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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Teknologi Pertanian Jurnal Teknologi Pertanian (Agricultural Technology Journal)
Zuheid Noor
Staf Pengajar Fakultas Teknologi Pertanian, Universitas Gajah Mada, Yogyakarta
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT

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Hypoglycemic Effect Determination of Soybean, Soybean Protein Fraction and Tempe in Diabetic Rats Wulan, Siti Narsito; Astuti, Mary; Marsono, Y.; Noor, Zuheid
Jurnal Teknologi Pertanian Vol 3, No 2 (2002)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (196.93 KB)

Abstract

Hypoglicemic effect of soybean, soybean protein fraction and tempe was determined in alloxan-induced diabetic rats.  Blood glucose and urinary glucose level were measured to determine this effect.Soybean protein fraction had the highest hypoglicemic property.  It lowered blood glucose level from 37.35 to 5.48 mg/dl/day/g carbohydrate consumed (85%).  Tempe showed lower hypoglycemic effect than soybean  protein fraction.  Blood glucose level decreased 51%, from 31.57 to 15.59 mg/dl/day/g carbohydrate consumed.  Soybean had the lowest hypoglycemic effect.  It lowered blood glucose level from 47.47 to 27.70 mg/dl/day/g carbohydrate consumed (42%).  Urinary glucose level had the same pattern as blood glucose level.Keywords :  Hypoglicemic effect, blood glucose level, soybean, tempe
ISOLASI DAN AKITIVITAS SPESIFIK ENZIM LIPASE INDIGENOUS BIJI KENARI Djarkasi, G. S. Suhartati; Raharjo, Sri; Noor, Zuheid
Jurnal Teknologi Pertanian (Agricultural Technology Journal Vol 8, No 1 (2017): Jurnal Teknologi Pertanian (Teta)
Publisher : Fakultas Pertanian, Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/jteta.8.1.2017.16349

Abstract

In general, plant oil resources has lypase enzyme which can hidrolyze oil to free fatty acids. The free fatty acid formed relatively easier to be oxidazed than that of in the form of triglycerides. The aims of this research are to investigate the specific activity of endogenous lypase enzyme and its optimum temperature. The protein was extracted by ammonium sulphate, dialysed, then fractionated through Sephadex G-100.The research proceeds with measurement activities of endogenous enzyme extracted from canarium kernel. From the research konwn that activities of lypase endogenous from Canarium indicum and Canarium vulgare are similar. It’s known that the more purification factor (PF) attained the higher specific activity (SA) achieved. Crude extract for Canarium indicum with FP=1 gives the SA=0.033 μmol/mg. When the PF is increased become 4.98 with saturation 70 % sodium sulphate, the SA value become 0.16 μmol/mg. Then, purification with sephadex G-100 for column chromatography cause the PF increase to 55.54 and the SA is 1.83 μmol/mg. In addition, for Canarium vulgare the higher PF give the more SA. Crude extract for Canarium vulgare with FP=1 gives the SA=0,033 μmol/mg. When the PF increase becomes 4.57 with saturation 70 % sodium sulphate, the SA value is 0.17 μmol/mg. Then, purification with sephadex G-100 for column chromatography, the PF increase to 51.13 and the SA is 1.94 μmol/mg. The optimum temperature of endogenous lipase from Canarium indicum is similar to lipase endogenous from Canarium vulgare, i.e 40°C. The free fatty acid content of Canarium indicum and Canarium vulgare kernel are 0.19±0.06% and 0.20±0.04%, respectively. After drying, free fatty acid content of Canarium indicum and Canarium vulgare kernel increase to 0.30±0.04% and 0.37±0.06%, respectively. The increase of free fatty acid has positive correlation with lipase activities.
Aktivitas Trypsin Inhibitor Berbagai Varietas Biji Kedelai (Glycine max L.) dan Perubahannya Selama Perkecambahan Biji dari Varietas Terbaik Kanetro, Bayu; Noor, Zuheid; Indrati, Retno
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 1 (2007): February 2007
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v12i1.2529

Abstract

This study investigated the activity of trypsin inhibitor (TI) of some soybean (Glycine max L.) varieties and the change in TI activity during seed germination of the best variety. The research was aimed to determine the best variety of soybean and germination time based on the highest TI activity. There were 5 varieties of soybeans, Paderman, Argomulyo, Kaba, Sinabung, and Ijen. The best variety of soybean was Sinabung as shown by the highest TI activity. The soybean of Sinabung variety was germinated for 6 various germination times at 12, 24, 36, 48, 60 and 72 hr. The result showed that the variation of germination time changed TI activity. TI activity decreased significantly after 36 hr of germination of soybeans. The best time of germination was 36 hr.
Co-Authors Bayu Kanetro Djarkasi, G. S. Suhartati Indrati, Retno Mary Astuti Siti Narsito Wulan Sri Raharjo Y. Marsono