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All Journal Jurnal SainHealth Borneo Journal of Medical Laboratory Technology SPEKTA (Jurnal Pengabdian Kepada Masyarakat : Teknologi dan Aplikasi) Jurnal Pijar MIPA
Dheasy Herawati
Universitas Maarif Hasyim Latif
Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Energy Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics

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SENSITIVITAS DAN SPESIFIKASI DETEKSI Mycobacterium tuberculosis METODE TES CEPAT MOLEKULER GENEXPERT DENGAN PEMERIKSAAN MIKROSKOPIS Evy Ratnasari Ekawati; Didik Setio Prayitno; Dheasy Herawati
Jurnal SainHealth Vol 6, No 2 (2022): September 2022
Publisher : Faculty of Health Sciences Universitas Maarif Hasyim Latif

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51804/jsh.v6i2.2009.68-76

Abstract

The tuberculosis analysis may be made through microscopic strategies and the GeneXpert Molecular quick test. TCM is the modern-day discovery for the diagnosis via the semi-quantitative RTA technique. The rpoB gene hotspot place incorporated mycotuberculosis methods preparations automatically by extracting DNA in unmarried-use cartridges. This observation goal is to test the validity of GeneXpert TCM in comparison to microscopic methods. The form of research used is an experimental laboratory with qualitative assessment. The samples on this look had been 50 sputum samples from sufferers diagnosed with TB elderly 20-70 years with dilute situations to clarify the extent of sensitivity and specs for readings on GeneXpert and direct and 24-hour postpone the microscopic examination. The observation was conducted at the Jember Lung medical institution Laboratory. The consequences of the detection of Mycobacterium tuberculosis through the use of the TCM GeneXpert approach and direct and behind-schedule microscopic exams for twenty-four hours primarily based on Kruskal- Wallis statistical analysis acquired a significance price of zero.931 > a = 0.05, indicating no sizeable distinction between the techniques used. The distribution of suspected tuberculosis sufferers became extra male (66%) than women (34%). Most age companies infected with pulmonary TB are 40-60 years (42%). as a consequence, it may be concluded that each technique has the identical diagnostic capability in identifying Mycobacterium tuberculosis and may be used consistent with the desires, costs, and laboratory centers  Keywords: Tuberculosis, Mycobacterium tuberculosis, TCM GeneXpert, microscopic examination
Pengaruh Ekstrak Ethanol dan N-Hexan Daun Serai (Cymbopogon citratus) Terhadap Larva Nyamuk Aedes aegypti Gland Konay Octavianus; Abdul Chamid; Dheasy Herawati
Jurnal SainHealth Vol 7, No 1 (2023): Maret 2023
Publisher : Faculty of Health Sciences Universitas Maarif Hasyim Latif

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51804/jsh.v7i1.6847.35-40

Abstract

Dengue hemorrhagic fever (DHF) is a disease transmitted by the bite of a female Aedes aegypti mosquito infected with the dengue virus. The essential oil contained in lemon grass has antioxidant activity and contains active compounds that are toxic as larvicides. The purpose of this study was to determine the effectiveness, and the effective concentration of the Ethanol and N-Hexan extracts of lemon grass (Cymbopogon ciratus) leaves. The research subjects were 300 instar III Aedes aegypti larvae containing 25 larvae at each concentration with each concentration group namely 30 ppm, 35 ppm, 40 ppm, 45 ppm, and 50 ppm, which were observed for 1 hour, 3 hours, 6 hours, and 24 hours. From the results of the ethanol extract of citronella leaves (Cymbopogon ciratus) using graded concentrations Aedes aegypti larvae can die at high concentrations of 50 ppm and can kill 14 Aedes aegypti larvae. Whereas in the N-Hexan extract of lemongrass leaves (Cymbopogon ciratus) using graded concentrations Aedes aegypti larvae can die at high concentrations of 50 ppm which can kill 11 Aedes aegypti larvae. Lemongrass leaves (Cymbopogon ciratus) are effectively used as natural larvicides. Effective concentrations are 35 ppm, 40 ppm, 45 ppm, 50 ppm for 24 hours. Keywords: Aedes aegypti, Dengue Hemorrhagic Fever, Lemongrass (Cymbopogon ciratus) leaves.
Perbandingan Metode Membran Filter dan Metode Tabung Ganda Terhadap Kandungan Escherichia coli pada Air Bersih Lathifa Azkhiyati; Dheasy Herawati; Setyo Dwi Santoso; Esti Rizkiana Pratiwi; Elsa Mega Suryani
Jurnal SainHealth Vol 7, No 1 (2023): Maret 2023
Publisher : Faculty of Health Sciences Universitas Maarif Hasyim Latif

