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Calcium Alginate and Salt/Phosphate as Binding Agents in Restructured Lamb Setyawardani, Triana; Raharjo, Sri; sudarmadji, purnama
ANIMAL PRODUCTION Vol 3, No 1 (2001): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

A study on  restructurization of lamb meat using several binding agents were conducted. Objectives of the study were evaluate  effectivity of Ca–alginate, salt and phosphate as binding agent and their effect on physical properties of the restructured meat stored at -20⁰C for up to 12 weeks. Three binding agents were added to the restructured products, which include NaCl 0.3 %/ NTPP 0.3 %; alginate 0.5 %/Ca-lactate 0.5%; NaCl 0.3 % / NTPP 0.5 %/alginate 0.5% and no binding agent as a control. The products were evaluated at 0, 4, 8 and 12 weeks of storage. The result showed that treatment with alginate 0.5%/Ca-lactate 0.5% had the least purge loss value of 4.3±0.2%. The least cooking losses of 30.2±3.79% and the highest shear force 61.6±13.77 N. (Animal Production 3(1): 20-25 (2001)Key Words: Alginate/Ca-lactate, purge loss, cooking losses, shear  force.
Saatnya Indonesia Bangkit Melawan Aflatoksin Raharjo, Sri
JURNAL PANGAN Vol 18, No 1 (2009): PANGAN
Publisher : Perum BULOG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1705.817 KB) | DOI: 10.33964/jp.v18i1.215

Abstract

Di kalangan pimpinan pemerintahan dan politik nilai strategis dari penanganan aflatoksin dan dampaknya terhadap ketahanan dan kedaulatan pangan belum mendapatkan perhatian dan komitmen yang memadai. Perlu dipahami dan disadari bersama oleh seluruh warga bangsa Indonesia bahwa kegagalan dalam pengendalian terhadap cemaran aflatoksin pada hasil pertanian menimbulkan kerugian yang sangat besar, baik dalam jangka pendek maupun jangka panjang. Meskipun cemaran aflatoksin banyak dijumpai pada saat penanganan pascapanen namun untukmengatasinya diperlukan serangkaian langkah yang terfokus dan terkoordinasi dari segenap pihak. Untuk itu perlu diketahui sejauh mana kesiapan pengendalian aflatoksin secara nasional yang ada saat inidan langkah-langkah yang masih perlu untuk ditempuh. Unsur-unsur dalam sistem pengendalian aflatoksin terdiri dari lembaga yang memiliki otoritas atau kewenangan, peraturan dan perundangan yang mengatur tentang keamanan pangan, mekanisme pengelolaan dan penerapan standard keamanan pangan, penyedia layanan pengujian cemaran aflatoksin, cara berproduksi pada tingkat petani, rantai perdagangan hasil panen, dan industri pengolahan pakan dan pangan. Komitmen nasional perlu segera digalang dan diarahkan antara lain untuk mempromosikan kesadaran (awareness) tentang kerugian jangka pendek dan jangka panjang yang bisa ditimbulkan oleh cemaran khususnya aflatoksin, mengembangkan sistem dan tindakan pengendalian yang efektifpada tingkat on farm dan off farm, mengoptimalkan penyelenggaraan penelitian pengendalian aflatoksin, dan secara periodik dilakukan monitoring cemaran aflatoksin untuk keperluan perdagangan komiditas terkait.
The Effect of Size Reduction and Preparation Duration on The Antioxidant Activity of White Saffron (Curcuma mangga Val.) Pujimulyani, Dwiyati; Raharjo, Sri; Marsono, Y; Santoso, Umar
Journal of Food and Pharmaceutical Sciences Vol 1, No 1 (2013): J.Food Pharm.Sci (January-April)
Publisher : Fakultas Farmasi, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (403.451 KB) | DOI: 10.14499/jfps

