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All Journal Herb-Medicine Journal Journal of Experimental and Clinical Pharmacy (JECP)
Ratna Widayati
Fakultas Kedokteran Universitas Palangka Raya, Palangka Raya
Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health

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UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL BIJI KAMANDRAH (Croton tilgium L.) Terhadap Pertumbuhan Salmonella typhi Dengan Metode Difusi Cakram (Kirby-Bauer) Revina Destiana Samputri; Angeline Novia Toemon; Ratna Widayati
Herb-Medicine Journal: Terbitan Berkala Ilmiah Herbal, Kedokteran dan Kesehatan Vol 3, No 3 (2020): Herb-Medicine Journal Oktober 2020
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/hmj.v3i3.6393

Abstract

ABSTRAKSalmonella typhi merupakan batang gram negatif, yang tidak memiliki spora, dapat bergerak dengan flagel peritrich, bersifat intraseluler dan anerob. Penyakit yang paling umum terjadi karena bakteri Salmonella typhiadalah demam tifoid. Kamandrah (Croton tigliumL.) merupakan suatu tanaman yang berasal dari suku Euphorbiaceae dapat digunakan sebagai antibakteri. Bertujuan untuk mengetahui apakah ekstrak etanol biji Kamandrah (Croton tiglium L.) dapat menghambat pertumbuhan bakteri Salmonella typhidengan metode difusi cakram Kirby-bauer. Penelitian ini merupakan true experiment designdengan rancangan penelitian Post test only control group design. Subjek penelitian menggunakan biakan bakteri Salmonella typhi.Penelitian ini menggunakan 7 kelompok perlakuan, yaitu konsentrasi 20%, 40%, 60%, 80%, 100% dan kontrol positif Kloramfenikol 30µg serta kontrol negatif dimethyl sulfoxide(DMSO) sebagai kontrol negatif. Metode yang digunakan untuk pengujian aktivitas antibakteri menggunakan metode difusi cakram Kirby-bauer. Analisis data menggunakan uji Kruskal-Wallisdalam aplikasi SPSS. Hasil bahwa biji Kamandrah (Croton tigliumL.) tidak dapat menghambat pertumbuhan Salmonella typhidengan rata-rata zona hambat konsentrasi 20% (0,00 mm), 40% (0,00 mm), 60% (0,00 mm), 80% (0,00 mm), dan 100% (0,00 mm). kontrol positif (28,2 mm) serta kontrol negatif (0,00 mm). Uji Kruskal -Wallis(P= 0,000). Ekstrak etanol biji Kamandrah (Croton tigliumL.) tidak dapat menghambat pertumbuhan Salmonella typhidan tidak terdapat konsentrasi paling efektif ekstrak etanol biji Kamandrah (Croton tigliumL.) yang dapat menghambat pertumbuhan bakteri Salmonella typhi.
Potential of Ciplukan Leaf Extract (Physalis angulata L.) Against Streptococcus pneumoniae Growth in Vitro Rakhman, Farizky; Widayati, Ratna; Turnip, Oktaviani Naulita; Fatmaria, Fatmaria; Hanasia, Hanasia
Journal of Experimental and Clinical Pharmacy (JECP) Vol 5, No 1 (2025): February 2025
Publisher : Poltekkes Kemenkes Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52365/jecp.v5i1.1314

Abstract

Pneumonia is a disease that infects the lungs, and antibiotics such as tetracycline show a high degree of resistance. Therefore, the use of alternative natural materials, such as ciplukan leaves (Physalis angulata L.), which the Dayak people commonly use to treat lung diseases, is necessary. This study aimed to prove that the extract of ciplukan leaves can inhibit Streptococcus pneumoniae by measuring the diameter of the clear zone and determining the Minimum Inhibitory Concentration (MIC). The extract was obtained by maceration using 96% ethanol, and then concentrations of 5%, 10%, 15%, 20%, and 25% were prepared. The positive control was levofloxacin 5 µg, and the negative control was DMSO 1%. The average inhibition zone diameters of the ciplukan leaf extract were 5% (0 mm), 10% (0.17 mm), 15% (0.45 mm), 20% (1.95 mm), 25% (2.67 mm). Antibacterial test using the disk diffusion method. From the Kruskal-Wallis and Mann-Whitney Post Hoc statistical test, a significance value of <0.05 was obtained. Therefore, There is an effect of ciplukan leaf extract on Streptococcus pneumoniae at concentrations of 15%, 20%, and 25%, with the MIC 15%.
Co-Authors Angeline Novia Toemon Fatmaria, Fatmaria Hanasia, Hanasia Rakhman, Farizky Revina Destiana Samputri Turnip, Oktaviani Naulita