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51804/jsh.v7i1.6732.15-21

Abstract

Escherichia coli bacteria are bacteria used as indicators of contamination. Bacteriological water testing can use a Membrane Filtration (MF) and a Multiple Tube-Fermentation (MTF). This study aims to compare the membrane filtration and Multiple Tube-Fermentation methods on the presence of Escherichia coli in clean water. The two methods are compared to determine the percentage difference in the results of the two tests. The research methodology uses a description of observation. 20 samples of water boreholes were used and tested for the presence of Escherichia coli. Calculation of bacterial groups uses SNI standard references regulated by the Minister of Health of the Republic of Indonesia. The results showed that the average number of  Escherichia coli bacteria by the Multiple Tube-Fermentation method was less than the Membrane Filtration method. There is a difference in the percentage of the results of the two tests by 40%. Multiple Tube Fermentation is more sensitive than the Membrane Filtration method for detecting the presence of Escherichia coli in clean water.Keywords : Water, Escherichia coli, Membrane filtration, Multiple tube-fermentation
Perbandingan Metode Membran Filter dan Metode Tabung Ganda Terhadap Kandungan Escherichia coli pada Air Bersih Lathifa Azkhiyati; Dheasy Herawati; Setyo Dwi Santoso; Esti Rizkiana Pratiwi; Elsa Mega Suryani
Jurnal SainHealth Vol. 7 No. 1 (2023): Maret 2023
Publisher : Faculty of Health Sciences Universitas Maarif Hasyim Latif

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51804/jsh.v7i1.6732.15-21

Abstract

Escherichia coli bacteria are bacteria used as indicators of contamination. Bacteriological water testing can use a Membrane Filtration (MF) and a Multiple Tube-Fermentation (MTF). This study aims to compare the membrane filtration and Multiple Tube-Fermentation methods on the presence of Escherichia coli in clean water. The two methods are compared to determine the percentage difference in the results of the two tests. The research methodology uses a description of observation. 20 samples of water boreholes were used and tested for the presence of Escherichia coli. Calculation of bacterial groups uses SNI standard references regulated by the Minister of Health of the Republic of Indonesia. The results showed that the average number of  Escherichia coli bacteria by the Multiple Tube-Fermentation method was less than the Membrane Filtration method. There is a difference in the percentage of the results of the two tests by 40%. Multiple Tube Fermentation is more sensitive than the Membrane Filtration method for detecting the presence of Escherichia coli in clean water.Keywords : Water, Escherichia coli, Membrane filtration, Multiple tube-fermentation
Pengaruh Ekstrak Ethanol dan N-Hexan Daun Serai (Cymbopogon citratus) Terhadap Larva Nyamuk Aedes aegypti Gland Konay Octavianus; Abdul Chamid; Dheasy Herawati
Jurnal SainHealth Vol. 7 No. 1 (2023): Maret 2023
Publisher : Faculty of Health Sciences Universitas Maarif Hasyim Latif

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51804/jsh.v7i1.6847.35-40

Abstract

Dengue hemorrhagic fever (DHF) is a disease transmitted by the bite of a female Aedes aegypti mosquito infected with the dengue virus. The essential oil contained in lemon grass has antioxidant activity and contains active compounds that are toxic as larvicides. The purpose of this study was to determine the effectiveness, and the effective concentration of the Ethanol and N-Hexan extracts of lemon grass (Cymbopogon ciratus) leaves. The research subjects were 300 instar III Aedes aegypti larvae containing 25 larvae at each concentration with each concentration group namely 30 ppm, 35 ppm, 40 ppm, 45 ppm, and 50 ppm, which were observed for 1 hour, 3 hours, 6 hours, and 24 hours. From the results of the ethanol extract of citronella leaves (Cymbopogon ciratus) using graded concentrations Aedes aegypti larvae can die at high concentrations of 50 ppm and can kill 14 Aedes aegypti larvae. Whereas in the N-Hexan extract of lemongrass leaves (Cymbopogon ciratus) using graded concentrations Aedes aegypti larvae can die at high concentrations of 50 ppm which can kill 11 Aedes aegypti larvae. Lemongrass leaves (Cymbopogon ciratus) are effectively used as natural larvicides. Effective concentrations are 35 ppm, 40 ppm, 45 ppm, 50 ppm for 24 hours. Keywords: Aedes aegypti, Dengue Hemorrhagic Fever, Lemongrass (Cymbopogon ciratus) leaves.
Co-Authors Abdul Chamid Anton Yuntarso Didik Setio Prayitno Elsa Mega Suryani Esti Rizkiana Pratiwi Evi Dwi Aprilia Suryani Evy Ratnasari Ekawati Evy Ratnasari Ekawati Gland Konay Octavianus Istik Nuril Qomari Khoirul Ngibad Lathifa Azkhiyati Muhammad Sungging Pradana Muji Harsini Nurfadilah Nurfadilah Nurul Aziza Setyawan Setyawan Setyo Dwi Santoso Siti Nur Husnul Y. Soedaryo Soedaryo Titik Sundari Zelika Nur Jannah