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All Journal Jurnal Sain Veteriner Buletin Peternakan Jurnal Veteriner Indonesian Journal of Biotechnology
Siti Isrina Oktavia Salasia
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Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Veterinary Other

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Characterization of Haemolysin of Staphylococcus aureus Isolated from Food of Animal Origin Dwi Ariyanti; Siti Isrina Oktavia Salasia; Syarifudin Tato
Indonesian Journal of Biotechnology Vol 16, No 1 (2011)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (752.885 KB) | DOI: 10.22146/ijbiotech.7834

Abstract

Staphylococcus aureus is an important pathogen bacteria causing food poisoning and various infection in animals and humans. Haemolysin is one of the virulence factors of Staphylococcus aureus. The aims of the research were to characterize haemolysins of Staphylococcus aureus isolated from various food of animal origin, phenotypic- and genotypically. In the present study, eleven Staphylococcus aureus isolated from various food of animal origins from traditional markets and supermarkets in Yogyakarta, Sidoarjo, Jakarta, and Bandung were characterized for haemolysin, pheno- and genotypically. Characterization of haemolysin phenotypically based on haemolysis pattern of Staphylococcus aureus on sheep blood agar plate. Genes encoding hemolysin were amplified with specific primers by using polymerase chain reaction (PCR) technique. The results of the studies showed that Staphylococcus aureus on sheep blood agar plates revealed an alpha haemolysis pattern (18,18%), beta haemolysis (27,27%) and gamma haemolysis (54,55%). Based on amplification of the gene encoding haemolysin of Staphylococcus aureus with specific primers showed hla genes (81,81%), and hla combined with hlb genes (18,18%). The amplification of gene hla and hlb had a single amplicon with a size of approximately 534 bp and 833 bp, respectively. The haemolysin characteristics of Staphylococcus aureus from various food of animal origin could be used as important information to control staphylococcal food poisoning.Keywords : Staphylococcus aureus, haemolysin, PCR, food of animal origins
Respon Neutrofil, Adesi Pada Sel Epitel, Aglutinasi Eritrosit Terhadap Staphylococcus aureus : Kajian Hidrofobisitas In Vitro = Response of neutrophils, epithelial cells adhesion, erythrocytes agglutination of Staphyloco Khusnan .; Siti Isrina Oktavia Salasia
Jurnal Sain Veteriner Vol 24, No 1 (2006): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2690.953 KB) | DOI: 10.22146/jsv.345

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Staphylococcus aureus merupakan salah satu bakteri potensial sebagai penyebab utama mastitis pada sapi perah. Mastitis dapat menyebabkan kerugian ekonomi peternak akibat turunnya produksi susu. Infeksi bakteri dapat terjadi melalui kemampuan bakteri memasuki hospes, berkembang biak, merusak jaringan inang dan mampu bertahan dalam tubuh hospes. Penelitian ini bertujuan untuk mengetahui hubungan sifat hidrofobisitas S. aureus dan kemampuannya terhadap aglutinasi eritrosit, pelekatan dengan sel epitel dan kemampuan bertahan terhadap fagositosis sel polimorfonuklear. Dari 10 isolat S. aureus yang digunakan dalam penelitian ini, terdapat 8 isolat bersifat hidrofob dan 2 isolat bersifat hidrofil. Diantara isolat yang bersifat hidrofob terdapat 2 isolat mempunyai kemampuan mengaglutinasi eritrosit sapi perah, kambing, domba. Staphylococcus aureus yang bersifat hidrofob dan hemaglutinasi positif, lebih banyak melekat pada sel-sel epitel bukalis dan lebih banyak difagosit oleh sel-sel PMN dibanding isolat yang bersifat hidrofob tetapi hemaglutinasi negatif maupun isolat yang bersifat hidrofil. Isolat yang bersifat hidrofil tidak mampu mengaglutinasi eritrosit dan lebih sedikit melekat pada sel-sel epitel dan lebih sedikit difagosit oleh sel-sel PMN.
Efek Ekstrak Daun Bangun-Bangun (Coleus ambonicus, L.) pada Aktivitas Limfosit Tikus Putih Christin Marganingsih Santosa; Siti Isrina Oktavia Salasia
Jurnal Sain Veteriner Vol 22, No 2 (2004): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1392.143 KB) | DOI: 10.22146/jsv.483