Abstract

Food processing is usually conducted through preparation stages such as peeling, slicing, or cutting. The purpose of this research was to determine the correlation between the antioxidant activity and the total phenolic compounds of white saffron as affected by different level of size reduction and the duration of rhizome preparation at room temperature. White saffron rhizomes were peeled, washed, and cut into 1x1x1 cm3. The whole white saffron was sliced diagonally at thickness of 4 x 2 mm, and grated. The antioxidant activity of the white saffron was determined by radical scavenging activity using 2-2-diphenyl-1-picrylhydrazyl (DPPH) radical and by ferric reducing antioxidant power FRAP) method. The total phenol and total flavonoid contents were also determined at 2, 4, and 6 hours, respectively. The result showed that the antioxidant activities, total phenolic and flavonoid contents due to the preparation of whole white saffron for 6 hour are not statistically different compared to those of fresh white saffron. The preparation of white saffron sliced at 2 mm and grated for 2 hour showed a significant decrease in the antioxidant activities, compared to those of fresh white saffron.Keywords: white saffron, preparation duration, antioxidant activity, total phenol
ISOLASI DAN IDENTIFIKASI RHIZOPUS OLIGOSPORUS PADA BEBERAPA INOKULUM TEMPE Ari Sandhi Wipradnyadewi, Putu; S. Rahayu, Endang; Raharjo, Sri
Agrotekno Vol. 11, No. 2 Januari 2005
Publisher : Universitas Udayana

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Abstract

The research was aimed to isolate the fungi grown and to identify R. oligosporus inseveral tempe inoculum samples.Seventy microbes were isolated from tempe inoculum, which was consisted of 58greyish brown conidia, and 12 grey conidia isolates. All isolates were belong to genera ofRhizopus. Seven of them were selected based on their origin of tempe inoculum, conidiacolour, growth of mycelia, and conidia mass. Macroscopic and microscopic observationshowed that the selected isolates were R. oligosporus.
ISOLASI DAN AKITIVITAS SPESIFIK ENZIM LIPASE INDIGENOUS BIJI KENARI Djarkasi, G. S. Suhartati; Raharjo, Sri; Noor, Zuheid
Jurnal Teknologi Pertanian (Agricultural Technology Journal Vol 8, No 1 (2017): Jurnal Teknologi Pertanian (Teta)
Publisher : Fakultas Pertanian, Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/jteta.8.1.2017.16349

Abstract

In general, plant oil resources has lypase enzyme which can hidrolyze oil to free fatty acids. The free fatty acid formed relatively easier to be oxidazed than that of in the form of triglycerides. The aims of this research are to investigate the specific activity of endogenous lypase enzyme and its optimum temperature. The protein was extracted by ammonium sulphate, dialysed, then fractionated through Sephadex G-100.The research proceeds with measurement activities of endogenous enzyme extracted from canarium kernel. From the research konwn that activities of lypase endogenous from Canarium indicum and Canarium vulgare are similar. It’s known that the more purification factor (PF) attained the higher specific activity (SA) achieved. Crude extract for Canarium indicum with FP=1 gives the SA=0.033 μmol/mg. When the PF is increased become 4.98 with saturation 70 % sodium sulphate, the SA value become 0.16 μmol/mg. Then, purification with sephadex G-100 for column chromatography cause the PF increase to 55.54 and the SA is 1.83 μmol/mg. In addition, for Canarium vulgare the higher PF give the more SA. Crude extract for Canarium vulgare with FP=1 gives the SA=0,033 μmol/mg. When the PF increase becomes 4.57 with saturation 70 % sodium sulphate, the SA value is 0.17 μmol/mg. Then, purification with sephadex G-100 for column chromatography, the PF increase to 51.13 and the SA is 1.94 μmol/mg. The optimum temperature of endogenous lipase from Canarium indicum is similar to lipase endogenous from Canarium vulgare, i.e 40°C. The free fatty acid content of Canarium indicum and Canarium vulgare kernel are 0.19±0.06% and 0.20±0.04%, respectively. After drying, free fatty acid content of Canarium indicum and Canarium vulgare kernel increase to 0.30±0.04% and 0.37±0.06%, respectively. The increase of free fatty acid has positive correlation with lipase activities.
Oxidative Stability of Canarium Nut (Canarium indicum and Canarium vulgare) Oil during Storage at 30 and 40 0 C Suhartati Djarkasi; Sri Raharjo; Zuheid Noor; Slamet Sudarmadji
Jurnal Teknologi dan Industri Pangan Vol. 19 No. 2 (2008): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

