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All Journal STOMATOGNATIC- Jurnal Kedokteran Gigi ODONTO Dental Journal Dental Journal (Majalah Kedokteran Gigi) Jurnal Kedokteran Gigi Universitas Padjadjaran Jurnal Material Kedokteran Gigi Mandala of Health : A Scientific Journal Odonto dental journal
Rinawati Satrio
Bagian Ilmu Penyakit Mulut, Kedokteran Gigi, Fakultas Kedokteran, Universitas Jenderal Soedirman
Dentistry Health Professions Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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Isolasi dan karakterisasi bakteri kariogenik pada pasien di Rumah Sakit Gigi dan Mulut Universitas Jenderal Soedirman Rinawati Satrio; Supriyati Supriyati; Fadli Ashar; Salwa Az-Zahra; Dwi Nur Indah Sari; Meylida Ichsyani
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 35, No 1 (2023): April 2023
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkg.v35i1.41439

Abstract

ABSTRAKPendahuluan: Karies gigi adalah hilangnya struktur kimia penyusun gigi yang disebabkan oleh interaksi kompleks antara mikroorganisme rongga mulut pada plak gigi, diet dan faktor host. Pencegahan karies dapat dilakukan dengan manipulasi bakteri yang diarahkan pada bakteri kariogenik dominan yang diisolasi dari karies. Penelitian ini bertujuan untuk mengisolasi dan mengkarakterisasi bakteri kariogenik pada pasien yang terdiagnosis pulpitis di Rumah Sakit Gigi dan Mulut Universitas Jenderal Soedirman pada bulan Desember 2020 – Februari 2021. Metode: Penelitian observasional deskriptif ini dilakukan dengan consecutive sampling. Sampel penelitian diambil dari karies gigi dengan kedalaman dentin pada 5 pasien di RSGM Unsoed yang terdiagnosis pulpitis. Identifikasi dilakukan dengan menumbuhkan bakteri pada media agar darah, Mannitol Salt Agar (MSA), dan Man Rogosa Sharpe Agar (MRSA) kemudian dilakukan pengamatan morfologi, perhitungan koloni bakteri, pewarnaan Gram dan uji-uji biokimia. Hasil : Jenis bakteri yang dapat diidentifikasi dari karies adalah Staphylococcus aureus (80%), Staphylococcus intermedius (40%), Coagulase Negative Staphylococcus (60%), Lactobacillus sp (80%) dan Lactobacillales Gram positif (20%). Simpulan: Didapatkan isolat yang diduga bakteri Staphylococcus aureus, Staphylococcus intermedius, Coagulase Negative Staphylococcus (CoNS), Lactobacillus sp dan Lactobacillales Gram positif yang diisolasi dari karies gigi.Kata kunci: karies gigi, bakteri kariogenik, staphylococcus aureus, lactobacillus spIsolation and characterization of cariogenic bacteria in patient at Universitas Jenderal Soedirman Dental HospitalABSTRACTIntroduction: Dental caries is the loss of the chemical structure of the teeth that is caused by complex interactions between oral microorganisms on dental plaque, diet and host factors. Caries prevention can be done by manipulation of bacteria directed at the dominant cariogenic bacteria isolated from caries. This study aims to isolate and characterize cariogenic bacteria in patients at the Universitas Jendral Soedirman Dental Hospital from Desember 2020 until Februari 2021. Methods: This descriptive observational study was conducted with consecutive sampling. Samples were taken from dental caries at dentine depth in 5 patients at Universitas Jenderal Soedirman Dental Hospital who were diagnosed with pulpitis. Identification was done by growing bacteria on blood agar media, Mannitol Salt Agar (MSA) and Man Rogosa Sharpe Agar (MRSA) then morphological observations, bacterial colony counting, Gram staining and biochemical tests were done. Results: The types of bacteria that can be identified from caries are Staphylococcus aureus (80%), Staphylococcus intermedius (40%), Coagulase Negative Staphylococcus (60%), Lactobacillus sp. (80%) and Gram positive Lactobacilllales (20%). Conclusion: We found isolates suspected to be Staphylococcus aureus, Staphylococcus intermedius, Coagulase Negative Staphylococcus (CoNS), Lactobacillus sp and Gram positive Lactobacilllales isolated from dental caries.Keywords: dental caries, cariogenic bacteria, staphylococcus aureus, lactobacillus sp
PREHEATING EFFECT OF GIOMER’S SURFACE HARDNESS ON DIRECT RESTORATION Irfan Dwiandhono; Aris Aji Kurniawan; Rinawati Satrio; Setiadi Warata Logamarta; Muthiary Nitzschia Nur Iswary Winanto
Odonto : Dental Journal Vol 9, No 1 (2022): July 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (323.982 KB) | DOI: 10.30659/odj.9.1.95-99

Abstract

Background: Composite resin is a material that is widely used for caries restoration, enamel abrasion, and aesthetic needs because of the good fit with teeth. Restorative materials that release fluoride ions may prolong the therapeutic and protective effects of the enamel. Nowadays, composite resins have been developed a new hybrid restorative material, named giomer. Giomer containing pre-reacted glass-ionomer (PRG) which can release and recharge fluoride ions. Composite resins have a disadvantage, a presence of shrinkage during polymerization, which can cause gaps between the tooth surface and composite resin. Preheating is carried out before irradiation with conventional oven. Preheating makes the composite resin stronger, reduces viscosity makes it easier to adapt and apply, and improves mechanical properties. This study aims to determine the effect of preheating on surface hardness.Method: The research sample used is 27 pieces cylinder-shaped with 5 mm diameter and 2 mm depth. The number of treatments was divided into 3 treatment groups. The treatment groups were divided based on the preheating treatment temperature of 37°C, 60°C, and control group. The surface hardness test was carried out using Vicker's microhardness tester.Result: The statistical One Way ANOVA test showed a significant result between the groups (p<0,05). LSD test (Least Significance Difference test) showed a significant result between the treatment temperature of 37°C, 60°C, and control group (p<0,05).Conclusion: The result of the giomer surface hardness test with preheating showed a higher result than the control group. The preheating group with a temperature of 60°C had the highest result.
Co-Authors A Haris Budi Widodo Amilia Ramadhani Anindita Laksitasari Annida Fatiya Zahra Ario Ditto Primandaru Aris Aji Kurniawan Aris Aji Kurniawan Aris Aji Kurniawan Christiana Cahyani Prihastuti Dwi Nur Indah Sari Endang Yunia Ekawati Fadli Ashar Fanni Kusuma Djati Irfan Dwiandhono Irfan Dwiandhono Irfan Dwiandhono Luqman Mahadika Cahyono Maulina Triani Meylida Ichsyani Muthiary Nitzschia Nur Iswary Winanto Muthiary Nitzschia Nur Iswary Winanto Pascalis Adhi Kurniawan Primarizka Iswara Laksmi Puspaningdyah Pramudyanaswari Rakhmawati Rakhmawati Ridhofar Akbar Khusnul Abdillah Ryana Budi Purnama Ryana Budi Purnama Salwa Az-Zahra Setiadi Warata Logamarta Setiadi Warata Logamarta Supriyati Supriyati Tuti Sri Suhesti Widyaningsih, Pratiwi Nur