Abstract

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Efek Ekstrak Air Daun Bangun-Bangun (Coleus ambonicus, L) pada Aktivitas Limfosit Tikus Putih Christin Marganingsih Santosa; Siti Isrina Oktavia Salasia
Jurnal Sain Veteriner Vol 22, No 2 (2004): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1392.143 KB) | DOI: 10.22146/jsv.484

Abstract

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Deteksi Protein Spesifik untuk Membedakan Daging Babi dengan Berbagai Macam Daging Spesies Lain Fikri Ardhani; Sigit Eko Prabowo; Afifah Nurul Hidayati; Siti Isrina Oktavia Salasia
Buletin Peternakan Vol 31, No 1 (2007): Buletin Peternakan Vol. 31 (1) Februari 2007
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v31i1.1216

Abstract

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Distribusi Gen Enterotoksin Staphylococcus aureus dari Susu Segar dan Pangan Asal Hewan Siti Isrina Oktavia Salasia; Khusnan -; Sugiyono -
Jurnal Veteriner Vol 10 No 3 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (150.453 KB)

Abstract

Staphylococcus aureus is a potential pathogen causing disease in human and animals due to severalvirulence factors. Staphylococcal enterotoxins which is responsible for foodborne disease is considered tobe one of the important virulence factor for the bacteria. The research was conducted to identify variousenterotoxin genes of S. aureus. Twenty three S. aureus isolates from milk cows (12 isolates) and foodanimal products (11 isolates) were used to study various enterotoxins genes (sea, seb, sec, sed, see, seg, seh,sei, and sej). The enterotoxins genes of S. aureus were investigated by using polymerase chain reaction(PCR) with specific primers. There were 3 isolates (13.04%) negative for staphylococcal enterotoxin genes.Twenty isolates (86.96%) harboured for one or more staphylococcal enterotoxin genes such as: sec (6 isolates/26.09%), see, seh for 1 isolate (4.35%), combination of 2 genes se (b,i), se (c,g), se (g,i) for 1 isolate of each(4.35%), se(c,e) for 2 isolates (8.70%), se(b,c) for 4 isolates (17.39%). Staphylococcal enterotoxin could bedetected in 3 combination genes of se(b,c,i), se(c,e,i), se(c,g,i) for 1 isolate of each (4.35%).
ISOLATION AND PHENOTIPICAL CHARACTERIZATION OF STAPHYLOCOCCUS AUREUS ISOLATED FROM SLAUGHTERED HOUSE WASTE AND CARCASS OF CHICKEN Khusnan -; Siti Isrina Oktavia Salasia; Sugiyono -
Jurnal Veteriner Vol 9 No 1 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1585.601 KB)

Abstract

Staphylococcus aureus is known as a potential agent that cause food poisoning. Chicken meats could be as vehicles of food poisoning cases while they contaminated by agents during slaughtered and meat processing as well as infected chickens by S. aureus itself. The aims of the research were to isolated and characterized of S. aureus from liquid wastes of chicken slaughtered house and washing water of chicken carcass. The characterization of S aureus based on mannitol salt agar (MSA), catalase and coagulation assay, production of hemolysins, hydrophobicity test, and the reaction of hemaglutination. Thirty seven samples used in this study were collected from 16 liquid wastes slaughtered chicken house and 21 samples from washing water of chicken carcasses.The research resulted 18 S. aureus isolated from liquid wastes slaughtered house (4 isolates) and from wahing water of chicken carcasses (14 isolates). All 18 isolates fermented mannitol, catalase and coagulase positive, contain of hydrophilic surfaces. Among 18 S. aureus isolates could agglutinate of rabbit erythrocytes for 55%, produced ?-hemolysis (12%) and ?-hemolysis (88%).
Co-Authors Afif Nurul Hidayati Christin Marganingsih Santosa Dwi Ariyanti Fikri Ardhani Khusnan k Sigit Eko Prabowo Sugiyono - Syarifudin Tato