The aims of this research were to study the effect of temperature and storage on the oxidative stability of crude and refined canarium nut oil extracted from the seeds of Canarium indicum and Canarium vulgare. The experiment was designed to include storage of two type of canarium nut oil at two different temperatures for up to 35 days. The oils (crude and refined) used had the similar condition. Parameter used for oxidative deterioration indicators were two peroxide value, TBARS (thiobarbituric acid reactive substances), and free fatty acid value. The result showed that refining oil can decrease component natural antioxidant of canarium oil cause more sensitive to the oxidation. Increased storage temperature can raise oxidation of crude and refined oils from both species Canarium. The peroxide values of crude and refined oils both Canarium indicum and Canarium vulgare stored at 30 °C were 2.17, 4.35, 3.36 and 3.77 meq O2/kg oil, respectively. When they were stored at 4?C the similar results were 6.21, 19.09, 8.12 and 17.23 meq O2/kg oil. Furthermore, TBARS value of crude and refined oils both for Canarium indicum and Canarium vulgare stored 30?C were 4.55, 7.78, 5.70 and 6.58 µmol MDA/kg oil. When they were stored at 40 °C the similar results were 9.99, 55.46, 12.46 and 43.62 µmol MDA/kg oil.Key words: Canarium nut oil, peroxide value, TBARS
The Role of Surfactant Micelles on the Partitioning of Antioxidant and the Oxidative Stability of Oil-in-Water Emulsion Posman Sibuea; Sri Raharjo; Umar Santoso; Zuheid Noor
Jurnal Teknologi dan Industri Pangan Vol. 19 No. 1 (2008): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

Lipid oxidation system in which the fat is dispersed as emulsion droplets is still not well understood, although a large number of food exist partially or entirely in the form of emulsions. Therefore, this study was intended to examine how surfactant micelles influence the partitioning of antioxidant and hydroperoxides and how it alter the oxidative stability of oil-in-water emulsion. To determine the ability of surfactant micelles upon the partitioning of antioxidant in oil-in-water emulsion, this type of emulsion, containing quercetin at 0, 25, 50, 75 and 100 ppm, were prepared with polyoxyethylene 100 stearyl ether (Brij 700) or polyoxyethylene sorbitan monolaurate (Tween 20) with acetate or phosphate buffer (pH 3.0 or 7.0). Structurally Birj 700 has 5 times longer polyoxyethylene groups than Tween 20. After preparation of the emulsion, surfactant micelles (0-2%) were incorporated into the continuous phase to give a final lipid concentration of 5%. Lipid oxidation rates, as determined by the formation of lipid hydroperoxides, decreased with increasing quercetin concentrations. At pH 3, the peroxide value was higher than that at pH 7. Brij 70 decreased production of lipid hydroperoxides from palm oil-in-water-emulsions compared to the emulsions stabilized by Tween 20. the result showed that solubilization of quercetin into the aqueous into the aqueous phase by Brij or Tween micelles did not alter the oxidative stability of palm oil-in-water emulsion, suggesting that surfactant micelle influenced the oxidation rate by mechanism other than antioxidant solubilization. Key word : Quercetin, surfactant micelles, and oxidative stability
Mechanism and Kinetic of Antiphotooxidation of Bixin in Metil Linoleat System Ardiyanto Pranata; Umar Santoso; Sri Raharjo; Haryadi .
Jurnal Teknologi dan Industri Pangan Vol. 17 No. 3 (2006): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

