanning electron microscope ABSTRACTIntroduction: The caries removal method using a handpiece and bur is proven to have many shortcomings regarding anxiety and fear, especially in children. Chemo-mechanical caries removal (CMCR) based on protease enzymes is an alternative method to overcome the shortcomings of the previous method. The purpose of this study was to analyse the effectiveness of 10% concentration of bromelain and papain enzyme application for 2 minutes on the caries tissue degradation. It is hoped that bromelain enzyme gel can be considered as an alternative CMCR material. Methods: This research was a laboratory experimental study in pre-test and post-test control group design, with application of 10% bromelain gel in group B and papain enzyme (BRIX3000®) in group P, on samples of maxillary permanent premolars with dentinal caries class I G.V. Black for 2 minutes, with group K as control. Results: The results were obtained in the form of a scanning electron microscope image of the carious tissue surface due to the application of the two protease enzyme gels. The average depth of dentin caries degradation in the control group, the 10% bromelain enzyme group, and the papain enzyme group were 28.25 µm, 42.08 µm, and 40.82 µm. Based on the statistical test of the difference between the two groups, the protease enzyme treatment groups had a significant difference compared to the control group with a Sig. (2-tailed) value of less than 0.05, while between the bromelain enzyme group and the papain enzyme group had a value of 0.856, which means the two were not different significantly. Conclusion: The application of the 10% concentration of bromelain enzyme in pineapple extract for 2 minutes in this study had an effectiveness equivalent to the application of the papain enzyme (BRIX3000® product) for 2 minutes in producing the degradation of carious tissue in dentin, so it could be an alternative material for CMCR.Keywords: 10% concentration of bromelain enzyme; papain enzyme; Chemo-mechanical Caries Removal (CMCR); dentin caries tissue degradation, scanning electron microscope

Efektivitas air perasan pulpa kakao 50% dalam membersihkan smear layer pada dinding saluran akar gigi The effectiveness of 50% cocoa pulp juice on removing smear layer of root canal dentin Disya Dwi Maulidiyah; Sri Lestari; Raditya Nugroho; Supriyadi Supriyadi; Dwi Warna Aju Fatmawati
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 33, No 3 (2021): Desember 2021
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkg.v33i3.34435

Pendahuluan: Smear layer merupakan lapisan tipis berisi substansi organik dan anorganik yang keberadaannya dapat menghalangi penetrasi medikamen ke tubuli dentin, sehingga harus dihilangkan dengan bahan irigasi saluran akar yaitu EDTA 17%. Bahan alami yang dapat digunakan sebagai alternatif dalam membersihkan smear layer pada dinding saluran akar adalah air perasan pulpa kakao 50% karena mengandung berbagai senyawa asam dan saponin. Tujuan penelitian menganalisis kemampuan air perasan pulpa kakao (Theobroma cacao L.) 50% dalam membersihkan smear layer pada dinding saluran akar Metode: Penelitian ini merupakan penelitian experimental laboratoris dengan rancangan penelitian the posttest only control group design. Terdapat dua kelompok uji yaitu air perasan pulpa kakao 50% dan EDTA 17%, masing-masing adalah 4 buah. Sampel yang digunakan adalah gigi insisif sapi yang direndam dalam larutan salin. Sampel dipotong mahkotanya setinggi servikal gigi dan di preparasi saluran akarnya dengan teknik konvensional menggunakan file no 45-80 dengan panjang kerja 21 mm, dirigasi dengan air perasan pulpa kakao 50% dan EDTA 17% tiap pergantian instrumen. Bagian yang diamati dengan Scanning Electron Microscope (SEM) 2000x adalah 1/3 tengah akar gigi bukal. Hasil pemotretan SEM dilakukan perhitungan skor menurut sistem skoring Hülsmann dan dilakukan analisis data dengan uji statistik Mann Whitney U Test. Hasil: Hasil penelitian dan analisis data didapatkan p=0,495 yang menunjukan bahwa tidak terdapat perbedaan bermakna antara keduanya. Kedua kelompok memiliki rerata skor 2 yang artinya sebagian orifis tubuli dentin terbuka dan terdapat sedikit smear layer. Simpulan: Air perasan pulpa kakao 50% mampu membersihkan smear layer saluran akar yang sebanding/setara dengan EDTA 17%.Kata kunci: air perasan pulpa kakao; bahan irigasi saluran akar; smear layerABSTRACTIntroduction: Smear layer is a thin layer containing organic and inorganic substances whose presence can prevent the penetration of the medicament into the dentinal tubules, so it must be removed with root canal irrigation material, namely 17% EDTA. Natural ingredients that can be used to clean the smear layer on the root canal wall are 50% cocoa pulp juice because it contains various acidic compounds and saponins. Therefore, the study aimed to analyze the ability of 50% cocoa (Theobroma cacao L.) pulp juice to clean the smear layer on the root canal walls. Methods: This study was an experimental laboratory study with the posttest only control group design. There were two test groups, namely 50% cocoa pulp juice and 17% EDTA, which were four pieces. The sample used was a bovine incisor immersed in a saline solution. The samples were crowned at the teeth' cervical level, and the root canals were prepared using conventional techniques using file no. 45-80 with a working length of 21 mm, irrigated with 50% cocoa pulp juice and 17% EDTA at each instrument change. The part observed with a Scanning Electron Microscope (SEM) 2000x was the middle 1/3 of the buccal tooth root. The results of the SEM observation were calculated according to the Hülsmann scoring system, and data were analyzed using the Mann Whitney U Test statistical test. Results: The results of the study and data analysis obtained p = 0.495, which indicates that there is no significant difference between the two. Both groups had a mean score of 2, meaning that most of the dentinal tubular orifices are open, and there is a thin smear layer. Conclusions: 50% cocoa pulp juice was able to clean the root canal smear layer, equivalent to 17% EDTA.Keywords: cocoa pulp juice; root canal irrigation agents; smear layer

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