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All Journal Hemera Zoa Jurnal Veteriner Journal of Veterinary and Animal Sciences
Arini Nurhandayani
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Biochemistry, Genetics & Molecular Biology Veterinary

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Journal : Jurnal Veteriner

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Kepekaan Telur Spesific Pathogen Free dan Clean Egg Terhadap Virus Flu Burung (SENSITIVITY OF SPESIFIC PATHOGEN FREE EGGS AND CLEAN EGG TO THE AVIAN INFLUENZA VIRUSES SUBTYPE H5N1) Gusti Ayu Yuniati Kencana; I Nyoman Suartha; Arini Nurhandayani; Muh Ramadhan
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Avian Influenza which is  in Indonesia  known as Flu Burung  is caused by the avian influenza virussubtype H5N1 (AIV-H5N1). Vaccination is one of the major strategies for preventing and eradicatingAIV-H5N1 in Indonesia. Several factors can affect the potential vaccine such as viral content and mediaused for the propagation of the virus. One of the media commonly used to propagate  the virus is pathogenspecific free (SPF) embryonated chicken eggs. However, as the SPF eggs production is limited and expensive,the use of clean embryonated chicken eggs as an alternative need to examined. This study aimed todetermine the sensitivity of SPF and clean embryonated chicken eggs to the AIV-H5N1. The virus usedwas seed avian influenza virus (A/ Chicken/West Java (Subang)/29/2007)  which haa previously werepropagated  in SPF eggs and the Clean Eggs. The virus titer was determined as Embryo infective Dose 50%(EID50) using Reed and Muench method. Sensitivity of SPF eggs and Clean Egg to the VAI-H5N1 wascompared using  Chi-square statistical analysis. The titers of Avian Influenza Virus subtype H5N1 were106.83EID50/0.1ml in SPF eggs and 106.17EID50/0.1 ml in the Clean Eggs. Statistical analysis showed that,the sensitivity of SPF Eggs and Egg Clean  for the propagation of the VAI-H5N1 was not significantlydifferent.
Identifikasi Secara Serologi Galur Virus Flu Burung Subtipe H5N1 Clade 2.1.3 dan Clade 2.3.2 pada Ayam Petelur (SEROLOGICAL IDENTIFICATION OF AVIAN INFLUENZA STRAIN VIRUS SUBTYPE H5N1 CLADE 2.1.3 AND CLADE 2.3.2 FROM LAYER) Aprilia Kusumastuti; Syamsidar .; Agustin Zaharia Paderi; Arini Nurhandayani; Gusti Ayu Yuniati Kencana
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of the study was to know avian influenza (AI) infection in field by using serology test in threemarketing area of AI vaccines. Haemagglutination inhibition methode was used in this test. There werefour antigen strains of AI subtype H5N1 clade 2.1.3 (AIstrainA/Chicken/West Java/PWT-WIJ/2006, AIstrain A/Chicken/Garut/BBVW-223/2007, AI strain A/Chicken/West Java-Nagrak/30/2007, and AI strainA/Chicken/Pekalongan/BBVW-208/2007) and 2 antigen strains of AI subtype H5N1 clade 2.3.2 (AI strainA/duck/Sukoharjo/BBVW-1428-9/2012 and AI strain A/duck/Sleman/BBVW-1463-10/2012) was used inthis study for HI test. The result presents that 93,33% chicken farms in three marketing area of PT. SanbioLaboratories have positive antibody titre to AI subtype H5N1 clade 2.1.3. This titre may be obtained fromAI clade 2.1.3 vaccination. From 15 samples, 92,86% are positive to AI subtype H5N1 clade 2.3.2A/duck/Sukoharjo/BBVW-1428-9/2012 and 92,31% are positive to A/duck/Sleman/BBVW-1463-10/2012 evenwithout AI clade 2.3.2 vaccination. This antibody titre may be obtained from AI clade 2.1.3 vaccine crossprotection or field infection.
Co-Authors . Syamsidar Agustin Zaharia Paderi Aprilia Kusumastuti Aprilia Kusumastuti Ardi Budi Prakoso Gusti Ayu Yunanti Kencana Gusti Ayu Yuniati Kencana I Nyoman Suartha Muh Ramadhan Steffi Ong Syamsidar . Syamsidar Syamsidar