Bixin belongs to the carotenoid group, present in Bixa orellana tree. It has conjugated double bonds which plays a role as an antiphotooxidant. The objectives of this study were to analyze the quenching mechanisms and kinetics of bixin in photosensitized oxidation of methyl linoleate. The quenching mechanism and kinetic of bixin were studied by the steady-state kinetic method. Samples of 0, 0.25 x 10-5, 0.5 x 10-5, 0.75 x 10-5, 1.0 x 10-5, 2.0 x 10-5 and 3.0 x 10-5 M bixin were prepared in ethanol that also contained 0.03, 0.06, 0.09 and 0.12 M methyl linoleate and 11.36 x 10-6 M of erythrosine stored under fluorescent light (4000 lux) at room temperature for 2 hours. The extent of photosensitized oxidation of methyl linoleate was expressed as peroxide value. The steady-state kinetic study indicated that bixin quenched singlet oxygen and exhibited triplet sensitizer quenching mechanism (to minimize the erythrosine photosensitized oxidation of methyl linoleate). The total quenching rate constant of bixin was 7.7 x 109 M-1S-1. Key words: bixin, quencher, erythrosine, singlet oxygen
Mechanism and Kinetics of Quenching of Quercetin on Photosensitizing Effect of Synthetic Food Colorant Erythosine in Palm Oil Oxidation Posman sibuea; Sri Raharjo; Umar Santoso
Jurnal Teknologi dan Industri Pangan Vol. 16 No. 2 (2005): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

The effect 0, 200, 400, 600, 800 and 1000 ppm (wt/vol) quercetin on the singlet oxygen oxidation of palm oil in methylene chloride containing 100 ppm erythrosine, were studied during storage under 400 lux fluorecent light for 5 h by measuring peroxide value. Steady-state kinetic approximation was used to determine the quenching mechanism and quenching rate constant of quercetin in the erythrosine-sensitized photo oxidation of palm oil. Erythrosine greatly increased the As photo oxidation of palm oil, as was expected. Quercetin was extremely effective at minimizing erythrosine-sensitized photo oxidation of palm oil. As the concentration of quercetin increased from 0 to 200, 400, 600, 800 and 1000 ppm, the peroxide value of palm oils decreased significantly (p
The Influence of Particle size and Guluronic/Mannuronic Proportion of Alginate on Physical and Sensory Characteristics of Restructured Products from Soursop Sri Raharjo; Zaki Utama
Jurnal Teknologi dan Industri Pangan Vol. 14 No. 3 (2003): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

The application of restructuring technology is intended to add value for local fruits. Fresh and ripe sour sop fruits are frequently of having defective appearance, irregular shape and size, or showing signs of insect infestation which make the whole fruit is less acceptable by consumers. Previous study has indicated that sour sop fruit can be restructured into a fresh fruit product with acceptable sensory characteristics using calcium alginate gel system. This particular study was intended to determine the effect of alginate powder particles size and its guluronic/mannuronic proportion on the physical and sensorial properties of restructured sour sop. The restructured fruit product was evaluated based on its gel strength, pH colour, and sensory attributes which include taste, aroma, mouth feel, appearance, and hardness. Sour sop restructured by internal setting with coarse alginate powder (36 mesh) tend to have softer gel compared to the use of fine alginate powder (120 mesh). Different proportion of guluronic/mannuronic in the alginate used for the restructurization resulted in the same gel strength when calcium lactate powder was used. However, the use of encapsulated calcium lactate resulted in stronger gel with alginate containing higher proportion of mannuronic acid. The alginate particle size and proportion of guluronic/mannuronic content showed no significant difference in product colour and sensory attributes evaluated. Key words : Alginate, sour sop fruit, restructurization.